The last few milliseconds in the life of a secretory granule. Docking, dynamics and fusion visualized by total internal reflection fluorescence microscopy (TIRFM)

We have monitored single vesicles (granules) in bovine adrenal chromaffin cells using an optical sectioning technique, total internal reflection fluorescence microscopy (TIRFM). With TIR, fluorescence excitation is limited to an optical slice near a glass/water interface. In cells located at the int...

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Veröffentlicht in:	European biophysics journal 1998-01, Vol.27 (2), p.83-98
Hauptverfasser:	Oheim, M, Loerke, D, Stühmer, W, Chow, R H
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bioassays Biophysics Calibration Cattle Cellular biology Chromaffin Cells - ultrastructure Cytoplasmic Granules - physiology Fluorescence Fluorescence microscopy Membranes Microscopy, Fluorescence - methods Optics and Photonics Proteins Time Factors
Online-Zugang:	Volltext
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