Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection
Chronic lung rejection (CLR) induces a fibroproliferative disorder leading to the occlusion of small airways. It has emerged as the major factor limiting the survival of lung transplant recipients. Predictive markers of CLR are lacking, and its diagnosis is generally ascertained when the fibrosis pr...
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| Veröffentlicht in: | Transplantation 1998-03, Vol.65 (5), p.752-755 |
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| creator | CHARPIN, J. M VALCKE, J KETTANEH, L EPARDEAU, B STERN, M ISRAËL-BIET, D |
| description | Chronic lung rejection (CLR) induces a fibroproliferative disorder leading to the occlusion of small airways. It has emerged as the major factor limiting the survival of lung transplant recipients. Predictive markers of CLR are lacking, and its diagnosis is generally ascertained when the fibrosis process is irreversible.
We have quantified the expression of transforming growth factor-beta (TGF-beta), a critical mediator of fibrogenesis, in alveolar cells from lung transplant recipients using a competitive reverse transcriptase polymerase chain reaction method.
We have shown that patients with CLR presented marked peaks of TGF-beta mRNA expression, in contrast with patients without CLR. These peaks preceded the diagnosis of CLR by several months in two of three patients who died within 2 years of diagnosis.
Our data suggest that TGF-beta expression in alveolar cells could serve as an early predictive and prognostic marker of chronic lung rejection. |
| doi_str_mv | 10.1097/00007890-199803150-00027 |
| format | Article |
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We have quantified the expression of transforming growth factor-beta (TGF-beta), a critical mediator of fibrogenesis, in alveolar cells from lung transplant recipients using a competitive reverse transcriptase polymerase chain reaction method.
We have shown that patients with CLR presented marked peaks of TGF-beta mRNA expression, in contrast with patients without CLR. These peaks preceded the diagnosis of CLR by several months in two of three patients who died within 2 years of diagnosis.
Our data suggest that TGF-beta expression in alveolar cells could serve as an early predictive and prognostic marker of chronic lung rejection.</description><identifier>ISSN: 0041-1337</identifier><identifier>EISSN: 1534-6080</identifier><identifier>DOI: 10.1097/00007890-199803150-00027</identifier><identifier>PMID: 9521216</identifier><identifier>CODEN: TRPLAU</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott</publisher><subject>Biological and medical sciences ; Bronchoalveolar Lavage Fluid - chemistry ; Cytomegalovirus Infections - complications ; Cytomegalovirus Infections - immunology ; Gene Expression ; Graft Rejection - diagnosis ; Humans ; Lung Transplantation - immunology ; Medical sciences ; Pulmonary Alveoli - immunology ; Pulmonary Alveoli - metabolism ; RNA, Messenger - metabolism ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the respiratory system ; Time Factors ; Transforming Growth Factor beta - genetics</subject><ispartof>Transplantation, 1998-03, Vol.65 (5), p.752-755</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2191483$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9521216$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHARPIN, J. M</creatorcontrib><creatorcontrib>VALCKE, J</creatorcontrib><creatorcontrib>KETTANEH, L</creatorcontrib><creatorcontrib>EPARDEAU, B</creatorcontrib><creatorcontrib>STERN, M</creatorcontrib><creatorcontrib>ISRAËL-BIET, D</creatorcontrib><title>Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection</title><title>Transplantation</title><addtitle>Transplantation</addtitle><description>Chronic lung rejection (CLR) induces a fibroproliferative disorder leading to the occlusion of small airways. It has emerged as the major factor limiting the survival of lung transplant recipients. Predictive markers of CLR are lacking, and its diagnosis is generally ascertained when the fibrosis process is irreversible.
We have quantified the expression of transforming growth factor-beta (TGF-beta), a critical mediator of fibrogenesis, in alveolar cells from lung transplant recipients using a competitive reverse transcriptase polymerase chain reaction method.
We have shown that patients with CLR presented marked peaks of TGF-beta mRNA expression, in contrast with patients without CLR. These peaks preceded the diagnosis of CLR by several months in two of three patients who died within 2 years of diagnosis.
