Interaction of host cell proteins with the human T-cell leukemia virus type I transcriptional control region. II. A comprehensive map of protein-binding sites facilitates construction of a simple chimeric promoter responsive to the viral tax2 gene product
We present a map describing the binding of cellular proteins to a 300-base pair (bp) region of the human T-cell leukemia virus type I (HTLV-I) long terminal repeat. The map accounts for nearly all of the DNase I protection reported in a previous study using crude nuclear extracts. Notable features i...
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| Veröffentlicht in: | The Journal of biological chemistry 1990-05, Vol.265 (14), p.8237-8242 |
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| container_title | The Journal of biological chemistry |
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| creator | NYBORG, J. K MATTHEWS, M.-A. H YUCEL, J WALLS, L GOLDE, W. T DYNAN, W. S WACHSMAN, W |
| description | We present a map describing the binding of cellular proteins to a 300-base pair (bp) region of the human T-cell leukemia virus
type I (HTLV-I) long terminal repeat. The map accounts for nearly all of the DNase I protection reported in a previous study
using crude nuclear extracts. Notable features include a complex arrangement of overlapping binding sites encompassing the
21-bp repeat elements (see accompanying paper) as well as binding sites for the transcription factors Sp1 and NF-I that significantly
deviate from the previously defined consensus recognition sequences. Based on the binding results, we constructed simple chimeric
promoters containing 21-bp repeat elements, Sp1-, and nuclear factor I-binding sites upstream of a TATA box. Transient transfection
experiments show that these promoters are expressed in T-cells and are regulated by the viral tax2 gene product. Deletion
of the Sp1 and nuclear factor I sites abolishes tax-induction, suggesting that one or both of these proteins play a role in
mediating the tax-responsiveness conferred by the 21-bp repeat element. |
| doi_str_mv | 10.1016/S0021-9258(19)39063-5 |
| format | Article |
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type I (HTLV-I) long terminal repeat. The map accounts for nearly all of the DNase I protection reported in a previous study
using crude nuclear extracts. Notable features include a complex arrangement of overlapping binding sites encompassing the
21-bp repeat elements (see accompanying paper) as well as binding sites for the transcription factors Sp1 and NF-I that significantly
deviate from the previously defined consensus recognition sequences. Based on the binding results, we constructed simple chimeric
promoters containing 21-bp repeat elements, Sp1-, and nuclear factor I-binding sites upstream of a TATA box. Transient transfection
experiments show that these promoters are expressed in T-cells and are regulated by the viral tax2 gene product. Deletion
of the Sp1 and nuclear factor I sites abolishes tax-induction, suggesting that one or both of these proteins play a role in
mediating the tax-responsiveness conferred by the 21-bp repeat element.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(19)39063-5</identifier><identifier>PMID: 2186038</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>AIDS/HIV ; Base Sequence ; Binding Sites ; Biological and medical sciences ; CCAAT-Enhancer-Binding Proteins ; Cell Line ; Cloning, Molecular ; Deoxyribonuclease I ; DNA-Binding Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Viral ; Genes, Viral ; HeLa Cells ; Human T-lymphotropic virus 1 - genetics ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; NFI Transcription Factors ; Nuclear Proteins ; Promoter Regions, Genetic - genetics ; Repetitive Sequences, Nucleic Acid ; Sp1 Transcription Factor ; T-Lymphocytes - metabolism ; T-Lymphocytes - microbiology ; Trans-Activators - physiology ; Transcription Factors - metabolism ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. Rna processing ; Transfection ; Y-Box-Binding Protein 1</subject><ispartof>The Journal of biological chemistry, 1990-05, Vol.265 (14), p.8237-8242</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3245-5c1e8c64e67fe925664521869d2543749e48aacf5f8a8e2c91f25f18c587e80c3</citedby><cites>FETCH-LOGICAL-c3245-5c1e8c64e67fe925664521869d2543749e48aacf5f8a8e2c91f25f18c587e80c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19747879$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2186038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NYBORG, J. K</creatorcontrib><creatorcontrib>MATTHEWS, M.