Ubiquitinated conjugates are found in preparations of several plant viruses
Recently D. D. Dunigan, R. G. Dietzgen, J. E. Schoelz, and M. Zaitlin ( Virology 165, 310–312,1988) demonstrated that a small proportion of the subunits of tobacco mosaic virus particles were conjugated with the small protein ubiquitin. We have now detected ubiquitinated conjugates in immunoblots of...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1990-07, Vol.177 (1), p.352-356 |
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| creator | Hazelwood, D. Zaitlin, Milton |
| description | Recently D. D. Dunigan, R. G. Dietzgen, J. E. Schoelz, and M. Zaitlin (
Virology 165, 310–312,1988) demonstrated that a small proportion of the subunits of tobacco mosaic virus particles were conjugated with the small protein ubiquitin. We have now detected ubiquitinated conjugates in immunoblots of virion preparations of several other plant viruses, using anti-human ubiquitin antiserum. Based on their polyacrylamide gel migrations, plant virus-associated ubiquitin-immunoreactive proteins were considered to be possible virus structural protein-ubiquitin conjugates of the following viruses: barley stripe mosaic, brome mosaic, cowpea mosaic (two proteins), cowpea severe mosaic (two proteins), and satellite panicum mosaic. Ubiquitinated conjugates were not detected in immunoblots of preparations of cucumber mosaic virus and Cymbidium mosaic virus. The significance of ubiquitinated viral proteins remains to be determined. |
| doi_str_mv | 10.1016/0042-6822(90)90490-I |
| format | Article |
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Virology 165, 310–312,1988) demonstrated that a small proportion of the subunits of tobacco mosaic virus particles were conjugated with the small protein ubiquitin. We have now detected ubiquitinated conjugates in immunoblots of virion preparations of several other plant viruses, using anti-human ubiquitin antiserum. Based on their polyacrylamide gel migrations, plant virus-associated ubiquitin-immunoreactive proteins were considered to be possible virus structural protein-ubiquitin conjugates of the following viruses: barley stripe mosaic, brome mosaic, cowpea mosaic (two proteins), cowpea severe mosaic (two proteins), and satellite panicum mosaic. Ubiquitinated conjugates were not detected in immunoblots of preparations of cucumber mosaic virus and Cymbidium mosaic virus. The significance of ubiquitinated viral proteins remains to be determined.</description><subject>AUTOGRAPHA</subject><subject>AUTOGRAPHA CALIFORNICA</subject><subject>Biological and medical sciences</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunoblotting</subject><subject>INVERTEBRATE VIRUSES</subject><subject>Microbiology</subject><subject>Molecular Weight</subject><subject>Morphology, structure, chemical composition, physicochemical properties</subject><subject>Mosaic Viruses - analysis</subject><subject>PLANT VIRUSES</subject><subject>POLYHEDROSIS VIRUSES</subject><subject>Protein Binding</subject><subject>PROTEOLISIS</subject><subject>PROTEOLYSE</subject><subject>PROTEOLYSIS</subject><subject>Species Specificity</subject><subject>Ubiquitins - isolation & purification</subject><subject>Viral Proteins - isolation & purification</subject><subject>Virion - analysis</subject><subject>Virology</subject><subject>VIRUS DE INVERTEBRADOS</subject><subject>VIRUS DE LAS PLANTAS</subject><subject>VIRUS DES INVERTEBRES</subject><subject>VIRUS DES VEGETAUX</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1u1DAURi0EKkPhBRBI3oDoIuXaSZzxphKqgI6o1AXM2rpjX1euMnFqJyPx9jjMqOzY-Eff8eerw9g7AZcChPoM0MhKraX8pOFCQ6Oh2jxjKwFaVVA34jlbPSEv2aucH6Dcuw7O2JkUShZwxX5sd-FxDlMYcCLHbRwe5vtyzBwTcR_nwfEw8DHRiAmnEIfMo-eZDpSw52OPw8QPIc2Z8mv2wmOf6c1pP2fbb19_Xd9Ut3ffN9dfbivbtHKqtKrRyh1ivaxSi1pSJ8GjVi148MI6j0p2a-VqT9pR6xSi3aFtvSKQ9Tn7eOwdU3ycKU9mH7KlvsxCcc6m02u5ViAK2BxBm2LOibwZU9hj-m0EmMWhWQSZRZDRYP46NJvy7P2pf97tyT09Okkr-YdTjtli7xMONuR_3botrfXy_dsj5zEavE-F2f7UUGvooIRXx5CKqUOgZLINNFhyIZGdjIvh_1P-ARRPlvw</recordid><startdate>19900701</startdate><enddate>19900701</enddate><creator>Hazelwood, D.