Allatinhibin, a neurohormonal inhibitor of juvenile hormone biosynthesis in Manduca sexta
The corpora allata (CA) of Manduca sexta larvae become incapable of synthesizing juvenile hormone (JH) early in the wandering stage of the last larval stadium. They then switch to the synthesis and release of JH acids. This change in CA activity is induced by an inhibitory factor—allatinhibin (AI)—f...
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| Veröffentlicht in: | General and comparative endocrinology 1990-04, Vol.78 (1), p.123-136 |
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| creator | Bhaskaran, Govindan Dahm, Karl H. Barrera, Punnee Pacheco, Jorge L. Peck, Kenneth E. Muszynska-Pytel, Malgorzata |
| description | The corpora allata (CA) of
Manduca sexta larvae become incapable of synthesizing juvenile hormone (JH) early in the wandering stage of the last larval stadium. They then switch to the synthesis and release of JH acids. This change in CA activity is induced by an inhibitory factor—allatinhibin (AI)—from the brain. AI activity is present in the fifth (Vth) instar hemolymph from about Day 4 (day of wandering) until Day 7 (early prepupa). CA of early fifth instar larvae (uninhibited) incubated
in vitro with brain-corpora cardiaca-corpora allata (Br-CC-CA) complexes or brain alone from wandering larvae are inhibited as demonstrated by bioassay. On the basis of these observations, an
in vitro-in vivo assay for AI was developed. Br-CC-CA or Br alone were first incubated in tissue culture medium overnight. Day 0 (0d) Vth instar CA incubated for 16 hr in such medium will lose the ability to induce a larval molt in allatectomized 0d IVth instar larvae if the medium contained AI activity. The highest AI activity was exhibited by the medium obtained from incubations of brain from wandering larvae whereas the medium from incubation of 0d Vth and 0d pupal brains showed no AI activity. Dose-response data show that AI is active at 0.03 brain equivalents/200 μl medium. CA must be exposed to AI for 12–16 hr for manifestation of inhibition. AI causes a stable inhibition of CA. AI is heat-labile, protease sensitive, has a molecular size between 1.0 and 3.5 kDa, and is clearly distinct from the allatostatins described by others. |
| doi_str_mv | 10.1016/0016-6480(90)90053-O |
| format | Article |
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Manduca sexta larvae become incapable of synthesizing juvenile hormone (JH) early in the wandering stage of the last larval stadium. They then switch to the synthesis and release of JH acids. This change in CA activity is induced by an inhibitory factor—allatinhibin (AI)—from the brain. AI activity is present in the fifth (Vth) instar hemolymph from about Day 4 (day of wandering) until Day 7 (early prepupa). CA of early fifth instar larvae (uninhibited) incubated
in vitro with brain-corpora cardiaca-corpora allata (Br-CC-CA) complexes or brain alone from wandering larvae are inhibited as demonstrated by bioassay. On the basis of these observations, an
in vitro-in vivo assay for AI was developed. Br-CC-CA or Br alone were first incubated in tissue culture medium overnight. Day 0 (0d) Vth instar CA incubated for 16 hr in such medium will lose the ability to induce a larval molt in allatectomized 0d IVth instar larvae if the medium contained AI activity. The highest AI activity was exhibited by the medium obtained from incubations of brain from wandering larvae whereas the medium from incubation of 0d Vth and 0d pupal brains showed no AI activity. Dose-response data show that AI is active at 0.03 brain equivalents/200 μl medium. CA must be exposed to AI for 12–16 hr for manifestation of inhibition. AI causes a stable inhibition of CA. AI is heat-labile, protease sensitive, has a molecular size between 1.0 and 3.5 kDa, and is clearly distinct from the allatostatins described by others.