Cellular and developmental properties of fetal hematopoietic stem cells
We have characterized the fetal totipotent hematopoietic stem cell using a novel strategy that integrates physical analysis of cell properties and genetic analysis of in vivo developmental behavior. This approach allows the simultaneous isolation and in vivo characterization of any stem cell populat...
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| Veröffentlicht in: | Cell 1990-06, Vol.61 (6), p.953-963 |
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| container_title | Cell |
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| creator | Jordan, Craig T. McKearn, John P. Lemischka, Ihor R. |
| description | We have characterized the fetal totipotent hematopoietic stem cell using a novel strategy that integrates physical analysis of cell properties and genetic analysis of in vivo developmental behavior. This approach allows the simultaneous isolation and in vivo characterization of any stem cell population. Using this procedure we demonstrate that a cell surface marker, recognized by monoclonal antibody AA4.1, defines 0.5%–1.0% of fetal liver tissue that contains the entire hierarchy of primitive hematopoietic cells. The AA4.1
+ subpopulation includes multipotential in vitro progenitors, CFU-S cells, and lymphoid-myeloid stem cells that function to yield permanent and oligoclonal blood systems. Further fractionation of these cells by analysis of density, fibronectin binding, and surface antigen distribution has defined 0.1%–0.2% of fetal liver that contains the totipotent stem cell. |
| doi_str_mv | 10.1016/0092-8674(90)90061-I |
| format | Article |
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+ subpopulation includes multipotential in vitro progenitors, CFU-S cells, and lymphoid-myeloid stem cells that function to yield permanent and oligoclonal blood systems. Further fractionation of these cells by analysis of density, fibronectin binding, and surface antigen distribution has defined 0.1%–0.2% of fetal liver that contains the totipotent stem cell.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(90)90061-I</identifier><identifier>PMID: 1972037</identifier><identifier>CODEN: CELLB5</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Animals ; Antibodies, Monoclonal - analysis ; Antigens, Surface - analysis ; Biological and medical sciences ; Blotting, Southern ; Bone Marrow Cells ; Cell Adhesion ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; Cell Separation ; Cells, Cultured ; Colony-Forming Units Assay ; Female ; Fibronectins - metabolism ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells - cytology ; Liver - cytology ; Liver - embryology ; Mice ; Mice, Inbred C3H ; Molecular and cellular biology ; Pregnancy ; Receptors, Fibronectin ; Receptors, Immunologic - analysis ; Spleen - cytology</subject><ispartof>Cell, 1990-06, Vol.61 (6), p.953-963</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-a3649fa2e79f1e252608162bcf24f3b74e9f2fdb18517c903dc3fd383f48fae3</citedby><cites>FETCH-LOGICAL-c387t-a3649fa2e79f1e252608162bcf24f3b74e9f2fdb18517c903dc3fd383f48fae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0092-8674(90)90061-I$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19304799$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1972037$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jordan, Craig T.</creatorcontrib><creatorcontrib>McKearn, John P.</creatorcontrib><creatorcontrib>Lemischka, Ihor R.</creatorcontrib><title>Cellular and developmental properties of fetal hematopoietic stem cells</title><title>Cell</title><addtitle>Cell</addtitle><description>We have characterized the fetal totipotent hematopoietic stem cell using a novel strategy that integrates physical analysis of cell properties and genetic analysis of in vivo developmental behavior. This approach allows the simultaneous isolation and in vivo characterization of any stem cell population. Using this procedure we demonstrate that a cell surface marker, recognized by monoclonal antibody AA4.1, defines 0.5%–1.0% of fetal liver tissue that contains the entire hierarchy of primitive hematopoietic cells. The AA4.1
+ subpopulation includes multipotential in vitro progenitors, CFU-S cells, and lymphoid-myeloid stem cells that function to yield permanent and oligoclonal blood systems. Further fractionation of these cells by analysis of density, fibronectin binding, and surface antigen distribution has defined 0.1%–0.2% of fetal liver that contains the totipotent stem cell.