-A. H</creatorcontrib><creatorcontrib>YUCEL, J</creatorcontrib><creatorcontrib>WALLS, L</creatorcontrib><creatorcontrib>GOLDE, W. T</creatorcontrib><creatorcontrib>DYNAN, W. S</creatorcontrib><creatorcontrib>WACHSMAN, W</creatorcontrib><title>Interaction of host cell proteins with the human T-cell leukemia virus type I transcriptional control region. II. A comprehensive map of protein-binding sites facilitates construction of a simple chimeric promoter responsive to the viral tax2 gene product</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>We present a map describing the binding of cellular proteins to a 300-base pair (bp) region of the human T-cell leukemia virus
type I (HTLV-I) long terminal repeat. The map accounts for nearly all of the DNase I protection reported in a previous study
using crude nuclear extracts. Notable features include a complex arrangement of overlapping binding sites encompassing the
21-bp repeat elements (see accompanying paper) as well as binding sites for the transcription factors Sp1 and NF-I that significantly
deviate from the previously defined consensus recognition sequences. Based on the binding results, we constructed simple chimeric
promoters containing 21-bp repeat elements, Sp1-, and nuclear factor I-binding sites upstream of a TATA box. Transient transfection
experiments show that these promoters are expressed in T-cells and are regulated by the viral tax2 gene product. Deletion
of the Sp1 and nuclear factor I sites abolishes tax-induction, suggesting that one or both of these proteins play a role in
mediating the tax-responsiveness conferred by the 21-bp repeat element.</description><subject>AIDS/HIV</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>CCAAT-Enhancer-Binding Proteins</subject><subject>Cell Line</subject><subject>Cloning, Molecular</subject><subject>Deoxyribonuclease I</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Viral</subject><subject>Genes, Viral</subject><subject>HeLa Cells</subject><subject>Human T-lymphotropic virus 1 - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>NFI Transcription Factors</subject><subject>Nuclear Proteins</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Sp1 Transcription Factor</subject><subject>T-Lymphocytes - metabolism</subject><subject>T-Lymphocytes - microbiology</subject><subject>Trans-Activators - physiology</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>Transfection</subject><subject>Y-Box-Binding Protein 1</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU9v1DAQxSMEKkvhI1QaCYHgkCV24sQ-VhV_VqrEgSJxs7zeycYQ28F2WvrpwdldLb7Ymnn-vdG8orgi1ZpUpP3wraooKQVl_B0R72tRtXXJnhQrUvG6rBn58bRYnSXPixcx_qzyaQS5KC4o4W1V81Xxd-MSBqWT8Q58D4OPCTSOI0zBJzQuwoNJA6QBYZitcnBXHtojzr_QGgX3JswR0uOEsIEUlIs6mGnhqRG0dyn4EQLuc2ENm80arnPVTgEHdNHcI1g1Lc4nv3Jr3M64PUSTMEKvtBlNUss7w2IK83lWlTV2GhH0YCwGoxeGzZSQ_eLkj_jkD8PnMfM8Sf2hsEeHi3SXUS-LZ70aI7463ZfF908f726-lLdfP29urm9LXdOGlUwT5LptsO16zAtt24YtOxQ7ypq6awQ2XCnds54rjlQL0lPWE64Z75BXur4s3h652ff3jDFJa-KySOXQz1F2omsJETQL2VGog48xYC-nYKwKj5JUcgleHoKXS6qSCHkIXrL87-pkMG8t7s6_Tknn_ptTX0Wtxj4HpU38Dxdd0_FOZN3ro24w--HBBJRb4_WAVtKWSdJITuuu_gd7P8h2</recordid><startdate>19900515</startdate><enddate>19900515</enddate><creator>NYBORG, J. K</creator><creator>MATTHEWS, M.-A. H</creator><creator>YUCEL, J</creator><creator>WALLS, L</creator><creator>GOLDE, W. T</creator><creator>DYNAN, W. S</creator><creator>WACHSMAN, W</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900515</creationdate><title>Interaction of host cell proteins with the human T-cell leukemia virus type I transcriptional control region. II. A comprehensive map of protein-binding sites facilitates construction of a simple chimeric promoter responsive to the viral tax2 gene product</title><author>NYBORG, J. K ; MATTHEWS, M.