Detection of human papillomavirus dna in laryngeal squamous cell carcinomas by polymerase chain reaction
The presence of human papillomavirus genomes‐16 and ‐6b in metastatic cervical lymph nodes was examined in 34 cases of laryngeal carcinomas by means of polymerase chain reaction, which had been fixed in formalin and embedded in paraffin. Human papillomavirus DNAs extracted from paraffin‐embedded tum...
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| Veröffentlicht in: | The Laryngoscope 1990-06, Vol.100 (6), p.647-650 |
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| container_title | The Laryngoscope |
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| creator | Hoshikawa, Tomohide Nakajima, Takashi Uhara, Hisashi Gotoh, Masahiro Shimosato, Yukio Tsutsumi, Kouichiro Ono, Isamu Ebihara, Satoshi |
| description | The presence of human papillomavirus genomes‐16 and ‐6b in metastatic cervical lymph nodes was examined in 34 cases of laryngeal carcinomas by means of polymerase chain reaction, which had been fixed in formalin and embedded in paraffin. Human papillomavirus DNAs extracted from paraffin‐embedded tumor tissues were used for polymerase chain reaction with amplification of the E6 region of human papillomavirus genome‐16 and the El region of human papillomavirus genome‐6b. Human papillomavirus genome‐16 sequences were positively amplified in six (17.6%) metastatic tumors; ‐6b sequence was positively amplified in one (2.9%) metastatic tumor. Laryngeal carcinomas of glottic origin showed high human papillomavirus genome‐16 DNA‐positive rates (4 of 9 cases, 44.4%) compared to those of other sites. These results suggest that human papillomavirus genome‐16 infection might be closely associated with the development of some laryngeal squamous cell carcinomas of glottic origin similar to uterine cervical carcino‐genesis. |
| doi_str_mv | 10.1288/00005537-199006000-00018 |
| format | Article |
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Human papillomavirus DNAs extracted from paraffin‐embedded tumor tissues were used for polymerase chain reaction with amplification of the E6 region of human papillomavirus genome‐16 and the El region of human papillomavirus genome‐6b. Human papillomavirus genome‐16 sequences were positively amplified in six (17.6%) metastatic tumors; ‐6b sequence was positively amplified in one (2.9%) metastatic tumor. Laryngeal carcinomas of glottic origin showed high human papillomavirus genome‐16 DNA‐positive rates (4 of 9 cases, 44.4%) compared to those of other sites. These results suggest that human papillomavirus genome‐16 infection might be closely associated with the development of some laryngeal squamous cell carcinomas of glottic origin similar to uterine cervical carcino‐genesis.</description><identifier>ISSN: 0023-852X</identifier><identifier>EISSN: 1531-4995</identifier><identifier>DOI: 10.1288/00005537-199006000-00018</identifier><identifier>PMID: 2348745</identifier><identifier>CODEN: LARYA8</identifier><language>eng</language><publisher>Hoboken, NJ: John Wiley & Sons, Inc</publisher><subject>Biological and medical sciences ; Carcinoma, Squamous Cell - microbiology ; Carcinoma, Squamous Cell - pathology ; DNA Probes - analysis ; DNA Probes, HPV - analysis ; Female ; Gene Amplification ; Humans ; Laryngeal Neoplasms - microbiology ; Laryngeal Neoplasms - pathology ; Lymphatic Metastasis ; Male ; Medical sciences ; Middle Aged ; Otorhinolaryngology. Stomatology ; Polymerase Chain Reaction ; Tumors ; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><ispartof>The Laryngoscope, 1990-06, Vol.100 (6), p.647-650</ispartof><rights>Copyright © 1990 The Triological Society</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5058-d92af9bab7fc73e3d31ce2ac9d755df65ffeed261b1e738a5697b32d1f98c2ac3</citedby><cites>FETCH-LOGICAL-c5058-d92af9bab7fc73e3d31ce2ac9d755df65ffeed261b1e738a5697b32d1f98c2ac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19837526$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2348745$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hoshikawa, Tomohide</creatorcontrib><creatorcontrib>Nakajima, Takashi</creatorcontrib><creatorcontrib>Uhara, Hisashi</creatorcontrib><creatorcontrib>Gotoh, Masahiro</creatorcontrib><creatorcontrib>Shimosato, Yukio</creatorcontrib><creatorcontrib>Tsutsumi, Kouichiro</creatorcontrib><creatorcontrib>Ono, Isamu</creatorcontrib><creatorcontrib>Ebihara, Satoshi</creatorcontrib><title>Detection of human papillomavirus dna in laryngeal squamous cell carcinomas by polymerase chain reaction</title><title>The Laryngoscope</title><addtitle>The Laryngoscope</addtitle><description>The presence of human papillomavirus genomes‐16 and ‐6b in metastatic cervical lymph nodes was examined in 34 cases of laryngeal carcinomas by means of polymerase chain reaction, which had been fixed in formalin and embedded in paraffin. Human papillomavirus DNAs extracted from paraffin‐embedded tumor tissues were used for polymerase chain reaction with amplification of the E6 region of human papillomavirus genome‐16 and the El region of human papillomavirus genome‐6b. Human papillomavirus genome‐16 sequences were positively amplified in six (17.6%) metastatic tumors; ‐6b sequence was positively amplified in one (2.9%) metastatic tumor. Laryngeal carcinomas of glottic origin showed high human papillomavirus genome‐16 DNA‐positive rates (4 of 9 cases, 44.4%) compared to those of other sites. These results suggest that human papillomavirus genome‐16 infection might be closely associated with the development of some laryngeal squamous cell carcinomas of glottic origin similar to uterine cervical carcino‐genesis.</description><subject>Biological and medical sciences</subject><subject>Carcinoma, Squamous Cell - microbiology</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>DNA Probes - analysis</subject><subject>DNA Probes, HPV - analysis</subject><subject>Female</subject><subject>Gene Amplification</subject><subject>Humans</subject><subject>Laryngeal Neoplasms - microbiology</subject><subject>Laryngeal Neoplasms - pathology</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Otorhinolaryngology. 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| ispartof | The Laryngoscope, 1990-06, Vol.100 (6), p.647-650 |
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| language | eng |
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| subjects | Biological and medical sciences Carcinoma, Squamous Cell - microbiology Carcinoma, Squamous Cell - pathology DNA Probes - analysis DNA Probes, HPV - analysis Female Gene Amplification Humans Laryngeal Neoplasms - microbiology Laryngeal Neoplasms - pathology Lymphatic Metastasis Male Medical sciences Middle Aged Otorhinolaryngology. Stomatology Polymerase Chain Reaction Tumors Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology |
| title | Detection of human papillomavirus dna in laryngeal squamous cell carcinomas by polymerase chain reaction |
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