ATP and ADP in human pre-embryos
Spare human oocytes and pre-embryos from an in-vitro fertilization (IVF) programme were individually analysed for ATP and ADP content using a bioluminescence method employing the firefly luceferin-luciferase reaction. The ATPcontent of oocytes that failed to fertilize in vitro was 1.71 ⊞ 0.28 pmol (...
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| Veröffentlicht in: | Human reproduction (Oxford) 1990-04, Vol.5 (3), p.319-322 |
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| creator | Slotte, Håkan Gustafson, Ove Nylund, Lars Pousette, Åke |
| description | Spare human oocytes and pre-embryos from an in-vitro fertilization (IVF) programme were individually analysed for ATP and ADP content using a bioluminescence method employing the firefly luceferin-luciferase reaction. The ATPcontent of oocytes that failed to fertilize in vitro was 1.71 ⊞ 0.28 pmol (n = 10), pronuclear stage ova1.93 ± 0.08 (n = 6), 2-cell stage 1.78 ± 0.20 (n = 7), 4-cell stage 1.73 ± 0.15 (n = 6), 6–8 cell stage 2.76 ± 0.53 (n = 8), morula stage 2.36 ± 0.68 (n = 8), early blastocyst stage 2.08 ± 0.25 (n = 7) and expanded blastocyst stage 2.26 ± 0.15 (n = 3) pmol. The ADP content of these pre-embryos was low in all stages with a small increment in the 2–4 cell stages and the blastocyst stage. This gave an elevated ATP/ADP ratio (range 19–92) indicating a goodenergy status. The sensitive luciferin-luciferase assay may be a tool for studying the energy status of spare oocytes and pre-embryos under different incubation conditions in human IVF programmes. |
| doi_str_mv | 10.1093/oxfordjournals.humrep.a137097 |
| format | Article |
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The ATPcontent of oocytes that failed to fertilize in vitro was 1.71 ⊞ 0.28 pmol (n = 10), pronuclear stage ova1.93 ± 0.08 (n = 6), 2-cell stage 1.78 ± 0.20 (n = 7), 4-cell stage 1.73 ± 0.15 (n = 6), 6–8 cell stage 2.76 ± 0.53 (n = 8), morula stage 2.36 ± 0.68 (n = 8), early blastocyst stage 2.08 ± 0.25 (n = 7) and expanded blastocyst stage 2.26 ± 0.15 (n = 3) pmol. The ADP content of these pre-embryos was low in all stages with a small increment in the 2–4 cell stages and the blastocyst stage. This gave an elevated ATP/ADP ratio (range 19–92) indicating a goodenergy status. The sensitive luciferin-luciferase assay may be a tool for studying the energy status of spare oocytes and pre-embryos under different incubation conditions in human IVF programmes.</description><identifier>ISSN: 0268-1161</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/oxfordjournals.humrep.a137097</identifier><identifier>PMID: 2351715</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adenosine Diphosphate - analysis ; Adenosine Monophosphate - analysis ; ADP ; ATP ; bioluminescence ; Blastocyst - analysis ; embryp ; Humans ; Morula - analysis ; oocyte ; Oocytes - analysis ; Zygote - analysis</subject><ispartof>Human reproduction (Oxford), 1990-04, Vol.5 (3), p.319-322</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c273t-9be573bcb7c0143f9c8394eec47dfee0abc48f3d3974c826855abda6415381fa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2351715$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Slotte, Håkan</creatorcontrib><creatorcontrib>Gustafson, Ove</creatorcontrib><creatorcontrib>Nylund, Lars</creatorcontrib><creatorcontrib>Pousette, Åke</creatorcontrib><title>ATP and ADP in human pre-embryos</title><title>Human reproduction (Oxford)</title><addtitle>Hum Reprod</addtitle><description>Spare human oocytes and pre-embryos from an in-vitro fertilization (IVF) programme were individually analysed for ATP and ADP content using a bioluminescence method employing the firefly luceferin-luciferase reaction. The ATPcontent of oocytes that failed to fertilize in vitro was 1.71 ⊞ 0.28 pmol (n = 10), pronuclear stage ova1.93 ± 0.08 (n = 6), 2-cell stage 1.78 ± 0.20 (n = 7), 4-cell stage 1.73 ± 0.15 (n = 6), 6–8 cell stage 2.76 ± 0.53 (n = 8), morula stage 2.36 ± 0.68 (n = 8), early blastocyst stage 2.08 ± 0.25 (n = 7) and expanded blastocyst stage 2.26 ± 0.15 (n = 3) pmol. The ADP content of these pre-embryos was low in all stages with a small increment in the 2–4 cell stages and the blastocyst stage. This gave an elevated ATP/ADP ratio (range 19–92) indicating a goodenergy status. The sensitive luciferin-luciferase assay may be a tool for studying the energy status of spare oocytes and pre-embryos under different incubation conditions in human IVF programmes.