Differentiation of a porcine reproductive and respiratory syndrome virus vaccine strain from North American field strains by restriction fragment length polymorphism analysis of ORF 5
Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA. The suitability of restriction fragment length polymorphism (RFLP) analysis for differentiating a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strain from ot...
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| Veröffentlicht in: | Journal of veterinary diagnostic investigation 1998-04, Vol.10 (2), p.140-144 |
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| container_title | Journal of veterinary diagnostic investigation |
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| creator | Wesley, R.D Mengeling, W.L Lager, K.M Clouser, D.F Landgraf, J.G Frey, M.L |
| description | Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA.
The suitability of restriction fragment length polymorphism (RFLP) analysis for differentiating a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strain from other North American field strains was investigated. Open reading frame 5 nucleotide sequence data of the vaccine virus, its parent strain VR-2332, and 22 other strains of PRRSV included in this study indicated that 3 restriction enzyme gel patterns characterize the vaccine virus and the parent strain genotype. The combined 3 RFLP patterns differentiate the vaccine and parent virus from other PRRSV strains. This test will be a valuable tool in epidemiologic studies and will be useful in identifying individual strains in cases of multistrain PRRSV infections. |
| doi_str_mv | 10.1177/104063879801000204 |
| format | Article |
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The suitability of restriction fragment length polymorphism (RFLP) analysis for differentiating a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strain from other North American field strains was investigated. Open reading frame 5 nucleotide sequence data of the vaccine virus, its parent strain VR-2332, and 22 other strains of PRRSV included in this study indicated that 3 restriction enzyme gel patterns characterize the vaccine virus and the parent strain genotype. The combined 3 RFLP patterns differentiate the vaccine and parent virus from other PRRSV strains. This test will be a valuable tool in epidemiologic studies and will be useful in identifying individual strains in cases of multistrain PRRSV infections.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/104063879801000204</identifier><identifier>PMID: 9576340</identifier><language>eng</language><publisher>Los Angeles, CA: J Vet Diagn Invest</publisher><subject>Alphaarterivirus ; analysis ; Animals ; Arterivirus ; Base Sequence ; classification ; Codon ; DNA Restriction Enzymes ; genetics ; genotype ; identification ; immunology ; live vaccines ; North America ; nucleotide sequences ; Open Reading Frames ; Phylogeny ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; porcine reproductive and respiratory syndrome ; Porcine Reproductive and Respiratory Syndrome - immunology ; Porcine Reproductive and Respiratory Syndrome - prevention & control ; Porcine respiratory and reproductive syndrome virus ; Porcine respiratory and reproductive syndrome virus - classification ; Porcine respiratory and reproductive syndrome virus - genetics ; Porcine respiratory and reproductive syndrome virus - immunology ; prevention & control ; restriction fragment length polymorphism ; RNA, Viral ; RNA, Viral - analysis ; Swine ; vaccines ; Viral Vaccines</subject><ispartof>Journal of veterinary diagnostic investigation, 1998-04, Vol.10 (2), p.140-144</ispartof><rights>1998 American Association of Veterinary Laboratory Diagnosticians</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c534t-2d6e9559abf72a101080ac0c2f68be994f3b865b3d32a5003d8c543f50eca0633</citedby><cites>FETCH-LOGICAL-c534t-2d6e9559abf72a101080ac0c2f68be994f3b865b3d32a5003d8c543f50eca0633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9576340$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wesley, R.D</creatorcontrib><creatorcontrib>Mengeling, W.L</creatorcontrib><creatorcontrib>Lager, K.M</creatorcontrib><creatorcontrib>Clouser, D.F</creatorcontrib><creatorcontrib>Landgraf, J.G</creatorcontrib><creatorcontrib>Frey, M.L</creatorcontrib><title>Differentiation of a porcine reproductive and respiratory syndrome virus vaccine strain from North American field strains by restriction fragment length polymorphism analysis of ORF 5</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA.
