Villus and crypt cell composition in the secreting mouse jejunum measured with x-ray microanalysis

The response of the villus and crypt cells of the mouse jejunum to secretagogues has been assessed through measurements of cellular composition with x-ray microanalysis. In nonstimulated tissues the Na concentration ([Na]c) of the crypt cells was significantly less, and the K ([K]c) and Cl ([Cl]c) c...

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Veröffentlicht in:The Journal of membrane biology 1998-03, Vol.162 (1), p.17-29
Hauptverfasser: Butt, A G, Bowler, J M, McLaughlin, C W
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Bowler, J M
McLaughlin, C W
description The response of the villus and crypt cells of the mouse jejunum to secretagogues has been assessed through measurements of cellular composition with x-ray microanalysis. In nonstimulated tissues the Na concentration ([Na]c) of the crypt cells was significantly less, and the K ([K]c) and Cl ([Cl]c) concentrations were significantly greater, than that of the villus cells. There was also a decreasing gradient of [Na]c and increasing gradient of [K]c from the villus tip to crypt base due to a greater number of cells with a high [Na]c and low [K]c in the upper regions of the villi. Theophylline (10 mmol L-1) stimulated a sustained increase in bumetanide sensitive short circuit current (Isc) and significantly decreased the [Na]c of the villus cells. Similar, but smaller changes were seen in the crypt cells. Changes in villus cell [Na]c reflected a reduction in the number of cells with a high [Na]c. Inhibition of the apical Na/H exchanger (1 mmol L-1 amiloride) had little effect on basal Isc and the subsequent addition of theophylline increased Isc to a comparable extent as seen without amiloride. However, after amiloride treatment the only change in cellular composition was a reduction in the [Cl]c of both crypt and villus cells, suggesting that both regions are involved in the secretory response. These data suggest that the dominant response of the jejunum to secretagogues is an inhibition of Na absorption via Na/H exchange in the villi and the secretory response is distributed throughout the crypt/villus axis.
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However, after amiloride treatment the only change in cellular composition was a reduction in the [Cl]c of both crypt and villus cells, suggesting that both regions are involved in the secretory response. 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However, after amiloride treatment the only change in cellular composition was a reduction in the [Cl]c of both crypt and villus cells, suggesting that both regions are involved in the secretory response. These data suggest that the dominant response of the jejunum to secretagogues is an inhibition of Na absorption via Na/H exchange in the villi and the secretory response is distributed throughout the crypt/villus axis.</abstract><cop>United States</cop><pmid>9516234</pmid><doi>10.1007/s002329900338</doi><tpages>13</tpages></addata></record>
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subjects Amiloride - pharmacology
Animals
Chlorides - analysis
Electron Probe Microanalysis
Epithelial Cells - chemistry
Freeze Drying
Jejunum - chemistry
Jejunum - drug effects
Jejunum - ultrastructure
Male
Mice
Microtomy
Microvilli - chemistry
Microvilli - ultrastructure
title Villus and crypt cell composition in the secreting mouse jejunum measured with x-ray microanalysis
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