-A. H ; YUCEL, J ; WALLS, L ; GOLDE, W. T ; DYNAN, W. S ; WACHSMAN, W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3245-5c1e8c64e67fe925664521869d2543749e48aacf5f8a8e2c91f25f18c587e80c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>AIDS/HIV</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>CCAAT-Enhancer-Binding Proteins</topic><topic>Cell Line</topic><topic>Cloning, Molecular</topic><topic>Deoxyribonuclease I</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Viral</topic><topic>Genes, Viral</topic><topic>HeLa Cells</topic><topic>Human T-lymphotropic virus 1 - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>NFI Transcription Factors</topic><topic>Nuclear Proteins</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Sp1 Transcription Factor</topic><topic>T-Lymphocytes - metabolism</topic><topic>T-Lymphocytes - microbiology</topic><topic>Trans-Activators - physiology</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Transfection</topic><topic>Y-Box-Binding Protein 1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NYBORG, J. K</creatorcontrib><creatorcontrib>MATTHEWS, M.-A. H</creatorcontrib><creatorcontrib>YUCEL, J</creatorcontrib><creatorcontrib>WALLS, L</creatorcontrib><creatorcontrib>GOLDE, W. T</creatorcontrib><creatorcontrib>DYNAN, W. S</creatorcontrib><creatorcontrib>WACHSMAN, W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NYBORG, J. K</au><au>MATTHEWS, M.-A. H</au><au>YUCEL, J</au><au>WALLS, L</au><au>GOLDE, W. T</au><au>DYNAN, W. S</au><au>WACHSMAN, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction of host cell proteins with the human T-cell leukemia virus type I transcriptional control region. II. A comprehensive map of protein-binding sites facilitates construction of a simple chimeric promoter responsive to the viral tax2 gene product</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1990-05-15</date><risdate>1990</risdate><volume>265</volume><issue>14</issue><spage>8237</spage><epage>8242</epage><pages>8237-8242</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>We present a map describing the binding of cellular proteins to a 300-base pair (bp) region of the human T-cell leukemia virus
type I (HTLV-I) long terminal repeat. The map accounts for nearly all of the DNase I protection reported in a previous study
using crude nuclear extracts. Notable features include a complex arrangement of overlapping binding sites encompassing the
21-bp repeat elements (see accompanying paper) as well as binding sites for the transcription factors Sp1 and NF-I that significantly
deviate from the previously defined consensus recognition sequences. Based on the binding results, we constructed simple chimeric
promoters containing 21-bp repeat elements, Sp1-, and nuclear factor I-binding sites upstream of a TATA box. Transient transfection
experiments show that these promoters are expressed in T-cells and are regulated by the viral tax2 gene product. Deletion
of the Sp1 and nuclear factor I sites abolishes tax-induction, suggesting that one or both of these proteins play a role in
mediating the tax-responsiveness conferred by the 21-bp repeat element.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>2186038</pmid><doi>10.1016/S0021-9258(19)39063-5</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1990-05, Vol.265 (14), p.8237-8242 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79761192 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | AIDS/HIV Base Sequence Binding Sites Biological and medical sciences CCAAT-Enhancer-Binding Proteins Cell Line Cloning, Molecular Deoxyribonuclease I DNA-Binding Proteins - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Viral Genes, Viral HeLa Cells Human T-lymphotropic virus 1 - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data NFI Transcription Factors Nuclear Proteins Promoter Regions, Genetic - genetics Repetitive Sequences, Nucleic Acid Sp1 Transcription Factor T-Lymphocytes - metabolism T-Lymphocytes - microbiology Trans-Activators - physiology Transcription Factors - metabolism Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing Transfection Y-Box-Binding Protein 1 |
| title | Interaction of host cell proteins with the human T-cell leukemia virus type I transcriptional control region. II. A comprehensive map of protein-binding sites facilitates construction of a simple chimeric promoter responsive to the viral tax2 gene product |
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