Protein C Activation by Recombinant Thrombomodulin in Plasma
Recombinant glycosaminoglycan-modified urinary thrombomodulin (GAG-UTM), which partially improved the amino acid sequence of human urinary thrombomodulin (UTM), was expressed in C127 cells. GAG-UTM accelerates protein C activation by thrombin and also thrombin inhibition by antithrombin III (ATIII)...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 1998/02/15, Vol.21(2), pp.177-179 |
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| container_title | Biological & pharmaceutical bulletin |
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| creator | EDANO, Toshiyuki KOMINE, Noriko YOSHIZAKI, Hideo OHKUCHI, Masao |
| description | Recombinant glycosaminoglycan-modified urinary thrombomodulin (GAG-UTM), which partially improved the amino acid sequence of human urinary thrombomodulin (UTM), was expressed in C127 cells. GAG-UTM accelerates protein C activation by thrombin and also thrombin inhibition by antithrombin III (ATIII) in the buffer system. Both accelerating activities of GAG-UTM are more potent than those of unmodified recombinant UTM (r-UTM) without a GAG chain. As ATIII in plasma also inhibits protein C activation by a thrombin-thrombomodulin complex, we studied whether GAG-UTM accelerates protein C activation in plasma. GAG-UTM suppressed the generation of thrombin in activating plasma protein C stronger than r-UTM. By Western blot analysis using anti-protein C antibody, activated protein C was generated by GAG-UTM more than by r-UTM. From these results, the acceleration of activated protein C formation by GAG-UTM was confirmed in plasma too. |
| doi_str_mv | 10.1248/bpb.21.177 |
| format | Article |
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GAG-UTM accelerates protein C activation by thrombin and also thrombin inhibition by antithrombin III (ATIII) in the buffer system. Both accelerating activities of GAG-UTM are more potent than those of unmodified recombinant UTM (r-UTM) without a GAG chain. As ATIII in plasma also inhibits protein C activation by a thrombin-thrombomodulin complex, we studied whether GAG-UTM accelerates protein C activation in plasma. GAG-UTM suppressed the generation of thrombin in activating plasma protein C stronger than r-UTM. By Western blot analysis using anti-protein C antibody, activated protein C was generated by GAG-UTM more than by r-UTM. From these results, the acceleration of activated protein C formation by GAG-UTM was confirmed in plasma too.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.21.177</identifier><identifier>PMID: 9514616</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>antithrombin III ; Biological and medical sciences ; Blood. Blood coagulation. Reticuloendothelial system ; Blotting, Western ; Cell Line ; Humans ; Medical sciences ; Micelles ; Pharmacology. Drug treatments ; protein C ; Protein C - metabolism ; protein C inhibitor ; Recombinant Proteins - pharmacology ; Thrombin - biosynthesis ; Thrombin - metabolism ; Thrombomodulin ; α1-antitrypsin</subject><ispartof>Biological and Pharmaceutical Bulletin, 1998/02/15, Vol.21(2), pp.177-179</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>1998 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c627t-83ee98d4c8df23c06cc26203e928126337b81b3052a8b8a243515d4f4d5cd20b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2208830$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9514616$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>EDANO, Toshiyuki</creatorcontrib><creatorcontrib>KOMINE, Noriko</creatorcontrib><creatorcontrib>YOSHIZAKI, Hideo</creatorcontrib><creatorcontrib>OHKUCHI, Masao</creatorcontrib><title>Protein C Activation by Recombinant Thrombomodulin in Plasma</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Recombinant glycosaminoglycan-modified urinary thrombomodulin (GAG-UTM), which partially improved the amino acid sequence of human urinary thrombomodulin (UTM), was expressed in C127 cells. GAG-UTM accelerates protein C activation by thrombin and also thrombin inhibition by antithrombin III (ATIII) in the buffer system. Both accelerating activities of GAG-UTM are more potent than those of unmodified recombinant UTM (r-UTM) without a GAG chain. As ATIII in plasma also inhibits protein C activation by a thrombin-thrombomodulin complex, we studied whether GAG-UTM accelerates protein C activation in plasma. GAG-UTM suppressed the generation of thrombin in activating plasma protein C stronger than r-UTM. By Western blot analysis using anti-protein C antibody, activated protein C was generated by GAG-UTM more than by r-UTM. From these results, the acceleration of activated protein C formation by GAG-UTM was confirmed in plasma too.