Development of a PCR test based on a gene region associated with the pathogenicity of Pasteurella multocida serotype B:2, the causal agent of Haemorrhagic Septicaemia in Asia
We have developed a PCR assay to detect Pasteurella multocida serotype B:2, the causal agent of Haemorrhagic Septicaemia (HS) in Asia. Nucleotide sequence determination of a 16S rRNA–23S rRNA PCR product unique to B:2 strains was shown to share amino acid sequence homology with a bacteriophage Mu pr...
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| Veröffentlicht in: | Veterinary microbiology 1998-01, Vol.59 (4), p.295-307 |
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| creator | Brickell, S.K. Thomas, L.M. Long, K.A. Panaccio, M. Widders, P.R. |
| description | We have developed a PCR assay to detect
Pasteurella multocida serotype B:2, the causal agent of Haemorrhagic Septicaemia (HS) in Asia. Nucleotide sequence determination of a 16S rRNA–23S rRNA PCR product unique to B:2 strains was shown to share amino acid sequence homology with a bacteriophage Mu protein. Primers designed from this sequence when tested against a panel of isolates recovered from a wide geographical area and representing a large range of bacterial genera and species, were found to specifically amplify DNA from
P. multocida, serotype B:2. Southern hybridisation confirmed the presence of this sequence in only the B:2 serotype of
P. multocida, suggesting an association between bacterial virulence and the presence of bacteriophage genes in the bacterial genome. The results of this study demonstrate the potential application of PCR to the diagnosis of HS in cattle and buffalo in Asia. Application of PCR to support diagnosis of HS will greatly improve accuracy, laboratory response time, and will facilitate rational deployment of resources for controlling this disease. |
| doi_str_mv | 10.1016/S0378-1135(97)00199-5 |
| format | Article |
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Pasteurella multocida serotype B:2, the causal agent of Haemorrhagic Septicaemia (HS) in Asia. Nucleotide sequence determination of a 16S rRNA–23S rRNA PCR product unique to B:2 strains was shown to share amino acid sequence homology with a bacteriophage Mu protein. Primers designed from this sequence when tested against a panel of isolates recovered from a wide geographical area and representing a large range of bacterial genera and species, were found to specifically amplify DNA from
P. multocida, serotype B:2. Southern hybridisation confirmed the presence of this sequence in only the B:2 serotype of
P. multocida, suggesting an association between bacterial virulence and the presence of bacteriophage genes in the bacterial genome. The results of this study demonstrate the potential application of PCR to the diagnosis of HS in cattle and buffalo in Asia. Application of PCR to support diagnosis of HS will greatly improve accuracy, laboratory response time, and will facilitate rational deployment of resources for controlling this disease.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/S0378-1135(97)00199-5</identifier><identifier>PMID: 9556860</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; ASIA ; ASIE ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Blotting, Southern - veterinary ; BOVIN ; BUFALO DE AGUA ; Buffaloes ; BUFFLE D'EAU ; CATTLE ; Cattle Diseases - diagnosis ; Cattle Diseases - microbiology ; DIAGNOSIS ; Diagnosis—bacteria ; DIAGNOSTIC ; DIAGNOSTICO ; DNA Primers - chemistry ; DNA, Bacterial - chemistry ; Electrophoresis, Agar Gel - veterinary ; Enzyme-Linked Immunosorbent Assay - veterinary ; Fundamental and applied biological sciences. Psychology ; GANADO BOVINO ; HAEMORRHAGIC SEPTICAEMIA ; Hemorrhagic Septicemia - diagnosis ; Hemorrhagic Septicemia - microbiology ; Hemorrhagic Septicemia - veterinary ; Microbiology ; Molecular Sequence Data ; NUCLEOTIDE SEQUENCE ; PASTEURELLA MULTOCIDA ; Pasteurella multocida - classification ; Pasteurella multocida - genetics ; Pasteurella multocida - pathogenicity ; PCR ; Polymerase Chain Reaction - veterinary ; RNA, Ribosomal, 16S - genetics ; RNA, Ribosomal, 23S - genetics ; SECUENCIA NUCLEOTIDICA ; SEPTICEMIA HEMORRAGICA ; SEPTICEMIE HEMORRAGIQUE ; SEQUENCE NUCLEOTIDIQUE ; Serotyping - veterinary ; WATER BUFFALOES</subject><ispartof>Veterinary microbiology, 1998-01, Vol.59 (4), p.295-307</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-167f8f1baf5dba66587d550459c987c29c26abbbd2cc7a1eddef7c4800f03e533</citedby><cites>FETCH-LOGICAL-c374t-167f8f1baf5dba66587d550459c987c29c26abbbd2cc7a1eddef7c4800f03e533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378113597001995$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2226493$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9556860$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brickell, S.K.</creatorcontrib><creatorcontrib>Thomas, L.M.</creatorcontrib><creatorcontrib>Long, K.A.</creatorcontrib><creatorcontrib>Panaccio, M.</creatorcontrib><creatorcontrib>Widders, P.R.</creatorcontrib><title>Development of a PCR test based on a gene region associated with the pathogenicity of Pasteurella multocida serotype B:2, the causal agent of Haemorrhagic Septicaemia in Asia</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>We have developed a PCR assay to detect
