Diazepam binding inhibitor (DBI) processing : immunohistochemical studies in the rat brain

Diazepam Binding Inhibitor (DBI) is an endogenous 11-kDa peptide originally isolated from rat brain. In rat brain DBI coexists with at least three different processing products and the members of this peptide family have been shown to displace benzodiazepines and beta carbolines from recognition sit...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Neurochemical research 1990-02, Vol.15 (2), p.209-216
Hauptverfasser: ALHO, H, BOVOLIN, P, SLOBODYANSKY, E
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 216
container_issue 2
container_start_page 209
container_title Neurochemical research
container_volume 15
creator ALHO, H
BOVOLIN, P
SLOBODYANSKY, E
description Diazepam Binding Inhibitor (DBI) is an endogenous 11-kDa peptide originally isolated from rat brain. In rat brain DBI coexists with at least three different processing products and the members of this peptide family have been shown to displace benzodiazepines and beta carbolines from recognition sites located on the allosteric modulatory centers of GABAA receptors. Immunocytochemical methods were used to study the location of DBI and two of the processing products, octadecaneuropeptide (ODN) DBI 33-50 and triakontatetraneuropeptide (TTN) DBI 17-50, in rat brain. DBI-LI was found in selected neuronal perikarya and in many glia and glial-like cells. All circumventricular organs displayed a strong DBI like immunoreactivity (LI). The distribution and cellular location of the ODN-LI and TTN-LI differed from that of DBI because they were preferentially associated with DBI in neurons, but not in glia or glial-like cells. The presence of DBI, but not of its processing products, in glial cells, circumventricular organs, and cells of peripheral tissues suggests that the function of this peptide may extend to other yet unknown function in addition to an action on the allosteric modulatory center of GABAA receptors located in neurons.
doi_str_mv 10.1007/BF00972211
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79744675</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15591651</sourcerecordid><originalsourceid>FETCH-LOGICAL-c343t-1b4b5d763c5b1926075ba8c3e94eff0563d291f8838a3f2c5810b429a79a214b3</originalsourceid><addsrcrecordid>eNqFkD1PwzAURS0EKqWwsCN5AQFSwM-O7ZiNtnxUqsQCC0tkOw41apJiJwP8elK1oiPTG-65V08HoVMgN0CIvB0_EqIkpQB7aAhcskQowvbRkDCRJgwUOURHMX4S0uMUBmhAgSugYojep17_uJWusPF14esP7OuFN75tAr6cjmdXeBUa62JcR3fYV1VXNwsf28YuXOWtXuLYdoV3sS_iduFw0C02Qfv6GB2UehndyfaO0Nvjw-vkOZm_PM0m9_PEspS1CZjU8EIKZrkBRQWR3OjMMqdSV5aEC1ZQBWWWsUyzklqeATEpVVoqTSE1bIQuNrv9p1-di21e-Wjdcqlr13Qxl0qmqZD8XxB4L0Vw6MHrDWhDE2NwZb4KvtLhOweSr43nO-M9fLZd7Uzlij90q7jPz7e5jr2uMuja-rhbVIwLoIT9AktyhmM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15591651</pqid></control><display><type>article</type><title>Diazepam binding inhibitor (DBI) processing : immunohistochemical studies in the rat brain</title><source>MEDLINE</source><source>SpringerLink_现刊</source><creator>ALHO, H ; BOVOLIN, P ; SLOBODYANSKY, E</creator><creatorcontrib>ALHO, H ; BOVOLIN, P ; SLOBODYANSKY, E</creatorcontrib><description>Diazepam Binding Inhibitor (DBI) is an endogenous 11-kDa peptide originally isolated from rat brain. In rat brain DBI coexists with at least three different processing products and the members of this peptide family have been shown to displace benzodiazepines and beta carbolines from recognition sites located on the allosteric modulatory centers of GABAA receptors. Immunocytochemical methods were used to study the location of DBI and two of the processing products, octadecaneuropeptide (ODN) DBI 33-50 and triakontatetraneuropeptide (TTN) DBI 17-50, in rat brain. DBI-LI was found in selected neuronal perikarya and in many glia and glial-like cells. All circumventricular organs displayed a strong DBI like immunoreactivity (LI). The distribution and cellular location of the ODN-LI and TTN-LI differed from that of DBI because they were preferentially associated with DBI in neurons, but not in glia or glial-like cells. The presence of DBI, but not of its processing products, in glial cells, circumventricular organs, and cells of peripheral tissues suggests that the function of this peptide may extend to other yet unknown function in addition to an action on the allosteric modulatory center of GABAA receptors located in neurons.