Role of membrane phospholipids and glycolipids in the Vero cell surface receptor for rubella virus
Membrane receptors for rubella virus (RV) in Vero cells were studied by means of two different approaches: (i) by enzyme treatment of the whole cell membrane and (ii) by testing the ability of isolated plasma membrane molecules to compete with cells for virus binding. The replication of RV was studi...
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| Veröffentlicht in: | Medical microbiology and immunology 1990-01, Vol.179 (2), p.105-114 |
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| creator | MASTROMARINO, P CIOE, L RIETI, S ORSI, N |
| description | Membrane receptors for rubella virus (RV) in Vero cells were studied by means of two different approaches: (i) by enzyme treatment of the whole cell membrane and (ii) by testing the ability of isolated plasma membrane molecules to compete with cells for virus binding. The replication of RV was studied with both indirect immunofluorescence assay and molecular hybridization techniques. Phospholipases A2 and C digestion of cells greatly reduced the infectivity by the virus, pointing towards the involvement of lipid structures as receptor sites for RV. Furthermore, susceptibility of Vero cells to virus infection was also reduced after beta-N-acetyl-D-glucosaminidase, alpha-glucosidase and beta-galactosidase treatment, suggesting that carbohydrate residues may participate in a complex cellular receptor structure for RV. When the major membrane lipids were examined separately for their ability to inhibit viral infectivity, several phospholipids (phosphatidylserine, phosphatidylinositol, phosphatidylethanolamine, phosphatidylcholine, sphingomyelin) and glycolipids (gangliosides, lactosylceramide, cerebroside sulphate) showed a strong neutralizing activity, confirming the role of membrane lipid moiety in the cell surface receptor for RV. |
| doi_str_mv | 10.1007/bf00198531 |
| format | Article |
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The replication of RV was studied with both indirect immunofluorescence assay and molecular hybridization techniques. Phospholipases A2 and C digestion of cells greatly reduced the infectivity by the virus, pointing towards the involvement of lipid structures as receptor sites for RV. Furthermore, susceptibility of Vero cells to virus infection was also reduced after beta-N-acetyl-D-glucosaminidase, alpha-glucosidase and beta-galactosidase treatment, suggesting that carbohydrate residues may participate in a complex cellular receptor structure for RV. When the major membrane lipids were examined separately for their ability to inhibit viral infectivity, several phospholipids (phosphatidylserine, phosphatidylinositol, phosphatidylethanolamine, phosphatidylcholine, sphingomyelin) and glycolipids (gangliosides, lactosylceramide, cerebroside sulphate) showed a strong neutralizing activity, confirming the role of membrane lipid moiety in the cell surface receptor for RV.</description><identifier>ISSN: 0300-8584</identifier><identifier>EISSN: 1432-1831</identifier><identifier>DOI: 10.1007/bf00198531</identifier><identifier>PMID: 2192246</identifier><identifier>CODEN: MMIYAO</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Biological and medical sciences ; cell membranes ; Dose-Response Relationship, Drug ; Enzymes ; Fundamental and applied biological sciences. 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The replication of RV was studied with both indirect immunofluorescence assay and molecular hybridization techniques. Phospholipases A2 and C digestion of cells greatly reduced the infectivity by the virus, pointing towards the involvement of lipid structures as receptor sites for RV. Furthermore, susceptibility of Vero cells to virus infection was also reduced after beta-N-acetyl-D-glucosaminidase, alpha-glucosidase and beta-galactosidase treatment, suggesting that carbohydrate residues may participate in a complex cellular receptor structure for RV. When the major membrane lipids were examined separately for their ability to inhibit viral infectivity, several phospholipids (phosphatidylserine, phosphatidylinositol, phosphatidylethanolamine, phosphatidylcholine, sphingomyelin) and glycolipids (gangliosides, lactosylceramide, cerebroside sulphate) showed a strong neutralizing activity, confirming the role of membrane lipid moiety in the cell surface receptor for RV.