Determinants of species- and placenta-specific expression of p100 GAP
This study was based on the hypothesis that both primary sequence and methylation status of the GTPase activating protein (GAP) gene limits expression of p100 GAP to primate placenta. Due to alternate splicing, a 65-bp insert appears between the first and second coding exons of p100 GAP mRNA, and tr...
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| Veröffentlicht in: | Placenta (Eastbourne) 1998-03, Vol.19 (2), p.225-230 |
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| creator | Liao, L. Trouba, K.J. Vorce, R.L. |
| description | This study was based on the hypothesis that both primary sequence and methylation status of the GTPase activating protein (GAP) gene limits expression of p100 GAP to primate placenta. Due to alternate splicing, a 65-bp insert appears between the first and second coding exons of p100 GAP mRNA, and translation of p100 GAP initiates within this insert. Examination of the sequence surrounding the 65-bp insert revealed that the monkey GAP gene contained both the 3′ splice donor site and the internal start codon, whereas the mouse GAP gene contained neither. To address p100 GAP tissue specificity, the methylation status of the GAP gene was examined. Site-specific demethylation was found to correlate with synthesis of p100 GAP, suggesting that methylation regulates the expression of different GAP isoforms. The results of this study provide a mechanistic basis for the observation that p100 GAP is synthesized only in primate placenta and suggest that its expression is regulated, in part, by gene methylation. |
| doi_str_mv | 10.1016/S0143-4004(98)90012-1 |
| format | Article |
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Due to alternate splicing, a 65-bp insert appears between the first and second coding exons of p100 GAP mRNA, and translation of p100 GAP initiates within this insert. Examination of the sequence surrounding the 65-bp insert revealed that the monkey GAP gene contained both the 3′ splice donor site and the internal start codon, whereas the mouse GAP gene contained neither. To address p100 GAP tissue specificity, the methylation status of the GAP gene was examined. Site-specific demethylation was found to correlate with synthesis of p100 GAP, suggesting that methylation regulates the expression of different GAP isoforms. The results of this study provide a mechanistic basis for the observation that p100 GAP is synthesized only in primate placenta and suggest that its expression is regulated, in part, by gene methylation.</description><identifier>ISSN: 0143-4004</identifier><identifier>EISSN: 1532-3102</identifier><identifier>DOI: 10.1016/S0143-4004(98)90012-1</identifier><identifier>PMID: 9548190</identifier><identifier>CODEN: PLACDF</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adult ; Animals ; Base Sequence ; Biological and medical sciences ; DNA - analysis ; DNA Methylation ; Embryology: invertebrates and vertebrates. Teratology ; Female ; Fetal membranes ; Fibroblasts - enzymology ; Fundamental and applied biological sciences. Psychology ; General aspects. Development. Fetal membranes ; GTP Phosphohydrolases - genetics ; GTP Phosphohydrolases - metabolism ; GTPase-Activating Proteins ; Haplorhini ; Humans ; Male ; Mice ; Molecular Sequence Data ; Placenta - enzymology ; Polymerase Chain Reaction ; Pregnancy ; Proteins - genetics ; Proteins - metabolism ; Rats ; Rats, Sprague-Dawley ; Sequence Homology, Nucleic Acid ; Species Specificity ; Tumor Cells, Cultured - enzymology</subject><ispartof>Placenta (Eastbourne), 1998-03, Vol.19 (2), p.225-230</ispartof><rights>1998</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-40b453680067e6b1edec19a3091a4b9f28cdf00251c3ed5dee98fdd4dc49a3653</citedby><cites>FETCH-LOGICAL-c389t-40b453680067e6b1edec19a3091a4b9f28cdf00251c3ed5dee98fdd4dc49a3653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0143-4004(98)90012-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2202003$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9548190$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liao, L.</creatorcontrib><creatorcontrib>Trouba, K.J.</creatorcontrib><creatorcontrib>Vorce, R.L.</creatorcontrib><title>Determinants of species- and placenta-specific expression of p100 GAP</title><title>Placenta (Eastbourne)</title><addtitle>Placenta</addtitle><description>This study was based on the hypothesis that both primary sequence and methylation status of the GTPase activating protein (GAP) gene limits expression of p100 GAP to primate placenta. Due to alternate splicing, a 65-bp insert appears between the first and second coding exons of p100 GAP mRNA, and translation of p100 GAP initiates within this insert. Examination of the sequence surrounding the 65-bp insert revealed that the monkey GAP gene contained both the 3′ splice donor site and the internal start codon, whereas the mouse GAP gene contained neither. To address p100 GAP tissue specificity, the methylation status of the GAP gene was examined. Site-specific demethylation was found to correlate with synthesis of p100 GAP, suggesting that methylation regulates the expression of different GAP isoforms. The results of this study provide a mechanistic basis for the observation that p100 GAP is synthesized only in primate placenta and suggest that its expression is regulated, in part, by gene methylation.