Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection

Chronic lung rejection (CLR) induces a fibroproliferative disorder leading to the occlusion of small airways. It has emerged as the major factor limiting the survival of lung transplant recipients. Predictive markers of CLR are lacking, and its diagnosis is generally ascertained when the fibrosis pr...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Transplantation 1998-03, Vol.65 (5), p.752-755
Hauptverfasser: CHARPIN, J. M, VALCKE, J, KETTANEH, L, EPARDEAU, B, STERN, M, ISRAËL-BIET, D
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 755
container_issue 5
container_start_page 752
container_title Transplantation
container_volume 65
creator CHARPIN, J. M
VALCKE, J
KETTANEH, L
EPARDEAU, B
STERN, M
ISRAËL-BIET, D
description Chronic lung rejection (CLR) induces a fibroproliferative disorder leading to the occlusion of small airways. It has emerged as the major factor limiting the survival of lung transplant recipients. Predictive markers of CLR are lacking, and its diagnosis is generally ascertained when the fibrosis process is irreversible. We have quantified the expression of transforming growth factor-beta (TGF-beta), a critical mediator of fibrogenesis, in alveolar cells from lung transplant recipients using a competitive reverse transcriptase polymerase chain reaction method. We have shown that patients with CLR presented marked peaks of TGF-beta mRNA expression, in contrast with patients without CLR. These peaks preceded the diagnosis of CLR by several months in two of three patients who died within 2 years of diagnosis. Our data suggest that TGF-beta expression in alveolar cells could serve as an early predictive and prognostic marker of chronic lung rejection.
doi_str_mv 10.1097/00007890-199803150-00027
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_79753374</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79753374</sourcerecordid><originalsourceid>FETCH-LOGICAL-p266t-55cbe013f621019cb26155e73f4385a26e7011b2ae21e602a7678dfde127baeb3</originalsourceid><addsrcrecordid>eNqFkNFqFTEQhoMo9Vh9BCEX4t3WTLJJNpelWBWKiuj1YTZntk2bTdZkT6XXvpEP4jOZ1oO3DgMDM98_8P-McRAnIJx9I1rZwYkOnBuEAi26tpH2EduAVn1nxCAes40QPXSglH3KntV63RCtrD1iR05LkGA27OdnwpvK88TXgqlOucwhXfLLkn-sV3xCv-bS_f7F5y8fT3lIHOMt5YiFe4rxQRf3jX8QLxHTygv5sARKa-XYOnHCEu_4jOWGyr3AX5Wcgm_gNfk15PScPZkwVnpxmMfs2_nbr2fvu4tP7z6cnV50izRm7bT2IwlQk5EgwPlRGtCarJp6NWiUhqwAGCWSBDJCojV22E07AmlHpFEds9d__y4lf99TXbdzqPc-MFHe1611Vres-v-CYHohpYMGvjyA-3Gm3XYpofm82x7ibfdXhztWj3FqKflQ_2ESHPSDUn8A61eMbg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16402291</pqid></control><display><type>article</type><title>Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><creator>CHARPIN, J. M ; VALCKE, J ; KETTANEH, L ; EPARDEAU, B ; STERN, M ; ISRAËL-BIET, D</creator><creatorcontrib>CHARPIN, J. M ; VALCKE, J ; KETTANEH, L ; EPARDEAU, B ; STERN, M ; ISRAËL-BIET, D</creatorcontrib><description>Chronic lung rejection (CLR) induces a fibroproliferative disorder leading to the occlusion of small airways. It has emerged as the major factor limiting the survival of lung transplant recipients. Predictive markers of CLR are lacking, and its diagnosis is generally ascertained when the fibrosis process is irreversible. We have quantified the expression of transforming growth factor-beta (TGF-beta), a critical mediator of fibrogenesis, in alveolar cells from lung transplant recipients using a competitive reverse transcriptase polymerase chain reaction method. We have shown that patients with CLR presented marked peaks of TGF-beta mRNA expression, in contrast with patients without CLR. These peaks preceded the diagnosis of CLR by several months in two of three patients who died within 2 years of diagnosis. Our data suggest that TGF-beta expression in alveolar cells could serve as an early predictive and prognostic marker of chronic lung rejection.