A simple and specific assay of glycosyltransferase and glycosidase activities by an enzyme-linked immunosorbent assay method, and its application to assay of galactosyltransferase activity in sera from patients with cancer

A simple, sensitive, and specific assay method for glycosyltransferase and glycosidase activities has been established by means of an enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody, H-11 directed to lactoneotetraosylceramide (nLc4Cer). Enzyme activity was determined by assaying...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 1990-03, Vol.107 (3), p.493-498
Hauptverfasser:	TAKI, T, NISHIWAKI, S, ISHII, K, HANDA, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Antibodies, Monoclonal Biological and medical sciences cancer patients Chromatography, Thin Layer Detergents Enzyme-Linked Immunosorbent Assay Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Galactosyltransferases - blood Galactosyltransferases - metabolism General aspects, investigation methods Glucosyltransferases - analysis Glucosyltransferases - blood Glucosyltransferases - metabolism glycosidase Glycoside Hydrolases - analysis Glycoside Hydrolases - blood Glycoside Hydrolases - metabolism Humans Hydrogen-Ion Concentration Immunochemistry Kinetics Neoplasms - blood Neoplasms - enzymology Neuraminidase - analysis Staining and Labeling Substrate Specificity
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