Quantitative energy-filtered image analysis in cytochemistry: II. Morphometric analysis of element-distribution images
A combination of energy-filtered electron microscopy (EFEM) and an image-analysis system (IBAS/2000) is used for a morphometric analysis of chemical reaction products in cells. Electron energy-loss spectroscopic element-distribution images are acquired from cytochemical reaction products in a variet...
Gespeichert in:
| Veröffentlicht in: | Ultramicroscopy 1990, Vol.32 (1), p.69-79 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 79 |
|---|---|
| container_issue | 1 |
| container_start_page | 69 |
| container_title | Ultramicroscopy |
| container_volume | 32 |
| creator | Sorber, C.W.J. Van Dort, J.B. Ringeling, P.C. Cleton-Soeteman, M.I. de Bruijn, W.C. |
| description | A combination of energy-filtered electron microscopy (EFEM) and an image-analysis system (IBAS/2000) is used for a morphometric analysis of chemical reaction products in cells. Electron energy-loss spectroscopic element-distribution images are acquired from cytochemical reaction products in a variety of cellular objects: (1) colloidal thorium particles in extra-cellular coat material, (2) iron-containing ferritin particles in liver parenchymal cells, (3) barium-containing reaction products in endoplasmic reticulum stacks, (4) elements present in lysosomal cerium- and barium-containing precipitates connected with acid phosphatase (AcPase) or aryl sulphatase (AS) enzyme activity. Areas or area fractions are determined from such element-distribution images by application of an objective image segmentation method. By superposition of two or more element-distribution images, mutual element relations are qualitatively established in lysosomal cerium- and barium-containing precipitates connected with acid phosphatase (AcPase) or aryl sulphatase (AS) enzyme activity. By comparing electron spectroscopic images (ESI) with element-distribution images, the mutual contrast per elemeny relations are quantitatively investigated. The obtained gain in resolution in such electron energy-loss spectroscopic element-distribution images will be explained and discussed. |
| doi_str_mv | 10.1016/0304-3991(90)90095-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_79768445</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0304399190900954</els_id><sourcerecordid>79768445</sourcerecordid><originalsourceid>FETCH-LOGICAL-e205t-a8aa3d9a22dd26703a769dd10475c330ac070f5c5e344b5d930e0ad0dc5bb12a3</originalsourceid><addsrcrecordid>eNpFkV-L1DAUxYMo6-zoN1Dog8j60PWmaZpmHwRZ_DOwIoI-h9vkdjfSpmOSDvTb23GG9ek-nB-Hc-5h7BWHaw68eQ8C6lJoza80vNMAWpb1E7bhrdJlpSrxlG0ekefsMqXfAMChbi_YRSVEo4TcsMOPGUP2GbM_UEGB4v1S9n7IFMkVfsR7KjDgsCSfCh8Ku-TJPtDoU47LTbHbXRffprh_mEbK0dv_7NQXNNBIIZfuCPtuzn4KJ8v0gj3rcUj08ny37NfnTz9vv5Z337_sbj_elVSBzCW2iMJprCrnqkaBQNVo59YSSlohAC0o6KWVJOq6k04LIEAHzsqu4xWKLXt78t3H6c9MKZs1uaVhwEDTnIzSqmnrWq7g6zM4dyM5s49r0LiY86NW_c1Zx2Rx6CMG69Mj1mjetiu4ZR9OGK2lDp6iSdZTsOR8JJuNm7zhYI77meM45jiO0WD-7Wdq8Rdjn45F</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79768445</pqid></control><display><type>article</type><title>Quantitative energy-filtered image analysis in cytochemistry: II. Morphometric analysis of element-distribution images</title><source>MEDLINE</source><source>ScienceDirect Freedom Collection (Elsevier)</source><creator>Sorber, C.W.J. ; Van Dort, J.B. ; Ringeling, P.C. ; Cleton-Soeteman, M.I. ; de Bruijn, W.C.</creator><creatorcontrib>Sorber, C.W.J. ; Van Dort, J.B. ; Ringeling, P.C. ; Cleton-Soeteman, M.I. ; de Bruijn, W.C.</creatorcontrib><description>A combination of energy-filtered electron microscopy (EFEM) and an image-analysis system (IBAS/2000) is used for a morphometric analysis of chemical reaction products in cells. Electron energy-loss spectroscopic element-distribution images are acquired from cytochemical reaction products in a variety of cellular objects: (1) colloidal thorium particles in extra-cellular coat material, (2) iron-containing ferritin particles in liver parenchymal cells, (3) barium-containing reaction products in endoplasmic reticulum stacks, (4) elements present in lysosomal cerium- and barium-containing precipitates connected with acid phosphatase (AcPase) or aryl sulphatase (AS) enzyme activity. Areas or area fractions are determined from such element-distribution images by application of an objective image segmentation method. By superposition of two or more element-distribution images, mutual element relations are qualitatively established in lysosomal cerium- and barium-containing precipitates connected with acid phosphatase (AcPase) or aryl sulphatase (AS) enzyme activity. By comparing electron spectroscopic images (ESI) with element-distribution images, the mutual contrast per elemeny relations are quantitatively investigated. The obtained gain in resolution in such electron energy-loss spectroscopic element-distribution images will be explained and discussed.