Rotavirus infection of cultured intestinal epithelial cells induces secretion of CXC and CC chemokines
Background & Aims: Rotaviruses are the major cause of pediatric gastroenteritis worldwide. The target cell of rotavirus infection is the mature enterocyte of the small intestine. Recently, intestinal epithelial cells have been shown to produce chemoattractant mediators in response to cytokine st...
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| Veröffentlicht in: | Gastroenterology (New York, N.Y. 1943) N.Y. 1943), 1998-05, Vol.114 (5), p.947-955 |
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| container_title | Gastroenterology (New York, N.Y. 1943) |
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| creator | Casola, Antonella Estes ‡, Mary K. Crawford ‡, Sue E. Ogra, Pearay L. Ernst, Peter B. Garofalo, Roberto P. Crowe §, Sheila E. |
| description | Background & Aims: Rotaviruses are the major cause of pediatric gastroenteritis worldwide. The target cell of rotavirus infection is the mature enterocyte of the small intestine. Recently, intestinal epithelial cells have been shown to produce chemoattractant mediators in response to cytokine stimulation and bacterial infection, suggesting a potentially important role of epithelial cells in initiating immune responses. In this study, the production of chemokines by cultured intestinal epithelial cells after rotavirus infection was investigated.
Methods: Two human intestinal epithelial cell lines (HT29 and Caco-2) were infected with sucrose-purified rotavirus (strain SA114F) and assayed by reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay for chemokine expression. Virus-like particles and inactivated rotavirus were used to test the importance of viral attachment and replication.
Results: Increased messenger RNA expression and secretion of immunoreactive interleukin 8, growth-related peptide α, and RANTES (regulated upon activation, normal T cell expressed and secreted) were detected in rotavirus-infected cells. Chemokine production was time and dose dependent and required viral replication.
Conclusions: Rotavirus infection induces the expression of a subset of chemokines in intestinal epithelial cells. These data support the hypothesis that chemokine secretion by enterocytes may play a role in the initiation and modulation of the immune response to rotavirus infection.
GASTROENTEROLOGY 1998;114:947-955 |
| doi_str_mv | 10.1016/S0016-5085(98)70314-2 |
| format | Article |
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Methods: Two human intestinal epithelial cell lines (HT29 and Caco-2) were infected with sucrose-purified rotavirus (strain SA114F) and assayed by reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay for chemokine expression. Virus-like particles and inactivated rotavirus were used to test the importance of viral attachment and replication.
Results: Increased messenger RNA expression and secretion of immunoreactive interleukin 8, growth-related peptide α, and RANTES (regulated upon activation, normal T cell expressed and secreted) were detected in rotavirus-infected cells. Chemokine production was time and dose dependent and required viral replication.
Conclusions: Rotavirus infection induces the expression of a subset of chemokines in intestinal epithelial cells. These data support the hypothesis that chemokine secretion by enterocytes may play a role in the initiation and modulation of the immune response to rotavirus infection.
GASTROENTEROLOGY 1998;114:947-955</description><identifier>ISSN: 0016-5085</identifier><identifier>EISSN: 1528-0012</identifier><identifier>DOI: 10.1016/S0016-5085(98)70314-2</identifier><identifier>PMID: 9558283</identifier><identifier>CODEN: GASTAB</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Biological and medical sciences ; Chemokine CCL5 - genetics ; Chemokine CXCL1 ; Chemokines, CC - metabolism ; Chemokines, CXC - metabolism ; Chemotactic Factors - genetics ; Growth Inhibitors - genetics ; Growth Substances - genetics ; Human viral diseases ; Humans ; Infectious diseases ; Intercellular Signaling Peptides and Proteins ; Interleukin-8 - genetics ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - pathology ; Medical sciences ; RNA, Messenger - metabolism ; Rotavirus Infections - metabolism ; Rotavirus Infections - virology ; Time Factors ; Viral diseases ; Viral diseases of the digestive system ; Viral Proteins - pharmacology ; Virus Replication - physiology</subject><ispartof>Gastroenterology (New York, N.Y. 1943), 1998-05, Vol.114 (5), p.947-955</ispartof><rights>1998 American Gastroenterological Association</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-f84593cd29dcbd07fbbfff13f5381b2448852b81a9c68d092c178e47b3963fe93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0016-5085(98)70314-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2220740$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9558283$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Casola, Antonella</creatorcontrib><creatorcontrib>Estes ‡, Mary K.</creatorcontrib><creatorcontrib>Crawford ‡, Sue E.</creatorcontrib><creatorcontrib>Ogra, Pearay L.</creatorcontrib><creatorcontrib>Ernst, Peter B.</creatorcontrib><creatorcontrib>Garofalo, Roberto P.</creatorcontrib><creatorcontrib>Crowe §, Sheila E.</creatorcontrib><title>Rotavirus infection of cultured intestinal epithelial cells induces secretion of CXC and CC chemokines</title><title>Gastroenterology (New York, N.Y. 1943)</title><addtitle>Gastroenterology</addtitle><description>Background & Aims: Rotaviruses are the major cause of pediatric gastroenteritis worldwide. The target cell of rotavirus infection is the mature enterocyte of the small intestine. Recently, intestinal epithelial cells have been shown to produce chemoattractant mediators in response to cytokine stimulation and bacterial infection, suggesting a potentially important role of epithelial cells in initiating immune responses. In this study, the production of chemokines by cultured intestinal epithelial cells after rotavirus infection was investigated.