</description><identifier>ISSN: 0041-1337</identifier><identifier>EISSN: 1534-6080</identifier><identifier>DOI: 10.1097/00007890-199803150-00027</identifier><identifier>PMID: 9521216</identifier><identifier>CODEN: TRPLAU</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott</publisher><subject>Biological and medical sciences ; Bronchoalveolar Lavage Fluid - chemistry ; Cytomegalovirus Infections - complications ; Cytomegalovirus Infections - immunology ; Gene Expression ; Graft Rejection - diagnosis ; Humans ; Lung Transplantation - immunology ; Medical sciences ; Pulmonary Alveoli - immunology ; Pulmonary Alveoli - metabolism ; RNA, Messenger - metabolism ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the respiratory system ; Time Factors ; Transforming Growth Factor beta - genetics</subject><ispartof>Transplantation, 1998-03, Vol.65 (5), p.752-755</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=2191483$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9521216$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHARPIN, J. M</creatorcontrib><creatorcontrib>VALCKE, J</creatorcontrib><creatorcontrib>KETTANEH, L</creatorcontrib><creatorcontrib>EPARDEAU, B</creatorcontrib><creatorcontrib>STERN, M</creatorcontrib><creatorcontrib>ISRAËL-BIET, D</creatorcontrib><title>Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection</title><title>Transplantation</title><addtitle>Transplantation</addtitle><description>Chronic lung rejection (CLR) induces a fibroproliferative disorder leading to the occlusion of small airways. It has emerged as the major factor limiting the survival of lung transplant recipients. Predictive markers of CLR are lacking, and its diagnosis is generally ascertained when the fibrosis process is irreversible. We have quantified the expression of transforming growth factor-beta (TGF-beta), a critical mediator of fibrogenesis, in alveolar cells from lung transplant recipients using a competitive reverse transcriptase polymerase chain reaction method. We have shown that patients with CLR presented marked peaks of TGF-beta mRNA expression, in contrast with patients without CLR. These peaks preceded the diagnosis of CLR by several months in two of three patients who died within 2 years of diagnosis. Our data suggest that TGF-beta expression in alveolar cells could serve as an early predictive and prognostic marker of chronic lung rejection.</description><subject>Biological and medical sciences</subject><subject>Bronchoalveolar Lavage Fluid - chemistry</subject><subject>Cytomegalovirus Infections - complications</subject><subject>Cytomegalovirus Infections - immunology</subject><subject>Gene Expression</subject><subject>Graft Rejection - diagnosis</subject><subject>Humans</subject><subject>Lung Transplantation - immunology</subject><subject>Medical sciences</subject><subject>Pulmonary Alveoli - immunology</subject><subject>Pulmonary Alveoli - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the respiratory system</subject><subject>Time Factors</subject><subject>Transforming Growth Factor beta - genetics</subject><issn>0041-1337</issn><issn>1534-6080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkNFqFTEQhoMo9Vh9BCEX4t3WTLJJNpelWBWKiuj1YTZntk2bTdZkT6XXvpEP4jOZ1oO3DgMDM98_8P-McRAnIJx9I1rZwYkOnBuEAi26tpH2EduAVn1nxCAes40QPXSglH3KntV63RCtrD1iR05LkGA27OdnwpvK88TXgqlOucwhXfLLkn-sV3xCv-bS_f7F5y8fT3lIHOMt5YiFe4rxQRf3jX8QLxHTygv5sARKa-XYOnHCEu_4jOWGyr3AX5Wcgm_gNfk15PScPZkwVnpxmMfs2_nbr2fvu4tP7z6cnV50izRm7bT2IwlQk5EgwPlRGtCarJp6NWiUhqwAGCWSBDJCojV22E07AmlHpFEds9d__y4lf99TXbdzqPc-MFHe1611Vres-v-CYHohpYMGvjyA-3Gm3XYpofm82x7ibfdXhztWj3FqKflQ_2ESHPSDUn8A61eMbg</recordid><startdate>19980315</startdate><enddate>19980315</enddate><creator>CHARPIN, J. M</creator><creator>VALCKE, J</creator><creator>KETTANEH, L</creator><creator>EPARDEAU, B</creator><creator>STERN, M</creator><creator>ISRAËL-BIET, D</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19980315</creationdate><title>Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection</title><author>CHARPIN, J. M ; VALCKE, J ; KETTANEH, L ; EPARDEAU, B ; STERN, M ; ISRAËL-BIET, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p266t-55cbe013f621019cb26155e73f4385a26e7011b2ae21e602a7678dfde127baeb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological and medical sciences</topic><topic>Bronchoalveolar Lavage Fluid - chemistry</topic><topic>Cytomegalovirus Infections - complications</topic><topic>Cytomegalovirus Infections - immunology</topic><topic>Gene Expression</topic><topic>Graft Rejection - diagnosis</topic><topic>Humans</topic><topic>Lung Transplantation - immunology</topic><topic>Medical sciences</topic><topic>Pulmonary Alveoli - immunology</topic><topic>Pulmonary Alveoli - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the respiratory system</topic><topic>Time Factors</topic><topic>Transforming Growth Factor beta - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHARPIN, J. M</creatorcontrib><creatorcontrib>VALCKE, J</creatorcontrib><creatorcontrib>KETTANEH, L</creatorcontrib><creatorcontrib>EPARDEAU, B</creatorcontrib><creatorcontrib>STERN, M</creatorcontrib><creatorcontrib>ISRAËL-BIET, D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHARPIN, J. M</au><au>VALCKE, J</au><au>KETTANEH, L</au><au>EPARDEAU, B</au><au>STERN, M</au><au>ISRAËL-BIET, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection</atitle><jtitle>Transplantation</jtitle><addtitle>Transplantation</addtitle><date>1998-03-15</date><risdate>1998</risdate><volume>65</volume><issue>5</issue><spage>752</spage><epage>755</epage><pages>752-755</pages><issn>0041-1337</issn><eissn>1534-6080</eissn><coden>TRPLAU</coden><abstract>Chronic lung rejection (CLR) induces a fibroproliferative disorder leading to the occlusion of small airways. It has emerged as the major factor limiting the survival of lung transplant recipients. Predictive markers of CLR are lacking, and its diagnosis is generally ascertained when the fibrosis process is irreversible. We have quantified the expression of transforming growth factor-beta (TGF-beta), a critical mediator of fibrogenesis, in alveolar cells from lung transplant recipients using a competitive reverse transcriptase polymerase chain reaction method. We have shown that patients with CLR presented marked peaks of TGF-beta mRNA expression, in contrast with patients without CLR. These peaks preceded the diagnosis of CLR by several months in two of three patients who died within 2 years of diagnosis. Our data suggest that TGF-beta expression in alveolar cells could serve as an early predictive and prognostic marker of chronic lung rejection.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>9521216</pmid><doi>10.1097/00007890-199803150-00027</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0041-1337
ispartof Transplantation, 1998-03, Vol.65 (5), p.752-755
issn 0041-1337
1534-6080
language eng
recordid cdi_proquest_miscellaneous_79753374
source MEDLINE; Journals@Ovid Complete
subjects Biological and medical sciences
Bronchoalveolar Lavage Fluid - chemistry
Cytomegalovirus Infections - complications
Cytomegalovirus Infections - immunology
Gene Expression
Graft Rejection - diagnosis
Humans
Lung Transplantation - immunology
Medical sciences
Pulmonary Alveoli - immunology
Pulmonary Alveoli - metabolism
RNA, Messenger - metabolism
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgery of the respiratory system
Time Factors
Transforming Growth Factor beta - genetics
title Peaks of transforming growth factor-β mRNA in alveolar cells of lung transplant recipients as an early marker of chronic rejection
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-05T09%3A47%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Peaks%20of%20transforming%20growth%20factor-%CE%B2%20mRNA%20in%20alveolar%20cells%20of%20lung%20transplant%20recipients%20as%20an%20early%20marker%20of%20chronic%20rejection&rft.jtitle=Transplantation&rft.au=CHARPIN,%20J.%20M&rft.date=1998-03-15&rft.volume=65&rft.issue=5&rft.spage=752&rft.epage=755&rft.pages=752-755&rft.issn=0041-1337&rft.eissn=1534-6080&rft.coden=TRPLAU&rft_id=info:doi/10.1097/00007890-199803150-00027&rft_dat=%3Cproquest_pubme%3E79753374%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16402291&rft_id=info:pmid/9521216&rfr_iscdi=true