Characterization of Interpolyelectrolyte Complexes between Double-Stranded DNA and Polylysine Comb-Type Copolymers Having Hydrophilic Side Chains
The polyionic interaction between DNA and polycations grafted with hydrophilic dextran side chains was evaluated. The comb-type copolymers, poly(l-lysine)-graft-dextran, were successfully prepared by employing a reductive amination reaction between ε-amino groups of poly(l-lysine) (PLL) and the redu...
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Veröffentlicht in: | Bioconjugate chemistry 1998-03, Vol.9 (2), p.292-299 |
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description | The polyionic interaction between DNA and polycations grafted with hydrophilic dextran side chains was evaluated. The comb-type copolymers, poly(l-lysine)-graft-dextran, were successfully prepared by employing a reductive amination reaction between ε-amino groups of poly(l-lysine) (PLL) and the reductive ends of dextran (Dex). A coupling efficacy on the order of 70% was obtained regardless of intrinsic philicities of the solvents used, either aqueous buffer or DMSO. The resulting graft copolymers, which varied in the degree of grafting and the length of hydrophilic side chains, formed a soluble complex with DNA. They also affected the melting behavior of double-stranded DNA (dsDNA) in different ways. Copolymers having a high degree of grafting thermally stabilized dsDNA without affecting its reversible transition between single-stranded and double-stranded forms. However, copolymers with a low degree of grafting or with a high degree of grafting of short dextran chains impeded the reversibility of this transistion. Furthermore, highly grafted copolymers also accelerated the hybridization of DNA strands in a low-ionic strength medium. It is of particular note that these copolymers scarcely altered circular dichroismic signals of dsDNA even when the copolymers were added in excess. This suggested that the copolymer interacted with dsDNA without affecting its native structure or physicochemical properties. Finally, the copolymer even formed a stable complex with a short oligonucleotide (20 bases). We, therefore, concluded that, by regulating the degree of grafting and the molecular weight of grafted side chains, it would be possible to design novel different graft copolymers capable of acting as carriers of functional genes to target cells or tissue. |
doi_str_mv | 10.1021/bc9701510 |
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The comb-type copolymers, poly(l-lysine)-graft-dextran, were successfully prepared by employing a reductive amination reaction between ε-amino groups of poly(l-lysine) (PLL) and the reductive ends of dextran (Dex). A coupling efficacy on the order of 70% was obtained regardless of intrinsic philicities of the solvents used, either aqueous buffer or DMSO. The resulting graft copolymers, which varied in the degree of grafting and the length of hydrophilic side chains, formed a soluble complex with DNA. They also affected the melting behavior of double-stranded DNA (dsDNA) in different ways. Copolymers having a high degree of grafting thermally stabilized dsDNA without affecting its reversible transition between single-stranded and double-stranded forms. However, copolymers with a low degree of grafting or with a high degree of grafting of short dextran chains impeded the reversibility of this transistion. Furthermore, highly grafted copolymers also accelerated the hybridization of DNA strands in a low-ionic strength medium. It is of particular note that these copolymers scarcely altered circular dichroismic signals of dsDNA even when the copolymers were added in excess. This suggested that the copolymer interacted with dsDNA without affecting its native structure or physicochemical properties. Finally, the copolymer even formed a stable complex with a short oligonucleotide (20 bases). We, therefore, concluded that, by regulating the degree of grafting and the molecular weight of grafted side chains, it would be possible to design novel different graft copolymers capable of acting as carriers of functional genes to target cells or tissue.