</description><identifier>ISSN: 0304-3991</identifier><identifier>EISSN: 1879-2723</identifier><identifier>DOI: 10.1016/0304-3991(90)90095-4</identifier><identifier>PMID: 2336735</identifier><identifier>CODEN: ULTRD6</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical biochemistry: general aspects, technics, instrumentation ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Cells, Cultured ; Epithelium - ultrastructure ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Image Processing, Computer-Assisted ; Liver - ultrastructure ; Macrophages - ultrastructure ; Mice ; Microscopy, Electron ; Rats ; Vagina - ultrastructure</subject><ispartof>Ultramicroscopy, 1990, Vol.32 (1), p.69-79</ispartof><rights>1990</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0304-3991(90)90095-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,4024,4050,4051,23930,23931,25140,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6918867$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2336735$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sorber, C.W.J.</creatorcontrib><creatorcontrib>Van Dort, J.B.</creatorcontrib><creatorcontrib>Ringeling, P.C.</creatorcontrib><creatorcontrib>Cleton-Soeteman, M.I.</creatorcontrib><creatorcontrib>de Bruijn, W.C.</creatorcontrib><title>Quantitative energy-filtered image analysis in cytochemistry: II. Morphometric analysis of element-distribution images</title><title>Ultramicroscopy</title><addtitle>Ultramicroscopy</addtitle><description>A combination of energy-filtered electron microscopy (EFEM) and an image-analysis system (IBAS/2000) is used for a morphometric analysis of chemical reaction products in cells. Electron energy-loss spectroscopic element-distribution images are acquired from cytochemical reaction products in a variety of cellular objects: (1) colloidal thorium particles in extra-cellular coat material, (2) iron-containing ferritin particles in liver parenchymal cells, (3) barium-containing reaction products in endoplasmic reticulum stacks, (4) elements present in lysosomal cerium- and barium-containing precipitates connected with acid phosphatase (AcPase) or aryl sulphatase (AS) enzyme activity. Areas or area fractions are determined from such element-distribution images by application of an objective image segmentation method. By superposition of two or more element-distribution images, mutual element relations are qualitatively established in lysosomal cerium- and barium-containing precipitates connected with acid phosphatase (AcPase) or aryl sulphatase (AS) enzyme activity. By comparing electron spectroscopic images (ESI) with element-distribution images, the mutual contrast per elemeny relations are quantitatively investigated. The obtained gain in resolution in such electron energy-loss spectroscopic element-distribution images will be explained and discussed.</description><subject>Analytical biochemistry: general aspects, technics, instrumentation</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Epithelium - ultrastructure</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Image Processing, Computer-Assisted</subject><subject>Liver - ultrastructure</subject><subject>Macrophages - ultrastructure</subject><subject>Mice</subject><subject>Microscopy, Electron</subject><subject>Rats</subject><subject>Vagina - ultrastructure</subject><issn>0304-3991</issn><issn>1879-2723</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkV-L1DAUxYMo6-zoN1Dog8j60PWmaZpmHwRZ_DOwIoI-h9vkdjfSpmOSDvTb23GG9ek-nB-Hc-5h7BWHaw68eQ8C6lJoza80vNMAWpb1E7bhrdJlpSrxlG0ekefsMqXfAMChbi_YRSVEo4TcsMOPGUP2GbM_UEGB4v1S9n7IFMkVfsR7KjDgsCSfCh8Ku-TJPtDoU47LTbHbXRffprh_mEbK0dv_7NQXNNBIIZfuCPtuzn4KJ8v0gj3rcUj08ny37NfnTz9vv5Z337_sbj_elVSBzCW2iMJprCrnqkaBQNVo59YSSlohAC0o6KWVJOq6k04LIEAHzsqu4xWKLXt78t3H6c9MKZs1uaVhwEDTnIzSqmnrWq7g6zM4dyM5s49r0LiY86NW_c1Zx2Rx6CMG69Mj1mjetiu4ZR9OGK2lDp6iSdZTsOR8JJuNm7zhYI77meM45jiO0WD-7Wdq8Rdjn45F</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Sorber, C.W.J.</creator><creator>Van Dort, J.B.</creator><creator>Ringeling, P.C.</creator><creator>Cleton-Soeteman, M.I.</creator><creator>de Bruijn, W.C.