Serotonergic activity in the rat striatum after intrastriatal transplantation of fetal nigra as measured by microdialysis
In vivo microdialysis was used to examine the effects of dopaminergic transplants on extracellular concentrations of dopamine (DA), serotonin (5-HT), and their precursors and major metabolites in the denervated rat striatum. Dialysis perfusates were collected from intact, 6-hydroxydopamine (6-OHDA)...
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| Veröffentlicht in: | Brain research 1998-03, Vol.788 (1), p.207-214 |
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| creator | Ishida, Yasushi Hashiguchi, Hiroyuki Todaka, Kazunari Kuwahara, Itsumi Ishizuka, Yuta Nakane, Hideyuki Uchimura, Daisuke Nishimori, Toshikazu Mitsuyama, Yoshio |
| description | In vivo microdialysis was used to examine the effects of dopaminergic transplants on extracellular concentrations of dopamine (DA), serotonin (5-HT), and their precursors and major metabolites in the denervated rat striatum. Dialysis perfusates were collected from intact, 6-hydroxydopamine (6-OHDA) lesion plus sham grafted, and lesion plus fetal substantia nigra (SN) grafted striata. The SN transplants ameliorated the reduction of striatal DA and dihydroxyphenylacetic acid (DOPAC) levels in rats with unilateral 6-OHDA lesions of the mesostriatal pathway. The transplants also increased extracellular levels of 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) in the denervated striatum. In response to NSD-1015 (an inhibitor of aromatic
l-amino acid decarboxylase), 5-hydroxytryptophan (5-HTP) levels were substantially elevated in the SN grafted striata as compared with those in the sham grafted controls, which continued even after subsequent administration of
l-3,4-dihydroxyphenylalanine (
l-DOPA, 100 mg/kg i.p.). Immunohistochemical analysis showed hyperinnervation of 5-HT fibers in the grafted striatum, which was consistent with the results of microdialysis experiments. These results indicated that implantation of SN grafts into the 6-OHDA-lesioned striatum of rats induces hyperactivity of 5-HT synthesis, release and metabolism. |
| doi_str_mv | 10.1016/S0006-8993(97)01541-2 |
| format | Article |
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l-amino acid decarboxylase), 5-hydroxytryptophan (5-HTP) levels were substantially elevated in the SN grafted striata as compared with those in the sham grafted controls, which continued even after subsequent administration of
l-3,4-dihydroxyphenylalanine (
l-DOPA, 100 mg/kg i.p.). Immunohistochemical analysis showed hyperinnervation of 5-HT fibers in the grafted striatum, which was consistent with the results of microdialysis experiments. These results indicated that implantation of SN grafts into the 6-OHDA-lesioned striatum of rats induces hyperactivity of 5-HT synthesis, release and metabolism.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/S0006-8993(97)01541-2</identifier><identifier>PMID: 9555017</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>5-Hydroxytryptophan ; 5-Hydroxytryptophan - metabolism ; 6-Hydroxydopamine ; Animals ; Aromatic Amino Acid Decarboxylase Inhibitors ; Biological and medical sciences ; Corpus Striatum ; Development. Senescence. Regeneration. Transplantation ; Enzyme Inhibitors - pharmacology ; Female ; Fetal Tissue Transplantation ; Fundamental and applied biological sciences. Psychology ; Hydrazines - pharmacology ; Hydroxyindoleacetic Acid - metabolism ; l-DOPA ; Levodopa - pharmacology ; Microdialysis ; Nigral transplants ; Oxidopamine ; Rat ; Rats ; Rats, Wistar ; Serotonin ; Serotonin - metabolism ; Serotonin - physiology ; Striatum ; Substantia Nigra - embryology ; Substantia Nigra - transplantation ; Transplantation, Heterotopic ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 1998-03, Vol.788 (1), p.207-214</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1998 INIST-CNRS</rights><rights>Copyright 1998 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-4fb7e20cbcf9316dea50b06d32afd4615bc1ae50d33acdcffcef8ddf269cc8c83</citedby><cites>FETCH-LOGICAL-c486t-4fb7e20cbcf9316dea50b06d32afd4615bc1ae50d33acdcffcef8ddf269cc8c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006899397015412$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2222127$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9555017$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ishida, Yasushi</creatorcontrib><creatorcontrib>Hashiguchi, Hiroyuki</creatorcontrib><creatorcontrib>Todaka, Kazunari</creatorcontrib><creatorcontrib>Kuwahara, Itsumi</creatorcontrib><creatorcontrib>Ishizuka, Yuta</creatorcontrib><creatorcontrib>Nakane, Hideyuki</creatorcontrib><creatorcontrib>Uchimura, Daisuke</creatorcontrib><creatorcontrib>Nishimori, Toshikazu</creatorcontrib><creatorcontrib>Mitsuyama, Yoshio</creatorcontrib><title>Serotonergic