</description><identifier>ISSN: 1043-1802</identifier><identifier>EISSN: 1520-4812</identifier><identifier>DOI: 10.1021/bc9701510</identifier><identifier>PMID: 9548547</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Cations ; Cattle ; Chemical Phenomena ; Chemistry, Physical ; Circular Dichroism ; Dextrans - chemistry ; Dimethyl Sulfoxide ; DNA - chemistry ; Drug Stability ; Electrochemistry ; Electrophoresis, Agar Gel ; Hot Temperature ; Molecular Weight ; Nephelometry and Turbidimetry ; Nucleic Acid Conformation ; Nucleic Acid Hybridization ; Osmolar Concentration ; Poly dA-dT - chemistry ; Polylysine - chemistry ; Solubility ; Water</subject><ispartof>Bioconjugate chemistry, 1998-03, Vol.9 (2), p.292-299</ispartof><rights>Copyright © 1998 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a458t-f84f966a294c12c65dd2ee46c838f5c030d74dfcd2afd6a5968643180e4bf9f73</citedby><cites>FETCH-LOGICAL-a458t-f84f966a294c12c65dd2ee46c838f5c030d74dfcd2afd6a5968643180e4bf9f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bc9701510$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bc9701510$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9548547$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maruyama, Atsushi</creatorcontrib><creatorcontrib>Watanabe, Hiromitsu</creatorcontrib><creatorcontrib>Ferdous, Anwarul</creatorcontrib><creatorcontrib>Katoh, Maiko</creatorcontrib><creatorcontrib>Ishihara, Tsutomu</creatorcontrib><creatorcontrib>Akaike, Toshihiro</creatorcontrib><title>Characterization of Interpolyelectrolyte Complexes between Double-Stranded DNA and Polylysine Comb-Type Copolymers Having Hydrophilic Side Chains</title><title>Bioconjugate chemistry</title><addtitle>Bioconjugate Chem</addtitle><description>The polyionic interaction between DNA and polycations grafted with hydrophilic dextran side chains was evaluated. The comb-type copolymers, poly(l-lysine)-graft-dextran, were successfully prepared by employing a reductive amination reaction between ε-amino groups of poly(l-lysine) (PLL) and the reductive ends of dextran (Dex). A coupling efficacy on the order of 70% was obtained regardless of intrinsic philicities of the solvents used, either aqueous buffer or DMSO. The resulting graft copolymers, which varied in the degree of grafting and the length of hydrophilic side chains, formed a soluble complex with DNA. They also affected the melting behavior of double-stranded DNA (dsDNA) in different ways. Copolymers having a high degree of grafting thermally stabilized dsDNA without affecting its reversible transition between single-stranded and double-stranded forms. However, copolymers with a low degree of grafting or with a high degree of grafting of short dextran chains impeded the reversibility of this transistion. Furthermore, highly grafted copolymers also accelerated the hybridization of DNA strands in a low-ionic strength medium. It is of particular note that these copolymers scarcely altered circular dichroismic signals of dsDNA even when the copolymers were added in excess. This suggested that the copolymer interacted with dsDNA without affecting its native structure or physicochemical properties. Finally, the copolymer even formed a stable complex with a short oligonucleotide (20 bases). We, therefore, concluded that, by regulating the degree of grafting and the molecular weight of grafted side chains, it would be possible to design novel different graft copolymers capable of acting as carriers of functional genes to target cells or tissue.</description><subject>Animals</subject><subject>Cations</subject><subject>Cattle</subject><subject>Chemical Phenomena</subject><subject>Chemistry, Physical</subject><subject>Circular Dichroism</subject><subject>Dextrans - chemistry</subject><subject>Dimethyl Sulfoxide</subject><subject>DNA - chemistry</subject><subject>Drug Stability</subject><subject>Electrochemistry</subject><subject>Electrophoresis, Agar Gel</subject><subject>Hot Temperature</subject><subject>Molecular Weight</subject><subject>Nephelometry and Turbidimetry</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic Acid Hybridization</subject><subject>Osmolar Concentration</subject><subject>Poly dA-dT - chemistry</subject><subject>Polylysine - chemistry</subject><subject>Solubility</subject><subject>Water</subject><issn>1043-1802</issn><issn>1520-4812</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkEFv1DAQhSMEKqVw4Acg-QISh4Dt2El8rHaBrWhLpV2KxMVy7DHrksTBTqDhX_CP8bKrPXGaN3rfvJFelj0n-A3BlLxttKgw4QQ_yE4JpzhnNaEPk8asyEmN6ePsSYx3GGNBanqSnQjOas6q0-zPYquC0iME91uNzvfIW3TRp33w7Qwt6DEkMQJa-G5o4R4iamD8BdCjpZ-aFvL1GFRvwKDl9TlKCt2kg3aOrv931OSbedipXWAHIaKV-un6b2g1m-CHrWudRmtnErJVro9Ps0dWtRGeHeZZ9vn9u81ilV9--nCxOL_MFeP1mNuaWVGWigqmCdUlN4YCsFLXRW25xgU2FTNWG6qsKRUXZV2yInUBrLHCVsVZ9mqfOwT_Y4I4ys5FDW2revBTlJWoBKeCJPD1HtTBxxjAyiG4ToVZEix39ctj_Yl9cQidmg7MkTz0nfx877s4wv3RVuG7LKui4nJzs5a3X65vr5ZXH-XXxL_c80pHeeen0KdK_vP3L5slndA</recordid><startdate>19980301</startdate><enddate>19980301</enddate><creator>Maruyama, Atsushi</creator><creator>Watanabe, Hiromitsu</creator><creator>Ferdous, Anwarul</creator><creator>Katoh, Maiko</creator><creator>Ishihara, Tsutomu</creator><creator>Akaike, Toshihiro</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980301</creationdate><title>Characterization