A lipopeptide-encoding sequence upstream from the lysA gene of Pseudomonas aeruginosa
An open reading frame (ORF) of 141 bp was observed upstream from the Pseudomonas aeruginosa lysA gene. The translation product of this ORF contains a signal peptide with a lipoprotein box, Ile-Ala-Ala-Cys, at the predicted signal peptidase cleavage site. The Escherichia coli phoA gene without its si...
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| Veröffentlicht in: | Molecular microbiology 1990-04, Vol.4 (4), p.677-682 |
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| creator | JANN, A CAVARD, D MARTIN, C CAMI, B PATTE, J.-C |
| description | An open reading frame (ORF) of 141 bp was observed upstream from the Pseudomonas aeruginosa lysA gene. The translation product of this ORF contains a signal peptide with a lipoprotein box, Ile-Ala-Ala-Cys, at the predicted signal peptidase cleavage site. The Escherichia coli phoA gene without its signal sequence was fused in frame to this ORF in a broad host-range plasmid. The resulting construct expressed a hybrid protein exhibiting alkaline phosphatase activity in phoA mutants of both E. coli and P. aeruginosa. This indicates that the ORF encodes a peptide, part of which acts as an export signal. The hybrid peptide was identified by immunoblotting with alkaline phosphatase antiserum. The accumulation of a precursor form was observed when P. aeruginosa cells carrying this gene fusion on a plasmid were treated with globomycin. Moreover, the mature form could be labelled with 2-[3H]-glycerol, indicating that lipidic residues may be linked to the hybrid protein. Taken together, these results strongly suggest that the ORF encodes a lipopeptide. We propose that the gene is called IppL. |
| doi_str_mv | 10.1111/j.1365-2958.1990.tb00637.x |
| format | Article |
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The translation product of this ORF contains a signal peptide with a lipoprotein box, Ile-Ala-Ala-Cys, at the predicted signal peptidase cleavage site. The Escherichia coli phoA gene without its signal sequence was fused in frame to this ORF in a broad host-range plasmid. The resulting construct expressed a hybrid protein exhibiting alkaline phosphatase activity in phoA mutants of both E. coli and P. aeruginosa. This indicates that the ORF encodes a peptide, part of which acts as an export signal. The hybrid peptide was identified by immunoblotting with alkaline phosphatase antiserum. The accumulation of a precursor form was observed when P. aeruginosa cells carrying this gene fusion on a plasmid were treated with globomycin. Moreover, the mature form could be labelled with 2-[3H]-glycerol, indicating that lipidic residues may be linked to the hybrid protein. Taken together, these results strongly suggest that the ORF encodes a lipopeptide. We propose that the gene is called IppL.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.1990.tb00637.x</identifier><identifier>PMID: 2112674</identifier><language>eng</language><publisher>Oxford: Blackwell Science</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biological and medical sciences ; Codon ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genes, Bacterial ; Genes. Genome ; Lipoproteins - biosynthesis ; Lipoproteins - genetics ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Protein Sorting Signals - genetics ; Pseudomonas aeruginosa - genetics ; Pseudomonas aeruginosa - growth & development ; Restriction Mapping ; RNA, Messenger</subject><ispartof>Molecular microbiology, 1990-04, Vol.4 (4), p.677-682</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6924693$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2112674$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JANN, A</creatorcontrib><creatorcontrib>CAVARD, D</creatorcontrib><creatorcontrib>MARTIN, C</creatorcontrib><creatorcontrib>CAMI, B</creatorcontrib><creatorcontrib>PATTE, J.-C</creatorcontrib><title>A lipopeptide-encoding sequence upstream from the lysA gene of Pseudomonas aeruginosa</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>An open reading frame (ORF) of 141 bp was observed upstream from the Pseudomonas aeruginosa lysA gene. The translation product of this ORF contains a signal peptide with a lipoprotein box, Ile-Ala-Ala-Cys, at the predicted signal peptidase cleavage site. The Escherichia coli phoA gene without its signal sequence was fused in frame to this ORF in a broad host-range plasmid. The resulting construct expressed a hybrid protein exhibiting alkaline phosphatase activity in phoA mutants of both E. coli and P. aeruginosa. This indicates that the ORF encodes a peptide, part of which acts as an export signal. The hybrid peptide was identified by immunoblotting with alkaline phosphatase antiserum. The accumulation of a precursor form was observed when P. aeruginosa cells carrying this gene fusion on a plasmid were treated with globomycin. Moreover, the mature form could be labelled with 2-[3H]-glycerol, indicating that lipidic residues may be linked to the hybrid protein. Taken together, these results strongly suggest that the ORF encodes a lipopeptide. We propose that the gene is called IppL.