</description><identifier>ISSN: 0016-6480</identifier><identifier>EISSN: 1095-6840</identifier><identifier>DOI: 10.1016/0016-6480(90)90053-O</identifier><identifier>PMID: 1970545</identifier><identifier>CODEN: GCENA5</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Biochemistry. Physiology. Immunology ; Biological and medical sciences ; BIOSINTESIS ; BIOSYNTHESE ; BIOSYNTHESIS ; Brain - metabolism ; Corpora Allata - drug effects ; Corpora Allata - metabolism ; Fundamental and applied biological sciences. Psychology ; HORMONAS ; HORMONAS JUVENILES ; HORMONE ; HORMONE JUVENILE ; HORMONES ; Insect Hormones - metabolism ; Insect Hormones - pharmacology ; Insecta ; Invertebrates ; JUVENILE HORMONES ; Juvenile Hormones - biosynthesis ; Larva - drug effects ; Larva - metabolism ; LARVAE ; LARVAS ; LARVE ; Lepidoptera ; Lepidoptera - drug effects ; Lepidoptera - metabolism ; MANDUCA ; Manduca sexta ; NERVOUS SYSTEM ; NEUROHORMONAL CONTROL ; Neurotransmitter Agents - metabolism ; Neurotransmitter Agents - pharmacology ; Physiology. Development ; SISTEMA NERVIOSO ; Sphingidae ; SYSTEME NERVEUX</subject><ispartof>General and comparative endocrinology, 1990-04, Vol.78 (1), p.123-136</ispartof><rights>1990</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c553t-f4be8c4768469ee9c67d04ad5fafebe1fd9a19bd46afc8a319a59328fd47c063</citedby><cites>FETCH-LOGICAL-c553t-f4be8c4768469ee9c67d04ad5fafebe1fd9a19bd46afc8a319a59328fd47c063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0016-6480(90)90053-O$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6859438$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1970545$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bhaskaran, Govindan</creatorcontrib><creatorcontrib>Dahm, Karl H.</creatorcontrib><creatorcontrib>Barrera, Punnee</creatorcontrib><creatorcontrib>Pacheco, Jorge L.</creatorcontrib><creatorcontrib>Peck, Kenneth E.</creatorcontrib><creatorcontrib>Muszynska-Pytel, Malgorzata</creatorcontrib><title>Allatinhibin, a neurohormonal inhibitor of juvenile hormone biosynthesis in Manduca sexta</title><title>General and comparative endocrinology</title><addtitle>Gen Comp Endocrinol</addtitle><description>The corpora allata (CA) of
Manduca sexta larvae become incapable of synthesizing juvenile hormone (JH) early in the wandering stage of the last larval stadium. They then switch to the synthesis and release of JH acids. This change in CA activity is induced by an inhibitory factor—allatinhibin (AI)—from the brain. AI activity is present in the fifth (Vth) instar hemolymph from about Day 4 (day of wandering) until Day 7 (early prepupa). CA of early fifth instar larvae (uninhibited) incubated
in vitro with brain-corpora cardiaca-corpora allata (Br-CC-CA) complexes or brain alone from wandering larvae are inhibited as demonstrated by bioassay. On the basis of these observations, an
in vitro-in vivo assay for AI was developed. Br-CC-CA or Br alone were first incubated in tissue culture medium overnight. Day 0 (0d) Vth instar CA incubated for 16 hr in such medium will lose the ability to induce a larval molt in allatectomized 0d IVth instar larvae if the medium contained AI activity. The highest AI activity was exhibited by the medium obtained from incubations of brain from wandering larvae whereas the medium from incubation of 0d Vth and 0d pupal brains showed no AI activity. Dose-response data show that AI is active at 0.03 brain equivalents/200 μl medium. CA must be exposed to AI for 12–16 hr for manifestation of inhibition. AI causes a stable inhibition of CA. AI is heat-labile, protease sensitive, has a molecular size between 1.0 and 3.5 kDa, and is clearly distinct from the allatostatins described by others.