Ethanol increases the firing rate of dopamine neurons of the rat ventral tegmental area in vitro
The ventral tegmental area (VTA) is a brain region rich in dopamine-containing neurons. Since most agents which act as substrates for self-administration increase dopaminergic outflow in the mesolimbic or mesocortical areas, the VTA slice preparation may be useful for identifying drugs with potentia...
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| Veröffentlicht in: | Brain research 1990-01, Vol.508 (1), p.65-69 |
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| container_title | Brain research |
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| creator | Brodie, Mark S. Shefner, Sarah A. Dunwiddie, Thomas V. |
| description | The ventral tegmental area (VTA) is a brain region rich in dopamine-containing neurons. Since most agents which act as substrates for self-administration increase dopaminergic outflow in the mesolimbic or mesocortical areas, the VTA slice preparation may be useful for identifying drugs with potential for abuse. While ethanol (EtOH) is a drug of abuse which has been widely studied, the properties of ethanol which contribute to its abuse potential are not known. We have developed a brain slice preparation of the VTA in order to study the action of EtOH on putative dopamine neurons. Concentrations of EtOH from 20 to 320 mM produce a dose-dependent excitation of the dopamine-type neurons of the VTA. About 89% of neurons which have electrophysiological characteristics established for presumed dopamine-containing neurons were excited by ethanol in the pharmacologically relevant concentration range. This excitation persists in low-calcium, high-magnesium medium, which suggests a direct excitatory action of EtOH on dopamine-type cells in the VTA slice. |
| doi_str_mv | 10.1016/0006-8993(90)91118-Z |
| format | Article |
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Since most agents which act as substrates for self-administration increase dopaminergic outflow in the mesolimbic or mesocortical areas, the VTA slice preparation may be useful for identifying drugs with potential for abuse. While ethanol (EtOH) is a drug of abuse which has been widely studied, the properties of ethanol which contribute to its abuse potential are not known. We have developed a brain slice preparation of the VTA in order to study the action of EtOH on putative dopamine neurons. Concentrations of EtOH from 20 to 320 mM produce a dose-dependent excitation of the dopamine-type neurons of the VTA. About 89% of neurons which have electrophysiological characteristics established for presumed dopamine-containing neurons were excited by ethanol in the pharmacologically relevant concentration range. 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(Biomedical Division)</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-c84ec81531227cfa6d91e54878e8c281c1a505cac060d804125652db9dd455e63</citedby><cites>FETCH-LOGICAL-c449t-c84ec81531227cfa6d91e54878e8c281c1a505cac060d804125652db9dd455e63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/000689939091118Z$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6818142$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2337793$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brodie, Mark S.</creatorcontrib><creatorcontrib>Shefner, Sarah A.</creatorcontrib><creatorcontrib>Dunwiddie, Thomas V.</creatorcontrib><title>Ethanol increases the firing rate of dopamine neurons of the rat ventral tegmental area in vitro</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>The ventral tegmental area (VTA) is a brain region rich in dopamine-containing neurons. Since most agents which act as substrates for self-administration increase dopaminergic outflow in the mesolimbic or mesocortical areas, the VTA slice preparation may be useful for identifying drugs with potential for abuse. While ethanol (EtOH) is a drug of abuse which has been widely studied, the properties of ethanol which contribute to its abuse potential are not known. We have developed a brain slice preparation of the VTA in order to study the action of EtOH on putative dopamine neurons. Concentrations of EtOH from 20 to 320 mM produce a dose-dependent excitation of the dopamine-type neurons of the VTA. About 89% of neurons which have electrophysiological characteristics established for presumed dopamine-containing neurons were excited by ethanol in the pharmacologically relevant concentration range. This excitation persists in low-calcium, high-magnesium medium, which suggests a direct excitatory action of EtOH on dopamine-type cells in the VTA slice.