Methods: Two human intestinal epithelial cell lines (HT29 and Caco-2) were infected with sucrose-purified rotavirus (strain SA114F) and assayed by reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay for chemokine expression. Virus-like particles and inactivated rotavirus were used to test the importance of viral attachment and replication.
Results: Increased messenger RNA expression and secretion of immunoreactive interleukin 8, growth-related peptide α, and RANTES (regulated upon activation, normal T cell expressed and secreted) were detected in rotavirus-infected cells. Chemokine production was time and dose dependent and required viral replication.
Conclusions: Rotavirus infection induces the expression of a subset of chemokines in intestinal epithelial cells. These data support the hypothesis that chemokine secretion by enterocytes may play a role in the initiation and modulation of the immune response to rotavirus infection.
GASTROENTEROLOGY 1998;114:947-955</description><subject>Biological and medical sciences</subject><subject>Chemokine CCL5 - genetics</subject><subject>Chemokine CXCL1</subject><subject>Chemokines, CC - metabolism</subject><subject>Chemokines, CXC - metabolism</subject><subject>Chemotactic Factors - genetics</subject><subject>Growth Inhibitors - genetics</subject><subject>Growth Substances - genetics</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Intercellular Signaling Peptides and Proteins</subject><subject>Interleukin-8 - genetics</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - pathology</subject><subject>Medical sciences</subject><subject>RNA, Messenger - metabolism</subject><subject>Rotavirus Infections - metabolism</subject><subject>Rotavirus Infections - virology</subject><subject>Time Factors</subject><subject>Viral diseases</subject><subject>Viral diseases of the digestive system</subject><subject>Viral Proteins - pharmacology</subject><subject>Virus Replication - physiology</subject><issn>0016-5085</issn><issn>1528-0012</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtLxDAUhYMoOj5-gtCFiC6qeTTTZCVSfMGA4APchTS5wWinHZN2wH9v6oyzdZOEnHNuDl8QOib4gmAyvXzGac05FvxMivMSM1LkdAtNCKciTxrdRpONZQ_tx_iBMZZMkF20KzkXVLAJck9dr5c-DDHzrQPT-67NOpeZoemHADbd9hB73-omg4Xv36Hx6WigacaEHQzELIIJ8Jes3qpMtzarqsy8w7z79C3EQ7TjdBPhaL0foNfbm5fqPp893j1U17PcsBL3uRMFl8xYKq2pLS5dXTvnCHM81a5pUQjBaS2IlmYqLJbUkFJAUdZMTpkDyQ7Q6WruInRfQyqu5j6OZXUL3RBVKQVllItk5CujCV2MAZxaBD_X4VsRrEa-6pevGuEpKdQvX0VT7nj9wFDPwW5Sa6BJP1nrOhrduKBb4-PGRinFZYGT7WplgwRj6SGoaDy0BqwP6ROU7fw_RX4APTOX2g</recordid><startdate>199805</startdate><enddate>199805</enddate><creator>Casola, Antonella</creator><creator>Estes ‡, Mary K.</creator><creator>Crawford ‡, Sue E.</creator><creator>Ogra, Pearay L.</creator><creator>Ernst, Peter B.</creator><creator>Garofalo, Roberto P.</creator><creator>Crowe §, Sheila E.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199805</creationdate><title>Rotavirus infection of cultured intestinal epithelial cells induces secretion of CXC and CC chemokines</title><author>Casola, Antonella ; Estes ‡, Mary K. ; Crawford ‡, Sue E. ; Ogra, Pearay L. ; Ernst, Peter B. ; Garofalo, Roberto P. ; Crowe §, Sheila E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-f84593cd29dcbd07fbbfff13f5381b2448852b81a9c68d092c178e47b3963fe93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological and medical sciences</topic><topic>Chemokine CCL5 - genetics</topic><topic>Chemokine CXCL1</topic><topic>Chemokines, CC - metabolism</topic><topic>Chemokines, CXC - metabolism</topic><topic>Chemotactic Factors - genetics</topic><topic>Growth Inhibitors - genetics</topic><topic>Growth Substances - genetics</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Intercellular Signaling Peptides and Proteins</topic><topic>Interleukin-8 - genetics</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestinal Mucosa - pathology</topic><topic>Medical sciences</topic><topic>RNA, Messenger - metabolism</topic><topic>Rotavirus Infections - metabolism</topic><topic>Rotavirus Infections - virology</topic><topic>Time Factors</topic><topic>Viral diseases</topic><topic>Viral diseases of the digestive system</topic><topic>Viral Proteins - pharmacology</topic><topic>Virus Replication - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Casola, Antonella</creatorcontrib><creatorcontrib>Estes ‡, Mary K.