In-vitro evaluation of the antifungal activity of amphotericin B entrapped into liposomes during storage for one year
The stability of the antifungal activity of amphotericin B entrapped in small sonicated liposomes (ampholiposomes) was studied in vitro over a one-year period. Preparations of ampholiposomes stored at −20°C or at 4°C were compared monthly with freshly-prepared ampholiposomes and a commercial prepara...
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 1990-03, Vol.25 (3), p.361-366 |
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| container_start_page | 361 |
| container_title | Journal of antimicrobial chemotherapy |
| container_volume | 25 |
| creator | Heymans, C. Van der Auwera, P. Sculier, J. P. Coune, A. Brassinne, C. Laduron, C. Hollaert, C. Meunier, F. |
| description | The stability of the antifungal activity of amphotericin B entrapped in small sonicated liposomes (ampholiposomes) was studied in vitro over a one-year period. Preparations of ampholiposomes stored at −20°C or at 4°C were compared monthly with freshly-prepared ampholiposomes and a commercial preparation of amphotericin B-deoxycholate (Fungizone; Squibb) by a killing curve method with Candida albicans. The bioactivity of the four preparations, each containing 1·5 or 2 mg/l of amphotericin B, was measured as the initial rate of killing and the ‘relative bioactivity’. Relative bioactivity was calculated as the percentage reduction of the area under the growth curve compared with control growth. Storage of ampholiposomes for one year did not decrease their antifungal activity. Storage of ampholiposomes containing 1·5 mg/l amphotericin B for one year at −20°C, but not at 4°C, gave a significant increase in relative bioactivity and killing rate in comparison with freshly-prepared ampholiposomes. This was probably due to modifications in the spatial configuration of phospholipids and amphotericin B. The persisting antifungal activity of ampholiposomes stored for one year should allow the preparation of large batches to perform comparative clinical studies. |
| doi_str_mv | 10.1093/jac/25.3.361 |
| format | Article |
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P. ; Coune, A. ; Brassinne, C. ; Laduron, C. ; Hollaert, C. ; Meunier, F.</creator><creatorcontrib>Heymans, C. ; Van der Auwera, P. ; Sculier, J. P. ; Coune, A. ; Brassinne, C. ; Laduron, C. ; Hollaert, C. ; Meunier, F.</creatorcontrib><description>The stability of the antifungal activity of amphotericin B entrapped in small sonicated liposomes (ampholiposomes) was studied in vitro over a one-year period. Preparations of ampholiposomes stored at −20°C or at 4°C were compared monthly with freshly-prepared ampholiposomes and a commercial preparation of amphotericin B-deoxycholate (Fungizone; Squibb) by a killing curve method with Candida albicans. The bioactivity of the four preparations, each containing 1·5 or 2 mg/l of amphotericin B, was measured as the initial rate of killing and the ‘relative bioactivity’. Relative bioactivity was calculated as the percentage reduction of the area under the growth curve compared with control growth. Storage of ampholiposomes for one year did not decrease their antifungal activity. Storage of ampholiposomes containing 1·5 mg/l amphotericin B for one year at −20°C, but not at 4°C, gave a significant increase in relative bioactivity and killing rate in comparison with freshly-prepared ampholiposomes. This was probably due to modifications in the spatial configuration of phospholipids and amphotericin B. The persisting antifungal activity of ampholiposomes stored for one year should allow the preparation of large batches to perform comparative clinical studies.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/25.3.361</identifier><identifier>PMID: 2338414</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Amphotericin B - administration & dosage ; Amphotericin B - pharmacology ; Biological and medical sciences ; Candida albicans ; Drug Carriers ; Drug Stability ; Drug Storage ; General pharmacology ; Liposomes ; Medical sciences ; Microbial Sensitivity Tests ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Time Factors</subject><ispartof>Journal of antimicrobial chemotherapy, 1990-03, Vol.25 (3), p.361-366</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c299t-d39c67dcf94923c4b074a3e1a5c0995fd19eaaf80568f981cbc3aef96dcc4bc83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6897840$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2338414$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heymans, C.</creatorcontrib><creatorcontrib>Van der Auwera, P.</creatorcontrib><creatorcontrib>Sculier, J. P.</creatorcontrib><creatorcontrib>Coune, A.</creatorcontrib><creatorcontrib>Brassinne, C.</creatorcontrib><creatorcontrib>Laduron, C.</creatorcontrib><creatorcontrib>Hollaert, C.</creatorcontrib><creatorcontrib>Meunier, F.