Cyclic AMP and Butyrate Modulate Melatonin Synthesis in Y79 Human Retinoblastoma Cells
: Melatonin is synthesized by cultured Y79 human retinoblastoma cells and is secreted into the medium. Activity of the two key enzymes involved in the synthesis of melatonin, N‐acetyltransferase (NAT) and hydroxyindole‐O‐methyl‐transferase (HIOMT), are present in retinoblastoma cells. The activity o...
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| Veröffentlicht in: | Journal of neurochemistry 1990-07, Vol.55 (1), p.208-214 |
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| creator | Wiechmann, Allan F. Kyritsis, Athanassios P. Fletcher, R. Theodore Chader, Gerald J. |
| description | : Melatonin is synthesized by cultured Y79 human retinoblastoma cells and is secreted into the medium. Activity of the two key enzymes involved in the synthesis of melatonin, N‐acetyltransferase (NAT) and hydroxyindole‐O‐methyl‐transferase (HIOMT), are present in retinoblastoma cells. The activity of these enzymes and the resulting synthesis and release of melatonin are modulated by the addition of a cyclic AMP analogue and butyrate to the culture medium. Melatonin levels increase dramatically over control levels after the addition of dibutyryl cyclic AMP (dbcAMP), whereas melatonin levels decrease after butyrate treatment. HIOMT activity is inhibited by both dbcAMP and butyrate, and NAT activity is stimulated by both of these differentiating agents, suggesting that the rise in melatonin levels in response to dbcAMP is the result of increased activity of NAT, whereas the decline in melatonin levels in response to butyrate may be due to a drop in HIOMT activity. Melatonin synthesis is dose‐ and time‐dependent, and the effect of dbcAMP is readily reversible, whereas the effect of butyrate does not appear to be reversible. These effects probably reflect basic differences in the regulatory mechanisms of the inducing agents. |
| doi_str_mv | 10.1111/j.1471-4159.1990.tb08840.x |
| format | Article |
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Theodore ; Chader, Gerald J.</creator><creatorcontrib>Wiechmann, Allan F. ; Kyritsis, Athanassios P. ; Fletcher, R. Theodore ; Chader, Gerald J.</creatorcontrib><description>: Melatonin is synthesized by cultured Y79 human retinoblastoma cells and is secreted into the medium. Activity of the two key enzymes involved in the synthesis of melatonin, N‐acetyltransferase (NAT) and hydroxyindole‐O‐methyl‐transferase (HIOMT), are present in retinoblastoma cells. The activity of these enzymes and the resulting synthesis and release of melatonin are modulated by the addition of a cyclic AMP analogue and butyrate to the culture medium. Melatonin levels increase dramatically over control levels after the addition of dibutyryl cyclic AMP (dbcAMP), whereas melatonin levels decrease after butyrate treatment. HIOMT activity is inhibited by both dbcAMP and butyrate, and NAT activity is stimulated by both of these differentiating agents, suggesting that the rise in melatonin levels in response to dbcAMP is the result of increased activity of NAT, whereas the decline in melatonin levels in response to butyrate may be due to a drop in HIOMT activity. Melatonin synthesis is dose‐ and time‐dependent, and the effect of dbcAMP is readily reversible, whereas the effect of butyrate does not appear to be reversible. These effects probably reflect basic differences in the regulatory mechanisms of the inducing agents.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.1990.tb08840.x</identifier><identifier>PMID: 2162375</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acetylserotonin O-Methyltransferase - metabolism ; Arylamine N-Acetyltransferase - metabolism ; Biological and medical sciences ; Bucladesine - pharmacology ; Butyrates - pharmacology ; Butyric Acid ; Eye Neoplasms - metabolism ; Eye Neoplasms - pathology ; Fundamental and applied biological sciences. Psychology ; Hormones and neuropeptides. Regulation ; Humans ; Hydroxyindole ; Hypothalamus. Hypophysis. Epiphysis. Urophysis ; Melatonin ; Melatonin - biosynthesis ; Neoplasm Proteins - metabolism ; N‐Acetyltransferase ; O‐methyltransferase ; Pineal ; Retina ; Retinoblastoma ; Retinoblastoma - metabolism ; Retinoblastoma - pathology ; Tumor Cells, Cultured ; Vertebrates: endocrinology</subject><ispartof>Journal of neurochemistry, 1990-07, Vol.55 (1), p.208-214</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4308-4b71250e7a2a6a33e4b5958e5845a15d6a155249e5a50ac21bfdc419a27b1ee33</citedby><cites>FETCH-LOGICAL-c4308-4b71250e7a2a6a33e4b5958e5845a15d6a155249e5a50ac21bfdc419a27b1ee33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-4159.1990.tb08840.