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1007/BF00972211</identifier><identifier>PMID: 2159126</identifier><identifier>CODEN: NEREDZ</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Animals ; Biochemistry and metabolism ; Biological and medical sciences ; Brain - cytology ; Brain - metabolism ; Central nervous system ; Diazepam Binding Inhibitor ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; Male ; Neuropeptides - metabolism ; Protein Precursors - metabolism ; Protein Processing, Post-Translational ; Rats ; Rats, Inbred Strains ; Receptors, GABA-A - drug effects ; Receptors, GABA-A - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Neurochemical research, 1990-02, Vol.15 (2), p.209-216</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-1b4b5d763c5b1926075ba8c3e94eff0563d291f8838a3f2c5810b429a79a214b3</citedby><cites>FETCH-LOGICAL-c343t-1b4b5d763c5b1926075ba8c3e94eff0563d291f8838a3f2c5810b429a79a214b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27928,27929</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=19356120$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2159126$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ALHO, H</creatorcontrib><creatorcontrib>BOVOLIN, P</creatorcontrib><creatorcontrib>SLOBODYANSKY, E</creatorcontrib><title>Diazepam binding inhibitor (DBI) processing : immunohistochemical studies in the rat brain</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><description>Diazepam Binding Inhibitor (DBI) is an endogenous 11-kDa peptide originally isolated from rat brain. In rat brain DBI coexists with at least three different processing products and the members of this peptide family have been shown to displace benzodiazepines and beta carbolines from recognition sites located on the allosteric modulatory centers of GABAA receptors. Immunocytochemical methods were used to study the location of DBI and two of the processing products, octadecaneuropeptide (ODN) DBI 33-50 and triakontatetraneuropeptide (TTN) DBI 17-50, in rat brain. DBI-LI was found in selected neuronal perikarya and in many glia and glial-like cells. All circumventricular organs displayed a strong DBI like immunoreactivity (LI). The distribution and cellular location of the ODN-LI and TTN-LI differed from that of DBI because they were preferentially associated with DBI in neurons, but not in glia or glial-like cells. The presence of DBI, but not of its processing products, in glial cells, circumventricular organs, and cells of peripheral tissues suggests that the function of this peptide may extend to other yet unknown function in addition to an action on the allosteric modulatory center of GABAA receptors located in neurons.</description><subject>Animals</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Brain - cytology</subject><subject>Brain - metabolism</subject><subject>Central nervous system</subject><subject>Diazepam Binding Inhibitor</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Neuropeptides - metabolism</subject><subject>Protein Precursors - metabolism</subject><subject>Protein Processing, Post-Translational</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Receptors, GABA-A - drug effects</subject><subject>Receptors, GABA-A - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0364-3190</issn><issn>1573-6903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAURS0EKqWwsCN5AQFSwM-O7ZiNtnxUqsQCC0tkOw41apJiJwP8elK1oiPTG-65V08HoVMgN0CIvB0_EqIkpQB7aAhcskQowvbRkDCRJgwUOURHMX4S0uMUBmhAgSugYojep17_uJWusPF14esP7OuFN75tAr6cjmdXeBUa62JcR3fYV1VXNwsf28YuXOWtXuLYdoV3sS_iduFw0C02Qfv6GB2UehndyfaO0Nvjw-vkOZm_PM0m9_PEspS1CZjU8EIKZrkBRQWR3OjMMqdSV5aEC1ZQBWWWsUyzklqeATEpVVoqTSE1bIQuNrv9p1-di21e-Wjdcqlr13Qxl0qmqZD8XxB4L0Vw6MHrDWhDE2NwZb4KvtLhOweSr43nO-M9fLZd7Uzlij90q7jPz7e5jr2uMuja-rhbVIwLoIT9AktyhmM</recordid><startdate>19900201</startdate><enddate>19900201</enddate><creator>ALHO, H</creator><creator>BOVOLIN, P</creator><creator>SLOBODYANSKY, E</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19900201</creationdate><title>Diazepam binding