</description><subject>Animals</subject><subject>Biochemistry. Physiology. Immunology</subject><subject>Biological and medical sciences</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>BIOSYNTHESIS</subject><subject>Brain - metabolism</subject><subject>Corpora Allata - drug effects</subject><subject>Corpora Allata - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HORMONAS</subject><subject>HORMONAS JUVENILES</subject><subject>HORMONE</subject><subject>HORMONE JUVENILE</subject><subject>HORMONES</subject><subject>Insect Hormones - metabolism</subject><subject>Insect Hormones - pharmacology</subject><subject>Insecta</subject><subject>Invertebrates</subject><subject>JUVENILE HORMONES</subject><subject>Juvenile Hormones - biosynthesis</subject><subject>Larva - drug effects</subject><subject>Larva - metabolism</subject><subject>LARVAE</subject><subject>LARVAS</subject><subject>LARVE</subject><subject>Lepidoptera</subject><subject>Lepidoptera - drug effects</subject><subject>Lepidoptera - metabolism</subject><subject>MANDUCA</subject><subject>Manduca sexta</subject><subject>NERVOUS SYSTEM</subject><subject>NEUROHORMONAL CONTROL</subject><subject>Neurotransmitter Agents - metabolism</subject><subject>Neurotransmitter Agents - pharmacology</subject><subject>Physiology. Development</subject><subject>SISTEMA NERVIOSO</subject><subject>Sphingidae</subject><subject>SYSTEME NERVEUX</subject><issn>0016-6480</issn><issn>1095-6840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQhoMo67j6B0ShDyIKtlamk3TnIiyLX7AyB9eDp1CdrjhZepI16V7cf2-aHtabQpE6vE8VlYexZxzecuDqHZSnVqKDVxpeawDZ1Lt7bMNBy1p1Au6zzR3ykD3K-QoWSPETdsJ1C1LIDftxNo44-bD3vQ9vKqwCzSnuYzrEgGO1BlNMVXTV1XxDwY9UrTFVvY_5Nkx7yj4XtPqKYZgtVpl-T_iYPXA4Znpy7Kfs8uOHy_PP9cXu05fzs4vaStlMtRM9dVa05WKlibRV7QACB-nQUU_cDRq57geh0NkOG65R6mbbuUG0FlRzyl6ua69T_DVTnszBZ0vlV4HinE2rWwkNwH9BLtUWtmoBxQraFHNO5Mx18gdMt4aDWcybRatZtBq9VJFqdmXs-XH_3B9o-Du0qi75i2OO2eLoEgbr8x2mOqlF0xXs6Yo5jAZ_poJ8_6ahaWTLS_h-DakIvfGUTLaegqXBJ7KTGaL_95F_ALOMqPk</recordid><startdate>19900401</startdate><enddate>19900401</enddate><creator>Bhaskaran, Govindan</creator><creator>Dahm, Karl H.</creator><creator>Barrera, Punnee</creator><creator>Pacheco, Jorge L.</creator><creator>Peck, Kenneth E.</creator><creator>Muszynska-Pytel, Malgorzata</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>19900401</creationdate><title>Allatinhibin, a neurohormonal inhibitor of juvenile hormone biosynthesis in Manduca sexta</title><author>Bhaskaran, Govindan ; Dahm, Karl H. ; Barrera, Punnee ; Pacheco, Jorge L. ; Peck, Kenneth E. ; Muszynska-Pytel, Malgorzata</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c553t-f4be8c4768469ee9c67d04ad5fafebe1fd9a19bd46afc8a319a59328fd47c063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Biochemistry. Physiology. Immunology</topic><topic>Biological and medical sciences</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>BIOSYNTHESIS</topic><topic>Brain - metabolism</topic><topic>Corpora Allata - drug effects</topic><topic>Corpora Allata - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HORMONAS</topic><topic>HORMONAS JUVENILES</topic><topic>HORMONE</topic><topic>HORMONE JUVENILE</topic><topic>HORMONES</topic><topic>Insect Hormones - metabolism</topic><topic>Insect Hormones - pharmacology</topic><topic>Insecta</topic><topic>Invertebrates</topic><topic>JUVENILE HORMONES</topic><topic>Juvenile Hormones - biosynthesis</topic><topic>Larva - drug effects</topic><topic>Larva - metabolism</topic><topic>LARVAE</topic><topic>LARVAS</topic><topic>LARVE</topic><topic>Lepidoptera</topic><topic>Lepidoptera - drug effects</topic><topic>Lepidoptera - metabolism</topic><topic>MANDUCA</topic><topic>Manduca sexta</topic><topic>NERVOUS SYSTEM</topic><topic>NEUROHORMONAL CONTROL</topic><topic>Neurotransmitter Agents - metabolism</topic><topic>Neurotransmitter Agents - pharmacology</topic><topic>Physiology. Development</topic><topic>SISTEMA NERVIOSO</topic><topic>Sphingidae</topic><topic>SYSTEME NERVEUX</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bhaskaran, Govindan</creatorcontrib><creatorcontrib>Dahm, Karl H.