Our data suggest that TGF-beta expression in alveolar cells could serve as an early predictive and prognostic marker of chronic lung rejection.</description><subject>Biological and medical sciences</subject><subject>Bronchoalveolar Lavage Fluid - chemistry</subject><subject>Cytomegalovirus Infections - complications</subject><subject>Cytomegalovirus Infections - immunology</subject><subject>Gene Expression</subject><subject>Graft Rejection - diagnosis</subject><subject>Humans</subject><subject>Lung Transplantation - immunology</subject><subject>Medical sciences</subject><subject>Pulmonary Alveoli - immunology</subject><subject>Pulmonary Alveoli - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the respiratory system</subject><subject>Time Factors</subject><subject>Transforming Growth Factor beta - genetics</subject><issn>0041-1337</issn><issn>1534-6080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkNFqFTEQhoMo9Vh9BCEX4t3WTLJJNpelWBWKiuj1YTZntk2bTdZkT6XXvpEP4jOZ1oO3DgMDM98_8P-McRAnIJx9I1rZwYkOnBuEAi26tpH2EduAVn1nxCAes40QPXSglH3KntV63RCtrD1iR05LkGA27OdnwpvK88TXgqlOucwhXfLLkn-sV3xCv-bS_f7F5y8fT3lIHOMt5YiFe4rxQRf3jX8QLxHTygv5sARKa-XYOnHCEu_4jOWGyr3AX5Wcgm_gNfk15PScPZkwVnpxmMfs2_nbr2fvu4tP7z6cnV50izRm7bT2IwlQk5EgwPlRGtCarJp6NWiUhqwAGCWSBDJCojV22E07AmlHpFEds9d__y4lf99TXbdzqPc-MFHe1611Vres-v-CYHohpYMGvjyA-3Gm3XYpofm82x7ibfdXhztWj3FqKflQ_2ESHPSDUn8A61eMbg</recordid><startdate>19980315</startdate><enddate>19980315</enddate><creator>CHARPIN, J. M</creator><creator>VALCKE, J</creator><creator>KETTANEH, L</creator><creator>EPARDEAU, B</creator><creator>STERN, M</creator><creator>ISRAËL-BIET, D</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19980315</creationdate><title>Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection</title><author>CHARPIN, J. M ; VALCKE, J ; KETTANEH, L ; EPARDEAU, B ; STERN, M ; ISRAËL-BIET, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p266t-55cbe013f621019cb26155e73f4385a26e7011b2ae21e602a7678dfde127baeb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological and medical sciences</topic><topic>Bronchoalveolar Lavage Fluid - chemistry</topic><topic>Cytomegalovirus Infections - complications</topic><topic>Cytomegalovirus Infections - immunology</topic><topic>Gene Expression</topic><topic>Graft Rejection - diagnosis</topic><topic>Humans</topic><topic>Lung Transplantation - immunology</topic><topic>Medical sciences</topic><topic>Pulmonary Alveoli - immunology</topic><topic>Pulmonary Alveoli - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the respiratory system</topic><topic>Time Factors</topic><topic>Transforming Growth Factor beta - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHARPIN, J. M</creatorcontrib><creatorcontrib>VALCKE, J</creatorcontrib><creatorcontrib>KETTANEH, L</creatorcontrib><creatorcontrib>EPARDEAU, B</creatorcontrib><creatorcontrib>STERN, M</creatorcontrib><creatorcontrib>ISRAËL-BIET, D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHARPIN, J. M</au><au>VALCKE, J</au><au>KETTANEH, L</au><au>EPARDEAU, B</au><au>STERN, M</au><au>ISRAËL-BIET, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection</atitle><jtitle>Transplantation</jtitle><addtitle>Transplantation</addtitle><date>1998-03-15</date><risdate>1998</risdate><volume>65</volume><issue>5</issue><spage>752</spage><epage>755</epage><pages>752-755</pages><issn>0041-1337</issn><eissn>1534-6080</eissn><coden>TRPLAU</coden><abstract>Chronic lung rejection (CLR) induces a fibroproliferative disorder leading to the occlusion of small airways. It has emerged as the major factor limiting the survival of lung transplant recipients. Predictive markers of CLR are lacking, and its diagnosis is generally ascertained when the fibrosis process is irreversible.
We have quantified the expression of transforming growth factor-beta (TGF-beta), a critical mediator of fibrogenesis, in alveolar cells from lung transplant recipients using a competitive reverse transcriptase polymerase chain reaction method.
We have shown that patients with CLR presented marked peaks of TGF-beta mRNA expression, in contrast with patients without CLR. These peaks preceded the diagnosis of CLR by several months in two of three patients who died within 2 years of diagnosis.
Our data suggest that TGF-beta expression in alveolar cells could serve as an early predictive and prognostic marker of chronic lung rejection.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>9521216</pmid><doi>10.1097/00007890-199803150-00027</doi><tpages>4</tpages></addata></record> |
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| ispartof | Transplantation, 1998-03, Vol.65 (5), p.752-755 |
| issn | 0041-1337 1534-6080 |
| language | eng |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Biological and medical sciences Bronchoalveolar Lavage Fluid - chemistry Cytomegalovirus Infections - complications Cytomegalovirus Infections - immunology Gene Expression Graft Rejection - diagnosis Humans Lung Transplantation - immunology Medical sciences Pulmonary Alveoli - immunology Pulmonary Alveoli - metabolism RNA, Messenger - metabolism Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the respiratory system Time Factors Transforming Growth Factor beta - genetics |
| title | Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection |
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