Arecoline stimulation of radiolabeled arachidonate incorporation from plasma into brain microvessels of awake rat
The cholinergic agonist, arecoline, was used to examine the effects of cholinergic stimulation upon incorporation of radiolabeled arachidonic acid from blood into cerebral microvessels of awake rats. Animals received a single i.p. injection of arecoline (1 mg/kg) followed 3 to 5 minutes later by a 5...
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| Veröffentlicht in: | Neurochemical research 1998-04, Vol.23 (4), p.551-555 |
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| creator | WILLIAMS, W. M HAYAKAWA, T GRANGE, E RAPOPORT, S. I |
| description | The cholinergic agonist, arecoline, was used to examine the effects of cholinergic stimulation upon incorporation of radiolabeled arachidonic acid from blood into cerebral microvessels of awake rats. Animals received a single i.p. injection of arecoline (1 mg/kg) followed 3 to 5 minutes later by a 5 minute intravenous infusion of [1-14C]arachidonic acid (AA) (170 microCi/kg) via the femoral vein. Timed arterial blood samples were collected over 20 minutes following the start of infusion, after which the animal was killed, and the brain was removed. The incorporation coefficient k* for [1-14C]AA was approximately 2-fold higher in microvessels isolated from arecoline-injected than from sham-injected animals. The data demonstrate in an in vivo paradigm, that activation of cholinergic pathways within the rat CNS stimulates arachidonic acid turnover in cerebral microvessels. This suggests a direct involvement of this fatty acid in second messenger function within microvessel endothelial cells and possibly attached pericytes. |
| doi_str_mv | 10.1023/A:1022490820109 |
| format | Article |
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M ; HAYAKAWA, T ; GRANGE, E ; RAPOPORT, S. I</creator><creatorcontrib>WILLIAMS, W. M ; HAYAKAWA, T ; GRANGE, E ; RAPOPORT, S. I</creatorcontrib><description>The cholinergic agonist, arecoline, was used to examine the effects of cholinergic stimulation upon incorporation of radiolabeled arachidonic acid from blood into cerebral microvessels of awake rats. Animals received a single i.p. injection of arecoline (1 mg/kg) followed 3 to 5 minutes later by a 5 minute intravenous infusion of [1-14C]arachidonic acid (AA) (170 microCi/kg) via the femoral vein. Timed arterial blood samples were collected over 20 minutes following the start of infusion, after which the animal was killed, and the brain was removed. The incorporation coefficient k* for [1-14C]AA was approximately 2-fold higher in microvessels isolated from arecoline-injected than from sham-injected animals. The data demonstrate in an in vivo paradigm, that activation of cholinergic pathways within the rat CNS stimulates arachidonic acid turnover in cerebral microvessels. This suggests a direct involvement of this fatty acid in second messenger function within microvessel endothelial cells and possibly attached pericytes.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1023/A:1022490820109</identifier><identifier>PMID: 9566591</identifier><identifier>CODEN: NEREDZ</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Animals ; Arachidonic Acid - blood ; Arachidonic Acid - metabolism ; Arecoline - pharmacology ; Biological and medical sciences ; Blood-Brain Barrier - drug effects ; Blood-Brain Barrier - physiology ; Brain - blood supply ; Brain - metabolism ; Carbon Radioisotopes ; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges ; Cerebrovascular Circulation - drug effects ; Cerebrovascular Circulation - physiology ; Fatty acids ; Fundamental and applied biological sciences. Psychology ; Incorporation ; Kinetics ; Male ; Microcirculation - drug effects ; Microcirculation - physiology ; Plasma ; Radioisotope Dilution Technique ; Rats ; Rats, Inbred F344 ; Rodents ; Vertebrates: nervous system and sense organs ; Wakefulness</subject><ispartof>Neurochemical research, 1998-04, Vol.23 (4), p.551-555</ispartof><rights>1998 INIST-CNRS</rights><rights>Plenum Publishing Corporation 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2216995$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9566591$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WILLIAMS, W. M</creatorcontrib><creatorcontrib>HAYAKAWA, T</creatorcontrib><creatorcontrib>GRANGE, E</creatorcontrib><creatorcontrib>RAPOPORT, S. I</creatorcontrib><title>Arecoline stimulation of radiolabeled arachidonate incorporation from plasma into brain microvessels of awake rat</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><description>The cholinergic agonist, arecoline, was used to examine the effects of cholinergic stimulation upon incorporation of radiolabeled arachidonic acid from blood into cerebral microvessels of awake rats. Animals received a single i.p. injection of arecoline (1 mg/kg) followed 3 to 5 minutes later by a 5 minute intravenous infusion of [1-14C]arachidonic acid (AA) (170 microCi/kg) via the femoral vein. Timed arterial blood samples were collected over 20 minutes following the start of infusion, after which the animal was killed, and the brain was removed. The incorporation coefficient k* for [1-14C]AA was approximately 2-fold higher in microvessels isolated from arecoline-injected than from sham-injected animals. The data demonstrate in an in vivo paradigm, that activation of cholinergic pathways within the rat CNS stimulates arachidonic acid turnover in cerebral microvessels. This suggests a direct involvement of this fatty acid in second messenger function within microvessel endothelial cells and possibly attached pericytes.