A Single Dose of Antenatal Betamethasone Enhances Isoprenaline and Prostaglandin E2-Induced Relaxation of Preterm Ovine Pulmonary Arteries
β-Adrenergic agonists and prostaglandin E 2 (PGE 2 ) play an important role in perinatal pulmonary circulation. We have determined the effect of antenatal glucocorticoid treatment on isoprenaline- and PGE 2 -mediated relaxation of pulmonary arteries of newborn preterm lambs. Ovine fetuses (121 days...
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| Veröffentlicht in: | Biology of the neonate 1998-03, Vol.73 (3), p.182-189 |
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| creator | Gao, Yuansheng Tolsa, Jean-Francois Shen, Hai Raj, J.Usha |
| description | β-Adrenergic agonists and prostaglandin E 2 (PGE 2 ) play an important role in perinatal pulmonary circulation. We have determined the effect of antenatal glucocorticoid treatment on isoprenaline- and PGE 2 -mediated relaxation of pulmonary arteries of newborn preterm lambs. Ovine fetuses (121 days of gestation; term = 150 days) received a single intramuscular dose of betamethasone (0.5 mg/kg) or saline. Fifteen hours after the injection, the lambs were delivered, ventilated for 3 h, and sacrificed. The fourth-generation pulmonary arteries were dissected and cut into rings for study. In endothelin-1-preconstricted vessels, isoprenaline, PGE 2 , and forskolin (an activator of adenylyl cyclase) induced greater relaxations of pulmonary arteries of betamethasone-treated lambs than those of controls. 8-Bromo-cyclic adenosine monophosphate, a cell membrane permeable analogue of cyclic adenosine monophosphate, caused similar relaxation of all vessels. When stimulated with isoprenaline and PGE 2 , the adenylyl cyclase activity of crude membrane preparations of pulmonary arteries treated with betamethasone was greater than that of controls. These results show that single-dose antenatal betamethasone treatment enhances relaxation of pulmonary arteries of preterm lambs induced by isoprenaline and PGE 2 and that an enhanced adenylyl cyclase activity contributes to the effect of betamethasone on pulmonary arteries of preterm lambs. |
| doi_str_mv | 10.1159/000013976 |
| format | Article |
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We have determined the effect of antenatal glucocorticoid treatment on isoprenaline- and PGE 2 -mediated relaxation of pulmonary arteries of newborn preterm lambs. Ovine fetuses (121 days of gestation; term = 150 days) received a single intramuscular dose of betamethasone (0.5 mg/kg) or saline. Fifteen hours after the injection, the lambs were delivered, ventilated for 3 h, and sacrificed. The fourth-generation pulmonary arteries were dissected and cut into rings for study. In endothelin-1-preconstricted vessels, isoprenaline, PGE 2 , and forskolin (an activator of adenylyl cyclase) induced greater relaxations of pulmonary arteries of betamethasone-treated lambs than those of controls. 8-Bromo-cyclic adenosine monophosphate, a cell membrane permeable analogue of cyclic adenosine monophosphate, caused similar relaxation of all vessels. When stimulated with isoprenaline and PGE 2 , the adenylyl cyclase activity of crude membrane preparations of pulmonary arteries treated with betamethasone was greater than that of controls. These results show that single-dose antenatal betamethasone treatment enhances relaxation of pulmonary arteries of preterm lambs induced by isoprenaline and PGE 2 and that an enhanced adenylyl cyclase activity contributes to the effect of betamethasone on pulmonary arteries of preterm lambs.</description><identifier>ISSN: 1661-7800</identifier><identifier>ISSN: 0006-3126</identifier><identifier>EISSN: 1661-7819</identifier><identifier>EISSN: 1421-9727</identifier><identifier>DOI: 10.1159/000013976</identifier><identifier>PMID: 9535536</identifier><identifier>CODEN: BNEOBV</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Adenylyl Cyclases - metabolism ; Anesthesia. Intensive care medicine. Transfusions. 