Substitution of 5-methylcytosines for cytosines enhances the stability of topoisomerase I-DNA complexes and modulates the sequence selectivity of camptothecin-induced DNA cleavage
We have investigated the binding and cleavage of DNA by human topoisomerase I using a 160 bp restriction fragment containing either natural bases or 5-methylcytosine residues in place of cytosines. Experiments were performed in the presence and absence of the antitumour drug camptothecin which speci...
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| Veröffentlicht in: | FEBS letters 1998-03, Vol.425 (2), p.337-340 |
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| creator | Carrasco, Carolina Waring, Michael J Bailly, Christian |
| description | We have investigated the binding and cleavage of DNA by human topoisomerase I using a 160 bp restriction fragment containing either natural bases or 5-methylcytosine residues in place of cytosines. Experiments were performed in the presence and absence of the antitumour drug camptothecin which specifically inhibits topoisomerase I. Replacement of all cytosines with 5-methylcytosine residues (i) reinforces the enzyme-DNA interaction, (ii) enhances the stability of topoisomerase I-DNA complexes and (iii) modulates the sequence selectivity of camptothecin-induced DNA cleavage. The methyl group exposed in the major groove of the double helix is identified as a critical element for the interaction between topoisomerase I and DNA. |
| doi_str_mv | 10.1016/S0014-5793(98)00259-2 |
| format | Article |
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Experiments were performed in the presence and absence of the antitumour drug camptothecin which specifically inhibits topoisomerase I. Replacement of all cytosines with 5-methylcytosine residues (i) reinforces the enzyme-DNA interaction, (ii) enhances the stability of topoisomerase I-DNA complexes and (iii) modulates the sequence selectivity of camptothecin-induced DNA cleavage. The methyl group exposed in the major groove of the double helix is identified as a critical element for the interaction between topoisomerase I and DNA.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(98)00259-2</identifier><identifier>PMID: 9559675</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>5-Methylcytosine ; Base Sequence ; Camptothecin ; Camptothecin - metabolism ; Cytosine - analogs & derivatives ; Cytosine - metabolism ; DNA - metabolism ; DNA Topoisomerases, Type I - metabolism ; Humans ; Methylcytosine ; Molecular Sequence Data ; SDS, sodium dodecyl sulphate ; Structure-Activity Relationship ; Topoisomerase</subject><ispartof>FEBS letters, 1998-03, Vol.425 (2), p.337-340</ispartof><rights>1998 Federation of European Biochemical Societies</rights><rights>FEBS Letters 425 (1998) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4722-2a14014a08d838738881ec711ed9283852784d459856c29dcb7fae551f81317d3</citedby><cites>FETCH-LOGICAL-c4722-2a14014a08d838738881ec711ed9283852784d459856c29dcb7fae551f81317d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2898%2900259-2$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579398002592$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3537,27903,27904,45553,45554,46387,46811,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9559675$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carrasco, Carolina</creatorcontrib><creatorcontrib>Waring, Michael J</creatorcontrib><creatorcontrib>Bailly, Christian</creatorcontrib><title>Substitution of 5-methylcytosines for cytosines enhances the stability of topoisomerase I-DNA complexes and modulates the sequence selectivity of camptothecin-induced DNA cleavage</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We have investigated the binding and cleavage of DNA by human topoisomerase I using a 160 bp restriction fragment containing either natural bases or 5-methylcytosine residues in place of cytosines. Experiments were performed in the presence and absence of the antitumour drug camptothecin which specifically inhibits topoisomerase I. Replacement of all cytosines with 5-methylcytosine residues (i) reinforces the enzyme-DNA interaction, (ii) enhances the stability of topoisomerase I-DNA complexes and (iii) modulates the sequence selectivity of camptothecin-induced DNA cleavage. The methyl group exposed in the major groove of the double helix is identified as a critical element for the interaction between topoisomerase I and DNA.