Pasteurella multocida serotype B:2, the causal agent of Haemorrhagic Septicaemia (HS) in Asia. Nucleotide sequence determination of a 16S rRNA–23S rRNA PCR product unique to B:2 strains was shown to share amino acid sequence homology with a bacteriophage Mu protein. Primers designed from this sequence when tested against a panel of isolates recovered from a wide geographical area and representing a large range of bacterial genera and species, were found to specifically amplify DNA from
P. multocida, serotype B:2. Southern hybridisation confirmed the presence of this sequence in only the B:2 serotype of
P. multocida, suggesting an association between bacterial virulence and the presence of bacteriophage genes in the bacterial genome. The results of this study demonstrate the potential application of PCR to the diagnosis of HS in cattle and buffalo in Asia. Application of PCR to support diagnosis of HS will greatly improve accuracy, laboratory response time, and will facilitate rational deployment of resources for controlling this disease.</description><subject>Animals</subject><subject>ASIA</subject><subject>ASIE</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern - veterinary</subject><subject>BOVIN</subject><subject>BUFALO DE AGUA</subject><subject>Buffaloes</subject><subject>BUFFLE D'EAU</subject><subject>CATTLE</subject><subject>Cattle Diseases - diagnosis</subject><subject>Cattle Diseases - microbiology</subject><subject>DIAGNOSIS</subject><subject>Diagnosis—bacteria</subject><subject>DIAGNOSTIC</subject><subject>DIAGNOSTICO</subject><subject>DNA Primers - chemistry</subject><subject>DNA, Bacterial - chemistry</subject><subject>Electrophoresis, Agar Gel - veterinary</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GANADO BOVINO</subject><subject>HAEMORRHAGIC SEPTICAEMIA</subject><subject>Hemorrhagic Septicemia - diagnosis</subject><subject>Hemorrhagic Septicemia - microbiology</subject><subject>Hemorrhagic Septicemia - veterinary</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>PASTEURELLA MULTOCIDA</subject><subject>Pasteurella multocida - classification</subject><subject>Pasteurella multocida - genetics</subject><subject>Pasteurella multocida - pathogenicity</subject><subject>PCR</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>RNA, Ribosomal, 23S - genetics</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>SEPTICEMIA HEMORRAGICA</subject><subject>SEPTICEMIE HEMORRAGIQUE</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>Serotyping - veterinary</subject><subject>WATER BUFFALOES</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUV1v1DAQjBCoHIWfUMkPCIFEwE7iOO4LKsdHkU5QUXi2Ns4mZ5TEwXaK7k_xG3HuTsdjnyzvzOzs7iTJBaNvGGXl21uaiyplLOcvpXhFKZMy5Q-SFatEnma8yB4mqxPlcfLE-1-U0kKW9Cw5k5yXVUlXyd8PeIe9nQYcA7EtAXKz_k4C-kBq8NgQO8ZahyMSh51Zft5bbSBE7I8JWxK2SCYIWxtJRpuwW9rcgA84O-x7IMPch6hogHh0NuwmJO8vs9d7oYbZQ0-gO9pfAw7WuS10RpNbnILRsWKAmJFceQNPk0ct9B6fHd_z5Oenjz_W1-nm2-cv66tNqnNRhJSVoq1aVkPLmxrKklei4ZwWXGpZCZ1JnZVQ13WTaS2AYdNgK3RRUdrSHHmenycvDn0nZ3_P8RpqMF4v64xoZ6-ErBjN46XvI7KS85yLIhL5gaid9d5hqyZnBnA7xahaAlX7QNWSlpJC7QNVPOoujgZzPWBzUh0TjPjzIw5eQ986GLXxJ1qWZWUh8_9tWrAKOhcpXzfRIa7Miv147w44xqveGXTKa4OjxsY41EE11twz6D_NUMfy</recordid><startdate>19980131</startdate><enddate>19980131</enddate><creator>Brickell, S.K.</creator><creator>Thomas, L.M.</creator><creator>Long, K.A.</creator><creator>Panaccio, M.</creator><creator>Widders, P.R.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19980131</creationdate><title>Development of a PCR test based on a gene region associated with the pathogenicity of Pasteurella multocida serotype B:2, the causal agent of Haemorrhagic Septicaemia in Asia</title><author>Brickell, S.K. ; Thomas, L.M. ; Long, K.A. ; Panaccio, M. ; Widders, P.