The isolation of osmotic-remedial conditional lethal mutants of Candida albicans
We have developed a method for the isolation of a broad range of conditional lethal mutants of the pathogenic fungus Candida albicans. The method substantially alleviates the problems posed by the diploid nature, and the biased mutant spectrum, exhibited by most strains of this organism. We have use...
Gespeichert in:
| Veröffentlicht in: | Current genetics 1990-04, Vol.17 (4), p.293-296 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 296 |
|---|---|
| container_issue | 4 |
| container_start_page | 293 |
| container_title | Current genetics |
| container_volume | 17 |
| creator | PAYTON, M. A DE TIANI, M |
| description | We have developed a method for the isolation of a broad range of conditional lethal mutants of the pathogenic fungus Candida albicans. The method substantially alleviates the problems posed by the diploid nature, and the biased mutant spectrum, exhibited by most strains of this organism. We have used the method to isolate 560 temperature-sensitive mutants which grew at 30 degrees C but not at 42 degrees C. We identified amongst them a number of osmotic-remedial strains which, at the restrictive temperature, show phenotypes indicating defects in cell wall biosynthesis. |
| doi_str_mv | 10.1007/BF00314875 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79775860</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15768301</sourcerecordid><originalsourceid>FETCH-LOGICAL-c342t-38147970b5f2b86755c4ed17f2d403eb13bd2be6fa6dd0787d7923fe5cf98b6b3</originalsourceid><addsrcrecordid>eNqFkDtPwzAURi0EKqWwsCNlQAxIgWs7sZ0RKl5SJRjKHPmpGjlxiZOBf0-qRmVkulc6R99wELrEcIcB-P3jMwDFheDlEZrjgpIcKkGP0RwwJ7kAQU_RWUpfAJiIis_QjGDBGWFz9LHe2MynGGTvY5tFl8XUxN7rvLONNV6GTMfW-B0d_2D7zXiaoZdtn3b6Uo7UyEwG5bVs0zk6cTIkezHdBfp8flovX_PV-8vb8mGVa1qQPqcCF7zioEpHlGC8LHVhDeaOmAKoVZgqQ5RlTjJjgAtueEWos6V2lVBM0QW62e9uu_g92NTXjU_ahiBbG4dUj-O8FAz-FXHJmaCAR_F2L-ouptRZV28738jup8ZQ7zrXf51H-WpaHdTY6aBOYUd-PXGZtAyuk6326aAxwYEWlP4CfCqELg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15768301</pqid></control><display><type>article</type><title>The isolation of osmotic-remedial conditional lethal mutants of Candida albicans</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>PAYTON, M. A ; DE TIANI, M</creator><creatorcontrib>PAYTON, M. A ; DE TIANI, M</creatorcontrib><description>We have developed a method for the isolation of a broad range of conditional lethal mutants of the pathogenic fungus Candida albicans. The method substantially alleviates the problems posed by the diploid nature, and the biased mutant spectrum, exhibited by most strains of this organism. We have used the method to isolate 560 temperature-sensitive mutants which grew at 30 degrees C but not at 42 degrees C. We identified amongst them a number of osmotic-remedial strains which, at the restrictive temperature, show phenotypes indicating defects in cell wall biosynthesis.</description><identifier>ISSN: 0172-8083</identifier><identifier>EISSN: 1432-0983</identifier><identifier>DOI: 10.1007/BF00314875</identifier><identifier>PMID: 2187626</identifier><identifier>CODEN: CUGED5</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Biological and medical sciences ; Candida albicans - genetics ; Candida albicans - growth & development ; Candida albicans - metabolism ; Cell Wall - metabolism ; Classical genetics, quantitative genetics, hybrids ; Culture Media ; Fundamental and applied biological sciences. Psychology ; Genes, Fungal ; Genes, Lethal ; Genetics of eukaryotes. Biological and molecular evolution ; Mutation ; Osmotic Fragility ; Phenotype ; Temperature ; Thallophyta, bryophyta ; Vegetals</subject><ispartof>Current genetics, 1990-04, Vol.17 (4), p.293-296</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-38147970b5f2b86755c4ed17f2d403eb13bd2be6fa6dd0787d7923fe5cf98b6b3</citedby><cites>FETCH-LOGICAL-c342t-38147970b5f2b86755c4ed17f2d403eb13bd2be6fa6dd0787d7923fe5cf98b6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6870343$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2187626$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PAYTON, M. A</creatorcontrib><creatorcontrib>DE TIANI, M</creatorcontrib><title>The isolation of osmotic-remedial conditional lethal mutants of Candida albicans</title><title>Current genetics</title><addtitle>Curr Genet</addtitle><description>We have developed a method for the isolation of a broad range of conditional lethal mutants of the pathogenic fungus Candida albicans. The method substantially alleviates the problems posed by the diploid nature, and the biased mutant spectrum, exhibited by most strains of this organism. We have used the method to isolate 560 temperature-sensitive mutants which grew at 30 degrees C but not at 42 degrees C. We identified amongst them a number of osmotic-remedial strains which, at the restrictive temperature, show phenotypes indicating defects in cell wall biosynthesis.