Hydrocortisone Reinforces the Blood–Brain Barrier Properties in a Serum Free Cell Culture System
The increasing number of newly developed drugs demands for functionalin vitromodels of the blood-brain barrier to determine their brain uptake. Cultured cerebral capillary endothelial cells are considered to be such a model, however in serum containing media they exhibit low electrical resistances a...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1998-03, Vol.244 (1), p.312-316 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Hoheisel, Dirk Nitz, Thorsten Franke, Helmut Wegener, Joachim Hakvoort, Ansgar Tilling, Thomas Galla, Hans-Joachim |
| description | The increasing number of newly developed drugs demands for functionalin vitromodels of the blood-brain barrier to determine their brain uptake. Cultured cerebral capillary endothelial cells are considered to be such a model, however in serum containing media they exhibit low electrical resistances and high permeabilities compared to thein vivosituation. Here we report the establishment of a serum-free cell culture model. Withdrawal of serum already caused a twofold increase of transendothelial resistance (TER), which in presence of serum is about 100-150 Ω·cm2. We tested several supplements and found that hydrocortisone is a potent stimulator for the formation of barrier properties. TERs up to 1000 Ω·cm2were measured in the presence of physiological relevant hydrocortisone concentrations. In correspondence to the TER increase hydrocortisone decreased cell monolayer permeability for sucrose down to 5·10−7cm/s, which is close to thein vivovalue of 1.2·10−7cm/s and by a factor of five lower compared to cultures without hydrocortisone and in presence of serum. |
| doi_str_mv | 10.1006/bbrc.1997.8051 |
| format | Article |
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Cultured cerebral capillary endothelial cells are considered to be such a model, however in serum containing media they exhibit low electrical resistances and high permeabilities compared to thein vivosituation. Here we report the establishment of a serum-free cell culture model. Withdrawal of serum already caused a twofold increase of transendothelial resistance (TER), which in presence of serum is about 100-150 Ω·cm2. We tested several supplements and found that hydrocortisone is a potent stimulator for the formation of barrier properties. TERs up to 1000 Ω·cm2were measured in the presence of physiological relevant hydrocortisone concentrations. In correspondence to the TER increase hydrocortisone decreased cell monolayer permeability for sucrose down to 5·10−7cm/s, which is close to thein vivovalue of 1.2·10−7cm/s and by a factor of five lower compared to cultures without hydrocortisone and in presence of serum.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1997.8051</identifier><identifier>PMID: 9514852</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Blood-Brain Barrier - drug effects ; Capillary Permeability - drug effects ; Capillary Resistance - drug effects ; Cell Culture Techniques - methods ; Cerebral Cortex - blood supply ; Cerebral Cortex - cytology ; Culture Media, Serum-Free ; Endothelium, Vascular - drug effects ; Epidermal Growth Factor - pharmacology ; Hydrocortisone - pharmacology ; Insulin - pharmacology ; Models, Biological ; Sucrose - metabolism ; Swine</subject><ispartof>Biochemical and biophysical research communications, 1998-03, Vol.244 (1), p.312-316</ispartof><rights>1998 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c320t-ae9bcddd8f2f114a78017afcf577ebdc6c49f655bd54c3acb0ec0b12c212b4613</citedby><cites>FETCH-LOGICAL-c320t-ae9bcddd8f2f114a78017afcf577ebdc6c49f655bd54c3acb0ec0b12c212b4613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1997.8051$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9514852$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hoheisel, Dirk</creatorcontrib><creatorcontrib>Nitz, Thorsten</creatorcontrib><creatorcontrib>Franke, Helmut</creatorcontrib><creatorcontrib>Wegener, Joachim</creatorcontrib><creatorcontrib>Hakvoort, Ansgar</creatorcontrib><creatorcontrib>Tilling, Thomas</creatorcontrib><creatorcontrib>Galla, Hans-Joachim</creatorcontrib><title>Hydrocortisone Reinforces the Blood–Brain Barrier