</creatorcontrib><creatorcontrib>Crawford ‡, Sue E.</creatorcontrib><creatorcontrib>Ogra, Pearay L.</creatorcontrib><creatorcontrib>Ernst, Peter B.</creatorcontrib><creatorcontrib>Garofalo, Roberto P.</creatorcontrib><creatorcontrib>Crowe §, Sheila E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Casola, Antonella</au><au>Estes ‡, Mary K.</au><au>Crawford ‡, Sue E.</au><au>Ogra, Pearay L.</au><au>Ernst, Peter B.</au><au>Garofalo, Roberto P.</au><au>Crowe §, Sheila E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rotavirus infection of cultured intestinal epithelial cells induces secretion of CXC and CC chemokines</atitle><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle><addtitle>Gastroenterology</addtitle><date>1998-05</date><risdate>1998</risdate><volume>114</volume><issue>5</issue><spage>947</spage><epage>955</epage><pages>947-955</pages><issn>0016-5085</issn><eissn>1528-0012</eissn><coden>GASTAB</coden><abstract>Background & Aims: Rotaviruses are the major cause of pediatric gastroenteritis worldwide. The target cell of rotavirus infection is the mature enterocyte of the small intestine. Recently, intestinal epithelial cells have been shown to produce chemoattractant mediators in response to cytokine stimulation and bacterial infection, suggesting a potentially important role of epithelial cells in initiating immune responses. In this study, the production of chemokines by cultured intestinal epithelial cells after rotavirus infection was investigated.
Methods: Two human intestinal epithelial cell lines (HT29 and Caco-2) were infected with sucrose-purified rotavirus (strain SA114F) and assayed by reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay for chemokine expression. Virus-like particles and inactivated rotavirus were used to test the importance of viral attachment and replication.
Results: Increased messenger RNA expression and secretion of immunoreactive interleukin 8, growth-related peptide α, and RANTES (regulated upon activation, normal T cell expressed and secreted) were detected in rotavirus-infected cells. Chemokine production was time and dose dependent and required viral replication.
Conclusions: Rotavirus infection induces the expression of a subset of chemokines in intestinal epithelial cells. These data support the hypothesis that chemokine secretion by enterocytes may play a role in the initiation and modulation of the immune response to rotavirus infection.
GASTROENTEROLOGY 1998;114:947-955</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>9558283</pmid><doi>10.1016/S0016-5085(98)70314-2</doi><tpages>9</tpages></addata></record> |
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| subjects | Biological and medical sciences Chemokine CCL5 - genetics Chemokine CXCL1 Chemokines, CC - metabolism Chemokines, CXC - metabolism Chemotactic Factors - genetics Growth Inhibitors - genetics Growth Substances - genetics Human viral diseases Humans Infectious diseases Intercellular Signaling Peptides and Proteins Interleukin-8 - genetics Intestinal Mucosa - metabolism Intestinal Mucosa - pathology Medical sciences RNA, Messenger - metabolism Rotavirus Infections - metabolism Rotavirus Infections - virology Time Factors Viral diseases Viral diseases of the digestive system Viral Proteins - pharmacology Virus Replication - physiology |
| title | Rotavirus infection of cultured intestinal epithelial cells induces secretion of CXC and CC chemokines |
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