Conversion of palmitate to unsaturated fatty acids differs in a Neurospora crassa mutant with impaired fatty acid synthase activity
The Neurospora crassa cel (fatty acid chain elongation) mutant has impaired fatty acid synthase activity. The cel mutant requires exogenous 16:0 for growth and converts 16:0 to other fatty acids. In contrast to wild‐type N. crassa, which converted only 42% of the exogenous [7,7,8,8‐2H4]16:0 that was...
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| Veröffentlicht in: | Lipids 1998-03, Vol.33 (3), p.303-306 |
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| creator | Stafford, A.E McKeon, T.A Goodrich-Tanrikulu, M |
| description | The Neurospora crassa cel (fatty acid chain elongation) mutant has impaired fatty acid synthase activity. The cel mutant requires exogenous 16:0 for growth and converts 16:0 to other fatty acids. In contrast to wild‐type N. crassa, which converted only 42% of the exogenous [7,7,8,8‐2H4]16:0 that was incorporated into cell lipids to unsaturated fatty acids, cel converted 72%. In addition, cel contains higher levels of 18:3δ9,12,15 than wild‐type, and synthesizes two fatty acids, 20:2δ11,14 and 20:3δ11,14,17, found at only trace levels in wild‐type. Thus, the Δ15‐desaturase activity and elongation activity on 18‐carbon polyunsaturated fatty acids are higher for cel than wild‐type. This altered metabolism of exogenous 16:0 may be directly due to impaired flux through the endogenous fatty acid biosynthetic pathway, or may result from altered regulation of the synthesis of unsaturated fatty acids in the mutant. |
| doi_str_mv | 10.1007/s11745-998-0209-7 |
| format | Article |
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The cel mutant requires exogenous 16:0 for growth and converts 16:0 to other fatty acids. In contrast to wild‐type N. crassa, which converted only 42% of the exogenous [7,7,8,8‐2H4]16:0 that was incorporated into cell lipids to unsaturated fatty acids, cel converted 72%. In addition, cel contains higher levels of 18:3δ9,12,15 than wild‐type, and synthesizes two fatty acids, 20:2δ11,14 and 20:3δ11,14,17, found at only trace levels in wild‐type. Thus, the Δ15‐desaturase activity and elongation activity on 18‐carbon polyunsaturated fatty acids are higher for cel than wild‐type. This altered metabolism of exogenous 16:0 may be directly due to impaired flux through the endogenous fatty acid biosynthetic pathway, or may result from altered regulation of the synthesis of unsaturated fatty acids in the mutant.</description><identifier>ISSN: 0024-4201</identifier><identifier>EISSN: 1558-9307</identifier><identifier>DOI: 10.1007/s11745-998-0209-7</identifier><identifier>PMID: 9560805</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer‐Verlag</publisher><subject>Fatty Acid Desaturases - metabolism ; Fatty Acid Synthases - deficiency ; Fatty Acid Synthases - genetics ; Fatty acids ; Fatty Acids - biosynthesis ; Lipids ; Mutants ; Neurospora crassa - enzymology ; Neurospora crassa - genetics ; Nutrition ; Palmitic Acid - metabolism ; plant biochemistry ; plant physiology ; Polyunsaturated fatty acids ; Trace levels ; Triglycerides - metabolism</subject><ispartof>Lipids, 1998-03, Vol.33 (3), p.303-306</ispartof><rights>1998 American Oil Chemists' Society (AOCS)</rights><rights>AOCS Press 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3953-b1951aab450830efa493c760e981bca499d34da4b8c36811b8d788075239899d3</citedby><cites>FETCH-LOGICAL-c3953-b1951aab450830efa493c760e981bca499d34da4b8c36811b8d788075239899d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1007%2Fs11745-998-0209-7$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1007%2Fs11745-998-0209-7$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9560805$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stafford, A.E</creatorcontrib><creatorcontrib>McKeon, T.A</creatorcontrib><creatorcontrib>Goodrich-Tanrikulu, M</creatorcontrib><title>Conversion of palmitate to unsaturated fatty acids differs in a Neurospora crassa mutant with impaired fatty acid synthase activity</title><title>Lipids</title><addtitle>Lipids</addtitle><description>The Neurospora crassa cel (fatty acid chain elongation) mutant has impaired fatty acid synthase activity. The cel mutant requires exogenous 16:0 for growth and converts 16:0 to other fatty acids. In contrast to wild‐type N. crassa, which converted only 42% of the exogenous [7,7,8,8‐2H4]16:0 that was incorporated into cell lipids to unsaturated fatty acids, cel converted 72%. In addition, cel contains higher levels of 18:3δ9,12,15 than wild‐type, and synthesizes two fatty acids, 20:2δ11,14 and 20:3δ11,14,17, found at only trace levels in wild‐type. Thus, the Δ15‐desaturase activity and elongation activity on 18‐carbon polyunsaturated fatty acids are higher for cel than wild‐type. This altered metabolism of exogenous 16:0 may be directly due to impaired flux through the endogenous fatty acid biosynthetic pathway, or may result from altered regulation of the synthesis of unsaturated fatty acids in the mutant.