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-4159.1990.tb08840.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19264923$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2162375$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wiechmann, Allan F.</creatorcontrib><creatorcontrib>Kyritsis, Athanassios P.</creatorcontrib><creatorcontrib>Fletcher, R. Theodore</creatorcontrib><creatorcontrib>Chader, Gerald J.</creatorcontrib><title>Cyclic AMP and Butyrate Modulate Melatonin Synthesis in Y79 Human Retinoblastoma Cells</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: Melatonin is synthesized by cultured Y79 human retinoblastoma cells and is secreted into the medium. Activity of the two key enzymes involved in the synthesis of melatonin, N‐acetyltransferase (NAT) and hydroxyindole‐O‐methyl‐transferase (HIOMT), are present in retinoblastoma cells. The activity of these enzymes and the resulting synthesis and release of melatonin are modulated by the addition of a cyclic AMP analogue and butyrate to the culture medium. Melatonin levels increase dramatically over control levels after the addition of dibutyryl cyclic AMP (dbcAMP), whereas melatonin levels decrease after butyrate treatment. HIOMT activity is inhibited by both dbcAMP and butyrate, and NAT activity is stimulated by both of these differentiating agents, suggesting that the rise in melatonin levels in response to dbcAMP is the result of increased activity of NAT, whereas the decline in melatonin levels in response to butyrate may be due to a drop in HIOMT activity. Melatonin synthesis is dose‐ and time‐dependent, and the effect of dbcAMP is readily reversible, whereas the effect of butyrate does not appear to be reversible. These effects probably reflect basic differences in the regulatory mechanisms of the inducing agents.</description><subject>Acetylserotonin O-Methyltransferase - metabolism</subject><subject>Arylamine N-Acetyltransferase - metabolism</subject><subject>Biological and medical sciences</subject><subject>Bucladesine - pharmacology</subject><subject>Butyrates - pharmacology</subject><subject>Butyric Acid</subject><subject>Eye Neoplasms - metabolism</subject><subject>Eye Neoplasms - pathology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormones and neuropeptides. Regulation</subject><subject>Humans</subject><subject>Hydroxyindole</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>Melatonin</subject><subject>Melatonin - biosynthesis</subject><subject>Neoplasm Proteins - metabolism</subject><subject>N‐Acetyltransferase</subject><subject>O‐methyltransferase</subject><subject>Pineal</subject><subject>Retina</subject><subject>Retinoblastoma</subject><subject>Retinoblastoma - metabolism</subject><subject>Retinoblastoma - pathology</subject><subject>Tumor Cells, Cultured</subject><subject>Vertebrates: endocrinology</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkF1r1UAQhhex1GP1JwiLoHeJO_uRZL0QatBWaVW0FbxaJps5mEM-2mxCm39v0hPqZele7OzyvjPz8jD2GkQM83m3i0GnEGkwNgZrRTwUIsu0iG-fsM299JRthJAyUkLLZ-x5CDshINEJHLJDCYlUqdmw3_nk68rz4_MfHNuSfxyHqceB-HlXjvXdg-bStVXLf03t8JdCFfj8-ZNafjo22PKfNFRtV9QYhq5BnlNdhxfsYIt1oJdrPWKXnz9d5KfR2feTL_nxWeS1ElmkixSkEZSixASVIl0YazIymTYIpkzmy0htyaAR6CUU29JrsCjTAoiUOmJv93Ov-u56pDC4pgp-ToAtdWNwqc2UAaEfNIJJNMhsmfh-b_R9F0JPW3fVVw32kwPhFvpu5xbEbkHsFvpupe9u5-ZX65axaKi8b11xz_qbVcfgsd722Poq_N9gZaKtXEJ82PtuqpqmRyRwX7_lUmTqHzW0oAs</recordid><startdate>199007</startdate><enddate>199007</enddate><creator>Wiechmann, Allan F.</creator><creator>Kyritsis, Athanassios P.