Formation of postsynaptic-like membranes during differentiation of embryonic stem cells in vitro

To analyze the formation of neuromuscular junctions, mouse pluripotent embryonic stem (ES) cells were differentiated via embryoid bodies into skeletal muscle and neuronal cells. The developmentally controlled expression of skeletal muscle-specific genes coding for myf5, myogenin, myoD and myf6, alph...

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Veröffentlicht in:	Experimental cell research 1998-03, Vol.239 (2), p.214-225
Hauptverfasser:	Rohwedel, J, Kleppisch, T, Pich, U, Guan, K, Jin, S, Zuschratter, W, Hopf, C, Hoch, W, Hescheler, J, Witzemann, V, Wobus, A M
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrin - biosynthesis Agrin - genetics Animals Calcium Channels - biosynthesis Calcium Channels - genetics Calcium Channels, L-Type Cell Differentiation Cell Membrane - metabolism Cells, Cultured Embryo, Mammalian - cytology Gene Expression Regulation Membrane Proteins - biosynthesis Membrane Proteins - genetics Mice Microscopy, Fluorescence Muscle Proteins - biosynthesis Muscle Proteins - genetics Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - genetics Neuromuscular Junction - cytology Receptors, Nicotinic - biosynthesis Receptors, Nicotinic - genetics Stem Cells - cytology Stem Cells - metabolism Synaptophysin - biosynthesis Synaptophysin - genetics
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