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We have determined the effect of antenatal glucocorticoid treatment on isoprenaline- and PGE 2 -mediated relaxation of pulmonary arteries of newborn preterm lambs. Ovine fetuses (121 days of gestation; term = 150 days) received a single intramuscular dose of betamethasone (0.5 mg/kg) or saline. Fifteen hours after the injection, the lambs were delivered, ventilated for 3 h, and sacrificed. The fourth-generation pulmonary arteries were dissected and cut into rings for study. In endothelin-1-preconstricted vessels, isoprenaline, PGE 2 , and forskolin (an activator of adenylyl cyclase) induced greater relaxations of pulmonary arteries of betamethasone-treated lambs than those of controls. 8-Bromo-cyclic adenosine monophosphate, a cell membrane permeable analogue of cyclic adenosine monophosphate, caused similar relaxation of all vessels. When stimulated with isoprenaline and PGE 2 , the adenylyl cyclase activity of crude membrane preparations of pulmonary arteries treated with betamethasone was greater than that of controls. These results show that single-dose antenatal betamethasone treatment enhances relaxation of pulmonary arteries of preterm lambs induced by isoprenaline and PGE 2 and that an enhanced adenylyl cyclase activity contributes to the effect of betamethasone on pulmonary arteries of preterm lambs.</description><subject>Adenylyl Cyclases - metabolism</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Betamethasone - administration & dosage</subject><subject>Betamethasone - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Bronchodilator Agents - pharmacology</subject><subject>Dinoprostone - pharmacology</subject><subject>Emergency and intensive care: neonates and children. 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Sudden death</subject><subject>Female</subject><subject>Intensive care medicine</subject><subject>Isoproterenol - pharmacology</subject><subject>Medical sciences</subject><subject>Original Paper</subject><subject>Pregnancy</subject><subject>Pulmonary Artery - embryology</subject><subject>Pulmonary Artery - physiology</subject><subject>Sheep</subject><subject>Vasodilation - drug effects</subject><subject>Vasodilator Agents - pharmacology</subject><issn>1661-7800</issn><issn>0006-3126</issn><issn>1661-7819</issn><issn>1421-9727</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptkU2P0zAQhi0EWpaFA2eEZCGExCFgO3EcH7tLgUorWvFxjqb2pJslsYvtIPgL_GpcWgUJ4YtHM49fz8xLyGPOXnEu9WuWDy-1qu-Qc17XvFAN13fnmLH75EGMt4xJKWtxRs60LKUs63Pya0E_9W43IH3jI1Lf0YVL6CDBQC8xwYjpBqJ3SJfuBpzBSFfR70NGhj5nwVm6CT4m2A057h1dimLl7GTQ0o84wA9IvXcH4U3AhGGk6--Hh5tpGL2D8JMuQk73GB-Sex0MER-d7gvy5e3y89X74nr9bnW1uC6M0E0qZMdsZcFsWWmMtNBUNdOd3grRSaalbCTWKJgQVjS6MQJsCWitUraRwIQtL8iLo-4--G8TxtSOfTQ45P7RT7FVWqmqKWUGn_0D3vop5MFjK4RQsqr1AXp5hEzeQgzYtfvQj3mulrP2YE47m5PZpyfBaTuincmTG7n-_FSHaGDoQl54H2dM8KpUjfrb11cIOwxz_XL94c8_7d52GXryX-jYyW8bIauQ</recordid><startdate>199803</startdate><enddate>199803</enddate><creator>Gao, Yuansheng</creator><creator>Tolsa, Jean-Francois</creator><creator>Shen, Hai</creator><creator>Raj, J.Usha</creator><general>Karger</general><general>S. 