Lanthanum probing of cell membrane permeability in the rat heart: Pathological versus artefactual alterations
The influence of fixation on membrane permeability has been examined in the isolated rat heart using lanthanum as a permeability probe. Normal and ischaemic hearts were probed at various stages during a conventional fixation programme with either ionic or colloidal lanthanum and compared with lantha...
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| Veröffentlicht in: | Journal of electron microscopy technique 1990-04, Vol.14 (4), p.357-366 |
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| container_title | Journal of electron microscopy technique |
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| creator | Harper, I. S. Williams, K. Lochner, A. |
| description | The influence of fixation on membrane permeability has been examined in the isolated rat heart using lanthanum as a permeability probe. Normal and ischaemic hearts were probed at various stages during a conventional fixation programme with either ionic or colloidal lanthanum and compared with lanthanum saline administered prior to fixation. Fixation of the myocardium coincident with or followed by lanthanum probing resulted in an influx of the probe into most myocytes in normal tissue. Alterations in permeability after ischaemic episodes could not be distinguished from this artefact. However, lanthanum saline prior to fixation showed exclusion of the probe from normal tissue, while the increased permeability demonstrated after ischaemia was associated with declining myocardial performance during subsequent reperfusion. These results illustrate the need for caution in the application and evaluation of methods determining permeability in fixed tissue. Probes of differing size and charge permeated fixed tissue to varying degrees thereby implicating the formation of specific lesions during chemical fixation. |
| doi_str_mv | 10.1002/jemt.1060140411 |
| format | Article |
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S. ; Williams, K. ; Lochner, A.</creator><creatorcontrib>Harper, I. S. ; Williams, K. ; Lochner, A.</creatorcontrib><description>The influence of fixation on membrane permeability has been examined in the isolated rat heart using lanthanum as a permeability probe. Normal and ischaemic hearts were probed at various stages during a conventional fixation programme with either ionic or colloidal lanthanum and compared with lanthanum saline administered prior to fixation. Fixation of the myocardium coincident with or followed by lanthanum probing resulted in an influx of the probe into most myocytes in normal tissue. Alterations in permeability after ischaemic episodes could not be distinguished from this artefact. However, lanthanum saline prior to fixation showed exclusion of the probe from normal tissue, while the increased permeability demonstrated after ischaemia was associated with declining myocardial performance during subsequent reperfusion. These results illustrate the need for caution in the application and evaluation of methods determining permeability in fixed tissue. Probes of differing size and charge permeated fixed tissue to varying degrees thereby implicating the formation of specific lesions during chemical fixation.</description><identifier>ISSN: 0741-0581</identifier><identifier>EISSN: 1553-0817</identifier><identifier>DOI: 10.1002/jemt.1060140411</identifier><identifier>PMID: 2332812</identifier><identifier>CODEN: JEMTEC</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; Cell Membrane Permeability - drug effects ; Coronary Vessels - ultrastructure ; Diverse techniques ; Fixation ; Fixatives ; Fundamental and applied biological sciences. Psychology ; Ischaemia ; Ischemia - pathology ; Lanthanum - pharmacokinetics ; Lanthanum - pharmacology ; Male ; Microscopy, Electron - methods ; Molecular and cellular biology ; Myocardium ; Myocardium - metabolism ; Myocardium - ultrastructure ; Rats ; Rats, Inbred Strains ; Sarcolemmal permeability ; Tracers</subject><ispartof>Journal of electron microscopy technique, 1990-04, Vol.14 (4), p.357-366</ispartof><rights>Copyright © 1990 Wiley‐Liss, Inc.</rights><rights>1991 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4121-3dd2cdf621d32665e7d194aa14f546c686a94a8a48b55beaaeb09b94d61e8ea33</citedby><cites>FETCH-LOGICAL-c4121-3dd2cdf621d32665e7d194aa14f546c686a94a8a48b55beaaeb09b94d61e8ea33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjemt.1060140411$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjemt.1060140411$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19278358$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2332812$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harper, I. S.</creatorcontrib><creatorcontrib>Williams, K.</creatorcontrib><creatorcontrib>Lochner, A.</creatorcontrib><title>Lanthanum probing of cell membrane permeability in the rat heart: Pathological versus artefactual alterations</title><title>Journal of electron microscopy technique</title><addtitle>J. Elec. Microsc. Tech</addtitle><description>The influence of fixation on membrane permeability has been examined in the isolated rat heart using lanthanum as a permeability probe. Normal and ischaemic hearts were probed at various stages during a conventional fixation programme with either ionic or colloidal lanthanum and compared with lanthanum saline administered prior to fixation. Fixation of the myocardium coincident with or followed by lanthanum probing resulted in an influx of the probe into most myocytes in normal tissue. Alterations in permeability after ischaemic episodes could not be distinguished from this artefact. However, lanthanum saline prior to fixation showed exclusion of the probe from normal tissue, while the increased permeability demonstrated after ischaemia was associated with declining myocardial performance during subsequent reperfusion. These results illustrate the need for caution in the application and evaluation of methods determining permeability in fixed tissue. Probes of differing size and charge permeated fixed tissue to varying degrees thereby implicating the formation of specific lesions during chemical fixation.