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>cell membranes</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycolipids - pharmacology</subject><subject>Membrane Lipids - pharmacology</subject><subject>Microbiology</subject><subject>Peptide Hydrolases</subject><subject>Phospholipids - pharmacology</subject><subject>Receptors, Virus</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>RNA, Viral - analysis</subject><subject>Rubella virus - genetics</subject><subject>Rubella virus - growth & development</subject><subject>Rubella virus - metabolism</subject><subject>Vero Cells - drug effects</subject><subject>Vero Cells - metabolism</subject><subject>Viral Plaque Assay</subject><subject>Virology</subject><subject>Virus Replication</subject><issn>0300-8584</issn><issn>1432-1831</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1LwzAYxoMoc35cvAu56EGo5rNJjjqcCgNB1GtJ0sRV2rUmrbD_3ox1evTwEJLnx8P7PgHgDKNrjJC4MR4hrCSneA9MMaMkw5LifTBFFKFMcskOwVGMn4kSOUETMCFYEcLyKTAvbe1g62HjGhP0ysFu2cakuuqqMkK9KuFHvba7e7WC_dLBdxdaaF1dwzgEr62DwVnX9W2APikMJnkafldhiCfgwOs6utPxPAZv8_vX2WO2eH54mt0uMsuQ6jOqlBG5tqU0hjkuS5ELraWx1KqckVxrxKXQLC8x4sykR8JKrgyRwiPJCD0Gl9vcLrRfg4t90VRxM2Paqh1iIZSkXCLxL4i5UAojmsCrLWhDG2NwvuhC1eiwLjAqNs0Xd_Nd8wk-H1MH07jyFx2rTv7F6Otode1T2baKf4mKYcrTv_0AG-aKsQ</recordid><startdate>19900101</startdate><enddate>19900101</enddate><creator>MASTROMARINO, P</creator><creator>CIOE, L</creator><creator>RIETI, S</creator><creator>ORSI, N</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19900101</creationdate><title>Role of membrane phospholipids and glycolipids in the Vero cell surface receptor for rubella virus</title><author>MASTROMARINO, P ; CIOE, L ; RIETI, S ; ORSI, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-399b76acd8bb4e58d767aa8bc3c96426aa0587a46d1054bc9624d59b287f08423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>cell membranes</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycolipids - pharmacology</topic><topic>Membrane Lipids - pharmacology</topic><topic>Microbiology</topic><topic>Peptide Hydrolases</topic><topic>Phospholipids - pharmacology</topic><topic>Receptors, Virus</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>RNA, Viral - analysis</topic><topic>Rubella virus - genetics</topic><topic>Rubella virus - growth & development</topic><topic>Rubella virus - metabolism</topic><topic>Vero Cells - drug effects</topic><topic>Vero Cells - metabolism</topic><topic>Viral Plaque Assay</topic><topic>Virology</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MASTROMARINO, P</creatorcontrib><creatorcontrib>CIOE, L</creatorcontrib><creatorcontrib>RIETI, S</creatorcontrib><creatorcontrib>ORSI, N</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Medical microbiology and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MASTROMARINO, P</au><au>CIOE, L</au><au>RIETI, S</au><au>ORSI, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of membrane phospholipids and glycolipids in the Vero cell surface receptor for rubella virus</atitle><jtitle>Medical microbiology and immunology</jtitle><addtitle>Med Microbiol Immunol</addtitle><date>1990-01-01</date><risdate>1990</risdate><volume>179</volume><issue>2</issue><spage>105</spage><epage>114</epage><pages>105-114</pages><issn>0300-8584</issn><eissn>1432-1831</eissn><coden>MMIYAO</coden><abstract>Membrane receptors for rubella virus (RV) in Vero cells were studied by means of two different approaches: (i) by enzyme treatment of the whole cell membrane and (ii) by testing the ability of isolated plasma membrane molecules to compete with cells for virus binding. The replication of RV was studied with both indirect immunofluorescence assay and molecular hybridization techniques. Phospholipases A2 and C digestion of cells greatly reduced the infectivity by the virus, pointing towards the involvement of lipid structures as receptor sites for RV. Furthermore, susceptibility of Vero cells to virus infection was also reduced after beta-N-acetyl-D-glucosaminidase, alpha-glucosidase and beta-galactosidase treatment, suggesting that carbohydrate residues may participate in a complex cellular receptor structure for RV. When the major membrane lipids were examined separately for their ability to inhibit viral infectivity, several phospholipids (phosphatidylserine, phosphatidylinositol, phosphatidylethanolamine, phosphatidylcholine, sphingomyelin) and glycolipids (gangliosides, lactosylceramide, cerebroside sulphate) showed a strong neutralizing activity, confirming the role of membrane lipid moiety in the cell surface receptor for RV.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>2192246</pmid><doi>10.1007/bf00198531</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences cell membranes Dose-Response Relationship, Drug Enzymes Fundamental and applied biological sciences. Psychology Glycolipids - pharmacology Membrane Lipids - pharmacology Microbiology Peptide Hydrolases Phospholipids - pharmacology Receptors, Virus Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains RNA, Viral - analysis Rubella virus - genetics Rubella virus - growth & development Rubella virus - metabolism Vero Cells - drug effects Vero Cells - metabolism Viral Plaque Assay Virology Virus Replication |
| title | Role of membrane phospholipids and glycolipids in the Vero cell surface receptor for rubella virus |
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