A novel and sensitive assay for phospholipase D in intact cells
A novel and sensitive assay for phospholipase D (PLD) that measures the incorporation of high specific activity [ 3H]butan-1-ol into [ 3H]phosphatidylbutanol has been developed. The assay has been used to measure PLD activation in human neutrophils and platelets. Both the chemotactic peptide fMet-Le...
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| Veröffentlicht in: | FEBS letters 1990-05, Vol.264 (1), p.87-90 |
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| creator | Randall, Roger W. Bonser, Robert W. Thompson, Neil T. Garland, Lawrence G. |
| description | A novel and sensitive assay for phospholipase D (PLD) that measures the incorporation of high specific activity [
3H]butan-1-ol into [
3H]phosphatidylbutanol has been developed. The assay has been used to measure PLD activation in human neutrophils and platelets. Both the chemotactic peptide fMet-Leu-Phe and opsonised-zymosan stimulated PLD in the human neutrophil. In the platelet, PLD was stimulated by thrombin and collagen but responses were small and only occurred at high agonist concentrations. This assay has a number of advantages over existing techniques and should be valuable for investigating PLD activation in a variety of isolated cells and possibly intact tissues. |
| doi_str_mv | 10.1016/0014-5793(90)80772-B |
| format | Article |
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3H]butan-1-ol into [
3H]phosphatidylbutanol has been developed. The assay has been used to measure PLD activation in human neutrophils and platelets. Both the chemotactic peptide fMet-Leu-Phe and opsonised-zymosan stimulated PLD in the human neutrophil. In the platelet, PLD was stimulated by thrombin and collagen but responses were small and only occurred at high agonist concentrations. This assay has a number of advantages over existing techniques and should be valuable for investigating PLD activation in a variety of isolated cells and possibly intact tissues.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(90)80772-B</identifier><identifier>PMID: 2186929</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>1-Butanol ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Blood Platelets - enzymology ; Butanols ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydrolases ; Kinetics ; leukocytes (neutrophilic) ; N-Formylmethionine Leucyl-Phenylalanine - pharmacology ; Neutrophil ; Neutrophils - enzymology ; Opsonised-zymosan ; PAF, platelet-activating factor (1-O-hexadecyl-2-acetyl-sn-glycerophosphorylcholine) ; PBut, phosphatidylbutanol ; Phospholipase D ; Phospholipase D - analysis ; Phospholipase D - blood ; Phospholipases - blood ; Platelet ; platelets ; PLD, phospholipase D ; Radioisotope Dilution Technique ; TLC, thin-layer chromatography ; Transphosphatidylation ; Tritium ; Zymosan - pharmacology</subject><ispartof>FEBS letters, 1990-05, Vol.264 (1), p.87-90</ispartof><rights>1990</rights><rights>FEBS Letters 264 (1990) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527B-c1c6baf27d781aa1bd6629b076ee28354c75b6b0e8817e5abd4fb3bade280fc43</citedby><cites>FETCH-LOGICAL-c527B-c1c6baf27d781aa1bd6629b076ee28354c75b6b0e8817e5abd4fb3bade280fc43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(90)80772-B$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19763211$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2186929$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Randall, Roger W.</creatorcontrib><creatorcontrib>Bonser, Robert W.</creatorcontrib><creatorcontrib>Thompson, Neil T.</creatorcontrib><creatorcontrib>Garland, Lawrence G.</creatorcontrib><title>A novel and sensitive assay for phospholipase D in intact cells</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>A novel and sensitive assay for phospholipase D (PLD) that measures the incorporation of high specific activity [
3H]butan-1-ol into [
3H]phosphatidylbutanol has been developed. The assay has been used to measure PLD activation in human neutrophils and platelets. Both the chemotactic peptide fMet-Leu-Phe and opsonised-zymosan stimulated PLD in the human neutrophil. In the platelet, PLD was stimulated by thrombin and collagen but responses were small and only occurred at high agonist concentrations. This assay has a number of advantages over existing techniques and should be valuable for investigating PLD activation in a variety of isolated cells and possibly intact tissues.