Delayed rectifier potassium currents induced in activated rat microglia set the resting membrane potential
The delayed rectifying outward K + ( I K) current was measured in lipopolysaccharide (LPS)-activated cultured rat microglial cells by using whole-cell patch clamp method. The current showed `window current' where channels were available for activation but never fully inactivated. At near restin...
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| Veröffentlicht in: | Neuroscience letters 1998-02, Vol.242 (2), p.73-76 |
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| container_title | Neuroscience letters |
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| creator | Chung, Sungkwon Joe, Eunhye Soh, Heun Lee, Moo-Yeol Hyo-Weon Bang |
| description | The delayed rectifying outward K
+ (
I
K) current was measured in lipopolysaccharide (LPS)-activated cultured rat microglial cells by using whole-cell patch clamp method. The current showed `window current' where channels were available for activation but never fully inactivated. At near resting membrane potential some part of the current was able to be activated by depolarization. Among the several K
+ channel blockers tested, only 4-aminopyridine (4-AP) was able to block most of the current and depolarize the membrane potential reversibly. These results suggest that 4-AP sensitive
I
K current plays a direct role of setting the resting membrane potential in LPS-activated microglia. |
| doi_str_mv | 10.1016/S0304-3940(98)00029-9 |
| format | Article |
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+ (
I
K) current was measured in lipopolysaccharide (LPS)-activated cultured rat microglial cells by using whole-cell patch clamp method. The current showed `window current' where channels were available for activation but never fully inactivated. At near resting membrane potential some part of the current was able to be activated by depolarization. Among the several K
+ channel blockers tested, only 4-aminopyridine (4-AP) was able to block most of the current and depolarize the membrane potential reversibly. These results suggest that 4-AP sensitive
I
K current plays a direct role of setting the resting membrane potential in LPS-activated microglia.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/S0304-3940(98)00029-9</identifier><identifier>PMID: 9533397</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>4-Aminopyridine ; 4-Aminopyridine - pharmacology ; Animals ; Astrocytes - cytology ; Biological and medical sciences ; Cells, Cultured ; Delayed rectifier K + channel ; Delayed Rectifier Potassium Channels ; Fundamental and applied biological sciences. Psychology ; Ion Channel Gating - physiology ; Isolated neuron and nerve. Neuroglia ; Lipopolysaccharide ; Lipopolysaccharides ; Membrane Potentials - physiology ; Microglia ; Microglia - chemistry ; Microglia - drug effects ; Microglia - physiology ; Patch-Clamp Techniques ; Potassium Channels - physiology ; Potassium Channels, Voltage-Gated ; Rats ; Rats, Sprague-Dawley ; Resting membrane potential ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 1998-02, Vol.242 (2), p.73-76</ispartof><rights>1998 Elsevier Science Ireland Ltd</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c563t-5e149d1c55e243c74492fe06f3c679cd9c7bf9610283421b52cd9cc74ac54b23</citedby><cites>FETCH-LOGICAL-c563t-5e149d1c55e243c74492fe06f3c679cd9c7bf9610283421b52cd9cc74ac54b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0304-3940(98)00029-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2158825$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9533397$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chung, Sungkwon</creatorcontrib><creatorcontrib>Joe, Eunhye</creatorcontrib><creatorcontrib>Soh, Heun</creatorcontrib><creatorcontrib>Lee, Moo-Yeol</creatorcontrib><creatorcontrib>Hyo-Weon Bang</creatorcontrib><title>Delayed rectifier potassium currents induced in activated rat microglia set the resting membrane potential</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>The delayed rectifying outward K
+ (
I
K) current was measured in lipopolysaccharide (LPS)-activated cultured rat microglial cells by using whole-cell patch clamp method. The current showed `window current' where channels were available for activation but never fully inactivated. At near resting membrane potential some part of the current was able to be activated by depolarization. Among the several K
+ channel blockers tested, only 4-aminopyridine (4-AP) was able to block most of the current and depolarize the membrane potential reversibly. These results suggest that 4-AP sensitive
I