</description><subject>Fatty Acid Desaturases - metabolism</subject><subject>Fatty Acid Synthases - deficiency</subject><subject>Fatty Acid Synthases - genetics</subject><subject>Fatty acids</subject><subject>Fatty Acids - biosynthesis</subject><subject>Lipids</subject><subject>Mutants</subject><subject>Neurospora crassa - enzymology</subject><subject>Neurospora crassa - genetics</subject><subject>Nutrition</subject><subject>Palmitic Acid - metabolism</subject><subject>plant biochemistry</subject><subject>plant physiology</subject><subject>Polyunsaturated fatty acids</subject><subject>Trace levels</subject><subject>Triglycerides - metabolism</subject><issn>0024-4201</issn><issn>1558-9307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU2L1TAUhoso453RH-BCDC7cVU-apEmWckfHgYsKOutw2qZOhrapSTpD1_5xc-lF0I2r8PJ-kMNTFC8ovKUA8l2kVHJRaq1KqECX8lGxo0KoUjOQj4sdQMVLXgF9WpzHeJcl5VqcFWda1KBA7Ipfez_d2xCdn4jvyYzD6BImS5InyxQxLSGrjvSY0kqwdV0knev7XCFuIkg-2yX4OPuApA0YI5JxSTgl8uDSLXHjjC781SdxndItRptVcvcurc-KJz0O0T4_vRfFzccP3_efysOXq-v9-0PZMi1Y2VAtKGLDBSgGtkeuWStrsFrRps1Kd4x3yBvVslpR2qhOKgVSVEyro3lRvNl25-B_LjYmM7rY2mHAyfolGpmHaqhUDr7-J3jnlzDlvxmlFGVciWOIbqE23x-D7c0c3IhhNRTMkY7Z6JhMxxzpGJk7L0_DSzPa7k_jhCP7cvMf3GDX_w-aw_XXS2DAcvPV1uzRG_wRXDQ33zJ4lu-RNdeK_QanNqWb</recordid><startdate>199803</startdate><enddate>199803</enddate><creator>Stafford, A.E</creator><creator>McKeon, T.A</creator><creator>Goodrich-Tanrikulu, M</creator><general>Springer‐Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>199803</creationdate><title>Conversion of palmitate to unsaturated fatty acids differs in a Neurospora crassa mutant with impaired fatty acid synthase activity</title><author>Stafford, A.E ; McKeon, T.A ; Goodrich-Tanrikulu, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3953-b1951aab450830efa493c760e981bca499d34da4b8c36811b8d788075239899d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Fatty Acid Desaturases - metabolism</topic><topic>Fatty Acid Synthases - deficiency</topic><topic>Fatty Acid Synthases - genetics</topic><topic>Fatty acids</topic><topic>Fatty Acids - biosynthesis</topic><topic>Lipids</topic><topic>Mutants</topic><topic>Neurospora crassa - enzymology</topic><topic>Neurospora crassa - genetics</topic><topic>Nutrition</topic><topic>Palmitic Acid - metabolism</topic><topic>plant biochemistry</topic><topic>plant physiology</topic><topic>Polyunsaturated fatty acids</topic><topic>Trace levels</topic><topic>Triglycerides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stafford, A.E</creatorcontrib><creatorcontrib>McKeon, T.A</creatorcontrib><creatorcontrib>Goodrich-Tanrikulu, M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Lipids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stafford, A.E</au><au>McKeon, T.A</au><au>Goodrich-Tanrikulu, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conversion of palmitate to unsaturated fatty acids differs in a Neurospora crassa mutant with impaired fatty acid synthase activity</atitle><jtitle>Lipids</jtitle><addtitle>Lipids</addtitle><date>1998-03</date><risdate>1998</risdate><volume>33</volume><issue>3</issue><spage>303</spage><epage>306</epage><pages>303-306</pages><issn>0024-4201</issn><eissn>1558-9307</eissn><abstract>The Neurospora crassa cel (fatty acid chain elongation) mutant has impaired fatty acid synthase activity. The cel mutant requires exogenous 16:0 for growth and converts 16:0 to other fatty acids. In contrast to wild‐type N. crassa, which converted only 42% of the exogenous [7,7,8,8‐2H4]16:0 that was incorporated into cell lipids to unsaturated fatty acids, cel converted 72%. In addition, cel contains higher levels of 18:3δ9,12,15 than wild‐type, and synthesizes two fatty acids, 20:2δ11,14 and 20:3δ11,14,17, found at only trace levels in wild‐type. Thus, the Δ15‐desaturase activity and elongation activity on 18‐carbon polyunsaturated fatty acids are higher for cel than wild‐type. This altered metabolism of exogenous 16:0 may be directly due to impaired flux through the endogenous fatty acid biosynthetic pathway, or may result from altered regulation of the synthesis of unsaturated fatty acids in the mutant.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer‐Verlag</pub><pmid>9560805</pmid><doi>10.1007/s11745-998-0209-7</doi><tpages>4</tpages></addata></record> |
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| ispartof | Lipids, 1998-03, Vol.33 (3), p.303-306 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals; SpringerLink Journals - AutoHoldings |
| subjects | Fatty Acid Desaturases - metabolism Fatty Acid Synthases - deficiency Fatty Acid Synthases - genetics Fatty acids Fatty Acids - biosynthesis Lipids Mutants Neurospora crassa - enzymology Neurospora crassa - genetics Nutrition Palmitic Acid - metabolism plant biochemistry plant physiology Polyunsaturated fatty acids Trace levels Triglycerides - metabolism |
| title | Conversion of palmitate to unsaturated fatty acids differs in a Neurospora crassa mutant with impaired fatty acid synthase activity |
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