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Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Animals</topic><topic>Betamethasone - administration & dosage</topic><topic>Betamethasone - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Bronchodilator Agents - pharmacology</topic><topic>Dinoprostone - pharmacology</topic><topic>Emergency and intensive care: neonates and children. Prematurity. Sudden death</topic><topic>Female</topic><topic>Intensive care medicine</topic><topic>Isoproterenol - pharmacology</topic><topic>Medical sciences</topic><topic>Original Paper</topic><topic>Pregnancy</topic><topic>Pulmonary Artery - embryology</topic><topic>Pulmonary Artery - physiology</topic><topic>Sheep</topic><topic>Vasodilation - drug effects</topic><topic>Vasodilator Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Yuansheng</creatorcontrib><creatorcontrib>Tolsa, Jean-Francois</creatorcontrib><creatorcontrib>Shen, Hai</creatorcontrib><creatorcontrib>Raj, J.Usha</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of the neonate</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Yuansheng</au><au>Tolsa, Jean-Francois</au><au>Shen, Hai</au><au>Raj, J.Usha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Single Dose of Antenatal Betamethasone Enhances Isoprenaline and Prostaglandin E2-Induced Relaxation of Preterm Ovine Pulmonary Arteries</atitle><jtitle>Biology of the neonate</jtitle><addtitle>Neonatology</addtitle><date>1998-03</date><risdate>1998</risdate><volume>73</volume><issue>3</issue><spage>182</spage><epage>189</epage><pages>182-189</pages><issn>1661-7800</issn><issn>0006-3126</issn><eissn>1661-7819</eissn><eissn>1421-9727</eissn><coden>BNEOBV</coden><abstract>β-Adrenergic agonists and prostaglandin E 2 (PGE 2 ) play an important role in perinatal pulmonary circulation. We have determined the effect of antenatal glucocorticoid treatment on isoprenaline- and PGE 2 -mediated relaxation of pulmonary arteries of newborn preterm lambs. Ovine fetuses (121 days of gestation; term = 150 days) received a single intramuscular dose of betamethasone (0.5 mg/kg) or saline. Fifteen hours after the injection, the lambs were delivered, ventilated for 3 h, and sacrificed. The fourth-generation pulmonary arteries were dissected and cut into rings for study. In endothelin-1-preconstricted vessels, isoprenaline, PGE 2 , and forskolin (an activator of adenylyl cyclase) induced greater relaxations of pulmonary arteries of betamethasone-treated lambs than those of controls. 8-Bromo-cyclic adenosine monophosphate, a cell membrane permeable analogue of cyclic adenosine monophosphate, caused similar relaxation of all vessels. When stimulated with isoprenaline and PGE 2 , the adenylyl cyclase activity of crude membrane preparations of pulmonary arteries treated with betamethasone was greater than that of controls. These results show that single-dose antenatal betamethasone treatment enhances relaxation of pulmonary arteries of preterm lambs induced by isoprenaline and PGE 2 and that an enhanced adenylyl cyclase activity contributes to the effect of betamethasone on pulmonary arteries of preterm lambs.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>9535536</pmid><doi>10.1159/000013976</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 1661-7800 |
| ispartof | Biology of the neonate, 1998-03, Vol.73 (3), p.182-189 |
| issn | 1661-7800 0006-3126 1661-7819 1421-9727 |
| language | eng |
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| source | MEDLINE; Karger Journals Complete |
| subjects | Adenylyl Cyclases - metabolism Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Betamethasone - administration & dosage Betamethasone - pharmacology Biological and medical sciences Bronchodilator Agents - pharmacology Dinoprostone - pharmacology Emergency and intensive care: neonates and children. Prematurity. Sudden death Female Intensive care medicine Isoproterenol - pharmacology Medical sciences Original Paper Pregnancy Pulmonary Artery - embryology Pulmonary Artery - physiology Sheep Vasodilation - drug effects Vasodilator Agents - pharmacology |
| title | A Single Dose of Antenatal Betamethasone Enhances Isoprenaline and Prostaglandin E2-Induced Relaxation of Preterm Ovine Pulmonary Arteries |
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