Molecular cloning and mRNA localization of tomato pollen profilin
The actin cytoskeleton plays an important role in the growth of pollen tube. The actin-binding protein profilin could play a role in regulating the organization of the actin filaments. Using the RT-PCR technique, we isolated a cDNA clone (designated LePro 1) encoding profilin from pollen grains of t...
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| Veröffentlicht in: | Plant molecular biology 1998-03, Vol.36 (5), p.699-707 |
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| creator | Yu, L.X. (Cornell Univ., Ithaca, NY (USA). Section of Plant Biology) Nasrallah, J Valenta, R Parthasarathy, M.V |
| description | The actin cytoskeleton plays an important role in the growth of pollen tube. The actin-binding protein profilin could play a role in regulating the organization of the actin filaments. Using the RT-PCR technique, we isolated a cDNA clone (designated LePro 1) encoding profilin from pollen grains of tomato (Lycopersicon esculentum Mill. cv. Moneymaker). Sequence analysis of the insert shows 87% similarity to tobacco ntPro2, 78% to timothy grass profilin, 77% to Arabidopsis AthPRF4, 77% to maize ZmPro3, and 73% to birch profilin. Both quantitative PCR and RNA gel blot analyses demonstrated that LePro 1 is expressed in a tissue- or cell-type specific manner in the tomato plant. In situ hybridization of 2 microns thick anther sections using a non-radioactive labeling method reveals that LePro 1 is expressed only in pollen grains, with undetectable transcription in other parts of the anther or the other organs. Phylogenetic analysis of amino acid sequences of 18 plant profilins indicates that two distinct profilin gene classes are present in higher plants. One is pollen-specific, another is constitutive. LePro 1 belongs to the former class. |
| doi_str_mv | 10.1023/A:1005971327353 |
| format | Article |
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(Cornell Univ., Ithaca, NY (USA). Section of Plant Biology) ; Nasrallah, J ; Valenta, R ; Parthasarathy, M.V</creator><creatorcontrib>Yu, L.X. (Cornell Univ., Ithaca, NY (USA). Section of Plant Biology) ; Nasrallah, J ; Valenta, R ; Parthasarathy, M.V</creatorcontrib><description>The actin cytoskeleton plays an important role in the growth of pollen tube. The actin-binding protein profilin could play a role in regulating the organization of the actin filaments. Using the RT-PCR technique, we isolated a cDNA clone (designated LePro 1) encoding profilin from pollen grains of tomato (Lycopersicon esculentum Mill. cv. Moneymaker). Sequence analysis of the insert shows 87% similarity to tobacco ntPro2, 78% to timothy grass profilin, 77% to Arabidopsis AthPRF4, 77% to maize ZmPro3, and 73% to birch profilin. Both quantitative PCR and RNA gel blot analyses demonstrated that LePro 1 is expressed in a tissue- or cell-type specific manner in the tomato plant. In situ hybridization of 2 microns thick anther sections using a non-radioactive labeling method reveals that LePro 1 is expressed only in pollen grains, with undetectable transcription in other parts of the anther or the other organs. Phylogenetic analysis of amino acid sequences of 18 plant profilins indicates that two distinct profilin gene classes are present in higher plants. One is pollen-specific, another is constitutive. LePro 1 belongs to the former class.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1023/A:1005971327353</identifier><identifier>PMID: 9526502</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Amino Acid Sequence ; Amino acids ; Arabidopsis Proteins ; ARN MENSAJERO ; ARN MESSAGER ; Base Sequence ; CLONACION MOLECULAR ; CLONAGE MOLECULAIRE ; Cloning ; Cloning, Molecular ; Contractile Proteins ; Cytoskeleton ; DNA HYBRIDIZATION ; DNA Primers - genetics ; DNA, Complementary - genetics ; DNA, Complementary - isolation & purification ; DNA, Plant - genetics ; DNA, Plant - isolation & purification ; EXPRESION GENICA ; EXPRESSION DES GENES ; GENE EXPRESSION ; Genes, Plant ; HIBRIDACION DE ADN ; HYBRIDATION D'ADN ; In Situ Hybridization ; LYCOPERSICON ESCULENTUM ; Lycopersicon esculentum - genetics ; Lycopersicon esculentum - growth & development ; Lycopersicon esculentum - metabolism ; MESSENGER RNA ; Microfilament Proteins - genetics ; MOLECULAR CLONING ; Molecular Sequence Data ; NUCLEOTIDE SEQUENCE ; Phylogeny ; POLEN ; POLLEN ; Pollen - genetics ; Pollen - growth & development ; Polymerase Chain Reaction ; Profilins ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA, Plant - genetics ; RNA, Plant - metabolism ; SECUENCIA NUCLEOTIDICA ; Sequence Homology, Amino Acid ; SEQUENCE NUCLEOTIDIQUE ; Species Specificity ; Tomatoes</subject><ispartof>Plant molecular biology, 1998-03, Vol.36 (5), p.699-707</ispartof><rights>Kluwer Academic Publishers 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-645fcd2fb3809695af4c0b1ccc677f39daab7d8afddaf9845c04273dc6a5f7e43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9526502$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, L.X. (Cornell Univ., Ithaca, NY (USA). Section of Plant Biology)</creatorcontrib><creatorcontrib>Nasrallah, J</creatorcontrib><creatorcontrib>Valenta, R</creatorcontrib><creatorcontrib>Parthasarathy, M.V</creatorcontrib><title>Molecular cloning and mRNA localization of tomato pollen profilin</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>The actin cytoskeleton plays an important role in the growth of pollen tube. The actin-binding protein profilin could play a role in regulating the organization of the actin filaments. Using the RT-PCR technique, we isolated a cDNA clone (designated LePro 1) encoding profilin from pollen grains of tomato (Lycopersicon esculentum Mill. cv. Moneymaker). Sequence analysis of the insert shows 87% similarity to tobacco ntPro2, 78% to timothy grass profilin, 77% to Arabidopsis AthPRF4, 77% to maize ZmPro3, and 73% to birch profilin. Both quantitative PCR and RNA gel blot analyses demonstrated that LePro 1 is expressed in a tissue- or cell-type specific manner in the tomato plant. In situ hybridization of 2 microns thick anther sections using a non-radioactive labeling method reveals that LePro 1 is expressed only in pollen grains, with undetectable transcription in other parts of the anther or the other organs. Phylogenetic analysis of amino acid sequences of 18 plant profilins indicates that two distinct profilin gene classes are present in higher plants. One is pollen-specific, another is constitutive. LePro 1 belongs to the former class.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Arabidopsis Proteins</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>Base Sequence</subject><subject>CLONACION MOLECULAR</subject><subject>CLONAGE MOLECULAIRE</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>Contractile Proteins</subject><subject>Cytoskeleton</subject><subject>DNA HYBRIDIZATION</subject><subject>DNA Primers - genetics</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Complementary - isolation & purification</subject><subject>DNA, Plant - genetics</subject><subject>DNA, Plant - isolation & purification</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>GENE EXPRESSION</subject><subject>Genes, Plant</subject><subject>HIBRIDACION DE ADN</subject><subject>HYBRIDATION D'ADN</subject><subject>In Situ Hybridization</subject><subject>LYCOPERSICON ESCULENTUM</subject><subject>Lycopersicon esculentum - genetics</subject><subject>Lycopersicon esculentum - growth & development</subject><subject>Lycopersicon esculentum - metabolism</subject><subject>MESSENGER RNA</subject><subject>Microfilament Proteins - genetics</subject><subject>MOLECULAR CLONING</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>Phylogeny</subject><subject>POLEN</subject><subject>POLLEN</subject><subject>Pollen - genetics</subject><subject>Pollen - growth & development</subject><subject>Polymerase Chain Reaction</subject><subject>Profilins</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Plant - genetics</subject><subject>RNA, Plant - metabolism</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>Sequence Homology, Amino Acid</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>Species Specificity</subject><subject>Tomatoes</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkDtLBDEUhYMo67paWwmDhd1o3pnYDYsvWFcQrYdMHkskk6zzKPTXG3FBsLG6xf04h_MBcIrgJYKYXNXXCEImBSJYEEb2wBwxQUoGcbUP5hBxUVKK8CE4GoY3CDNM-AzMJMM8M3NQP6Zg9RRUX-iQoo-bQkVTdM_rughJq-A_1ehTLJIrxtSpMRXbFIKNxbZPzgcfj8GBU2GwJ7u7AK-3Ny_L-3L1dPewrFelJgSPJafMaYNdSyoouWTKUQ1bpLXmQjgijVKtMJVyxignK8o0pHmS0VwxJywlC3Dxk5uL3yc7jE3nB21DUNGmaWiEFBwRLv8FEceVZPA78fwP-JamPuYRjRCIZj-EZOhsB01tZ02z7X2n-o9mZ_D371Rq1Kb3Q7NeISmrbFoISr4AbjZ6lw</recordid><startdate>199803</startdate><enddate>199803</enddate><creator>Yu, L.X. 