</description><subject>Animals</subject><subject>Arachidonic Acid - blood</subject><subject>Arachidonic Acid - metabolism</subject><subject>Arecoline - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Blood-Brain Barrier - drug effects</subject><subject>Blood-Brain Barrier - physiology</subject><subject>Brain - blood supply</subject><subject>Brain - metabolism</subject><subject>Carbon Radioisotopes</subject><subject>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</subject><subject>Cerebrovascular Circulation - drug effects</subject><subject>Cerebrovascular Circulation - physiology</subject><subject>Fatty acids</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Incorporation</subject><subject>Kinetics</subject><subject>Male</subject><subject>Microcirculation - drug effects</subject><subject>Microcirculation - physiology</subject><subject>Plasma</subject><subject>Radioisotope Dilution Technique</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Rodents</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Wakefulness</subject><issn>0364-3190</issn><issn>1573-6903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkD1LxTAYhYMoev2YnYSC4lZ989G0cbuIXyC46Fzem6QYTZuatIr_3ogXBxenM5yHB84h5JDCGQXGz5cXOZhQ0DCgoDbIglY1L6UCvkkWwKUoOVWwQ3ZTeoGMAKPbZFtVUlaKLsjbMlodvBtskSbXzx4nF4YidEVE44LHlfXWFBhRPzsTBpxs4QYd4hjiD9rF0Bejx9RjbqZQrCK6oeidjuHdpmR9-tbhB77aLJ32yVaHPtmDde6Rp-urx8vb8v7h5u5yeV-OTImp5CAMtaxDKWsGgnLdGGgUGFl3nBvO87ZO1ZqvmFYIKi8Vuma1qbTQojN8j5z-eMcY3mabprZ3SVvvcbBhTm2tGibyhf-CVPKKSYAMHv8BX8IchzyizdeLGnjTyEwdral51VvTjtH1GD_b9eW5P1n3mDT6LuKgXfrFGKNSqYp_AbFDj80</recordid><startdate>19980401</startdate><enddate>19980401</enddate><creator>WILLIAMS, W. 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M ; HAYAKAWA, T ; GRANGE, E ; RAPOPORT, S. I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p294t-304d1e2fa66720413c8d0890d67f33d33036f97c3b2c9a093644c727d5c4c4fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Arachidonic Acid - blood</topic><topic>Arachidonic Acid - metabolism</topic><topic>Arecoline - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Blood-Brain Barrier - drug effects</topic><topic>Blood-Brain Barrier - physiology</topic><topic>Brain - blood supply</topic><topic>Brain - metabolism</topic><topic>Carbon Radioisotopes</topic><topic>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</topic><topic>Cerebrovascular Circulation - drug effects</topic><topic>Cerebrovascular Circulation - physiology</topic><topic>Fatty acids</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Incorporation</topic><topic>Kinetics</topic><topic>Male</topic><topic>Microcirculation - drug effects</topic><topic>Microcirculation - physiology</topic><topic>Plasma</topic><topic>Radioisotope Dilution Technique</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Rodents</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Wakefulness</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WILLIAMS, W. M</creatorcontrib><creatorcontrib>HAYAKAWA, T</creatorcontrib><creatorcontrib>GRANGE, E</creatorcontrib><creatorcontrib>RAPOPORT, S. 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M</au><au>HAYAKAWA, T</au><au>GRANGE, E</au><au>RAPOPORT, S. I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arecoline stimulation of radiolabeled arachidonate incorporation from plasma into brain microvessels of awake rat</atitle><jtitle>Neurochemical research</jtitle><addtitle>Neurochem Res</addtitle><date>1998-04-01</date><risdate>1998</risdate><volume>23</volume><issue>4</issue><spage>551</spage><epage>555</epage><pages>551-555</pages><issn>0364-3190</issn><eissn>1573-6903</eissn><coden>NEREDZ</coden><abstract>The cholinergic agonist, arecoline, was used to examine the effects of cholinergic stimulation upon incorporation of radiolabeled arachidonic acid from blood into cerebral microvessels of awake rats. Animals received a single i.p. injection of arecoline (1 mg/kg) followed 3 to 5 minutes later by a 5 minute intravenous infusion of [1-14C]arachidonic acid (AA) (170 microCi/kg) via the femoral vein. Timed arterial blood samples were collected over 20 minutes following the start of infusion, after which the animal was killed, and the brain was removed. The incorporation coefficient k* for [1-14C]AA was approximately 2-fold higher in microvessels isolated from arecoline-injected than from sham-injected animals. The data demonstrate in an in vivo paradigm, that activation of cholinergic pathways within the rat CNS stimulates arachidonic acid turnover in cerebral microvessels. This suggests a direct involvement of this fatty acid in second messenger function within microvessel endothelial cells and possibly attached pericytes.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>9566591</pmid><doi>10.1023/A:1022490820109</doi><tpages>5</tpages></addata></record> |
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| ispartof | Neurochemical research, 1998-04, Vol.23 (4), p.551-555 |
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| subjects | Animals Arachidonic Acid - blood Arachidonic Acid - metabolism Arecoline - pharmacology Biological and medical sciences Blood-Brain Barrier - drug effects Blood-Brain Barrier - physiology Brain - blood supply Brain - metabolism Carbon Radioisotopes Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges Cerebrovascular Circulation - drug effects Cerebrovascular Circulation - physiology Fatty acids Fundamental and applied biological sciences. Psychology Incorporation Kinetics Male Microcirculation - drug effects Microcirculation - physiology Plasma Radioisotope Dilution Technique Rats Rats, Inbred F344 Rodents Vertebrates: nervous system and sense organs Wakefulness |
| title | Arecoline stimulation of radiolabeled arachidonate incorporation from plasma into brain microvessels of awake rat |
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