</description><subject>Action Potentials - drug effects</subject><subject>Alcoholism and acute alcohol poisoning</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain slice</subject><subject>Dopamine</subject><subject>Dopamine - physiology</subject><subject>Electrophysiology</subject><subject>Ethanol</subject><subject>Ethanol - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Medical sciences</subject><subject>Mesencephalon</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Reward</subject><subject>Tegmentum Mesencephali - drug effects</subject><subject>Tegmentum Mesencephali - physiology</subject><subject>Toxicology</subject><subject>Ventral tegmental area</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo67j6DxRyENFDa74_LoIsu6uw4EUve4nZpHo30p0ek8yA_960M8xRT1WV96mX8BZCLyl5TwlVHwghajDW8reWvLOUUjPcPkIbajQbFBPkMdqckKfoWa0_-8i5JWfojHGuteUb9OOyPfi8TDjlUMBXqLg9AB5TSfkeF98ALyOOy9bPKQPOsCtLruvbinUd7yG34ifc4H7ube98N-p-eJ9aWZ6jJ6OfKrw41nP0_ery28Xn4ebr9ZeLTzdDEMK2IRgBwVDJKWM6jF5FS0EKow2YwAwN1Esigw9EkWiIoEwqyeKdjVFICYqfozcH321Zfu2gNjenGmCafIZlV522WlMu7X9BKpUh1ugOigMYylJrgdFtS5p9-e0ocesF3BqvW-N1lri_F3C3fe3V0X93N0M8LR0j7_rro-5r8NNYfA6pnjBlqKGCdezjAYMe2j5BcTUkyAFiKhCai0v69z_-ABVAocQ</recordid><startdate>19900101</startdate><enddate>19900101</enddate><creator>Brodie, Mark S.</creator><creator>Shefner, Sarah A.</creator><creator>Dunwiddie, Thomas V.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19900101</creationdate><title>Ethanol increases the firing rate of dopamine neurons of the rat ventral tegmental area in vitro</title><author>Brodie, Mark S. ; Shefner, Sarah A. ; Dunwiddie, Thomas V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-c84ec81531227cfa6d91e54878e8c281c1a505cac060d804125652db9dd455e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Action Potentials - drug effects</topic><topic>Alcoholism and acute alcohol poisoning</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain slice</topic><topic>Dopamine</topic><topic>Dopamine - physiology</topic><topic>Electrophysiology</topic><topic>Ethanol</topic><topic>Ethanol - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Medical sciences</topic><topic>Mesencephalon</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Reward</topic><topic>Tegmentum Mesencephali - drug effects</topic><topic>Tegmentum Mesencephali - physiology</topic><topic>Toxicology</topic><topic>Ventral tegmental area</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brodie, Mark S.</creatorcontrib><creatorcontrib>Shefner, Sarah A.</creatorcontrib><creatorcontrib>Dunwiddie, Thomas V.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brodie, Mark S.</au><au>Shefner, Sarah A.</au><au>Dunwiddie, Thomas V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ethanol increases the firing rate of dopamine neurons of the rat ventral tegmental area in vitro</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1990-01-01</date><risdate>1990</risdate><volume>508</volume><issue>1</issue><spage>65</spage><epage>69</epage><pages>65-69</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>The ventral tegmental area (VTA) is a brain region rich in dopamine-containing neurons. Since most agents which act as substrates for self-administration increase dopaminergic outflow in the mesolimbic or mesocortical areas, the VTA slice preparation may be useful for identifying drugs with potential for abuse. While ethanol (EtOH) is a drug of abuse which has been widely studied, the properties of ethanol which contribute to its abuse potential are not known. We have developed a brain slice preparation of the VTA in order to study the action of EtOH on putative dopamine neurons. Concentrations of EtOH from 20 to 320 mM produce a dose-dependent excitation of the dopamine-type neurons of the VTA. About 89% of neurons which have electrophysiological characteristics established for presumed dopamine-containing neurons were excited by ethanol in the pharmacologically relevant concentration range. This excitation persists in low-calcium, high-magnesium medium, which suggests a direct excitatory action of EtOH on dopamine-type cells in the VTA slice.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2337793</pmid><doi>10.1016/0006-8993(90)91118-Z</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Action Potentials - drug effects Alcoholism and acute alcohol poisoning Animals Biological and medical sciences Brain slice Dopamine Dopamine - physiology Electrophysiology Ethanol Ethanol - pharmacology In Vitro Techniques Medical sciences Mesencephalon Rats Rats, Inbred Strains Reward Tegmentum Mesencephali - drug effects Tegmentum Mesencephali - physiology Toxicology Ventral tegmental area |
| title | Ethanol increases the firing rate of dopamine neurons of the rat ventral tegmental area in vitro |
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