inhibitor (DBI) processing : immunohistochemical studies in the rat brain</title><author>ALHO, H ; BOVOLIN, P ; SLOBODYANSKY, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-1b4b5d763c5b1926075ba8c3e94eff0563d291f8838a3f2c5810b429a79a214b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Brain - cytology</topic><topic>Brain - metabolism</topic><topic>Central nervous system</topic><topic>Diazepam Binding Inhibitor</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Neuropeptides - metabolism</topic><topic>Protein Precursors - metabolism</topic><topic>Protein Processing, Post-Translational</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Receptors, GABA-A - drug effects</topic><topic>Receptors, GABA-A - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ALHO, H</creatorcontrib><creatorcontrib>BOVOLIN, P</creatorcontrib><creatorcontrib>SLOBODYANSKY, E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neurochemical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ALHO, H</au><au>BOVOLIN, P</au><au>SLOBODYANSKY, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diazepam binding inhibitor (DBI) processing : immunohistochemical studies in the rat brain</atitle><jtitle>Neurochemical research</jtitle><addtitle>Neurochem Res</addtitle><date>1990-02-01</date><risdate>1990</risdate><volume>15</volume><issue>2</issue><spage>209</spage><epage>216</epage><pages>209-216</pages><issn>0364-3190</issn><eissn>1573-6903</eissn><coden>NEREDZ</coden><abstract>Diazepam Binding Inhibitor (DBI) is an endogenous 11-kDa peptide originally isolated from rat brain. In rat brain DBI coexists with at least three different processing products and the members of this peptide family have been shown to displace benzodiazepines and beta carbolines from recognition sites located on the allosteric modulatory centers of GABAA receptors. Immunocytochemical methods were used to study the location of DBI and two of the processing products, octadecaneuropeptide (ODN) DBI 33-50 and triakontatetraneuropeptide (TTN) DBI 17-50, in rat brain. DBI-LI was found in selected neuronal perikarya and in many glia and glial-like cells. All circumventricular organs displayed a strong DBI like immunoreactivity (LI). The distribution and cellular location of the ODN-LI and TTN-LI differed from that of DBI because they were preferentially associated with DBI in neurons, but not in glia or glial-like cells. The presence of DBI, but not of its processing products, in glial cells, circumventricular organs, and cells of peripheral tissues suggests that the function of this peptide may extend to other yet unknown function in addition to an action on the allosteric modulatory center of GABAA receptors located in neurons.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>2159126</pmid><doi>10.1007/BF00972211</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0364-3190
ispartof Neurochemical research, 1990-02, Vol.15 (2), p.209-216
issn 0364-3190
1573-6903
language eng
recordid cdi_proquest_miscellaneous_79744675
source MEDLINE; SpringerLink_现刊
subjects Animals
Biochemistry and metabolism
Biological and medical sciences
Brain - cytology
Brain - metabolism
Central nervous system
Diazepam Binding Inhibitor
Fundamental and applied biological sciences. Psychology
Immunohistochemistry
Male
Neuropeptides - metabolism
Protein Precursors - metabolism
Protein Processing, Post-Translational
Rats
Rats, Inbred Strains
Receptors, GABA-A - drug effects
Receptors, GABA-A - metabolism
Vertebrates: nervous system and sense organs
title Diazepam binding inhibitor (DBI) processing : immunohistochemical studies in the rat brain
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-17T14%3A17%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Diazepam%20binding%20inhibitor%20(DBI)%20processing%20:%20immunohistochemical%20studies%20in%20the%20rat%20brain&rft.jtitle=Neurochemical%20research&rft.au=ALHO,%20H&rft.date=1990-02-01&rft.volume=15&rft.issue=2&rft.spage=209&rft.epage=216&rft.pages=209-216&rft.issn=0364-3190&rft.eissn=1573-6903&rft.coden=NEREDZ&rft_id=info:doi/10.1007/BF00972211&rft_dat=%3Cproquest_cross%3E15591651%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15591651&rft_id=info:pmid/2159126&rfr_iscdi=true