</description><subject>antithrombin III</subject><subject>Biological and medical sciences</subject><subject>Blood. Blood coagulation. Reticuloendothelial system</subject><subject>Blotting, Western</subject><subject>Cell Line</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Micelles</subject><subject>Pharmacology. Drug treatments</subject><subject>protein C</subject><subject>Protein C - metabolism</subject><subject>protein C inhibitor</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Thrombin - biosynthesis</subject><subject>Thrombin - metabolism</subject><subject>Thrombomodulin</subject><subject>α1-antitrypsin</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkNFr2zAQxsXoaNNsL3svGFb2MHCqO8mWDH0JYd0GgZXSPQtJllcH28oku5D_vjIJeSiI08H34767j5AvQFeAXN6ZvVkhrECID2QBjIu8QCguyIJWIPMSCnlFrmPcUUoFRXZJLqsCeAnlgtw_Bj-6dsg22dqO7aseWz9k5pA9Oet70w56GLPnl5B63_t66hKa3mOnY68_kY-N7qL7fPqX5O_Dj-fNr3z75-fvzXqb2xLFmEvmXCVrbmXdILO0tBZLpMxVKAFLxoSRYBgtUEsjNXJWQFHzhteFrZEatiTfjnP3wf-fXBxV30bruk4Pzk9RiUpwJjhN4Nd34M5PYUi7KeC8AlHKdP-SfD9SNvgYg2vUPrS9DgcFVM2BqhSoQlAp0ATfnEZOpnf1GT0lmPTbk66j1V0T9GDbeMYQqZRs3mxzxHZx1P_cWddhbG3nZkeoKja74rEk87NqX3RQbmBvhmSS0w</recordid><startdate>19980201</startdate><enddate>19980201</enddate><creator>EDANO, Toshiyuki</creator><creator>KOMINE, Noriko</creator><creator>YOSHIZAKI, Hideo</creator><creator>OHKUCHI, Masao</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19980201</creationdate><title>Protein C Activation by Recombinant Thrombomodulin in Plasma</title><author>EDANO, Toshiyuki ; KOMINE, Noriko ; YOSHIZAKI, Hideo ; OHKUCHI, Masao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c627t-83ee98d4c8df23c06cc26203e928126337b81b3052a8b8a243515d4f4d5cd20b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>antithrombin III</topic><topic>Biological and medical sciences</topic><topic>Blood. Blood coagulation. Reticuloendothelial system</topic><topic>Blotting, Western</topic><topic>Cell Line</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Micelles</topic><topic>Pharmacology. Drug treatments</topic><topic>protein C</topic><topic>Protein C - metabolism</topic><topic>protein C inhibitor</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Thrombin - biosynthesis</topic><topic>Thrombin - metabolism</topic><topic>Thrombomodulin</topic><topic>α1-antitrypsin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>EDANO, Toshiyuki</creatorcontrib><creatorcontrib>KOMINE, Noriko</creatorcontrib><creatorcontrib>YOSHIZAKI, Hideo</creatorcontrib><creatorcontrib>OHKUCHI, Masao</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>EDANO, Toshiyuki</au><au>KOMINE, Noriko</au><au>YOSHIZAKI, Hideo</au><au>OHKUCHI, Masao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein C Activation by Recombinant Thrombomodulin in Plasma</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>1998-02-01</date><risdate>1998</risdate><volume>21</volume><issue>2</issue><spage>177</spage><epage>179</epage><pages>177-179</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>Recombinant glycosaminoglycan-modified urinary thrombomodulin (GAG-UTM), which partially improved the amino acid sequence of human urinary thrombomodulin (UTM), was expressed in C127 cells. GAG-UTM accelerates protein C activation by thrombin and also thrombin inhibition by antithrombin III (ATIII) in the buffer system. Both accelerating activities of GAG-UTM are more potent than those of unmodified recombinant UTM (r-UTM) without a GAG chain. As ATIII in plasma also inhibits protein C activation by a thrombin-thrombomodulin complex, we studied whether GAG-UTM accelerates protein C activation in plasma. GAG-UTM suppressed the generation of thrombin in activating plasma protein C stronger than r-UTM. By Western blot analysis using anti-protein C antibody, activated protein C was generated by GAG-UTM more than by r-UTM. From these results, the acceleration of activated protein C formation by GAG-UTM was confirmed in plasma too.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>9514616</pmid><doi>10.1248/bpb.21.177</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biological and Pharmaceutical Bulletin, 1998/02/15, Vol.21(2), pp.177-179 |
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| source | MEDLINE; EZB Free E-Journals; Free Full-Text Journals in Chemistry; J-STAGE |
| subjects | antithrombin III Biological and medical sciences Blood. Blood coagulation. Reticuloendothelial system Blotting, Western Cell Line Humans Medical sciences Micelles Pharmacology. Drug treatments protein C Protein C - metabolism protein C inhibitor Recombinant Proteins - pharmacology Thrombin - biosynthesis Thrombin - metabolism Thrombomodulin α1-antitrypsin |
| title | Protein C Activation by Recombinant Thrombomodulin in Plasma |
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