</description><subject>Biological and medical sciences</subject><subject>Candida albicans - genetics</subject><subject>Candida albicans - growth & development</subject><subject>Candida albicans - metabolism</subject><subject>Cell Wall - metabolism</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Culture Media</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Fungal</subject><subject>Genes, Lethal</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Mutation</subject><subject>Osmotic Fragility</subject><subject>Phenotype</subject><subject>Temperature</subject><subject>Thallophyta, bryophyta</subject><subject>Vegetals</subject><issn>0172-8083</issn><issn>1432-0983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAURi0EKqWwsCNlQAxIgWs7sZ0RKl5SJRjKHPmpGjlxiZOBf0-qRmVkulc6R99wELrEcIcB-P3jMwDFheDlEZrjgpIcKkGP0RwwJ7kAQU_RWUpfAJiIis_QjGDBGWFz9LHe2MynGGTvY5tFl8XUxN7rvLONNV6GTMfW-B0d_2D7zXiaoZdtn3b6Uo7UyEwG5bVs0zk6cTIkezHdBfp8flovX_PV-8vb8mGVa1qQPqcCF7zioEpHlGC8LHVhDeaOmAKoVZgqQ5RlTjJjgAtueEWos6V2lVBM0QW62e9uu_g92NTXjU_ahiBbG4dUj-O8FAz-FXHJmaCAR_F2L-ouptRZV28738jup8ZQ7zrXf51H-WpaHdTY6aBOYUd-PXGZtAyuk6326aAxwYEWlP4CfCqELg</recordid><startdate>19900401</startdate><enddate>19900401</enddate><creator>PAYTON, M. A</creator><creator>DE TIANI, M</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19900401</creationdate><title>The isolation of osmotic-remedial conditional lethal mutants of Candida albicans</title><author>PAYTON, M. A ; DE TIANI, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-38147970b5f2b86755c4ed17f2d403eb13bd2be6fa6dd0787d7923fe5cf98b6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Biological and medical sciences</topic><topic>Candida albicans - genetics</topic><topic>Candida albicans - growth & development</topic><topic>Candida albicans - metabolism</topic><topic>Cell Wall - metabolism</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Culture Media</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Fungal</topic><topic>Genes, Lethal</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Mutation</topic><topic>Osmotic Fragility</topic><topic>Phenotype</topic><topic>Temperature</topic><topic>Thallophyta, bryophyta</topic><topic>Vegetals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PAYTON, M. A</creatorcontrib><creatorcontrib>DE TIANI, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PAYTON, M. A</au><au>DE TIANI, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The isolation of osmotic-remedial conditional lethal mutants of Candida albicans</atitle><jtitle>Current genetics</jtitle><addtitle>Curr Genet</addtitle><date>1990-04-01</date><risdate>1990</risdate><volume>17</volume><issue>4</issue><spage>293</spage><epage>296</epage><pages>293-296</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><coden>CUGED5</coden><abstract>We have developed a method for the isolation of a broad range of conditional lethal mutants of the pathogenic fungus Candida albicans. The method substantially alleviates the problems posed by the diploid nature, and the biased mutant spectrum, exhibited by most strains of this organism. We have used the method to isolate 560 temperature-sensitive mutants which grew at 30 degrees C but not at 42 degrees C. We identified amongst them a number of osmotic-remedial strains which, at the restrictive temperature, show phenotypes indicating defects in cell wall biosynthesis.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>2187626</pmid><doi>10.1007/BF00314875</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0172-8083 |
| ispartof | Current genetics, 1990-04, Vol.17 (4), p.293-296 |
| issn | 0172-8083 1432-0983 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79775860 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Biological and medical sciences Candida albicans - genetics Candida albicans - growth & development Candida albicans - metabolism Cell Wall - metabolism Classical genetics, quantitative genetics, hybrids Culture Media Fundamental and applied biological sciences. Psychology Genes, Fungal Genes, Lethal Genetics of eukaryotes. Biological and molecular evolution Mutation Osmotic Fragility Phenotype Temperature Thallophyta, bryophyta Vegetals |
| title | The isolation of osmotic-remedial conditional lethal mutants of Candida albicans |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T14%3A43%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20isolation%20of%20osmotic-remedial%20conditional%20lethal%20mutants%20of%20Candida%20albicans&rft.jtitle=Current%20genetics&rft.au=PAYTON,%20M.%20A&rft.date=1990-04-01&rft.volume=17&rft.issue=4&rft.spage=293&rft.epage=296&rft.pages=293-296&rft.issn=0172-8083&rft.eissn=1432-0983&rft.coden=CUGED5&rft_id=info:doi/10.1007/BF00314875&rft_dat=%3Cproquest_cross%3E15768301%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15768301&rft_id=info:pmid/2187626&rfr_iscdi=true |