Regulation of Human B19 Parvovirus Promoter Expression by hGABP (E4TF1) Transcription Factor
The genetic expression of human B19 parvovirus is only dependent on one promoter in vivo and in vitro . This is the P6 promoter, which is located on the left side of the genome and is a single-stranded DNA molecule. This led us to investigate the regulation of the P6 promoter and the possible result...
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| Veröffentlicht in: | The Journal of biological chemistry 1998-04, Vol.273 (14), p.8287-8293 |
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| creator | Vassias, I Hazan, U Michel, Y Sawa, C Handa, H Gouya, L Morinet, F |
| description | The genetic expression of human B19 parvovirus is only dependent on one promoter in vivo and in vitro . This is the P6 promoter, which is located on the left side of the genome and is a single-stranded DNA molecule. This led
us to investigate the regulation of the P6 promoter and the possible resulting variability of the nucleotide sequence. After
analysis of the promoter region of 17 B19 strains, only 1.5% variability was found. More exciting was the finding of mutations
that were clustered around the TATA box and defined a highly conserved region (nucleotides 113â210) in the proximal part of
the P6 promoter. HeLa and UT7/Epo cell extracts were found to protect this region, which contained a core motif for Ets family
proteins, with YY1 and Sp1 binding sites on either side. Gel mobility shift assays performed with nuclear proteins from HeLa
and UT7/Epo cells identified DNA-binding proteins specific for these sites. By supershift analysis, we demonstrated the binding
of the hGABP (also named E4TF1) protein to the Ets binding site and the fixation of Sp1 and YY1 proteins on their respective
motifs. In Drosophila SL2 cells, hGABPα and -β stimulated P6 promoter activity, and hGABPα/hGABPβ and Sp1 exerted synergistic stimulation of this
activity, an effect diminished by YY1. |
| doi_str_mv | 10.1074/jbc.273.14.8287 |
| format | Article |
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us to investigate the regulation of the P6 promoter and the possible resulting variability of the nucleotide sequence. After
analysis of the promoter region of 17 B19 strains, only 1.5% variability was found. More exciting was the finding of mutations
that were clustered around the TATA box and defined a highly conserved region (nucleotides 113â210) in the proximal part of
the P6 promoter. HeLa and UT7/Epo cell extracts were found to protect this region, which contained a core motif for Ets family
proteins, with YY1 and Sp1 binding sites on either side. Gel mobility shift assays performed with nuclear proteins from HeLa
and UT7/Epo cells identified DNA-binding proteins specific for these sites. By supershift analysis, we demonstrated the binding
of the hGABP (also named E4TF1) protein to the Ets binding site and the fixation of Sp1 and YY1 proteins on their respective
motifs. In Drosophila SL2 cells, hGABPα and -β stimulated P6 promoter activity, and hGABPα/hGABPβ and Sp1 exerted synergistic stimulation of this
activity, an effect diminished by YY1.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.273.14.8287</identifier><identifier>PMID: 9525935</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Base Sequence ; DNA Primers ; GA-Binding Protein Transcription Factor ; Gene Expression Regulation, Viral ; HeLa Cells ; Humans ; Molecular Sequence Data ; Parvovirus B19, Human - physiology ; Promoter Regions, Genetic - genetics ; Transcription Factors - biosynthesis ; Transcription Factors - genetics ; Virus Replication - genetics</subject><ispartof>The Journal of biological chemistry, 1998-04, Vol.273 (14), p.8287-8293</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-3d96d8752e4236946b074362869c998e6a79087b8b4cbd29b134d1257af58cc73</citedby><cites>FETCH-LOGICAL-c502t-3d96d8752e4236946b074362869c998e6a79087b8b4cbd29b134d1257af58cc73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9525935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vassias, I</creatorcontrib><creatorcontrib>Hazan, U</creatorcontrib><creatorcontrib>Michel, Y</creatorcontrib><creatorcontrib>Sawa, C</creatorcontrib><creatorcontrib>Handa, H</creatorcontrib><creatorcontrib>Gouya, L</creatorcontrib><creatorcontrib>Morinet, F</creatorcontrib><title>Regulation of Human B19 Parvovirus Promoter Expression by hGABP (E4TF1) Transcription Factor</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The genetic expression of human B19 parvovirus is only dependent on one promoter in vivo and in vitro . This is the P6 promoter, which is located on the left side of the genome and is a single-stranded DNA molecule. This led
us to investigate the regulation of the P6 promoter and the possible resulting variability of the nucleotide sequence. After
analysis of the promoter region of 17 B19 strains, only 1.5% variability was found. More exciting was the finding of mutations
that were clustered around the TATA box and defined a highly conserved region (nucleotides 113â210) in the proximal part of
the P6 promoter. HeLa and UT7/Epo cell extracts were found to protect this region, which contained a core motif for Ets family
proteins, with YY1 and Sp1 binding sites on either side. Gel mobility shift assays performed with nuclear proteins from HeLa