K current plays a direct role of setting the resting membrane potential in LPS-activated microglia.</description><subject>4-Aminopyridine</subject><subject>4-Aminopyridine - pharmacology</subject><subject>Animals</subject><subject>Astrocytes - cytology</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Delayed rectifier K + channel</subject><subject>Delayed Rectifier Potassium Channels</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ion Channel Gating - physiology</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>Lipopolysaccharide</subject><subject>Lipopolysaccharides</subject><subject>Membrane Potentials - physiology</subject><subject>Microglia</subject><subject>Microglia - chemistry</subject><subject>Microglia - drug effects</subject><subject>Microglia - physiology</subject><subject>Patch-Clamp Techniques</subject><subject>Potassium Channels - physiology</subject><subject>Potassium Channels, Voltage-Gated</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Resting membrane potential</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtr3DAUhUVJSSdpf0JAixKahVu9ba1CSfMoBLpo9kKWr1MFPyaSHMi_73VmmG1WAt3vHh2dQ8gZZ9854-bHXyaZqqRV7JttLhhjwlb2A9nwphZVbWtxRDYH5BM5yfkJIc21OibHVkspbb0hT79g8K_Q0QShxD5Cotu5-JzjMtKwpARTyTRO3RIQihP1iL34sm74QscY0vw4RE8zFFr-AerkEqdHOsLYJj_BKoca0Q-fycfeDxm-7M9T8nBz_XB1V93_uf199fO-CtrIUmngynY8aA1CyVArZUUPzPQymNqGzoa67a3hTDRSCd5qsd4h54NWrZCn5Hwnu03z84Ju3BhzgGFAM_OSHUZjrGHmXZAbxaW2DEG9A_GvOSfo3TbF0adXx5lbu3BvXbg1aGcb99aFs7h3tn9gaUfoDlv78HH-dT_3Ofihx7hCzAdMcN00QiN2ucMAQ3vBhlwOESbsI66luW6O7xj5D501pvk</recordid><startdate>19980213</startdate><enddate>19980213</enddate><creator>Chung, Sungkwon</creator><creator>Joe, Eunhye</creator><creator>Soh, Heun</creator><creator>Lee, Moo-Yeol</creator><creator>Hyo-Weon Bang</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19980213</creationdate><title>Delayed rectifier potassium currents induced in activated rat microglia set the resting membrane potential</title><author>Chung, Sungkwon ; Joe, Eunhye ; Soh, Heun ; Lee, Moo-Yeol ; Hyo-Weon Bang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c563t-5e149d1c55e243c74492fe06f3c679cd9c7bf9610283421b52cd9cc74ac54b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>4-Aminopyridine</topic><topic>4-Aminopyridine - pharmacology</topic><topic>Animals</topic><topic>Astrocytes - cytology</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Delayed rectifier K + channel</topic><topic>Delayed Rectifier Potassium Channels</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ion Channel Gating - physiology</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>Lipopolysaccharide</topic><topic>Lipopolysaccharides</topic><topic>Membrane Potentials - physiology</topic><topic>Microglia</topic><topic>Microglia - chemistry</topic><topic>Microglia - drug effects</topic><topic>Microglia - physiology</topic><topic>Patch-Clamp Techniques</topic><topic>Potassium Channels - physiology</topic><topic>Potassium Channels, Voltage-Gated</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Resting membrane potential</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chung, Sungkwon</creatorcontrib><creatorcontrib>Joe, Eunhye</creatorcontrib><creatorcontrib>Soh, Heun</creatorcontrib><creatorcontrib>Lee, Moo-Yeol</creatorcontrib><creatorcontrib>Hyo-Weon Bang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chung, Sungkwon</au><au>Joe, Eunhye</au><au>Soh, Heun</au><au>Lee, Moo-Yeol</au><au>Hyo-Weon Bang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Delayed rectifier potassium currents induced in activated rat microglia set the resting membrane potential</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1998-02-13</date><risdate>1998</risdate><volume>242</volume><issue>2</issue><spage>73</spage><epage>76</epage><pages>73-76</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>The delayed rectifying outward K
+ (
I
K) current was measured in lipopolysaccharide (LPS)-activated cultured rat microglial cells by using whole-cell patch clamp method. The current showed `window current' where channels were available for activation but never fully inactivated. At near resting membrane potential some part of the current was able to be activated by depolarization. Among the several K
+ channel blockers tested, only 4-aminopyridine (4-AP) was able to block most of the current and depolarize the membrane potential reversibly. These results suggest that 4-AP sensitive
I
K current plays a direct role of setting the resting membrane potential in LPS-activated microglia.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>9533397</pmid><doi>10.1016/S0304-3940(98)00029-9</doi><tpages>4</tpages></addata></record> |
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| ispartof | Neuroscience letters, 1998-02, Vol.242 (2), p.73-76 |
| issn | 0304-3940 1872-7972 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 4-Aminopyridine 4-Aminopyridine - pharmacology Animals Astrocytes - cytology Biological and medical sciences Cells, Cultured Delayed rectifier K + channel Delayed Rectifier Potassium Channels Fundamental and applied biological sciences. Psychology Ion Channel Gating - physiology Isolated neuron and nerve. Neuroglia Lipopolysaccharide Lipopolysaccharides Membrane Potentials - physiology Microglia Microglia - chemistry Microglia - drug effects Microglia - physiology Patch-Clamp Techniques Potassium Channels - physiology Potassium Channels, Voltage-Gated Rats Rats, Sprague-Dawley Resting membrane potential Vertebrates: nervous system and sense organs |
| title | Delayed rectifier potassium currents induced in activated rat microglia set the resting membrane potential |
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