Effects of tissue absorbance on NAD(P)H and Indo-1 fluorescence from perfused rabbit hearts
The effects of tissue optical absorbance on intracellular NAD(P)H and Indo-1 fluorescence emission have been evaluated in the perfused rabbit heart. These results demonstrate that the tissue optical absorbance significantly modifies the emission characteristics of these fluorophores. This tissue ‘in...
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| Veröffentlicht in: | FEBS letters 1990-03, Vol.262 (2), p.287-292 |
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| creator | Fralix, Teresa A. Heineman, Frederick W. Balaban, Robert S. |
| description | The effects of tissue optical absorbance on intracellular NAD(P)H and Indo-1 fluorescence emission have been evaluated in the perfused rabbit heart. These results demonstrate that the tissue optical absorbance significantly modifies the emission characteristics of these fluorophores. This tissue ‘inner filter’ effect, observed with both probes, changed as a function of tissue oxygenation and redox state in a wavelength-dependent manner. Pathlength calculations from these results indicate that this inner filter effect could occur with a mean pathlength of 310 μm due to the extremely high extinction coefficient of heart tissue. It is concluded that tissue optical absorbance significantly affects the fluorescent emission characteristics of both intrinsic and extrinsic probes in the intact heart, under a variety of conditions. Several potential methods of correcting for these tissue inner filter effects are discussed. |
| doi_str_mv | 10.1016/0014-5793(90)80212-2 |
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These results demonstrate that the tissue optical absorbance significantly modifies the emission characteristics of these fluorophores. This tissue ‘inner filter’ effect, observed with both probes, changed as a function of tissue oxygenation and redox state in a wavelength-dependent manner. Pathlength calculations from these results indicate that this inner filter effect could occur with a mean pathlength of 310 μm due to the extremely high extinction coefficient of heart tissue. It is concluded that tissue optical absorbance significantly affects the fluorescent emission characteristics of both intrinsic and extrinsic probes in the intact heart, under a variety of conditions. 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Biooptics ; Optical spectroscopy ; Perfusion ; Rabbits ; Spectrofluorometry ; Spectrometry, Fluorescence - methods ; Tissues, organs and organisms biophysics</subject><ispartof>FEBS letters, 1990-03, Vol.262 (2), p.287-292</ispartof><rights>1990</rights><rights>FEBS Letters 262 (1990) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4112-8498e7cd8c134aae226ff9edf39a5d7a89af1200146994253d4ed732d576ae83</citedby><cites>FETCH-LOGICAL-c4112-8498e7cd8c134aae226ff9edf39a5d7a89af1200146994253d4ed732d576ae83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(90)80212-2$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19771452$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2335209$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fralix, Teresa A.</creatorcontrib><creatorcontrib>Heineman, Frederick W.</creatorcontrib><creatorcontrib>Balaban, Robert S.</creatorcontrib><title>Effects of tissue absorbance on NAD(P)H and Indo-1 fluorescence from perfused rabbit hearts</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The effects of tissue optical absorbance on intracellular NAD(P)H and Indo-1 fluorescence emission have been evaluated in the perfused rabbit heart. These results demonstrate that the tissue optical absorbance significantly modifies the emission characteristics of these fluorophores. This tissue ‘inner filter’ effect, observed with both probes, changed as a function of tissue oxygenation and redox state in a wavelength-dependent manner. Pathlength calculations from these results indicate that this inner filter effect could occur with a mean pathlength of 310 μm due to the extremely high extinction coefficient of heart tissue. It is concluded that tissue optical absorbance significantly affects the fluorescent emission characteristics of both intrinsic and extrinsic probes in the intact heart, under a variety of conditions. Several potential methods of correcting for these tissue inner filter effects are discussed.