Reexpression of α-smooth muscle actin isoform in cultured adult rat cardiomyocytes
Expression of α-smooth muscle (sm) actin in regenerating adult cardiomyocytes in culture was investigated. No α-sm-actin could be detected in adult ventricular tissue or in newly dissociated rod-shaped cells, whereas a fraction of the polymorphic flattened out adult cardiac cells in culture did expr...
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| Veröffentlicht in: | Developmental biology 1990-06, Vol.139 (2), p.269-278 |
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| creator | Eppenberger-Eberhardt, Monika Flamme, Ingo Kurer, Vreni Eppenberger, Hans M. |
| description | Expression of α-smooth muscle (sm) actin in regenerating adult cardiomyocytes in culture was investigated. No α-sm-actin could be detected in adult ventricular tissue or in newly dissociated rod-shaped cells, whereas a fraction of the polymorphic flattened out adult cardiac cells in culture did express the protein. Immunofluorescence studies revealed a characteristic staining pattern, suggesting the preferential presence of α-sm-actin in stress fiber-like structures, while newly formed myofibrils contained only little α-sm-actin isoprotein. Cell-cell contacts were resumed, but formation of new gap junctions, as revealed by microinjecting Lucifer yellow, was not dependent on α-sm-actin expression. The behavior corresponds to fetal cardiomyocytes either in tissue or as single cells in culture where expression of α-sm-actin can be observed. Such immunofluorescence staining patterns with corresponding immunoblot data can be expected when a return to a less differentiated, more fetal state of the adult cardiomyocyte in culture is assumed. The possible role of the α-sm-actin and α-sarcomeric actin isoforms during reformation of myofibrillar sarcomeres is discussed. |
| doi_str_mv | 10.1016/0012-1606(90)90296-U |
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No α-sm-actin could be detected in adult ventricular tissue or in newly dissociated rod-shaped cells, whereas a fraction of the polymorphic flattened out adult cardiac cells in culture did express the protein. Immunofluorescence studies revealed a characteristic staining pattern, suggesting the preferential presence of α-sm-actin in stress fiber-like structures, while newly formed myofibrils contained only little α-sm-actin isoprotein. Cell-cell contacts were resumed, but formation of new gap junctions, as revealed by microinjecting Lucifer yellow, was not dependent on α-sm-actin expression. The behavior corresponds to fetal cardiomyocytes either in tissue or as single cells in culture where expression of α-sm-actin can be observed. Such immunofluorescence staining patterns with corresponding immunoblot data can be expected when a return to a less differentiated, more fetal state of the adult cardiomyocyte in culture is assumed. 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No α-sm-actin could be detected in adult ventricular tissue or in newly dissociated rod-shaped cells, whereas a fraction of the polymorphic flattened out adult cardiac cells in culture did express the protein. Immunofluorescence studies revealed a characteristic staining pattern, suggesting the preferential presence of α-sm-actin in stress fiber-like structures, while newly formed myofibrils contained only little α-sm-actin isoprotein. Cell-cell contacts were resumed, but formation of new gap junctions, as revealed by microinjecting Lucifer yellow, was not dependent on α-sm-actin expression. The behavior corresponds to fetal cardiomyocytes either in tissue or as single cells in culture where expression of α-sm-actin can be observed. Such immunofluorescence staining patterns with corresponding immunoblot data can be expected when a return to a less differentiated, more fetal state of the adult cardiomyocyte in culture is assumed. The possible role of the α-sm-actin and α-sarcomeric actin isoforms during reformation of myofibrillar sarcomeres is discussed.