The suitability of restriction fragment length polymorphism (RFLP) analysis for differentiating a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strain from other North American field strains was investigated. Open reading frame 5 nucleotide sequence data of the vaccine virus, its parent strain VR-2332, and 22 other strains of PRRSV included in this study indicated that 3 restriction enzyme gel patterns characterize the vaccine virus and the parent strain genotype. The combined 3 RFLP patterns differentiate the vaccine and parent virus from other PRRSV strains. This test will be a valuable tool in epidemiologic studies and will be useful in identifying individual strains in cases of multistrain PRRSV infections.</description><subject>Alphaarterivirus</subject><subject>analysis</subject><subject>Animals</subject><subject>Arterivirus</subject><subject>Base Sequence</subject><subject>classification</subject><subject>Codon</subject><subject>DNA Restriction Enzymes</subject><subject>genetics</subject><subject>genotype</subject><subject>identification</subject><subject>immunology</subject><subject>live vaccines</subject><subject>North America</subject><subject>nucleotide sequences</subject><subject>Open Reading Frames</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>porcine reproductive and respiratory syndrome</subject><subject>Porcine Reproductive and Respiratory Syndrome - immunology</subject><subject>Porcine Reproductive and Respiratory Syndrome - prevention & control</subject><subject>Porcine respiratory and reproductive syndrome virus</subject><subject>Porcine respiratory and reproductive syndrome virus - classification</subject><subject>Porcine respiratory and reproductive syndrome virus - genetics</subject><subject>Porcine respiratory and reproductive syndrome virus - immunology</subject><subject>prevention & control</subject><subject>restriction fragment length polymorphism</subject><subject>RNA, Viral</subject><subject>RNA, Viral - analysis</subject><subject>Swine</subject><subject>vaccines</subject><subject>Viral Vaccines</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUctu1DAUjRColMIPICG8YjfUzyReVi0tSBWVgK6tG8ee8SiJg50Mypfxe73zEBskWNm653GPzi2Kt4x-ZKyqLhmVtBR1pWvKKKWcymfFOdNSrKQW5XP8I2G1Z7wsXuW8pVRxVbGz4kyrqhSSnhe_b4L3LrlhCjCFOJDoCZAxJhsGR5IbU2xnO4WdIzC0OMhjSDDFtJC8DG2KvSO7kOZMdmAPmjwlCAPxCJGvMU0bctW7FCzgLLiuPREyaZa93YTQYbFPsO4xB-ncsEbVGLulj2nchNzjbuiWHPI-3sO3W6JeFy88dNm9Ob0XxePtpx_Xn1f3D3dfrq_uV1YJOa14WzqtlIbGVxwY1lRTsNRyX9aN01p60dSlakQrOChKRVtbJYVX1FnAbsVF8eHoi0X8nDGu6UO2rutgcHHOBruXXJTlf4myLqVWukYiPxJtijkn582YQg9pMYya_VnN32dF0buT-9z0rv0jOd0R8csjnmHtzDbOCQvL_3Y85d2E9eZXSM7kHroO_bnZ7tqAUm7Ywfr9keghGlinkM3jd06ZoFxTWSHjCeZvxUw</recordid><startdate>19980401</startdate><enddate>19980401</enddate><creator>Wesley, R.D</creator><creator>Mengeling, W.L</creator><creator>Lager, K.M</creator><creator>Clouser, D.F</creator><creator>Landgraf, J.G</creator><creator>Frey, M.L</creator><general>J Vet Diagn Invest</general><general>SAGE Publications</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>7X8</scope></search><sort><creationdate>19980401</creationdate><title>Differentiation of a porcine reproductive and respiratory syndrome virus vaccine strain from North American field strains by restriction fragment length polymorphism analysis of ORF 5</title><author>Wesley, R.D ; Mengeling, W.L ; Lager, K.M ; Clouser, D.F ; Landgraf, J.G ; Frey, M.