Immunohistochemical Evidence for IgE Involvement in Graves' Orbitopathy
Orbital muscle and adipose tissues from seven Graves' orbitopathy patients were studied with in situ assays for IgE. The cases varied in disease severity and site biopsied. Two monoclonal and one polyclonal anti-IgE reagents produced similar results. Identically prepared monoclonal anti-IgM and...
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| Veröffentlicht in: | Ophthalmology (Rochester, Minn.) Minn.), 1990-05, Vol.97 (5), p.629-635 |
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| container_title | Ophthalmology (Rochester, Minn.) |
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| creator | Raikow, Radmila B. Dalbow, Milton H. Kennerdell, John S. Compher, Keen Machen, Laurie Hiller, William Blendermann, Debbie |
| description | Orbital muscle and adipose tissues from seven Graves' orbitopathy patients were studied with in situ assays for IgE. The cases varied in disease severity and site biopsied. Two monoclonal and one polyclonal anti-IgE reagents produced similar results. Identically prepared monoclonal anti-IgM and anti-IgG antibodies and tissues obtained from five patients treated for unrelated orbital disorders were used as controls. Graves' tissues exhibited extravasated leukocytes and leukocyte-rich vessels. These leukocytes were mostly lymphoid. Some basophils and mast cells were identified and polymorphonuclear cells were abundant within vessels of adipose tissue. IgE-positive material was found in association with the majority of leukocytes and with muscle fibers. Parallel sections reacted with anti-IgM antibody were negative, whereas anti-IgG produced diffuse staining with no specific structures highlighted. Control, non-Graves' tissues had no evidence of immune cell activity and were either negative or displayed reactions with anti-IgE reagents that were in most cases different from those of Graves' tissue. Serum IgE was measured in six of the seven patients and was elevated in the two patients with fulminating disease. |
| doi_str_mv | 10.1016/S0161-6420(90)32548-4 |
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The cases varied in disease severity and site biopsied. Two monoclonal and one polyclonal anti-IgE reagents produced similar results. Identically prepared monoclonal anti-IgM and anti-IgG antibodies and tissues obtained from five patients treated for unrelated orbital disorders were used as controls. Graves' tissues exhibited extravasated leukocytes and leukocyte-rich vessels. These leukocytes were mostly lymphoid. Some basophils and mast cells were identified and polymorphonuclear cells were abundant within vessels of adipose tissue. IgE-positive material was found in association with the majority of leukocytes and with muscle fibers. Parallel sections reacted with anti-IgM antibody were negative, whereas anti-IgG produced diffuse staining with no specific structures highlighted. Control, non-Graves' tissues had no evidence of immune cell activity and were either negative or displayed reactions with anti-IgE reagents that were in most cases different from those of Graves' tissue. Serum IgE was measured in six of the seven patients and was elevated in the two patients with fulminating disease.</description><identifier>ISSN: 0161-6420</identifier><identifier>EISSN: 1549-4713</identifier><identifier>DOI: 10.1016/S0161-6420(90)32548-4</identifier><identifier>PMID: 2188194</identifier><identifier>CODEN: OPHTDG</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adipose Tissue - immunology ; Aged ; Antibodies, Monoclonal ; Biological and medical sciences ; Endocrinopathies ; Enzyme-Linked Immunosorbent Assay ; Female ; Graves Disease - immunology ; Humans ; Immunoenzyme Techniques ; Immunoglobulin E - analysis ; Immunohistochemistry ; Leukocytes, Mononuclear - immunology ; Lymphocytes - immunology ; Male ; Medical sciences ; Middle Aged ; Non tumoral diseases. Target tissue resistance. Benign neoplasms ; Oculomotor Muscles - immunology ; Thyroid. 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The cases varied in disease severity and site biopsied. Two monoclonal and one polyclonal anti-IgE reagents produced similar results. Identically prepared monoclonal anti-IgM and anti-IgG antibodies and tissues obtained from five patients treated for unrelated orbital disorders were used as controls. Graves' tissues exhibited extravasated leukocytes and leukocyte-rich vessels. These leukocytes were mostly lymphoid. Some basophils and mast cells were identified and polymorphonuclear cells were abundant within vessels of adipose tissue. IgE-positive material was found in association with the majority of leukocytes and with muscle fibers. Parallel sections reacted with anti-IgM antibody were negative, whereas anti-IgG produced diffuse staining with no specific structures highlighted. Control, non-Graves' tissues had no evidence of immune cell activity and were either negative or displayed reactions with anti-IgE reagents that were in most cases different from those of Graves' tissue. Serum IgE was measured in six of the seven patients and was elevated in the two patients with fulminating disease.