</description><subject>Adenosine Diphosphate - analysis</subject><subject>Adenosine Monophosphate - analysis</subject><subject>ADP</subject><subject>ATP</subject><subject>bioluminescence</subject><subject>Blastocyst - analysis</subject><subject>embryp</subject><subject>Humans</subject><subject>Morula - analysis</subject><subject>oocyte</subject><subject>Oocytes - analysis</subject><subject>Zygote - analysis</subject><issn>0268-1161</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkF1LwzAUhoMoc05_gtAbvetMmqRpL7yYmzpx4IQJ4k1I0xPsXD-WrLD9eyMtA6_OgffjHB6EbggeE5zSu3pvapuv69ZWauPG321poRkrQgVOxQkaEhbjMKIcn6IhjuIkJCQm5-jCuTXGfk3iARp4nQjChyiYrJaBqvJgMlsGRRX4OlUFjYUQysweaneJzoy_A1f9HKGPp8fVdB4u3p5fppNFqCNBd2GaARc005nQmDBqUp3QlAFoJnIDgFWmWWJoTlPBdOLf4lxluYoZ4TQhRtERuu16G1tvW3A7WRZOw2ajKqhbJ0WaRJhF3BvvO6O2tXMWjGxsUSp7kATLP0LyPyHZEZI9IZ-_7g-1WQn5Md0j8XrY6YXbwf4oK_sjY0EFl_PPL_k6m67e5w9Czugvvgl3rw</recordid><startdate>199004</startdate><enddate>199004</enddate><creator>Slotte, Håkan</creator><creator>Gustafson, Ove</creator><creator>Nylund, Lars</creator><creator>Pousette, Åke</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199004</creationdate><title>ATP and ADP in human pre-embryos</title><author>Slotte, Håkan ; Gustafson, Ove ; Nylund, Lars ; Pousette, Åke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c273t-9be573bcb7c0143f9c8394eec47dfee0abc48f3d3974c826855abda6415381fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Adenosine Diphosphate - analysis</topic><topic>Adenosine Monophosphate - analysis</topic><topic>ADP</topic><topic>ATP</topic><topic>bioluminescence</topic><topic>Blastocyst - analysis</topic><topic>embryp</topic><topic>Humans</topic><topic>Morula - analysis</topic><topic>oocyte</topic><topic>Oocytes - analysis</topic><topic>Zygote - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Slotte, Håkan</creatorcontrib><creatorcontrib>Gustafson, Ove</creatorcontrib><creatorcontrib>Nylund, Lars</creatorcontrib><creatorcontrib>Pousette, Åke</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Slotte, Håkan</au><au>Gustafson, Ove</au><au>Nylund, Lars</au><au>Pousette, Åke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ATP and ADP in human pre-embryos</atitle><jtitle>Human reproduction (Oxford)</jtitle><addtitle>Hum Reprod</addtitle><date>1990-04</date><risdate>1990</risdate><volume>5</volume><issue>3</issue><spage>319</spage><epage>322</epage><pages>319-322</pages><issn>0268-1161</issn><eissn>1460-2350</eissn><abstract>Spare human oocytes and pre-embryos from an in-vitro fertilization (IVF) programme were individually analysed for ATP and ADP content using a bioluminescence method employing the firefly luceferin-luciferase reaction. The ATPcontent of oocytes that failed to fertilize in vitro was 1.71 ⊞ 0.28 pmol (n = 10), pronuclear stage ova1.93 ± 0.08 (n = 6), 2-cell stage 1.78 ± 0.20 (n = 7), 4-cell stage 1.73 ± 0.15 (n = 6), 6–8 cell stage 2.76 ± 0.53 (n = 8), morula stage 2.36 ± 0.68 (n = 8), early blastocyst stage 2.08 ± 0.25 (n = 7) and expanded blastocyst stage 2.26 ± 0.15 (n = 3) pmol. The ADP content of these pre-embryos was low in all stages with a small increment in the 2–4 cell stages and the blastocyst stage. This gave an elevated ATP/ADP ratio (range 19–92) indicating a goodenergy status. The sensitive luciferin-luciferase assay may be a tool for studying the energy status of spare oocytes and pre-embryos under different incubation conditions in human IVF programmes.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>2351715</pmid><doi>10.1093/oxfordjournals.humrep.a137097</doi><tpages>4</tpages></addata></record> |
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| ispartof | Human reproduction (Oxford), 1990-04, Vol.5 (3), p.319-322 |
| issn | 0268-1161 1460-2350 |
| language | eng |
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| source | MEDLINE; Oxford University Press Archive |
| subjects | Adenosine Diphosphate - analysis Adenosine Monophosphate - analysis ADP ATP bioluminescence Blastocyst - analysis embryp Humans Morula - analysis oocyte Oocytes - analysis Zygote - analysis |
| title | ATP and ADP in human pre-embryos |
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