</creator><creator>Zaitlin, Milton</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900701</creationdate><title>Ubiquitinated conjugates are found in preparations of several plant viruses</title><author>Hazelwood, D. ; Zaitlin, Milton</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-963ac2baa3c2ba29132e720fa9650f0f1cdfa62786d3fe9de5d6aacbac5f6e023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>AUTOGRAPHA</topic><topic>AUTOGRAPHA CALIFORNICA</topic><topic>Biological and medical sciences</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunoblotting</topic><topic>INVERTEBRATE VIRUSES</topic><topic>Microbiology</topic><topic>Molecular Weight</topic><topic>Morphology, structure, chemical composition, physicochemical properties</topic><topic>Mosaic Viruses - analysis</topic><topic>PLANT VIRUSES</topic><topic>POLYHEDROSIS VIRUSES</topic><topic>Protein Binding</topic><topic>PROTEOLISIS</topic><topic>PROTEOLYSE</topic><topic>PROTEOLYSIS</topic><topic>Species Specificity</topic><topic>Ubiquitins - isolation & purification</topic><topic>Viral Proteins - isolation & purification</topic><topic>Virion - analysis</topic><topic>Virology</topic><topic>VIRUS DE INVERTEBRADOS</topic><topic>VIRUS DE LAS PLANTAS</topic><topic>VIRUS DES INVERTEBRES</topic><topic>VIRUS DES VEGETAUX</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hazelwood, D.</creatorcontrib><creatorcontrib>Zaitlin, Milton</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hazelwood, D.</au><au>Zaitlin, Milton</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ubiquitinated conjugates are found in preparations of several plant viruses</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1990-07-01</date><risdate>1990</risdate><volume>177</volume><issue>1</issue><spage>352</spage><epage>356</epage><pages>352-356</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>Recently D. D. Dunigan, R. G. Dietzgen, J. E. Schoelz, and M. Zaitlin (
Virology 165, 310–312,1988) demonstrated that a small proportion of the subunits of tobacco mosaic virus particles were conjugated with the small protein ubiquitin. We have now detected ubiquitinated conjugates in immunoblots of virion preparations of several other plant viruses, using anti-human ubiquitin antiserum. Based on their polyacrylamide gel migrations, plant virus-associated ubiquitin-immunoreactive proteins were considered to be possible virus structural protein-ubiquitin conjugates of the following viruses: barley stripe mosaic, brome mosaic, cowpea mosaic (two proteins), cowpea severe mosaic (two proteins), and satellite panicum mosaic. Ubiquitinated conjugates were not detected in immunoblots of preparations of cucumber mosaic virus and Cymbidium mosaic virus. The significance of ubiquitinated viral proteins remains to be determined.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2162109</pmid><doi>10.1016/0042-6822(90)90490-I</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1990-07, Vol.177 (1), p.352-356 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present) |
| subjects | AUTOGRAPHA AUTOGRAPHA CALIFORNICA Biological and medical sciences Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Immunoblotting INVERTEBRATE VIRUSES Microbiology Molecular Weight Morphology, structure, chemical composition, physicochemical properties Mosaic Viruses - analysis PLANT VIRUSES POLYHEDROSIS VIRUSES Protein Binding PROTEOLISIS PROTEOLYSE PROTEOLYSIS Species Specificity Ubiquitins - isolation & purification Viral Proteins - isolation & purification Virion - analysis Virology VIRUS DE INVERTEBRADOS VIRUS DE LAS PLANTAS VIRUS DES INVERTEBRES VIRUS DES VEGETAUX |
| title | Ubiquitinated conjugates are found in preparations of several plant viruses |
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