</description><subject>5-Methylcytosine</subject><subject>Base Sequence</subject><subject>Camptothecin</subject><subject>Camptothecin - metabolism</subject><subject>Cytosine - analogs & derivatives</subject><subject>Cytosine - metabolism</subject><subject>DNA - metabolism</subject><subject>DNA Topoisomerases, Type I - metabolism</subject><subject>Humans</subject><subject>Methylcytosine</subject><subject>Molecular Sequence Data</subject><subject>SDS, sodium dodecyl sulphate</subject><subject>Structure-Activity Relationship</subject><subject>Topoisomerase</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUcFu1DAQtRBVWQqfUCknBIdQ24k39gm1paWVKjgUzpbXnrBGThxsZyHfxQ_iZJdybE-emffmzXgeQqcEvyeYrM_uMSZ1yRpRvRX8HcaUiZI-QyvCm6qs6jV_jlYPlBfoZYw_cM45EcfoWDAm1g1boT_34yYmm8ZkfV_4tmBlB2k7OT0lH20PsWh9KP5n0G9Vr3OQtlDEpDbW2TTNnckP3kbfQVARitvy4-fzQvtucPA701Vvis6b0an0rxl-jpClcuBAJ7s76GjVDclnhrZ9aXszajDFIuZA7dR3eIWOWuUivD68J-jb9dXXy5vy7sun28vzu1LXDaUlVaTO_1eYG17lo3DOCeiGEDCC5gqjDa9NzQRna02F0ZumVcAYaTmpSGOqE_RmrzsEn1eNSXY2anBO9eDHKBvBCeG1yES2J-rgYwzQyiHYToVJEixns-RilpydkILLxSxJc9_pYcC46cA8dB3cyfjNHv9lHUxPE5XXVxd0QWZA8KU8j_qwl4J8sJ2FIKO28_WNDfn40nj7yLJ_AWFxu5c</recordid><startdate>19980327</startdate><enddate>19980327</enddate><creator>Carrasco, Carolina</creator><creator>Waring, Michael J</creator><creator>Bailly, Christian</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980327</creationdate><title>Substitution of 5-methylcytosines for cytosines enhances the stability of topoisomerase I-DNA complexes and modulates the sequence selectivity of camptothecin-induced DNA cleavage</title><author>Carrasco, Carolina ; Waring, Michael J ; Bailly, Christian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4722-2a14014a08d838738881ec711ed9283852784d459856c29dcb7fae551f81317d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>5-Methylcytosine</topic><topic>Base Sequence</topic><topic>Camptothecin</topic><topic>Camptothecin - metabolism</topic><topic>Cytosine - analogs & derivatives</topic><topic>Cytosine - metabolism</topic><topic>DNA - metabolism</topic><topic>DNA Topoisomerases, Type I - metabolism</topic><topic>Humans</topic><topic>Methylcytosine</topic><topic>Molecular Sequence Data</topic><topic>SDS, sodium dodecyl sulphate</topic><topic>Structure-Activity Relationship</topic><topic>Topoisomerase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carrasco, Carolina</creatorcontrib><creatorcontrib>Waring, Michael J</creatorcontrib><creatorcontrib>Bailly, Christian</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carrasco, Carolina</au><au>Waring, Michael J</au><au>Bailly, Christian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Substitution of 5-methylcytosines for cytosines enhances the stability of topoisomerase I-DNA complexes and modulates the sequence selectivity of camptothecin-induced DNA cleavage</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1998-03-27</date><risdate>1998</risdate><volume>425</volume><issue>2</issue><spage>337</spage><epage>340</epage><pages>337-340</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>We have investigated the binding and cleavage of DNA by human topoisomerase I using a 160 bp restriction fragment containing either natural bases or 5-methylcytosine residues in place of cytosines. Experiments were performed in the presence and absence of the antitumour drug camptothecin which specifically inhibits topoisomerase I. Replacement of all cytosines with 5-methylcytosine residues (i) reinforces the enzyme-DNA interaction, (ii) enhances the stability of topoisomerase I-DNA complexes and (iii) modulates the sequence selectivity of camptothecin-induced DNA cleavage. The methyl group exposed in the major groove of the double helix is identified as a critical element for the interaction between topoisomerase I and DNA.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>9559675</pmid><doi>10.1016/S0014-5793(98)00259-2</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1998-03, Vol.425 (2), p.337-340 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79811849 |
| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 5-Methylcytosine Base Sequence Camptothecin Camptothecin - metabolism Cytosine - analogs & derivatives Cytosine - metabolism DNA - metabolism DNA Topoisomerases, Type I - metabolism Humans Methylcytosine Molecular Sequence Data SDS, sodium dodecyl sulphate Structure-Activity Relationship Topoisomerase |
| title | Substitution of 5-methylcytosines for cytosines enhances the stability of topoisomerase I-DNA complexes and modulates the sequence selectivity of camptothecin-induced DNA cleavage |
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