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Section of Plant Biology) ; Nasrallah, J ; Valenta, R ; Parthasarathy, M.V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-645fcd2fb3809695af4c0b1ccc677f39daab7d8afddaf9845c04273dc6a5f7e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Arabidopsis Proteins</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>Base Sequence</topic><topic>CLONACION MOLECULAR</topic><topic>CLONAGE MOLECULAIRE</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>Contractile Proteins</topic><topic>Cytoskeleton</topic><topic>DNA HYBRIDIZATION</topic><topic>DNA Primers - genetics</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Complementary - isolation & purification</topic><topic>DNA, Plant - genetics</topic><topic>DNA, Plant - isolation & purification</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>GENE EXPRESSION</topic><topic>Genes, Plant</topic><topic>HIBRIDACION DE ADN</topic><topic>HYBRIDATION D'ADN</topic><topic>In Situ Hybridization</topic><topic>LYCOPERSICON ESCULENTUM</topic><topic>Lycopersicon esculentum - genetics</topic><topic>Lycopersicon esculentum - growth & development</topic><topic>Lycopersicon esculentum - metabolism</topic><topic>MESSENGER RNA</topic><topic>Microfilament Proteins - genetics</topic><topic>MOLECULAR CLONING</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>Phylogeny</topic><topic>POLEN</topic><topic>POLLEN</topic><topic>Pollen - genetics</topic><topic>Pollen - growth & development</topic><topic>Polymerase Chain Reaction</topic><topic>Profilins</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Plant - genetics</topic><topic>RNA, Plant - metabolism</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>Sequence Homology, Amino Acid</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>Species Specificity</topic><topic>Tomatoes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, L.X. 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(Cornell Univ., Ithaca, NY (USA). Section of Plant Biology)</au><au>Nasrallah, J</au><au>Valenta, R</au><au>Parthasarathy, M.V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and mRNA localization of tomato pollen profilin</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1998-03</date><risdate>1998</risdate><volume>36</volume><issue>5</issue><spage>699</spage><epage>707</epage><pages>699-707</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>The actin cytoskeleton plays an important role in the growth of pollen tube. The actin-binding protein profilin could play a role in regulating the organization of the actin filaments. Using the RT-PCR technique, we isolated a cDNA clone (designated LePro 1) encoding profilin from pollen grains of tomato (Lycopersicon esculentum Mill. cv. Moneymaker). Sequence analysis of the insert shows 87% similarity to tobacco ntPro2, 78% to timothy grass profilin, 77% to Arabidopsis AthPRF4, 77% to maize ZmPro3, and 73% to birch profilin. Both quantitative PCR and RNA gel blot analyses demonstrated that LePro 1 is expressed in a tissue- or cell-type specific manner in the tomato plant. In situ hybridization of 2 microns thick anther sections using a non-radioactive labeling method reveals that LePro 1 is expressed only in pollen grains, with undetectable transcription in other parts of the anther or the other organs. Phylogenetic analysis of amino acid sequences of 18 plant profilins indicates that two distinct profilin gene classes are present in higher plants. One is pollen-specific, another is constitutive. LePro 1 belongs to the former class.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>9526502</pmid><doi>10.1023/A:1005971327353</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Plant molecular biology, 1998-03, Vol.36 (5), p.699-707 |
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| subjects | Amino Acid Sequence Amino acids Arabidopsis Proteins ARN MENSAJERO ARN MESSAGER Base Sequence CLONACION MOLECULAR CLONAGE MOLECULAIRE Cloning Cloning, Molecular Contractile Proteins Cytoskeleton DNA HYBRIDIZATION DNA Primers - genetics DNA, Complementary - genetics DNA, Complementary - isolation & purification DNA, Plant - genetics DNA, Plant - isolation & purification EXPRESION GENICA EXPRESSION DES GENES GENE EXPRESSION Genes, Plant HIBRIDACION DE ADN HYBRIDATION D'ADN In Situ Hybridization LYCOPERSICON ESCULENTUM Lycopersicon esculentum - genetics Lycopersicon esculentum - growth & development Lycopersicon esculentum - metabolism MESSENGER RNA Microfilament Proteins - genetics MOLECULAR CLONING Molecular Sequence Data NUCLEOTIDE SEQUENCE Phylogeny POLEN POLLEN Pollen - genetics Pollen - growth & development Polymerase Chain Reaction Profilins RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Plant - genetics RNA, Plant - metabolism SECUENCIA NUCLEOTIDICA Sequence Homology, Amino Acid SEQUENCE NUCLEOTIDIQUE Species Specificity Tomatoes |
| title | Molecular cloning and mRNA localization of tomato pollen profilin |
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