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-2d6e9559abf72a101080ac0c2f68be994f3b865b3d32a5003d8c543f50eca0633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Alphaarterivirus</topic><topic>analysis</topic><topic>Animals</topic><topic>Arterivirus</topic><topic>Base Sequence</topic><topic>classification</topic><topic>Codon</topic><topic>DNA Restriction Enzymes</topic><topic>genetics</topic><topic>genotype</topic><topic>identification</topic><topic>immunology</topic><topic>live vaccines</topic><topic>North America</topic><topic>nucleotide sequences</topic><topic>Open Reading Frames</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>porcine reproductive and respiratory syndrome</topic><topic>Porcine Reproductive and Respiratory Syndrome - immunology</topic><topic>Porcine Reproductive and Respiratory Syndrome - prevention & control</topic><topic>Porcine respiratory and reproductive syndrome virus</topic><topic>Porcine respiratory and reproductive syndrome virus - classification</topic><topic>Porcine respiratory and reproductive syndrome virus - genetics</topic><topic>Porcine respiratory and reproductive syndrome virus - immunology</topic><topic>prevention & control</topic><topic>restriction fragment length polymorphism</topic><topic>RNA, Viral</topic><topic>RNA, Viral - analysis</topic><topic>Swine</topic><topic>vaccines</topic><topic>Viral Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wesley, R.D</creatorcontrib><creatorcontrib>Mengeling, W.L</creatorcontrib><creatorcontrib>Lager, K.M</creatorcontrib><creatorcontrib>Clouser, D.F</creatorcontrib><creatorcontrib>Landgraf, J.G</creatorcontrib><creatorcontrib>Frey, M.L</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wesley, R.D</au><au>Mengeling, W.L</au><au>Lager, K.M</au><au>Clouser, D.F</au><au>Landgraf, J.G</au><au>Frey, M.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differentiation of a porcine reproductive and respiratory syndrome virus vaccine strain from North American field strains by restriction fragment length polymorphism analysis of ORF 5</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>1998-04-01</date><risdate>1998</risdate><volume>10</volume><issue>2</issue><spage>140</spage><epage>144</epage><pages>140-144</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA.
The suitability of restriction fragment length polymorphism (RFLP) analysis for differentiating a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strain from other North American field strains was investigated. Open reading frame 5 nucleotide sequence data of the vaccine virus, its parent strain VR-2332, and 22 other strains of PRRSV included in this study indicated that 3 restriction enzyme gel patterns characterize the vaccine virus and the parent strain genotype. The combined 3 RFLP patterns differentiate the vaccine and parent virus from other PRRSV strains. This test will be a valuable tool in epidemiologic studies and will be useful in identifying individual strains in cases of multistrain PRRSV infections.</abstract><cop>Los Angeles, CA</cop><pub>J Vet Diagn Invest</pub><pmid>9576340</pmid><doi>10.1177/104063879801000204</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of veterinary diagnostic investigation, 1998-04, Vol.10 (2), p.140-144 |
| issn | 1040-6387 1943-4936 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Alphaarterivirus analysis Animals Arterivirus Base Sequence classification Codon DNA Restriction Enzymes genetics genotype identification immunology live vaccines North America nucleotide sequences Open Reading Frames Phylogeny Polymerase Chain Reaction Polymorphism, Restriction Fragment Length porcine reproductive and respiratory syndrome Porcine Reproductive and Respiratory Syndrome - immunology Porcine Reproductive and Respiratory Syndrome - prevention & control Porcine respiratory and reproductive syndrome virus Porcine respiratory and reproductive syndrome virus - classification Porcine respiratory and reproductive syndrome virus - genetics Porcine respiratory and reproductive syndrome virus - immunology prevention & control restriction fragment length polymorphism RNA, Viral RNA, Viral - analysis Swine vaccines Viral Vaccines |
| title | Differentiation of a porcine reproductive and respiratory syndrome virus vaccine strain from North American field strains by restriction fragment length polymorphism analysis of ORF 5 |
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