Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection

Chronic lung rejection (CLR) induces a fibroproliferative disorder leading to the occlusion of small airways. It has emerged as the major factor limiting the survival of lung transplant recipients. Predictive markers of CLR are lacking, and its diagnosis is generally ascertained when the fibrosis pr...

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Veröffentlicht in:Transplantation 1998-03, Vol.65 (5), p.752-755
Hauptverfasser: CHARPIN, J. M, VALCKE, J, KETTANEH, L, EPARDEAU, B, STERN, M, ISRAËL-BIET, D
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container_end_page 755
container_issue 5
container_start_page 752
container_title Transplantation
container_volume 65
creator CHARPIN, J. M
VALCKE, J
KETTANEH, L
EPARDEAU, B
STERN, M
ISRAËL-BIET, D
description Chronic lung rejection (CLR) induces a fibroproliferative disorder leading to the occlusion of small airways. It has emerged as the major factor limiting the survival of lung transplant recipients. Predictive markers of CLR are lacking, and its diagnosis is generally ascertained when the fibrosis process is irreversible. We have quantified the expression of transforming growth factor-beta (TGF-beta), a critical mediator of fibrogenesis, in alveolar cells from lung transplant recipients using a competitive reverse transcriptase polymerase chain reaction method. We have shown that patients with CLR presented marked peaks of TGF-beta mRNA expression, in contrast with patients without CLR. These peaks preceded the diagnosis of CLR by several months in two of three patients who died within 2 years of diagnosis. Our data suggest that TGF-beta expression in alveolar cells could serve as an early predictive and prognostic marker of chronic lung rejection.
doi_str_mv 10.1097/00007890-199803150-00027
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source MEDLINE; Journals@Ovid Complete
subjects Biological and medical sciences
Bronchoalveolar Lavage Fluid - chemistry
Cytomegalovirus Infections - complications
Cytomegalovirus Infections - immunology
Gene Expression
Graft Rejection - diagnosis
Humans
Lung Transplantation - immunology
Medical sciences
Pulmonary Alveoli - immunology
Pulmonary Alveoli - metabolism
RNA, Messenger - metabolism
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgery of the respiratory system
Time Factors
Transforming Growth Factor beta - genetics
title Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection
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