</description><subject>1-Butanol</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Blood Platelets - enzymology</subject><subject>Butanols</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydrolases</subject><subject>Kinetics</subject><subject>leukocytes (neutrophilic)</subject><subject>N-Formylmethionine Leucyl-Phenylalanine - pharmacology</subject><subject>Neutrophil</subject><subject>Neutrophils - enzymology</subject><subject>Opsonised-zymosan</subject><subject>PAF, platelet-activating factor (1-O-hexadecyl-2-acetyl-sn-glycerophosphorylcholine)</subject><subject>PBut, phosphatidylbutanol</subject><subject>Phospholipase D</subject><subject>Phospholipase D - analysis</subject><subject>Phospholipase D - blood</subject><subject>Phospholipases - blood</subject><subject>Platelet</subject><subject>platelets</subject><subject>PLD, phospholipase D</subject><subject>Radioisotope Dilution Technique</subject><subject>TLC, thin-layer chromatography</subject><subject>Transphosphatidylation</subject><subject>Tritium</subject><subject>Zymosan - pharmacology</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE9P3DAQxS0EogvtN6CSL63oIdR2Ev-5UO1StkVC6qU9W7YzUV1lk60nu2i_PQ67oreCZMvyvDfP4x8hF5xdccblZ8Z4VdTKlJeGfdJMKVEsjsiMa1UWZSX1MZk9W96QM8Q_LN81N6fkVHAtjTAz8mVO-2ELHXV9QxF6jGPcAnWIbkfbIdH17wHz7uLaIdCvNPZ5jS6MNEDX4Vty0roO4d3hPCe_lrc_b74X9z--3d3M74tQC7UoAg_Su1aoRmnuHPeNlMJ4piSA0GVdBVV76RlozRXUzjdV60vvmqyyNlTlOfm4z12n4e8GcLSriNMErodhg1YZJbVg9YtGXkteSsWysdobQxoQE7R2neLKpZ3lzE6A7UTPTvSsYfYJsF3ktveH_I1fQfPcdCCa9Q8H3WFwXZtcHyL-y85zloLz7FvufQ-xg92r3rbL24WYhKlu2FN1Guh6HwQZ_zZCshgi9AGamCCMthni_3_0CMRlqbg</recordid><startdate>19900507</startdate><enddate>19900507</enddate><creator>Randall, Roger W.</creator><creator>Bonser, Robert W.</creator><creator>Thompson, Neil T.</creator><creator>Garland, Lawrence G.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19900507</creationdate><title>A novel and sensitive assay for phospholipase D in intact cells</title><author>Randall, Roger W. ; Bonser, Robert W. ; Thompson, Neil T. ; Garland, Lawrence G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c527B-c1c6baf27d781aa1bd6629b076ee28354c75b6b0e8817e5abd4fb3bade280fc43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>1-Butanol</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Blood Platelets - enzymology</topic><topic>Butanols</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Hydrolases</topic><topic>Kinetics</topic><topic>leukocytes (neutrophilic)</topic><topic>N-Formylmethionine Leucyl-Phenylalanine - pharmacology</topic><topic>Neutrophil</topic><topic>Neutrophils - enzymology</topic><topic>Opsonised-zymosan</topic><topic>PAF, platelet-activating factor (1-O-hexadecyl-2-acetyl-sn-glycerophosphorylcholine)</topic><topic>PBut, phosphatidylbutanol</topic><topic>Phospholipase D</topic><topic>Phospholipase D - analysis</topic><topic>Phospholipase D - blood</topic><topic>Phospholipases - blood</topic><topic>Platelet</topic><topic>platelets</topic><topic>PLD, phospholipase D</topic><topic>Radioisotope Dilution Technique</topic><topic>TLC, thin-layer chromatography</topic><topic>Transphosphatidylation</topic><topic>Tritium</topic><topic>Zymosan - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Randall, Roger W.</creatorcontrib><creatorcontrib>Bonser, Robert W.</creatorcontrib><creatorcontrib>Thompson, Neil T.</creatorcontrib><creatorcontrib>Garland, Lawrence G.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Randall, Roger W.</au><au>Bonser, Robert W.</au><au>Thompson, Neil T.</au><au>Garland, Lawrence G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel and sensitive assay for phospholipase D in intact cells</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1990-05-07</date><risdate>1990</risdate><volume>264</volume><issue>1</issue><spage>87</spage><epage>90</epage><pages>87-90</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>A novel and sensitive assay for phospholipase D (PLD) that measures the incorporation of high specific activity [
3H]butan-1-ol into [
3H]phosphatidylbutanol has been developed. The assay has been used to measure PLD activation in human neutrophils and platelets. Both the chemotactic peptide fMet-Leu-Phe and opsonised-zymosan stimulated PLD in the human neutrophil. In the platelet, PLD was stimulated by thrombin and collagen but responses were small and only occurred at high agonist concentrations. This assay has a number of advantages over existing techniques and should be valuable for investigating PLD activation in a variety of isolated cells and possibly intact tissues.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2186929</pmid><doi>10.1016/0014-5793(90)80772-B</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | FEBS letters, 1990-05, Vol.264 (1), p.87-90 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | 1-Butanol Analytical, structural and metabolic biochemistry Biological and medical sciences Blood Platelets - enzymology Butanols Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Humans Hydrolases Kinetics leukocytes (neutrophilic) N-Formylmethionine Leucyl-Phenylalanine - pharmacology Neutrophil Neutrophils - enzymology Opsonised-zymosan PAF, platelet-activating factor (1-O-hexadecyl-2-acetyl-sn-glycerophosphorylcholine) PBut, phosphatidylbutanol Phospholipase D Phospholipase D - analysis Phospholipase D - blood Phospholipases - blood Platelet platelets PLD, phospholipase D Radioisotope Dilution Technique TLC, thin-layer chromatography Transphosphatidylation Tritium Zymosan - pharmacology |
| title | A novel and sensitive assay for phospholipase D in intact cells |
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