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Codon</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genes, Bacterial</subject><subject>Genes. Genome</subject><subject>Lipoproteins - biosynthesis</subject><subject>Lipoproteins - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Protein Sorting Signals - genetics</subject><subject>Pseudomonas aeruginosa - genetics</subject><subject>Pseudomonas aeruginosa - growth & development</subject><subject>Restriction Mapping</subject><subject>RNA, Messenger</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1Lw0AQBuBFlFqrP0FYRLwl7kezu3MspX5AQQ8WvIXdZFJTkmzMJmD_vQFDr85lGN6HYRhC7jiL-ViPh5hLlUQCEhNzABb3jjEldfxzRuan6JzMGSQskkZ8XpKrEA6McTm6GZkJzoXSyznZrWhVtr7Fti9zjLDJfF42exrwexgHpEMb-g5tTYvO17T_Qlodw4rusUHqC_oecMh97RsbqMVu2JeND_aaXBS2Cngz9QXZPW0-1i_R9u35db3aRi0H00eFyNkSCoMGUAOzuc64Mk5JhiBQZQg8kdop1IVmuTYGuJNOOFAjNU7IBXn429t2frw39Gldhgyryjboh5BqMEIJyf-FPEm0AqNHeDvBwdWYp21X1rY7ptPDxvx-ym3IbFV0tsnKcGIKxFKBlL_3tnzd</recordid><startdate>19900401</startdate><enddate>19900401</enddate><creator>JANN, A</creator><creator>CAVARD, D</creator><creator>MARTIN, C</creator><creator>CAMI, B</creator><creator>PATTE, J.-C</creator><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19900401</creationdate><title>A lipopeptide-encoding sequence upstream from the lysA gene of Pseudomonas aeruginosa</title><author>JANN, A ; CAVARD, D ; MARTIN, C ; CAMI, B ; PATTE, J.-C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p198t-f2d049f8e89e790ad7c168b630e92e6ce91537b6e7f70d78891b3b2b96ad78b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Codon</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genes, Bacterial</topic><topic>Genes. Genome</topic><topic>Lipoproteins - biosynthesis</topic><topic>Lipoproteins - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Protein Sorting Signals - genetics</topic><topic>Pseudomonas aeruginosa - genetics</topic><topic>Pseudomonas aeruginosa - growth & development</topic><topic>Restriction Mapping</topic><topic>RNA, Messenger</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JANN, A</creatorcontrib><creatorcontrib>CAVARD, D</creatorcontrib><creatorcontrib>MARTIN, C</creatorcontrib><creatorcontrib>CAMI, B</creatorcontrib><creatorcontrib>PATTE, J.-C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JANN, A</au><au>CAVARD, D</au><au>MARTIN, C</au><au>CAMI, B</au><au>PATTE, J.-C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A lipopeptide-encoding sequence upstream from the lysA gene of Pseudomonas aeruginosa</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>1990-04-01</date><risdate>1990</risdate><volume>4</volume><issue>4</issue><spage>677</spage><epage>682</epage><pages>677-682</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>An open reading frame (ORF) of 141 bp was observed upstream from the Pseudomonas aeruginosa lysA gene. The translation product of this ORF contains a signal peptide with a lipoprotein box, Ile-Ala-Ala-Cys, at the predicted signal peptidase cleavage site. The Escherichia coli phoA gene without its signal sequence was fused in frame to this ORF in a broad host-range plasmid. The resulting construct expressed a hybrid protein exhibiting alkaline phosphatase activity in phoA mutants of both E. coli and P. aeruginosa. This indicates that the ORF encodes a peptide, part of which acts as an export signal. The hybrid peptide was identified by immunoblotting with alkaline phosphatase antiserum. The accumulation of a precursor form was observed when P. aeruginosa cells carrying this gene fusion on a plasmid were treated with globomycin. Moreover, the mature form could be labelled with 2-[3H]-glycerol, indicating that lipidic residues may be linked to the hybrid protein. Taken together, these results strongly suggest that the ORF encodes a lipopeptide. We propose that the gene is called IppL.</abstract><cop>Oxford</cop><pub>Blackwell Science</pub><pmid>2112674</pmid><doi>10.1111/j.1365-2958.1990.tb00637.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | Molecular microbiology, 1990-04, Vol.4 (4), p.677-682 |
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| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences Codon Fundamental and applied biological sciences. Psychology Gene Expression Genes, Bacterial Genes. Genome Lipoproteins - biosynthesis Lipoproteins - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Protein Sorting Signals - genetics Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa - growth & development Restriction Mapping RNA, Messenger |
| title | A lipopeptide-encoding sequence upstream from the lysA gene of Pseudomonas aeruginosa |
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