</description><subject>Adult</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA - analysis</subject><subject>DNA Methylation</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>Fetal membranes</subject><subject>Fibroblasts - enzymology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Development. Fetal membranes</subject><subject>GTP Phosphohydrolases - genetics</subject><subject>GTP Phosphohydrolases - metabolism</subject><subject>GTPase-Activating Proteins</subject><subject>Haplorhini</subject><subject>Humans</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Placenta - enzymology</subject><subject>Polymerase Chain Reaction</subject><subject>Pregnancy</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Species Specificity</subject><subject>Tumor Cells, Cultured - enzymology</subject><issn>0143-4004</issn><issn>1532-3102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkN9LwzAQgIMoOqd_gtAHEX2o3iVpbZ5kzDkFQUF9DllyhUjX1qQT_e9tt7FXuYeDu-9-8DF2hnCNgPnNG6AUqQSQl6q4UgDIU9xjI8wETwUC32ejHXLEjmP8BAAlkR-yQ5XJAhWM2OyeOgpLX5u6i0lTJrEl6ymmiald0lbGUt2ZdF0tvU3opw0Uo2_qAW4RIJlPXk_YQWmqSKfbPGYfD7P36WP6_DJ_mk6eUysK1fWfLGQm8gIgv6V8geTIojICFBq5UCUvrCsBeIZWkMsckSpK56SzsqfyTIzZxWZvG5qvFcVOL320VFWmpmYV9a3qA7jowWwD2tDEGKjUbfBLE341gh706bU-PbjRqtBrfRr7ubPtgdViSW43tfXV98-3fROtqcpgauvjDuMcOMBw_m6DUS_j21PQsZdaW3I-kO20a_w_j_wBGnyKXA</recordid><startdate>19980301</startdate><enddate>19980301</enddate><creator>Liao, L.</creator><creator>Trouba, K.J.</creator><creator>Vorce, R.L.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980301</creationdate><title>Determinants of species- and placenta-specific expression of p100 GAP</title><author>Liao, L. ; Trouba, K.J. ; Vorce, R.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-40b453680067e6b1edec19a3091a4b9f28cdf00251c3ed5dee98fdd4dc49a3653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA - analysis</topic><topic>DNA Methylation</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Female</topic><topic>Fetal membranes</topic><topic>Fibroblasts - enzymology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Development. Fetal membranes</topic><topic>GTP Phosphohydrolases - genetics</topic><topic>GTP Phosphohydrolases - metabolism</topic><topic>GTPase-Activating Proteins</topic><topic>Haplorhini</topic><topic>Humans</topic><topic>Male</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Placenta - enzymology</topic><topic>Polymerase Chain Reaction</topic><topic>Pregnancy</topic><topic>Proteins - genetics</topic><topic>Proteins - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Species Specificity</topic><topic>Tumor Cells, Cultured - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liao, L.</creatorcontrib><creatorcontrib>Trouba, K.J.</creatorcontrib><creatorcontrib>Vorce, R.L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Placenta (Eastbourne)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liao, L.</au><au>Trouba, K.J.</au><au>Vorce, R.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determinants of species- and placenta-specific expression of p100 GAP</atitle><jtitle>Placenta (Eastbourne)</jtitle><addtitle>Placenta</addtitle><date>1998-03-01</date><risdate>1998</risdate><volume>19</volume><issue>2</issue><spage>225</spage><epage>230</epage><pages>225-230</pages><issn>0143-4004</issn><eissn>1532-3102</eissn><coden>PLACDF</coden><abstract>This study was based on the hypothesis that both primary sequence and methylation status of the GTPase activating protein (GAP) gene limits expression of p100 GAP to primate placenta. Due to alternate splicing, a 65-bp insert appears between the first and second coding exons of p100 GAP mRNA, and translation of p100 GAP initiates within this insert. Examination of the sequence surrounding the 65-bp insert revealed that the monkey GAP gene contained both the 3′ splice donor site and the internal start codon, whereas the mouse GAP gene contained neither. To address p100 GAP tissue specificity, the methylation status of the GAP gene was examined. Site-specific demethylation was found to correlate with synthesis of p100 GAP, suggesting that methylation regulates the expression of different GAP isoforms. The results of this study provide a mechanistic basis for the observation that p100 GAP is synthesized only in primate placenta and suggest that its expression is regulated, in part, by gene methylation.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>9548190</pmid><doi>10.1016/S0143-4004(98)90012-1</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adult Animals Base Sequence Biological and medical sciences DNA - analysis DNA Methylation Embryology: invertebrates and vertebrates. Teratology Female Fetal membranes Fibroblasts - enzymology Fundamental and applied biological sciences. Psychology General aspects. Development. Fetal membranes GTP Phosphohydrolases - genetics GTP Phosphohydrolases - metabolism GTPase-Activating Proteins Haplorhini Humans Male Mice Molecular Sequence Data Placenta - enzymology Polymerase Chain Reaction Pregnancy Proteins - genetics Proteins - metabolism Rats Rats, Sprague-Dawley Sequence Homology, Nucleic Acid Species Specificity Tumor Cells, Cultured - enzymology |
| title | Determinants of species- and placenta-specific expression of p100 GAP |
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