Affinity mode of capillary isoelectric focusing for the characterization of the biotin-binding protein actinavidin

Different modes of capillary isoelectric focusing (CIEF) with salt mobilization and zwitterionic additive to cathodic mobilizer were applied to characterize the biotin‐binding protein actinavidin and its affinity properties. The analysis is performed in a neutral coated capillary with completely eli...

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Veröffentlicht in:	Electrophoresis 1998-03, Vol.19 (3), p.427-432
1. Verfasser:	Okun, Vadim M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinavidin Affinity Affinity Labels Bacterial Proteins - chemistry Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Base Sequence Biotin Biotin - metabolism Capillary isoelectric focusing Carrier Proteins - chemistry Carrier Proteins - isolation & purification Carrier Proteins - metabolism Electrophoresis, Capillary - methods Isoelectric Focusing - methods Isoelectric Point Oligodeoxyribonucleotides - chemistry Protein Denaturation Streptomyces - chemistry
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