A Bacterial Enhancer Functions to Tether a Transcriptional Activator Near a Promoter
The nitrogen regulatory protein NtrC of enteric bacteria activates transcription of the glnA gene by catalyzing isomerization of closed complexes between RNA polymerase and the glnA promoter to open complexes. NtrC binds to sites upstream of glnA that have properties of eukaryotic transcriptional en...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1990-04, Vol.248 (4954), p.486-490 |
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| creator | Wedel, Andrew Weiss, David S. Popham, David Dröge, Peter Kustu, Sydney |
| description | The nitrogen regulatory protein NtrC of enteric bacteria activates transcription of the glnA gene by catalyzing isomerization of closed complexes between RNA polymerase and the glnA promoter to open complexes. NtrC binds to sites upstream of glnA that have properties of eukaryotic transcriptional enhancers. NtrC-binding sites were found to facilitate open complex formation when these sites and the glnA promoter were located on different rings of a singly linked catenane, but not when the two rings were decatenated. The results provide evidence that NtrC contacts RNA polymerase-promoter complexes in a process mediated by formation of a DNA loop. NtrC-binding sites serve to tether NtrC near the glnA promoter, thereby increasing the frequency of collisions between NtrC and polymerase-promoter complexes. |
| doi_str_mv | 10.1126/science.1970441 |
| format | Article |
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NtrC binds to sites upstream of glnA that have properties of eukaryotic transcriptional enhancers. NtrC-binding sites were found to facilitate open complex formation when these sites and the glnA promoter were located on different rings of a singly linked catenane, but not when the two rings were decatenated. The results provide evidence that NtrC contacts RNA polymerase-promoter complexes in a process mediated by formation of a DNA loop. NtrC-binding sites serve to tether NtrC near the glnA promoter, thereby increasing the frequency of collisions between NtrC and polymerase-promoter complexes.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1970441</identifier><identifier>PMID: 1970441</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Analytical, structural and metabolic biochemistry ; Bacteria ; Bacterial Proteins - metabolism ; Binding Sites ; Biochemical templates ; Biological and medical sciences ; Catenanes ; Deoxyribonucleic acid ; DNA ; DNA Transposable Elements ; DNA, Bacterial - metabolism ; DNA, Superhelical - metabolism ; DNA-Binding Proteins - metabolism ; DNA-Directed RNA Polymerases - metabolism ; Enhancer Elements, Genetic ; Fundamental and applied biological sciences. Psychology ; Gels ; General and theoretical biochemistry ; Genes ; Genetic aspects ; Genetic transcription ; Genetics ; Glutamate-Ammonia Ligase - genetics ; Laws, regulations and rules ; Molecules ; Nucleotidyltransferases - metabolism ; PII Nitrogen Regulatory Proteins ; Plasmids ; Promoter regions ; Promoter Regions, Genetic ; Promoters (Genetics) ; Proteins ; RNA ; RNA polymerases ; Templates, Genetic ; Trans-Activators ; Transcription (Genetics) ; Transcription Factors ; Transcription, Genetic ; Transposases ; Transposons</subject><ispartof>Science (American Association for the Advancement of Science), 1990-04, Vol.248 (4954), p.486-490</ispartof><rights>Copyright 1990 American Association for the Advancement of Science</rights><rights>1992 INIST-CNRS</rights><rights>COPYRIGHT 1990 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1990 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Apr 27, 1990</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c699t-6569889fccf53b639a2611f175ceca0d5e947cbabfd64fcb815a0ca24dbba87b3</citedby><cites>FETCH-LOGICAL-c699t-6569889fccf53b639a2611f175ceca0d5e947cbabfd64fcb815a0ca24dbba87b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2874322$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2874322$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5540639$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1970441$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wedel, Andrew</creatorcontrib><creatorcontrib>Weiss, David S.</creatorcontrib><creatorcontrib>Popham, David</creatorcontrib><creatorcontrib>Dröge, Peter</creatorcontrib><creatorcontrib>Kustu, Sydney</creatorcontrib><title>A Bacterial Enhancer Functions to Tether a Transcriptional Activator Near a Promoter</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>The nitrogen regulatory protein NtrC of enteric bacteria activates transcription of the glnA gene by catalyzing isomerization of closed complexes between RNA polymerase and the glnA promoter to open complexes. 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NtrC-binding sites serve to tether NtrC near the glnA promoter, thereby increasing the frequency of collisions between NtrC and polymerase-promoter complexes.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Bacteria</subject><subject>Bacterial Proteins - metabolism</subject><subject>Binding Sites</subject><subject>Biochemical templates</subject><subject>Biological and medical sciences</subject><subject>Catenanes</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Transposable Elements</subject><subject>DNA, Bacterial - metabolism</subject><subject>DNA, Superhelical - metabolism</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Enhancer Elements, Genetic</subject><subject>Fundamental and applied biological sciences. 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Sydney</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Bacterial Enhancer Functions to Tether a Transcriptional Activator Near a Promoter</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1990-04-27</date><risdate>1990</risdate><volume>248</volume><issue>4954</issue><spage>486</spage><epage>490</epage><pages>486-490</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>The nitrogen regulatory protein NtrC of enteric bacteria activates transcription of the glnA gene by catalyzing isomerization of closed complexes between RNA polymerase and the glnA promoter to open complexes. NtrC binds to sites upstream of glnA that have properties of eukaryotic transcriptional enhancers. NtrC-binding sites were found to facilitate open complex formation when these sites and the glnA promoter were located on different rings of a singly linked catenane, but not when the two rings were decatenated. The results provide evidence that NtrC contacts RNA polymerase-promoter complexes in a process mediated by formation of a DNA loop. NtrC-binding sites serve to tether NtrC near the glnA promoter, thereby increasing the frequency of collisions between NtrC and polymerase-promoter complexes.</abstract><cop>Washington, DC</cop><pub>American Society for the Advancement of Science</pub><pmid>1970441</pmid><doi>10.1126/science.1970441</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1990-04, Vol.248 (4954), p.486-490 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79742221 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; American Association for the Advancement of Science |
| subjects | Analytical, structural and metabolic biochemistry Bacteria Bacterial Proteins - metabolism Binding Sites Biochemical templates Biological and medical sciences Catenanes Deoxyribonucleic acid DNA DNA Transposable Elements DNA, Bacterial - metabolism DNA, Superhelical - metabolism DNA-Binding Proteins - metabolism DNA-Directed RNA Polymerases - metabolism Enhancer Elements, Genetic Fundamental and applied biological sciences. Psychology Gels General and theoretical biochemistry Genes Genetic aspects Genetic transcription Genetics Glutamate-Ammonia Ligase - genetics Laws, regulations and rules Molecules Nucleotidyltransferases - metabolism PII Nitrogen Regulatory Proteins Plasmids Promoter regions Promoter Regions, Genetic Promoters (Genetics) Proteins RNA RNA polymerases Templates, Genetic Trans-Activators Transcription (Genetics) Transcription Factors Transcription, Genetic Transposases Transposons |
| title | A Bacterial Enhancer Functions to Tether a Transcriptional Activator Near a Promoter |
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