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Quantitative energy-filtered image analysis in cytochemistry: II. Morphometric analysis of element-distribution images</title><author>Sorber, C.W.J. ; Van Dort, J.B. ; Ringeling, P.C. ; Cleton-Soeteman, M.I. ; de Bruijn, W.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e205t-a8aa3d9a22dd26703a769dd10475c330ac070f5c5e344b5d930e0ad0dc5bb12a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Analytical biochemistry: general aspects, technics, instrumentation</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Epithelium - ultrastructure</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Image Processing, Computer-Assisted</topic><topic>Liver - ultrastructure</topic><topic>Macrophages - ultrastructure</topic><topic>Mice</topic><topic>Microscopy, Electron</topic><topic>Rats</topic><topic>Vagina - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sorber, C.W.J.</creatorcontrib><creatorcontrib>Van Dort, J.B.</creatorcontrib><creatorcontrib>Ringeling, P.C.</creatorcontrib><creatorcontrib>Cleton-Soeteman, M.I.</creatorcontrib><creatorcontrib>de Bruijn, W.C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Ultramicroscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sorber, C.W.J.</au><au>Van Dort, J.B.</au><au>Ringeling, P.C.</au><au>Cleton-Soeteman, M.I.</au><au>de Bruijn, W.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative energy-filtered image analysis in cytochemistry: II. Morphometric analysis of element-distribution images</atitle><jtitle>Ultramicroscopy</jtitle><addtitle>Ultramicroscopy</addtitle><date>1990</date><risdate>1990</risdate><volume>32</volume><issue>1</issue><spage>69</spage><epage>79</epage><pages>69-79</pages><issn>0304-3991</issn><eissn>1879-2723</eissn><coden>ULTRD6</coden><abstract>A combination of energy-filtered electron microscopy (EFEM) and an image-analysis system (IBAS/2000) is used for a morphometric analysis of chemical reaction products in cells. Electron energy-loss spectroscopic element-distribution images are acquired from cytochemical reaction products in a variety of cellular objects: (1) colloidal thorium particles in extra-cellular coat material, (2) iron-containing ferritin particles in liver parenchymal cells, (3) barium-containing reaction products in endoplasmic reticulum stacks, (4) elements present in lysosomal cerium- and barium-containing precipitates connected with acid phosphatase (AcPase) or aryl sulphatase (AS) enzyme activity. Areas or area fractions are determined from such element-distribution images by application of an objective image segmentation method. By superposition of two or more element-distribution images, mutual element relations are qualitatively established in lysosomal cerium- and barium-containing precipitates connected with acid phosphatase (AcPase) or aryl sulphatase (AS) enzyme activity. By comparing electron spectroscopic images (ESI) with element-distribution images, the mutual contrast per elemeny relations are quantitatively investigated. The obtained gain in resolution in such electron energy-loss spectroscopic element-distribution images will be explained and discussed.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2336735</pmid><doi>10.1016/0304-3991(90)90095-4</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3991 |
| ispartof | Ultramicroscopy, 1990, Vol.32 (1), p.69-79 |
| issn | 0304-3991 1879-2723 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79768445 |
| source | MEDLINE; ScienceDirect Freedom Collection (Elsevier) |
| subjects | Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cells, Cultured Epithelium - ultrastructure Female Fundamental and applied biological sciences. Psychology Humans Image Processing, Computer-Assisted Liver - ultrastructure Macrophages - ultrastructure Mice Microscopy, Electron Rats Vagina - ultrastructure |
| title | Quantitative energy-filtered image analysis in cytochemistry: II. Morphometric analysis of element-distribution images |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T06%3A11%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantitative%20energy-filtered%20image%20analysis%20in%20cytochemistry:%20II.%20Morphometric%20analysis%20of%20element-distribution%20images&rft.jtitle=Ultramicroscopy&rft.au=Sorber,%20C.W.J.&rft.date=1990&rft.volume=32&rft.issue=1&rft.spage=69&rft.epage=79&rft.pages=69-79&rft.issn=0304-3991&rft.eissn=1879-2723&rft.coden=ULTRD6&rft_id=info:doi/10.1016/0304-3991(90)90095-4&rft_dat=%3Cproquest_pubme%3E79768445%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=79768445&rft_id=info:pmid/2336735&rft_els_id=0304399190900954&rfr_iscdi=true |