</description><subject>A23187</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological effects of radiation</subject><subject>Calcium</subject><subject>Cyanides - pharmacology</subject><subject>Cytochrome</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heart - drug effects</subject><subject>Heart - physiology</subject><subject>Hypoxia</subject><subject>Hypoxia - pathology</subject><subject>In Vitro Techniques</subject><subject>Indoles</subject><subject>Inner filter</subject><subject>Myoglobin</subject><subject>NADH</subject><subject>NADP</subject><subject>Non ionizing radiations. Hertzian waves. Biooptics</subject><subject>Optical spectroscopy</subject><subject>Perfusion</subject><subject>Rabbits</subject><subject>Spectrofluorometry</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Tissues, organs and organisms biophysics</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EKkvhH4DkC6g9BPyRxPYFqZRdWqkCDr1xsBx7LIyy8WInoP577N1Ve6s4WZ5555mZdxB6Tcl7Smj_gRDaNp1Q_EyRc0kYZQ17glZUCt7wtpdP0epe8hy9yPkXKX9J1Qk6YZx3jKgV-rH2HuyccfR4DjkvgM2QYxrMZAHHCX-9-Hz2_fwKm8nh68nFhmI_LjFBtlAlPsUt3kHySwaHkxmGMOOfYNKcX6Jn3owZXh3fU3S7Wd9eXjU3375cX17cNLalZWjZKgnCOmkpb40BxnrvFTjPlemcMFIZT1ldpVeqZR13LTjBmetEb0DyU_TugN2l-HuBPOttKMONo5kgLlkLJTpBSFeE7UFoU8w5gde7FLYm3WlKdLVU1ya6-qUV0XtLNStlb478ZdiCuy86eljyb495k60ZfSrWhfzAVkLQtquczUH3N4xw91-99Wb9ab95jSuyj1bQxwMIiql_AiSdbajHcCGVW2oXw-Mb_QMyYqPY</recordid><startdate>19900326</startdate><enddate>19900326</enddate><creator>Fralix, Teresa A.</creator><creator>Heineman, Frederick W.</creator><creator>Balaban, Robert S.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900326</creationdate><title>Effects of tissue absorbance on NAD(P)H and Indo-1 fluorescence from perfused rabbit hearts</title><author>Fralix, Teresa A. ; Heineman, Frederick W. ; Balaban, Robert S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4112-8498e7cd8c134aae226ff9edf39a5d7a89af1200146994253d4ed732d576ae83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>A23187</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological effects of radiation</topic><topic>Calcium</topic><topic>Cyanides - pharmacology</topic><topic>Cytochrome</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heart - drug effects</topic><topic>Heart - physiology</topic><topic>Hypoxia</topic><topic>Hypoxia - pathology</topic><topic>In Vitro Techniques</topic><topic>Indoles</topic><topic>Inner filter</topic><topic>Myoglobin</topic><topic>NADH</topic><topic>NADP</topic><topic>Non ionizing radiations. Hertzian waves. Biooptics</topic><topic>Optical spectroscopy</topic><topic>Perfusion</topic><topic>Rabbits</topic><topic>Spectrofluorometry</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Tissues, organs and organisms biophysics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fralix, Teresa A.</creatorcontrib><creatorcontrib>Heineman, Frederick W.</creatorcontrib><creatorcontrib>Balaban, Robert S.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fralix, Teresa A.</au><au>Heineman, Frederick W.</au><au>Balaban, Robert S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of tissue absorbance on NAD(P)H and Indo-1 fluorescence from perfused rabbit hearts</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1990-03-26</date><risdate>1990</risdate><volume>262</volume><issue>2</issue><spage>287</spage><epage>292</epage><pages>287-292</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>The effects of tissue optical absorbance on intracellular NAD(P)H and Indo-1 fluorescence emission have been evaluated in the perfused rabbit heart. These results demonstrate that the tissue optical absorbance significantly modifies the emission characteristics of these fluorophores. This tissue ‘inner filter’ effect, observed with both probes, changed as a function of tissue oxygenation and redox state in a wavelength-dependent manner. Pathlength calculations from these results indicate that this inner filter effect could occur with a mean pathlength of 310 μm due to the extremely high extinction coefficient of heart tissue. It is concluded that tissue optical absorbance significantly affects the fluorescent emission characteristics of both intrinsic and extrinsic probes in the intact heart, under a variety of conditions. Several potential methods of correcting for these tissue inner filter effects are discussed.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2335209</pmid><doi>10.1016/0014-5793(90)80212-2</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1990-03, Vol.262 (2), p.287-292 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | A23187 Animals Biological and medical sciences Biological effects of radiation Calcium Cyanides - pharmacology Cytochrome Fundamental and applied biological sciences. Psychology Heart - drug effects Heart - physiology Hypoxia Hypoxia - pathology In Vitro Techniques Indoles Inner filter Myoglobin NADH NADP Non ionizing radiations. Hertzian waves. Biooptics Optical spectroscopy Perfusion Rabbits Spectrofluorometry Spectrometry, Fluorescence - methods Tissues, organs and organisms biophysics |
| title | Effects of tissue absorbance on NAD(P)H and Indo-1 fluorescence from perfused rabbit hearts |
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