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - analysis</subject><subject>Antigens, Surface - analysis</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Bone Marrow Cells</subject><subject>Cell Adhesion</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>Cell Separation</subject><subject>Cells, Cultured</subject><subject>Colony-Forming Units Assay</subject><subject>Female</subject><subject>Fibronectins - metabolism</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hematopoietic Stem Cell Transplantation</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Liver - cytology</subject><subject>Liver - embryology</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Molecular and cellular biology</subject><subject>Pregnancy</subject><subject>Receptors, Fibronectin</subject><subject>Receptors, Immunologic - analysis</subject><subject>Spleen - cytology</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKBDEQRYMoOo7-gUJvFF20Vh7dSTaCDD4GBDfuQyZdwUi_THoE_95uZ1BXrgKpU5dbh5ATClcUaHkNoFmuSikuNFxqgJLmyx0yo6BlLqhku2T2gxyQw5TeAEAVRbFP9qmWDLickYcF1vW6tjGzbZVV-IF11zfYDrbO-tj1GIeAKet85nH6e8XGDl3fBRyCy9KATebGhHRE9rytEx5v3zl5ub97WTzmT88Py8XtU-64kkNueSm0twyl9hRZwUpQtGQr55nwfCUFas98taKqoNJp4JXjvuKKe6G8RT4n55vYsdv7GtNgmpCmArbFbp2M1IoJNZ42J2IDutilFNGbPobGxk9DwUz6zOTGTG6MBvOtzyzHtdNt_nrVYPW7tPE1zs-2c5ucrX20rQvpD8ZBSK1H7mbD4ejiI2A0yQVsHVYhohtM1YX_i3wBmA6MYg</recordid><startdate>19900615</startdate><enddate>19900615</enddate><creator>Jordan, Craig T.</creator><creator>McKearn, John P.</creator><creator>Lemischka, Ihor R.</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900615</creationdate><title>Cellular and developmental properties of fetal hematopoietic stem cells</title><author>Jordan, Craig T. ; McKearn, John P. ; Lemischka, Ihor R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-a3649fa2e79f1e252608162bcf24f3b74e9f2fdb18517c903dc3fd383f48fae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - analysis</topic><topic>Antigens, Surface - analysis</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Bone Marrow Cells</topic><topic>Cell Adhesion</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>Cell Separation</topic><topic>Cells, Cultured</topic><topic>Colony-Forming Units Assay</topic><topic>Female</topic><topic>Fibronectins - metabolism</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hematopoietic Stem Cell Transplantation</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Liver - cytology</topic><topic>Liver - embryology</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Molecular and cellular biology</topic><topic>Pregnancy</topic><topic>Receptors, Fibronectin</topic><topic>Receptors, Immunologic - analysis</topic><topic>Spleen - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jordan, Craig T.</creatorcontrib><creatorcontrib>McKearn, John P.</creatorcontrib><creatorcontrib>Lemischka, Ihor R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jordan, Craig T.</au><au>McKearn, John P.</au><au>Lemischka, Ihor R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellular and developmental properties of fetal hematopoietic stem cells</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1990-06-15</date><risdate>1990</risdate><volume>61</volume><issue>6</issue><spage>953</spage><epage>963</epage><pages>953-963</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><coden>CELLB5</coden><abstract>We have characterized the fetal totipotent hematopoietic stem cell using a novel strategy that integrates physical analysis of cell properties and genetic analysis of in vivo developmental behavior. This approach allows the simultaneous isolation and in vivo characterization of any stem cell population. Using this procedure we demonstrate that a cell surface marker, recognized by monoclonal antibody AA4.1, defines 0.5%–1.0% of fetal liver tissue that contains the entire hierarchy of primitive hematopoietic cells. The AA4.1
+ subpopulation includes multipotential in vitro progenitors, CFU-S cells, and lymphoid-myeloid stem cells that function to yield permanent and oligoclonal blood systems. Further fractionation of these cells by analysis of density, fibronectin binding, and surface antigen distribution has defined 0.1%–0.2% of fetal liver that contains the totipotent stem cell.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>1972037</pmid><doi>10.1016/0092-8674(90)90061-I</doi><tpages>11</tpages></addata></record> |
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| ispartof | Cell, 1990-06, Vol.61 (6), p.953-963 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Antibodies, Monoclonal - analysis Antigens, Surface - analysis Biological and medical sciences Blotting, Southern Bone Marrow Cells Cell Adhesion Cell differentiation, maturation, development, hematopoiesis Cell physiology Cell Separation Cells, Cultured Colony-Forming Units Assay Female Fibronectins - metabolism Flow Cytometry Fundamental and applied biological sciences. Psychology Hematopoietic Stem Cell Transplantation Hematopoietic Stem Cells - cytology Liver - cytology Liver - embryology Mice Mice, Inbred C3H Molecular and cellular biology Pregnancy Receptors, Fibronectin Receptors, Immunologic - analysis Spleen - cytology |
| title | Cellular and developmental properties of fetal hematopoietic stem cells |
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