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Coronary Vessels - ultrastructure</subject><subject>Diverse techniques</subject><subject>Fixation</subject><subject>Fixatives</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ischaemia</subject><subject>Ischemia - pathology</subject><subject>Lanthanum - pharmacokinetics</subject><subject>Lanthanum - pharmacology</subject><subject>Male</subject><subject>Microscopy, Electron - methods</subject><subject>Molecular and cellular biology</subject><subject>Myocardium</subject><subject>Myocardium - metabolism</subject><subject>Myocardium - ultrastructure</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Sarcolemmal permeability</subject><subject>Tracers</subject><issn>0741-0581</issn><issn>1553-0817</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAUhS0EKkNhzQrJG9iF-u0EVqUqBTRAJQZ1ad0kNx2XPAbbaZl_j0czasWKlR_n3GOfj5CXnL3ljImTGxxS3hnGFVOcPyILrrUsWMntY7JgVvGC6ZI_Jc9ivGFMWW3FETkSUoqSiwUZljCmNYzzQDdhqv14TaeONtj3dMChDjAi3WAYEGrf-7SlfqRpjTRAomuEkN7RS0jrqZ-ufQM9vcUQ50izgB00ac5X0CfMdj-N8Tl50kEf8cVhPSY_P56vzj4Vy-8Xn89Ol0WjuOCFbFvRtJ0RvJXCGI225ZUC4KrTyjSmNJCPJaiy1rpGAKxZVVeqNRxLBCmPyZt9bu70e8aY3ODjrlSuM83R2coqY-XOeLI3NmGKMWDnNsEPELaOM7cD7HaA3QPgPPHqED3XA7b3_gPRrL8-6BAzkC4TbHx8iK2ELaUus-_93nfne9z-71n35fzr6p9fFPtpHxP-uZ-G8MvlXla7q28X7lJcffixlCvH5V-FJ6ZV</recordid><startdate>199004</startdate><enddate>199004</enddate><creator>Harper, I. S.</creator><creator>Williams, K.</creator><creator>Lochner, A.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199004</creationdate><title>Lanthanum probing of cell membrane permeability in the rat heart: Pathological versus artefactual alterations</title><author>Harper, I. S. ; Williams, K. ; Lochner, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4121-3dd2cdf621d32665e7d194aa14f546c686a94a8a48b55beaaeb09b94d61e8ea33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Coronary Vessels - ultrastructure</topic><topic>Diverse techniques</topic><topic>Fixation</topic><topic>Fixatives</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ischaemia</topic><topic>Ischemia - pathology</topic><topic>Lanthanum - pharmacokinetics</topic><topic>Lanthanum - pharmacology</topic><topic>Male</topic><topic>Microscopy, Electron - methods</topic><topic>Molecular and cellular biology</topic><topic>Myocardium</topic><topic>Myocardium - metabolism</topic><topic>Myocardium - ultrastructure</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Sarcolemmal permeability</topic><topic>Tracers</topic><toplevel>online_resources</toplevel><creatorcontrib>Harper, I. S.</creatorcontrib><creatorcontrib>Williams, K.</creatorcontrib><creatorcontrib>Lochner, A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of electron microscopy technique</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harper, I. S.</au><au>Williams, K.</au><au>Lochner, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lanthanum probing of cell membrane permeability in the rat heart: Pathological versus artefactual alterations</atitle><jtitle>Journal of electron microscopy technique</jtitle><addtitle>J. Elec. Microsc. Tech</addtitle><date>1990-04</date><risdate>1990</risdate><volume>14</volume><issue>4</issue><spage>357</spage><epage>366</epage><pages>357-366</pages><issn>0741-0581</issn><eissn>1553-0817</eissn><coden>JEMTEC</coden><abstract>The influence of fixation on membrane permeability has been examined in the isolated rat heart using lanthanum as a permeability probe. Normal and ischaemic hearts were probed at various stages during a conventional fixation programme with either ionic or colloidal lanthanum and compared with lanthanum saline administered prior to fixation. Fixation of the myocardium coincident with or followed by lanthanum probing resulted in an influx of the probe into most myocytes in normal tissue. Alterations in permeability after ischaemic episodes could not be distinguished from this artefact. However, lanthanum saline prior to fixation showed exclusion of the probe from normal tissue, while the increased permeability demonstrated after ischaemia was associated with declining myocardial performance during subsequent reperfusion. These results illustrate the need for caution in the application and evaluation of methods determining permeability in fixed tissue. Probes of differing size and charge permeated fixed tissue to varying degrees thereby implicating the formation of specific lesions during chemical fixation.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2332812</pmid><doi>10.1002/jemt.1060140411</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Cell Membrane Permeability - drug effects Coronary Vessels - ultrastructure Diverse techniques Fixation Fixatives Fundamental and applied biological sciences. Psychology Ischaemia Ischemia - pathology Lanthanum - pharmacokinetics Lanthanum - pharmacology Male Microscopy, Electron - methods Molecular and cellular biology Myocardium Myocardium - metabolism Myocardium - ultrastructure Rats Rats, Inbred Strains Sarcolemmal permeability Tracers |
| title | Lanthanum probing of cell membrane permeability in the rat heart: Pathological versus artefactual alterations |
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