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-167f8f1baf5dba66587d550459c987c29c26abbbd2cc7a1eddef7c4800f03e533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>ASIA</topic><topic>ASIE</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern - veterinary</topic><topic>BOVIN</topic><topic>BUFALO DE AGUA</topic><topic>Buffaloes</topic><topic>BUFFLE D'EAU</topic><topic>CATTLE</topic><topic>Cattle Diseases - diagnosis</topic><topic>Cattle Diseases - microbiology</topic><topic>DIAGNOSIS</topic><topic>Diagnosis—bacteria</topic><topic>DIAGNOSTIC</topic><topic>DIAGNOSTICO</topic><topic>DNA Primers - chemistry</topic><topic>DNA, Bacterial - chemistry</topic><topic>Electrophoresis, Agar Gel - veterinary</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GANADO BOVINO</topic><topic>HAEMORRHAGIC SEPTICAEMIA</topic><topic>Hemorrhagic Septicemia - diagnosis</topic><topic>Hemorrhagic Septicemia - microbiology</topic><topic>Hemorrhagic Septicemia - veterinary</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>PASTEURELLA MULTOCIDA</topic><topic>Pasteurella multocida - classification</topic><topic>Pasteurella multocida - genetics</topic><topic>Pasteurella multocida - pathogenicity</topic><topic>PCR</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>RNA, Ribosomal, 23S - genetics</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>SEPTICEMIA HEMORRAGICA</topic><topic>SEPTICEMIE HEMORRAGIQUE</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>Serotyping - veterinary</topic><topic>WATER BUFFALOES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brickell, S.K.</creatorcontrib><creatorcontrib>Thomas, L.M.</creatorcontrib><creatorcontrib>Long, K.A.</creatorcontrib><creatorcontrib>Panaccio, M.</creatorcontrib><creatorcontrib>Widders, P.R.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brickell, S.K.</au><au>Thomas, L.M.</au><au>Long, K.A.</au><au>Panaccio, M.</au><au>Widders, P.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a PCR test based on a gene region associated with the pathogenicity of Pasteurella multocida serotype B:2, the causal agent of Haemorrhagic Septicaemia in Asia</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>1998-01-31</date><risdate>1998</risdate><volume>59</volume><issue>4</issue><spage>295</spage><epage>307</epage><pages>295-307</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>We have developed a PCR assay to detect
Pasteurella multocida serotype B:2, the causal agent of Haemorrhagic Septicaemia (HS) in Asia. Nucleotide sequence determination of a 16S rRNA–23S rRNA PCR product unique to B:2 strains was shown to share amino acid sequence homology with a bacteriophage Mu protein. Primers designed from this sequence when tested against a panel of isolates recovered from a wide geographical area and representing a large range of bacterial genera and species, were found to specifically amplify DNA from
P. multocida, serotype B:2. Southern hybridisation confirmed the presence of this sequence in only the B:2 serotype of
P. multocida, suggesting an association between bacterial virulence and the presence of bacteriophage genes in the bacterial genome. The results of this study demonstrate the potential application of PCR to the diagnosis of HS in cattle and buffalo in Asia. Application of PCR to support diagnosis of HS will greatly improve accuracy, laboratory response time, and will facilitate rational deployment of resources for controlling this disease.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>9556860</pmid><doi>10.1016/S0378-1135(97)00199-5</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Veterinary microbiology, 1998-01, Vol.59 (4), p.295-307 |
| issn | 0378-1135 1873-2542 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals ASIA ASIE Bacteriological methods and techniques used in bacteriology Bacteriology Base Sequence Biological and medical sciences Blotting, Southern - veterinary BOVIN BUFALO DE AGUA Buffaloes BUFFLE D'EAU CATTLE Cattle Diseases - diagnosis Cattle Diseases - microbiology DIAGNOSIS Diagnosis—bacteria DIAGNOSTIC DIAGNOSTICO DNA Primers - chemistry DNA, Bacterial - chemistry Electrophoresis, Agar Gel - veterinary Enzyme-Linked Immunosorbent Assay - veterinary Fundamental and applied biological sciences. Psychology GANADO BOVINO HAEMORRHAGIC SEPTICAEMIA Hemorrhagic Septicemia - diagnosis Hemorrhagic Septicemia - microbiology Hemorrhagic Septicemia - veterinary Microbiology Molecular Sequence Data NUCLEOTIDE SEQUENCE PASTEURELLA MULTOCIDA Pasteurella multocida - classification Pasteurella multocida - genetics Pasteurella multocida - pathogenicity PCR Polymerase Chain Reaction - veterinary RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 23S - genetics SECUENCIA NUCLEOTIDICA SEPTICEMIA HEMORRAGICA SEPTICEMIE HEMORRAGIQUE SEQUENCE NUCLEOTIDIQUE Serotyping - veterinary WATER BUFFALOES |
| title | Development of a PCR test based on a gene region associated with the pathogenicity of Pasteurella multocida serotype B:2, the causal agent of Haemorrhagic Septicaemia in Asia |
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