and UT7/Epo cells identified DNA-binding proteins specific for these sites. By supershift analysis, we demonstrated the binding
of the hGABP (also named E4TF1) protein to the Ets binding site and the fixation of Sp1 and YY1 proteins on their respective
motifs. In Drosophila SL2 cells, hGABPα and -β stimulated P6 promoter activity, and hGABPα/hGABPβ and Sp1 exerted synergistic stimulation of this
activity, an effect diminished by YY1.</description><subject>Base Sequence</subject><subject>DNA Primers</subject><subject>GA-Binding Protein Transcription Factor</subject><subject>Gene Expression Regulation, Viral</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Parvovirus B19, Human - physiology</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Transcription Factors - biosynthesis</subject><subject>Transcription Factors - genetics</subject><subject>Virus Replication - genetics</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtLAzEQh4MoWh9nT0JAED1szXOTHFtpVShYpIIHISTZtF3pNjXZ9fHfu7VF8ORc5jDf_Ib5ADjFqIuRYNev1nWJoF3MupJIsQM6GEmaUY6fd0EHIYIzRbg8AIcpvaK2mML7YF9xwhXlHfDy6GfNwtRlWMIwhXdNZZawjxUcm_ge3svYJDiOoQq1j3DwuYo-pTVrv-D8ttcfw8sBmwzxFZxEs0wulqufqKFxdYjHYG9qFsmfbPsReBoOJjd32ejh9v6mN8ocR6TOaKHyQgpOPCM0Vyy37Wc0JzJXTinpcyMUksJKy5wtiLKYsgITLsyUS-cEPQIXm9xVDG-NT7WuyuT8YmGWPjRJCyUEVoT8C-L2qEK5asHrDehiSCn6qV7FsjLxS2Ok1-J1K1634jVmei2-3TjbRje28sUvvzXdzs8383k5m3-U0WtbBjf31Z-Ubwfqh_I</recordid><startdate>19980403</startdate><enddate>19980403</enddate><creator>Vassias, I</creator><creator>Hazan, U</creator><creator>Michel, Y</creator><creator>Sawa, C</creator><creator>Handa, H</creator><creator>Gouya, L</creator><creator>Morinet, F</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19980403</creationdate><title>Regulation of Human B19 Parvovirus Promoter Expression by hGABP (E4TF1) Transcription Factor</title><author>Vassias, I ; Hazan, U ; Michel, Y ; Sawa, C ; Handa, H ; Gouya, L ; Morinet, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-3d96d8752e4236946b074362869c998e6a79087b8b4cbd29b134d1257af58cc73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Base Sequence</topic><topic>DNA Primers</topic><topic>GA-Binding Protein Transcription Factor</topic><topic>Gene Expression Regulation, Viral</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Parvovirus B19, Human - physiology</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Transcription Factors - biosynthesis</topic><topic>Transcription Factors - genetics</topic><topic>Virus Replication - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vassias, I</creatorcontrib><creatorcontrib>Hazan, U</creatorcontrib><creatorcontrib>Michel, Y</creatorcontrib><creatorcontrib>Sawa, C</creatorcontrib><creatorcontrib>Handa, H</creatorcontrib><creatorcontrib>Gouya, L</creatorcontrib><creatorcontrib>Morinet, F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vassias, I</au><au>Hazan, U</au><au>Michel, Y</au><au>Sawa, C</au><au>Handa, H</au><au>Gouya, L</au><au>Morinet, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of Human B19 Parvovirus Promoter Expression by hGABP (E4TF1) Transcription Factor</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1998-04-03</date><risdate>1998</risdate><volume>273</volume><issue>14</issue><spage>8287</spage><epage>8293</epage><pages>8287-8293</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>The genetic expression of human B19 parvovirus is only dependent on one promoter in vivo and in vitro . This is the P6 promoter, which is located on the left side of the genome and is a single-stranded DNA molecule. This led
us to investigate the regulation of the P6 promoter and the possible resulting variability of the nucleotide sequence. After
analysis of the promoter region of 17 B19 strains, only 1.5% variability was found. More exciting was the finding of mutations
that were clustered around the TATA box and defined a highly conserved region (nucleotides 113â210) in the proximal part of
the P6 promoter. HeLa and UT7/Epo cell extracts were found to protect this region, which contained a core motif for Ets family
proteins, with YY1 and Sp1 binding sites on either side. Gel mobility shift assays performed with nuclear proteins from HeLa
and UT7/Epo cells identified DNA-binding proteins specific for these sites. By supershift analysis, we demonstrated the binding
of the hGABP (also named E4TF1) protein to the Ets binding site and the fixation of Sp1 and YY1 proteins on their respective
motifs. In Drosophila SL2 cells, hGABPα and -β stimulated P6 promoter activity, and hGABPα/hGABPβ and Sp1 exerted synergistic stimulation of this
activity, an effect diminished by YY1.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>9525935</pmid><doi>10.1074/jbc.273.14.8287</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1998-04, Vol.273 (14), p.8287-8293 |
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| source | MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Base Sequence DNA Primers GA-Binding Protein Transcription Factor Gene Expression Regulation, Viral HeLa Cells Humans Molecular Sequence Data Parvovirus B19, Human - physiology Promoter Regions, Genetic - genetics Transcription Factors - biosynthesis Transcription Factors - genetics Virus Replication - genetics |
| title | Regulation of Human B19 Parvovirus Promoter Expression by hGABP (E4TF1) Transcription Factor |
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