</creatorcontrib><title>In-vitro evaluation of the antifungal activity of amphotericin B entrapped into liposomes during storage for one year</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>The stability of the antifungal activity of amphotericin B entrapped in small sonicated liposomes (ampholiposomes) was studied in vitro over a one-year period. Preparations of ampholiposomes stored at −20°C or at 4°C were compared monthly with freshly-prepared ampholiposomes and a commercial preparation of amphotericin B-deoxycholate (Fungizone; Squibb) by a killing curve method with Candida albicans. The bioactivity of the four preparations, each containing 1·5 or 2 mg/l of amphotericin B, was measured as the initial rate of killing and the ‘relative bioactivity’. Relative bioactivity was calculated as the percentage reduction of the area under the growth curve compared with control growth. Storage of ampholiposomes for one year did not decrease their antifungal activity. Storage of ampholiposomes containing 1·5 mg/l amphotericin B for one year at −20°C, but not at 4°C, gave a significant increase in relative bioactivity and killing rate in comparison with freshly-prepared ampholiposomes. This was probably due to modifications in the spatial configuration of phospholipids and amphotericin B. The persisting antifungal activity of ampholiposomes stored for one year should allow the preparation of large batches to perform comparative clinical studies.</description><subject>Amphotericin B - administration & dosage</subject><subject>Amphotericin B - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Candida albicans</subject><subject>Drug Carriers</subject><subject>Drug Stability</subject><subject>Drug Storage</subject><subject>General pharmacology</subject><subject>Liposomes</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Time Factors</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0cFrFDEUBvAgSt1Wb16FHMRTZ5tMJpPJUYvaQkGQitJLeJt52abOJNMkU9z_3im77NXTO3w_Hrz3EfKOszVnWlw8gL2o5VqsRctfkBVvWlbVTPOXZMUEk5VqpHhNTnN-YIy1su1OyEktRNfwZkXm61A9-ZIixScYZig-BhodLfdIIRTv5rCFgYItfmG75wjG6T4WTN76QD9TDCXBNGFPfSiRDn6KOY6YaT8nH7Y0l5hgi9TFRGNAukNIb8grB0PGt4d5Rn5-_XJ7eVXdfP92ffnpprK11qXqhbat6q3Tja6FbTZMNSCQg7RMa-l6rhHAdWw5yumO240VgE63vV2w7cQZ-bjfO6X4OGMuZvTZ4jBAwDhno7RStezq_0IuZce5lgs830ObYs4JnZmSHyHtDGfmuQ6z1GFqaYRZ6lj4-8PeeTNif8SH_y_5h0MO2cLgEgTr85G1nVZdwxZW7ZnPBf8eY0h_TKuEkubq952Rd-zXj1vVGi3-AWafpFg</recordid><startdate>199003</startdate><enddate>199003</enddate><creator>Heymans, C.</creator><creator>Van der Auwera, P.</creator><creator>Sculier, J. P.</creator><creator>Coune, A.</creator><creator>Brassinne, C.</creator><creator>Laduron, C.</creator><creator>Hollaert, C.</creator><creator>Meunier, F.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>199003</creationdate><title>In-vitro evaluation of the antifungal activity of amphotericin B entrapped into liposomes during storage for one year</title><author>Heymans, C. ; Van der Auwera, P. ; Sculier, J. P. ; Coune, A. ; Brassinne, C. ; Laduron, C. ; Hollaert, C. ; Meunier, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c299t-d39c67dcf94923c4b074a3e1a5c0995fd19eaaf80568f981cbc3aef96dcc4bc83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amphotericin B - administration & dosage</topic><topic>Amphotericin B - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Candida albicans</topic><topic>Drug Carriers</topic><topic>Drug Stability</topic><topic>Drug Storage</topic><topic>General pharmacology</topic><topic>Liposomes</topic><topic>Medical sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. 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P.</au><au>Coune, A.</au><au>Brassinne, C.</au><au>Laduron, C.</au><au>Hollaert, C.</au><au>Meunier, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In-vitro evaluation of the antifungal activity of amphotericin B entrapped into liposomes during storage for one year</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>1990-03</date><risdate>1990</risdate><volume>25</volume><issue>3</issue><spage>361</spage><epage>366</epage><pages>361-366</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>The stability of the antifungal activity of amphotericin B entrapped in small sonicated liposomes (ampholiposomes) was studied in vitro over a one-year period. Preparations of ampholiposomes stored at −20°C or at 4°C were compared monthly with freshly-prepared ampholiposomes and a commercial preparation of amphotericin B-deoxycholate (Fungizone; Squibb) by a killing curve method with Candida albicans. The bioactivity of the four preparations, each containing 1·5 or 2 mg/l of amphotericin B, was measured as the initial rate of killing and the ‘relative bioactivity’. Relative bioactivity was calculated as the percentage reduction of the area under the growth curve compared with control growth. Storage of ampholiposomes for one year did not decrease their antifungal activity. Storage of ampholiposomes containing 1·5 mg/l amphotericin B for one year at −20°C, but not at 4°C, gave a significant increase in relative bioactivity and killing rate in comparison with freshly-prepared ampholiposomes. This was probably due to modifications in the spatial configuration of phospholipids and amphotericin B. The persisting antifungal activity of ampholiposomes stored for one year should allow the preparation of large batches to perform comparative clinical studies.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>2338414</pmid><doi>10.1093/jac/25.3.361</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of antimicrobial chemotherapy, 1990-03, Vol.25 (3), p.361-366 |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy |
| subjects | Amphotericin B - administration & dosage Amphotericin B - pharmacology Biological and medical sciences Candida albicans Drug Carriers Drug Stability Drug Storage General pharmacology Liposomes Medical sciences Microbial Sensitivity Tests Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Time Factors |
| title | In-vitro evaluation of the antifungal activity of amphotericin B entrapped into liposomes during storage for one year |
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