of Interpolyelectrolyte Complexes between Double-Stranded DNA and Polylysine Comb-Type Copolymers Having Hydrophilic Side Chains</title><author>Maruyama, Atsushi ; Watanabe, Hiromitsu ; Ferdous, Anwarul ; Katoh, Maiko ; Ishihara, Tsutomu ; Akaike, Toshihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a458t-f84f966a294c12c65dd2ee46c838f5c030d74dfcd2afd6a5968643180e4bf9f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Cations</topic><topic>Cattle</topic><topic>Chemical Phenomena</topic><topic>Chemistry, Physical</topic><topic>Circular Dichroism</topic><topic>Dextrans - chemistry</topic><topic>Dimethyl Sulfoxide</topic><topic>DNA - chemistry</topic><topic>Drug Stability</topic><topic>Electrochemistry</topic><topic>Electrophoresis, Agar Gel</topic><topic>Hot Temperature</topic><topic>Molecular Weight</topic><topic>Nephelometry and Turbidimetry</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleic Acid Hybridization</topic><topic>Osmolar Concentration</topic><topic>Poly dA-dT - chemistry</topic><topic>Polylysine - chemistry</topic><topic>Solubility</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maruyama, Atsushi</creatorcontrib><creatorcontrib>Watanabe, Hiromitsu</creatorcontrib><creatorcontrib>Ferdous, Anwarul</creatorcontrib><creatorcontrib>Katoh, Maiko</creatorcontrib><creatorcontrib>Ishihara, Tsutomu</creatorcontrib><creatorcontrib>Akaike, Toshihiro</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioconjugate chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maruyama, Atsushi</au><au>Watanabe, Hiromitsu</au><au>Ferdous, Anwarul</au><au>Katoh, Maiko</au><au>Ishihara, Tsutomu</au><au>Akaike, Toshihiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Interpolyelectrolyte Complexes between Double-Stranded DNA and Polylysine Comb-Type Copolymers Having Hydrophilic Side Chains</atitle><jtitle>Bioconjugate chemistry</jtitle><addtitle>Bioconjugate Chem</addtitle><date>1998-03-01</date><risdate>1998</risdate><volume>9</volume><issue>2</issue><spage>292</spage><epage>299</epage><pages>292-299</pages><issn>1043-1802</issn><eissn>1520-4812</eissn><abstract>The polyionic interaction between DNA and polycations grafted with hydrophilic dextran side chains was evaluated. The comb-type copolymers, poly(l-lysine)-graft-dextran, were successfully prepared by employing a reductive amination reaction between ε-amino groups of poly(l-lysine) (PLL) and the reductive ends of dextran (Dex). A coupling efficacy on the order of 70% was obtained regardless of intrinsic philicities of the solvents used, either aqueous buffer or DMSO. The resulting graft copolymers, which varied in the degree of grafting and the length of hydrophilic side chains, formed a soluble complex with DNA. They also affected the melting behavior of double-stranded DNA (dsDNA) in different ways. Copolymers having a high degree of grafting thermally stabilized dsDNA without affecting its reversible transition between single-stranded and double-stranded forms. However, copolymers with a low degree of grafting or with a high degree of grafting of short dextran chains impeded the reversibility of this transistion. Furthermore, highly grafted copolymers also accelerated the hybridization of DNA strands in a low-ionic strength medium. It is of particular note that these copolymers scarcely altered circular dichroismic signals of dsDNA even when the copolymers were added in excess. This suggested that the copolymer interacted with dsDNA without affecting its native structure or physicochemical properties. Finally, the copolymer even formed a stable complex with a short oligonucleotide (20 bases). We, therefore, concluded that, by regulating the degree of grafting and the molecular weight of grafted side chains, it would be possible to design novel different graft copolymers capable of acting as carriers of functional genes to target cells or tissue.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>9548547</pmid><doi>10.1021/bc9701510</doi><tpages>8</tpages></addata></record> |
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subjects | Animals Cations Cattle Chemical Phenomena Chemistry, Physical Circular Dichroism Dextrans - chemistry Dimethyl Sulfoxide DNA - chemistry Drug Stability Electrochemistry Electrophoresis, Agar Gel Hot Temperature Molecular Weight Nephelometry and Turbidimetry Nucleic Acid Conformation Nucleic Acid Hybridization Osmolar Concentration Poly dA-dT - chemistry Polylysine - chemistry Solubility Water |
title | Characterization of Interpolyelectrolyte Complexes between Double-Stranded DNA and Polylysine Comb-Type Copolymers Having Hydrophilic Side Chains |
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