</description><subject>Adipose Tissue - immunology</subject><subject>Aged</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Endocrinopathies</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Graves Disease - immunology</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Immunoglobulin E - analysis</subject><subject>Immunohistochemistry</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Lymphocytes - immunology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Non tumoral diseases. Target tissue resistance. Benign neoplasms</subject><subject>Oculomotor Muscles - immunology</subject><subject>Thyroid. Thyroid axis (diseases)</subject><issn>0161-6420</issn><issn>1549-4713</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v2zAMhoWhRZd2-wkFfGm3HdxRH46tUzEUaRYgQA7tzoJM04sG20ol20D-_ZwPdMddyAOflyQexm45PHDg8-8vU-HpXAn4quGbFJkqUvWBzXimdKpyLi_Y7B35yK5j_AMA87lUV-xK8KLgWs3YctW2Q-e3LvYet9Q6tE2yGF1FHVJS-5Csfi-SVTf6ZqSWuj5xXbIMdqT4JdmE0vV-Z_vt_hO7rG0T6fO537Bfz4vXp5_perNcPf1Ypyg19CmJDKkC0lrwCgAFR5AlkrK2pIKU1DWUOdTKZlZYLNFmqKGQysqaEHN5w-5Pe3fBvw0Ue9O6iNQ0tiM_RJPrvJCSZxOYnUAMPsZAtdkF19qwNxzMQaA5CjQHO0aDOQo0asrdng8MZUvVe-psbJrfnec2Tq7qYDt08d9yLZQWABP3eOJosjE6CiaiO0itXCDsTeXdfz75C7zcjZ0</recordid><startdate>19900501</startdate><enddate>19900501</enddate><creator>Raikow, Radmila B.</creator><creator>Dalbow, Milton H.</creator><creator>Kennerdell, John S.</creator><creator>Compher, Keen</creator><creator>Machen, Laurie</creator><creator>Hiller, William</creator><creator>Blendermann, Debbie</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900501</creationdate><title>Immunohistochemical Evidence for IgE Involvement in Graves' Orbitopathy</title><author>Raikow, Radmila B. ; Dalbow, Milton H. ; Kennerdell, John S. ; Compher, Keen ; Machen, Laurie ; Hiller, William ; Blendermann, Debbie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-e25ced0e9921d00c21c03bce4aabe8e439f0b70f4a5a2acbca5c90834a3fecc73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Adipose Tissue - immunology</topic><topic>Aged</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Endocrinopathies</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Graves Disease - immunology</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Immunoglobulin E - analysis</topic><topic>Immunohistochemistry</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Lymphocytes - immunology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Non tumoral diseases. Target tissue resistance. Benign neoplasms</topic><topic>Oculomotor Muscles - immunology</topic><topic>Thyroid. Thyroid axis (diseases)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raikow, Radmila B.</creatorcontrib><creatorcontrib>Dalbow, Milton H.</creatorcontrib><creatorcontrib>Kennerdell, John S.</creatorcontrib><creatorcontrib>Compher, Keen</creatorcontrib><creatorcontrib>Machen, Laurie</creatorcontrib><creatorcontrib>Hiller, William</creatorcontrib><creatorcontrib>Blendermann, Debbie</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Ophthalmology (Rochester, Minn.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raikow, Radmila B.</au><au>Dalbow, Milton H.</au><au>Kennerdell, John S.</au><au>Compher, Keen</au><au>Machen, Laurie</au><au>Hiller, William</au><au>Blendermann, Debbie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical Evidence for IgE Involvement in Graves' Orbitopathy</atitle><jtitle>Ophthalmology (Rochester, Minn.)</jtitle><addtitle>Ophthalmology</addtitle><date>1990-05-01</date><risdate>1990</risdate><volume>97</volume><issue>5</issue><spage>629</spage><epage>635</epage><pages>629-635</pages><issn>0161-6420</issn><eissn>1549-4713</eissn><coden>OPHTDG</coden><abstract>Orbital muscle and adipose tissues from seven Graves' orbitopathy patients were studied with in situ assays for IgE. The cases varied in disease severity and site biopsied. Two monoclonal and one polyclonal anti-IgE reagents produced similar results. Identically prepared monoclonal anti-IgM and anti-IgG antibodies and tissues obtained from five patients treated for unrelated orbital disorders were used as controls. Graves' tissues exhibited extravasated leukocytes and leukocyte-rich vessels. These leukocytes were mostly lymphoid. Some basophils and mast cells were identified and polymorphonuclear cells were abundant within vessels of adipose tissue. IgE-positive material was found in association with the majority of leukocytes and with muscle fibers. Parallel sections reacted with anti-IgM antibody were negative, whereas anti-IgG produced diffuse staining with no specific structures highlighted. Control, non-Graves' tissues had no evidence of immune cell activity and were either negative or displayed reactions with anti-IgE reagents that were in most cases different from those of Graves' tissue. Serum IgE was measured in six of the seven patients and was elevated in the two patients with fulminating disease.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>2188194</pmid><doi>10.1016/S0161-6420(90)32548-4</doi><tpages>7</tpages></addata></record> |
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| ispartof | Ophthalmology (Rochester, Minn.), 1990-05, Vol.97 (5), p.629-635 |
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| language | eng |
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| subjects | Adipose Tissue - immunology Aged Antibodies, Monoclonal Biological and medical sciences Endocrinopathies Enzyme-Linked Immunosorbent Assay Female Graves Disease - immunology Humans Immunoenzyme Techniques Immunoglobulin E - analysis Immunohistochemistry Leukocytes, Mononuclear - immunology Lymphocytes - immunology Male Medical sciences Middle Aged Non tumoral diseases. Target tissue resistance. Benign neoplasms Oculomotor Muscles - immunology Thyroid. Thyroid axis (diseases) |
| title | Immunohistochemical Evidence for IgE Involvement in Graves' Orbitopathy |
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