Properties in a Serum Free Cell Culture System</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The increasing number of newly developed drugs demands for functionalin vitromodels of the blood-brain barrier to determine their brain uptake. Cultured cerebral capillary endothelial cells are considered to be such a model, however in serum containing media they exhibit low electrical resistances and high permeabilities compared to thein vivosituation. Here we report the establishment of a serum-free cell culture model. Withdrawal of serum already caused a twofold increase of transendothelial resistance (TER), which in presence of serum is about 100-150 Ω·cm2. We tested several supplements and found that hydrocortisone is a potent stimulator for the formation of barrier properties. TERs up to 1000 Ω·cm2were measured in the presence of physiological relevant hydrocortisone concentrations. In correspondence to the TER increase hydrocortisone decreased cell monolayer permeability for sucrose down to 5·10−7cm/s, which is close to thein vivovalue of 1.2·10−7cm/s and by a factor of five lower compared to cultures without hydrocortisone and in presence of serum.</description><subject>Animals</subject><subject>Blood-Brain Barrier - drug effects</subject><subject>Capillary Permeability - drug effects</subject><subject>Capillary Resistance - drug effects</subject><subject>Cell Culture Techniques - methods</subject><subject>Cerebral Cortex - blood supply</subject><subject>Cerebral Cortex - cytology</subject><subject>Culture Media, Serum-Free</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Hydrocortisone - pharmacology</subject><subject>Insulin - pharmacology</subject><subject>Models, Biological</subject><subject>Sucrose - metabolism</subject><subject>Swine</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE2LFDEQhsPiss6uXvcm5OStxyTTXzk6w34ICy6ugreQVKox0t0ZK93C3PY_-A_9JZtmBm-eCqqeeql6GLuWYi2FqD84R7CWWjfrVlTyjK2k0KJQUpSv2EpkolBafn_NLlP6KYSUZa0v2IWuZNlWasXc_cFThEhTSHFE_gXD2EUCTHz6gXzbx-j_Pv_Zkg0j31qigMQfKe4xb2Qody1_QpoHfkuIfId9z3dzP82E_OmQJhzesPPO9gnfnuoV-3Z783V3Xzx8vvu0-_hQwEaJqbCoHXjv2051-UzbtEI2toOuahp0HmoodVdXlfNVCRsLTiAIJxUoqVxZy80Ve3_M3VP8NWOazBAS5HvsiHFOptFNVSq5gOsjCBRTIuzMnsJg6WCkMItUs0g1i1SzSM0L707JsxvQ_8NPFvO8Pc4xv_c7GzIJAo6APhDCZHwM_4t-AcE4iLI</recordid><startdate>19980306</startdate><enddate>19980306</enddate><creator>Hoheisel, Dirk</creator><creator>Nitz, Thorsten</creator><creator>Franke, Helmut</creator><creator>Wegener, Joachim</creator><creator>Hakvoort, Ansgar</creator><creator>Tilling, Thomas</creator><creator>Galla, Hans-Joachim</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980306</creationdate><title>Hydrocortisone Reinforces the Blood–Brain Barrier Properties in a Serum Free Cell Culture System</title><author>Hoheisel, Dirk ; 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Cultured cerebral capillary endothelial cells are considered to be such a model, however in serum containing media they exhibit low electrical resistances and high permeabilities compared to thein vivosituation. Here we report the establishment of a serum-free cell culture model. Withdrawal of serum already caused a twofold increase of transendothelial resistance (TER), which in presence of serum is about 100-150 Ω·cm2. We tested several supplements and found that hydrocortisone is a potent stimulator for the formation of barrier properties. TERs up to 1000 Ω·cm2were measured in the presence of physiological relevant hydrocortisone concentrations. 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| ispartof | Biochemical and biophysical research communications, 1998-03, Vol.244 (1), p.312-316 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Blood-Brain Barrier - drug effects Capillary Permeability - drug effects Capillary Resistance - drug effects Cell Culture Techniques - methods Cerebral Cortex - blood supply Cerebral Cortex - cytology Culture Media, Serum-Free Endothelium, Vascular - drug effects Epidermal Growth Factor - pharmacology Hydrocortisone - pharmacology Insulin - pharmacology Models, Biological Sucrose - metabolism Swine |
| title | Hydrocortisone Reinforces the Blood–Brain Barrier Properties in a Serum Free Cell Culture System |
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