Expression of verapamil hypersensitivity in multidrug-resistant cells grown as multicellular spheroids
It is known that certain multidrug-resistant cell lines are hypersensitive to verapamil and to some other membrane-active agents. We examined the expression of verapamil hypersensitivity in multidrug-resistant, verapamil-hypersensitive CHO cell line by cells grown in the form of multicellular sphero...
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| Veröffentlicht in: | Cancer chemotherapy and pharmacology 1990-01, Vol.26 (2), p.151-154 |
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| container_title | Cancer chemotherapy and pharmacology |
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| creator | ANDERSON, M WARR, J. R |
| description | It is known that certain multidrug-resistant cell lines are hypersensitive to verapamil and to some other membrane-active agents. We examined the expression of verapamil hypersensitivity in multidrug-resistant, verapamil-hypersensitive CHO cell line by cells grown in the form of multicellular spheroids. We found that although vincristine resistance is expressed at high levels in multicellular spheroids, we could not detect verapamil hypersensitivity when cells were grown in this form. Using a dry autoradiographic method to study the entry of [3H]-verapamil into the spheroids, we demonstrated that the lack of hypersensitivity was not due to incomplete drug penetration. We therefore propose that when hypersensitive multidrug-resistant cells are grown as three-dimensional spheroids, cellular interactions may modify the expression of verapamil hypersensitivity. |
| doi_str_mv | 10.1007/BF02897263 |
| format | Article |
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We therefore propose that when hypersensitive multidrug-resistant cells are grown as three-dimensional spheroids, cellular interactions may modify the expression of verapamil hypersensitivity.</description><identifier>ISSN: 0344-5704</identifier><identifier>EISSN: 1432-0843</identifier><identifier>DOI: 10.1007/BF02897263</identifier><identifier>PMID: 2347041</identifier><identifier>CODEN: CCPHDZ</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Biological and medical sciences ; Cardiovascular system ; Cattle ; Cell Division ; Cricetinae ; Drug Hypersensitivity - genetics ; Drug Resistance ; Female ; Medical sciences ; Miscellaneous ; Pharmacology. 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We therefore propose that when hypersensitive multidrug-resistant cells are grown as three-dimensional spheroids, cellular interactions may modify the expression of verapamil hypersensitivity.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cardiovascular system</subject><subject>Cattle</subject><subject>Cell Division</subject><subject>Cricetinae</subject><subject>Drug Hypersensitivity - genetics</subject><subject>Drug Resistance</subject><subject>Female</subject><subject>Medical sciences</subject><subject>Miscellaneous</subject><subject>Pharmacology. 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Using a dry autoradiographic method to study the entry of [3H]-verapamil into the spheroids, we demonstrated that the lack of hypersensitivity was not due to incomplete drug penetration. We therefore propose that when hypersensitive multidrug-resistant cells are grown as three-dimensional spheroids, cellular interactions may modify the expression of verapamil hypersensitivity.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>2347041</pmid><doi>10.1007/BF02897263</doi><tpages>4</tpages></addata></record> |
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| ispartof | Cancer chemotherapy and pharmacology, 1990-01, Vol.26 (2), p.151-154 |
| issn | 0344-5704 1432-0843 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biological and medical sciences Cardiovascular system Cattle Cell Division Cricetinae Drug Hypersensitivity - genetics Drug Resistance Female Medical sciences Miscellaneous Pharmacology. Drug treatments Tumor Cells, Cultured Verapamil - immunology Vincristine - pharmacology |
| title | Expression of verapamil hypersensitivity in multidrug-resistant cells grown as multicellular spheroids |
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