</creatorcontrib><creatorcontrib>Barrera, Punnee</creatorcontrib><creatorcontrib>Pacheco, Jorge L.</creatorcontrib><creatorcontrib>Peck, Kenneth E.</creatorcontrib><creatorcontrib>Muszynska-Pytel, Malgorzata</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>General and comparative endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bhaskaran, Govindan</au><au>Dahm, Karl H.</au><au>Barrera, Punnee</au><au>Pacheco, Jorge L.</au><au>Peck, Kenneth E.</au><au>Muszynska-Pytel, Malgorzata</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Allatinhibin, a neurohormonal inhibitor of juvenile hormone biosynthesis in Manduca sexta</atitle><jtitle>General and comparative endocrinology</jtitle><addtitle>Gen Comp Endocrinol</addtitle><date>1990-04-01</date><risdate>1990</risdate><volume>78</volume><issue>1</issue><spage>123</spage><epage>136</epage><pages>123-136</pages><issn>0016-6480</issn><eissn>1095-6840</eissn><coden>GCENA5</coden><abstract>The corpora allata (CA) of
Manduca sexta larvae become incapable of synthesizing juvenile hormone (JH) early in the wandering stage of the last larval stadium. They then switch to the synthesis and release of JH acids. This change in CA activity is induced by an inhibitory factor—allatinhibin (AI)—from the brain. AI activity is present in the fifth (Vth) instar hemolymph from about Day 4 (day of wandering) until Day 7 (early prepupa). CA of early fifth instar larvae (uninhibited) incubated
in vitro with brain-corpora cardiaca-corpora allata (Br-CC-CA) complexes or brain alone from wandering larvae are inhibited as demonstrated by bioassay. On the basis of these observations, an
in vitro-in vivo assay for AI was developed. Br-CC-CA or Br alone were first incubated in tissue culture medium overnight. Day 0 (0d) Vth instar CA incubated for 16 hr in such medium will lose the ability to induce a larval molt in allatectomized 0d IVth instar larvae if the medium contained AI activity. The highest AI activity was exhibited by the medium obtained from incubations of brain from wandering larvae whereas the medium from incubation of 0d Vth and 0d pupal brains showed no AI activity. Dose-response data show that AI is active at 0.03 brain equivalents/200 μl medium. CA must be exposed to AI for 12–16 hr for manifestation of inhibition. AI causes a stable inhibition of CA. AI is heat-labile, protease sensitive, has a molecular size between 1.0 and 3.5 kDa, and is clearly distinct from the allatostatins described by others.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1970545</pmid><doi>10.1016/0016-6480(90)90053-O</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0016-6480 |
| ispartof | General and comparative endocrinology, 1990-04, Vol.78 (1), p.123-136 |
| issn | 0016-6480 1095-6840 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Biochemistry. Physiology. Immunology Biological and medical sciences BIOSINTESIS BIOSYNTHESE BIOSYNTHESIS Brain - metabolism Corpora Allata - drug effects Corpora Allata - metabolism Fundamental and applied biological sciences. Psychology HORMONAS HORMONAS JUVENILES HORMONE HORMONE JUVENILE HORMONES Insect Hormones - metabolism Insect Hormones - pharmacology Insecta Invertebrates JUVENILE HORMONES Juvenile Hormones - biosynthesis Larva - drug effects Larva - metabolism LARVAE LARVAS LARVE Lepidoptera Lepidoptera - drug effects Lepidoptera - metabolism MANDUCA Manduca sexta NERVOUS SYSTEM NEUROHORMONAL CONTROL Neurotransmitter Agents - metabolism Neurotransmitter Agents - pharmacology Physiology. Development SISTEMA NERVIOSO Sphingidae SYSTEME NERVEUX |
| title | Allatinhibin, a neurohormonal inhibitor of juvenile hormone biosynthesis in Manduca sexta |
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