[27] Analysis of chaperone function using citrate synthase as nonnative substrate protein

The chapter presents a study related to the analysis of chaperone function by using citrate synthase (CS) as nonnative substrate protein. The chapter describes the use of CS as a chaperone substrate. CS is well suited, because unfolding and refolding can be easily monitored and both the thermal unfo...

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Veröffentlicht in:	Methods in Enzymology 1998, Vol.290, p.323-338
Hauptverfasser:	Buchner, Johannes, Grallert, Holger, Jakob, Ursula
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chaperonin 10 - metabolism Chaperonin 60 - metabolism Citrate (si)-Synthase - metabolism Enzyme Activation - physiology Enzyme Stability - physiology Guanidine - pharmacology Heat-Shock Proteins - physiology HSP90 Heat-Shock Proteins - metabolism Kinetics Mitochondria, Heart - enzymology Molecular Chaperones - physiology Oxaloacetates - pharmacology Protein Conformation Protein Denaturation Protein Folding Scattering, Radiation Spectrometry, Fluorescence Swine Temperature
Online-Zugang:	Volltext
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