</creator><creator>Fletcher, R. Theodore</creator><creator>Chader, Gerald J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>199007</creationdate><title>Cyclic AMP and Butyrate Modulate Melatonin Synthesis in Y79 Human Retinoblastoma Cells</title><author>Wiechmann, Allan F. ; Kyritsis, Athanassios P. ; Fletcher, R. Theodore ; Chader, Gerald J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4308-4b71250e7a2a6a33e4b5958e5845a15d6a155249e5a50ac21bfdc419a27b1ee33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Acetylserotonin O-Methyltransferase - metabolism</topic><topic>Arylamine N-Acetyltransferase - metabolism</topic><topic>Biological and medical sciences</topic><topic>Bucladesine - pharmacology</topic><topic>Butyrates - pharmacology</topic><topic>Butyric Acid</topic><topic>Eye Neoplasms - metabolism</topic><topic>Eye Neoplasms - pathology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormones and neuropeptides. Regulation</topic><topic>Humans</topic><topic>Hydroxyindole</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>Melatonin</topic><topic>Melatonin - biosynthesis</topic><topic>Neoplasm Proteins - metabolism</topic><topic>N‐Acetyltransferase</topic><topic>O‐methyltransferase</topic><topic>Pineal</topic><topic>Retina</topic><topic>Retinoblastoma</topic><topic>Retinoblastoma - metabolism</topic><topic>Retinoblastoma - pathology</topic><topic>Tumor Cells, Cultured</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wiechmann, Allan F.</creatorcontrib><creatorcontrib>Kyritsis, Athanassios P.</creatorcontrib><creatorcontrib>Fletcher, R. Theodore</creatorcontrib><creatorcontrib>Chader, Gerald J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wiechmann, Allan F.</au><au>Kyritsis, Athanassios P.</au><au>Fletcher, R. Theodore</au><au>Chader, Gerald J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cyclic AMP and Butyrate Modulate Melatonin Synthesis in Y79 Human Retinoblastoma Cells</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1990-07</date><risdate>1990</risdate><volume>55</volume><issue>1</issue><spage>208</spage><epage>214</epage><pages>208-214</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: Melatonin is synthesized by cultured Y79 human retinoblastoma cells and is secreted into the medium. Activity of the two key enzymes involved in the synthesis of melatonin, N‐acetyltransferase (NAT) and hydroxyindole‐O‐methyl‐transferase (HIOMT), are present in retinoblastoma cells. The activity of these enzymes and the resulting synthesis and release of melatonin are modulated by the addition of a cyclic AMP analogue and butyrate to the culture medium. Melatonin levels increase dramatically over control levels after the addition of dibutyryl cyclic AMP (dbcAMP), whereas melatonin levels decrease after butyrate treatment. HIOMT activity is inhibited by both dbcAMP and butyrate, and NAT activity is stimulated by both of these differentiating agents, suggesting that the rise in melatonin levels in response to dbcAMP is the result of increased activity of NAT, whereas the decline in melatonin levels in response to butyrate may be due to a drop in HIOMT activity. Melatonin synthesis is dose‐ and time‐dependent, and the effect of dbcAMP is readily reversible, whereas the effect of butyrate does not appear to be reversible. These effects probably reflect basic differences in the regulatory mechanisms of the inducing agents.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>2162375</pmid><doi>10.1111/j.1471-4159.1990.tb08840.x</doi><tpages>7</tpages></addata></record> |
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| subjects | Acetylserotonin O-Methyltransferase - metabolism Arylamine N-Acetyltransferase - metabolism Biological and medical sciences Bucladesine - pharmacology Butyrates - pharmacology Butyric Acid Eye Neoplasms - metabolism Eye Neoplasms - pathology Fundamental and applied biological sciences. Psychology Hormones and neuropeptides. Regulation Humans Hydroxyindole Hypothalamus. Hypophysis. Epiphysis. Urophysis Melatonin Melatonin - biosynthesis Neoplasm Proteins - metabolism N‐Acetyltransferase O‐methyltransferase Pineal Retina Retinoblastoma Retinoblastoma - metabolism Retinoblastoma - pathology Tumor Cells, Cultured Vertebrates: endocrinology |
| title | Cyclic AMP and Butyrate Modulate Melatonin Synthesis in Y79 Human Retinoblastoma Cells |
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