Cloning, sequencing, and expression of a cDNA encoding rat liver mitochondrial carnitine palmitoyltransferase II

We report the isolation and characterization of a full-length cDNA encoding rat liver carnitine palmitoyltransferase II (CPT II). Beginning with the purified protein CNBr fragments were generated and sequenced. Corresponding oligonucleotides were used to screen a rat liver cDNA library constructed i...

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Veröffentlicht in:	The Journal of biological chemistry 1990-06, Vol.265 (18), p.10720-10725
Hauptverfasser:	WOELTJE, K. F, ESSER, V, WEIS, B. C, SEN, A, COX, W. F, MCPHAUL, M. J, SLAUGHTER, C. A, FOSTER, D. W, MCGARRY, J. D
Format:	Artikel
Sprache:	eng
Schlagworte:	Acyltransferases - genetics Amino Acid Sequence Animals Base Sequence Biological and medical sciences Carnitine O-Palmitoyltransferase - genetics Carnitine O-Palmitoyltransferase - isolation & purification carnitine palmitoyltransferase Cell Line Cloning, Molecular DNA - genetics Fundamental and applied biological sciences. Psychology Gene Expression Gene Library Genes. Genome Isoenzymes - genetics Isoenzymes - isolation & purification liver Male mitochondria Mitochondria, Liver - enzymology Molecular and cellular biology Molecular genetics Molecular Sequence Data Plasmids Protein Biosynthesis Protein Conformation Rats Rats, Inbred Strains Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic Transfection
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