activity in the rat striatum after intrastriatal transplantation of fetal nigra as measured by microdialysis</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>In vivo microdialysis was used to examine the effects of dopaminergic transplants on extracellular concentrations of dopamine (DA), serotonin (5-HT), and their precursors and major metabolites in the denervated rat striatum. Dialysis perfusates were collected from intact, 6-hydroxydopamine (6-OHDA) lesion plus sham grafted, and lesion plus fetal substantia nigra (SN) grafted striata. The SN transplants ameliorated the reduction of striatal DA and dihydroxyphenylacetic acid (DOPAC) levels in rats with unilateral 6-OHDA lesions of the mesostriatal pathway. The transplants also increased extracellular levels of 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) in the denervated striatum. In response to NSD-1015 (an inhibitor of aromatic
l-amino acid decarboxylase), 5-hydroxytryptophan (5-HTP) levels were substantially elevated in the SN grafted striata as compared with those in the sham grafted controls, which continued even after subsequent administration of
l-3,4-dihydroxyphenylalanine (
l-DOPA, 100 mg/kg i.p.). Immunohistochemical analysis showed hyperinnervation of 5-HT fibers in the grafted striatum, which was consistent with the results of microdialysis experiments. These results indicated that implantation of SN grafts into the 6-OHDA-lesioned striatum of rats induces hyperactivity of 5-HT synthesis, release and metabolism.</description><subject>5-Hydroxytryptophan</subject><subject>5-Hydroxytryptophan - metabolism</subject><subject>6-Hydroxydopamine</subject><subject>Animals</subject><subject>Aromatic Amino Acid Decarboxylase Inhibitors</subject><subject>Biological and medical sciences</subject><subject>Corpus Striatum</subject><subject>Development. Senescence. Regeneration. Transplantation</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Female</subject><subject>Fetal Tissue Transplantation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrazines - pharmacology</subject><subject>Hydroxyindoleacetic Acid - metabolism</subject><subject>l-DOPA</subject><subject>Levodopa - pharmacology</subject><subject>Microdialysis</subject><subject>Nigral transplants</subject><subject>Oxidopamine</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Serotonin</subject><subject>Serotonin - metabolism</subject><subject>Serotonin - physiology</subject><subject>Striatum</subject><subject>Substantia Nigra - embryology</subject><subject>Substantia Nigra - transplantation</subject><subject>Transplantation, Heterotopic</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EKkPhJ1TyAiFYBGwnceIVqipeUiUWhbV1c31djPIYbKdS_j2ezmi29caP8137-hzGrqT4KIXUn-6EELrqjanfm-6DkG0jK_WM7WTfqUqrRjxnuzPykr1K6W_Z1rURF-zCtG0rZLdj2x3FJS8zxfuAHDCHh5A3Hmae_xCPkHnKMUBeJw4-UyxKjnA8g5GX9Zz2I8wZclhmvnju6SDM4T4Ch8QngrRGcnzY-BQwLi7AuKWQXrMXHsZEb07zJfv99cuvm-_V7c9vP26ubytsep2rxg8dKYEDelNL7QhaMQjtagXeNVq2A0qgVri6BnToPZLvnfNKG8Qe-_qSvTveu4_Lv5VStlNISGNpmpY12c700nSqeRKUWulG9QewPYLlNylF8nYfwwRxs1LYQzb2MRt7MN6azj5mY1Wpuzo9sA4TuXPVKYyivz3pkBBGX7zFkM6YKkOqA_b5iFFx7SFQtAkDzUguRMJs3RKeaOQ_fN-vlw</recordid><startdate>19980330</startdate><enddate>19980330</enddate><creator>Ishida, Yasushi</creator><creator>Hashiguchi, Hiroyuki</creator><creator>Todaka, Kazunari</creator><creator>Kuwahara, Itsumi</creator><creator>Ishizuka, Yuta</creator><creator>Nakane, Hideyuki</creator><creator>Uchimura, Daisuke</creator><creator>Nishimori, Toshikazu</creator><creator>Mitsuyama, Yoshio</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19980330</creationdate><title>Serotonergic activity in the rat striatum after intrastriatal transplantation of fetal nigra as measured by microdialysis</title><author>Ishida, Yasushi ; Hashiguchi, Hiroyuki ; Todaka, Kazunari ; Kuwahara, Itsumi ; Ishizuka, Yuta ; Nakane, Hideyuki ; Uchimura, Daisuke ; Nishimori, Toshikazu ; Mitsuyama, Yoshio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-4fb7e20cbcf9316dea50b06d32afd4615bc1ae50d33acdcffcef8ddf269cc8c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>5-Hydroxytryptophan</topic><topic>5-Hydroxytryptophan - metabolism</topic><topic>6-Hydroxydopamine</topic><topic>Animals</topic><topic>Aromatic Amino Acid Decarboxylase Inhibitors</topic><topic>Biological and medical sciences</topic><topic>Corpus Striatum</topic><topic>Development. Senescence. Regeneration. Transplantation</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Female</topic><topic>Fetal Tissue Transplantation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrazines - pharmacology</topic><topic>Hydroxyindoleacetic Acid - metabolism</topic><topic>l-DOPA</topic><topic>Levodopa - pharmacology</topic><topic>Microdialysis</topic><topic>Nigral transplants</topic><topic>Oxidopamine</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Serotonin</topic><topic>Serotonin - metabolism</topic><topic>Serotonin - physiology</topic><topic>Striatum</topic><topic>Substantia Nigra - embryology</topic><topic>Substantia Nigra - transplantation</topic><topic>Transplantation, Heterotopic</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ishida, Yasushi</creatorcontrib><creatorcontrib>Hashiguchi, Hiroyuki</creatorcontrib><creatorcontrib>Todaka, Kazunari</creatorcontrib><creatorcontrib>Kuwahara, Itsumi</creatorcontrib><creatorcontrib>Ishizuka, Yuta</creatorcontrib><creatorcontrib>Nakane, Hideyuki</creatorcontrib><creatorcontrib>Uchimura, Daisuke</creatorcontrib><creatorcontrib>Nishimori, Toshikazu</creatorcontrib><creatorcontrib>Mitsuyama, Yoshio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ishida, Yasushi</au><au>Hashiguchi, Hiroyuki</au><au>Todaka, Kazunari</au><au>Kuwahara, Itsumi</au><au>Ishizuka, Yuta</au><au>Nakane, Hideyuki</au><au>Uchimura, Daisuke</au><au>Nishimori, Toshikazu</au><au>Mitsuyama, Yoshio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serotonergic activity in the rat striatum after intrastriatal transplantation of fetal nigra as measured by microdialysis</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1998-03-30</date><risdate>1998</risdate><volume>788</volume><issue>1</issue><spage>207</spage><epage>214</epage><pages>207-214</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>In vivo microdialysis was used to examine the effects of dopaminergic transplants on extracellular concentrations of dopamine (DA), serotonin (5-HT), and their precursors and major metabolites in the denervated rat striatum. Dialysis perfusates were collected from intact, 6-hydroxydopamine (6-OHDA) lesion plus sham grafted, and lesion plus fetal substantia nigra (SN) grafted striata. The SN transplants ameliorated the reduction of striatal DA and dihydroxyphenylacetic acid (DOPAC) levels in rats with unilateral 6-OHDA lesions of the mesostriatal pathway. The transplants also increased extracellular levels of 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) in the denervated striatum. In response to NSD-1015 (an inhibitor of aromatic
l-amino acid decarboxylase), 5-hydroxytryptophan (5-HTP) levels were substantially elevated in the SN grafted striata as compared with those in the sham grafted controls, which continued even after subsequent administration of
l-3,4-dihydroxyphenylalanine (
l-DOPA, 100 mg/kg i.p.). Immunohistochemical analysis showed hyperinnervation of 5-HT fibers in the grafted striatum, which was consistent with the results of microdialysis experiments. These results indicated that implantation of SN grafts into the 6-OHDA-lesioned striatum of rats induces hyperactivity of 5-HT synthesis, release and metabolism.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>9555017</pmid><doi>10.1016/S0006-8993(97)01541-2</doi><tpages>8</tpages></addata></record> |
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| subjects | 5-Hydroxytryptophan 5-Hydroxytryptophan - metabolism 6-Hydroxydopamine Animals Aromatic Amino Acid Decarboxylase Inhibitors Biological and medical sciences Corpus Striatum Development. Senescence. Regeneration. Transplantation Enzyme Inhibitors - pharmacology Female Fetal Tissue Transplantation Fundamental and applied biological sciences. Psychology Hydrazines - pharmacology Hydroxyindoleacetic Acid - metabolism l-DOPA Levodopa - pharmacology Microdialysis Nigral transplants Oxidopamine Rat Rats Rats, Wistar Serotonin Serotonin - metabolism Serotonin - physiology Striatum Substantia Nigra - embryology Substantia Nigra - transplantation Transplantation, Heterotopic Vertebrates: nervous system and sense organs |
| title | Serotonergic activity in the rat striatum after intrastriatal transplantation of fetal nigra as measured by microdialysis |
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