</description><subject>Actins - analysis</subject><subject>Actins - biosynthesis</subject><subject>Actins - isolation & purification</subject><subject>alpha -actin</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Antigen-Antibody Complex - analysis</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Degeneration. Regeneration. Wound healing. Graft</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heart Ventricles - cytology</subject><subject>Heart Ventricles - metabolism</subject><subject>Immunoblotting</subject><subject>Molecular Weight</subject><subject>Myocardium - cytology</subject><subject>Myocardium - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMGKFDEQhoMo6-zqGyjkIIseWlNJd9K5CLK4KiwsqAPeQiZdjZHuzpjqlp3H2hfxmcw4wxz1VAX_9xfFx9gzEK9BgH4jBMgKtNAvrXhlhbS6Wj9gKxC2qRpdf3vIVifkMTsn-iGEUG2rztiZhFbbWq3Yl8-Id9uMRDFNPPX8931FY0rzdz4uFAbkPsxx4pFSn_LIyxqWYV4ydtx3ZePZzzz43MU07lLYzUhP2KPeD4RPj_OCra_ff736WN3cfvh09e6mCjWYuaohtKh1rxthQGslm1Z20oJEML1vUAivdI9t7TtUtTQYpNlsVIlV0GC0umCXh7vbnH4uSLMbIwUcBj9hWsgZWyCt4L8gNMZAC3uwPoAhJ6KMvdvmOPq8cyDcXrrbG3V7o84K91e6W5fa8-P9ZTNidyodLZf8xTH3FPzQZz-FSCesMK20TcHeHjAs0n5FzI5CxClgFzOG2XUp_vuPP8hXniY</recordid><startdate>19900601</startdate><enddate>19900601</enddate><creator>Eppenberger-Eberhardt, Monika</creator><creator>Flamme, Ingo</creator><creator>Kurer, Vreni</creator><creator>Eppenberger, Hans M.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19900601</creationdate><title>Reexpression of α-smooth muscle actin isoform in cultured adult rat cardiomyocytes</title><author>Eppenberger-Eberhardt, Monika ; Flamme, Ingo ; Kurer, Vreni ; Eppenberger, Hans M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-41c8e66f650716632582d2912e17fa5e00a36fe84ade3427ec27bb32e13c61763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Actins - analysis</topic><topic>Actins - biosynthesis</topic><topic>Actins - isolation & purification</topic><topic>alpha -actin</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antigen-Antibody Complex - analysis</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Degeneration. Regeneration. Wound healing. Graft</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heart Ventricles - cytology</topic><topic>Heart Ventricles - metabolism</topic><topic>Immunoblotting</topic><topic>Molecular Weight</topic><topic>Myocardium - cytology</topic><topic>Myocardium - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eppenberger-Eberhardt, Monika</creatorcontrib><creatorcontrib>Flamme, Ingo</creatorcontrib><creatorcontrib>Kurer, Vreni</creatorcontrib><creatorcontrib>Eppenberger, Hans M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eppenberger-Eberhardt, Monika</au><au>Flamme, Ingo</au><au>Kurer, Vreni</au><au>Eppenberger, Hans M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reexpression of α-smooth muscle actin isoform in cultured adult rat cardiomyocytes</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>1990-06-01</date><risdate>1990</risdate><volume>139</volume><issue>2</issue><spage>269</spage><epage>278</epage><pages>269-278</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><coden>DEBIAO</coden><abstract>Expression of α-smooth muscle (sm) actin in regenerating adult cardiomyocytes in culture was investigated. No α-sm-actin could be detected in adult ventricular tissue or in newly dissociated rod-shaped cells, whereas a fraction of the polymorphic flattened out adult cardiac cells in culture did express the protein. Immunofluorescence studies revealed a characteristic staining pattern, suggesting the preferential presence of α-sm-actin in stress fiber-like structures, while newly formed myofibrils contained only little α-sm-actin isoprotein. Cell-cell contacts were resumed, but formation of new gap junctions, as revealed by microinjecting Lucifer yellow, was not dependent on α-sm-actin expression. The behavior corresponds to fetal cardiomyocytes either in tissue or as single cells in culture where expression of α-sm-actin can be observed. Such immunofluorescence staining patterns with corresponding immunoblot data can be expected when a return to a less differentiated, more fetal state of the adult cardiomyocyte in culture is assumed. The possible role of the α-sm-actin and α-sarcomeric actin isoforms during reformation of myofibrillar sarcomeres is discussed.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>2186943</pmid><doi>10.1016/0012-1606(90)90296-U</doi><tpages>10</tpages></addata></record> |
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| subjects | Actins - analysis Actins - biosynthesis Actins - isolation & purification alpha -actin Animals Antibodies, Monoclonal Antigen-Antibody Complex - analysis Biological and medical sciences Cells, Cultured Degeneration. Regeneration. Wound healing. Graft Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Heart Ventricles - cytology Heart Ventricles - metabolism Immunoblotting Molecular Weight Myocardium - cytology Myocardium - metabolism Rats Rats, Inbred Strains Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Reexpression of α-smooth muscle actin isoform in cultured adult rat cardiomyocytes |
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