Lack of effect following repeated in vivo exposure of the rabbit urinary bladder to urine from interstitial cystitis patients at low infusion volumes

We reported previously that substances in interstitial cystitis urine, when infused into the rabbit bladder, induce changes that resemble bladders of interstitial cystitis (IC) patients. Here we report our investigation of the effect of additional molecular weight subfractions of IC urine and lower...

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Veröffentlicht in:Neurourology and urodynamics 1998, Vol.17 (2), p.147-152
Hauptverfasser: Kohn, Ira J., Filer-Maerten, Sharon, Whitmore, Kristene E., Hanno, Philip M., Ruggieri, Michael R.
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container_end_page 152
container_issue 2
container_start_page 147
container_title Neurourology and urodynamics
container_volume 17
creator Kohn, Ira J.
Filer-Maerten, Sharon
Whitmore, Kristene E.
Hanno, Philip M.
Ruggieri, Michael R.
description We reported previously that substances in interstitial cystitis urine, when infused into the rabbit bladder, induce changes that resemble bladders of interstitial cystitis (IC) patients. Here we report our investigation of the effect of additional molecular weight subfractions of IC urine and lower infusion volume in this rabbit bladder bioassay. Urine was pooled from symptomatic IC patients, asymptomatic IC patients (in remission), and normal volunteers. Two fractions of 20× concentrated urine were obtained for each of the 3 groups: a 10–100‐kD fraction and a fraction > 100 kD but
doi_str_mv 10.1002/(SICI)1520-6777(1998)17:2<147::AID-NAU8>3.0.CO;2-D
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Here we report our investigation of the effect of additional molecular weight subfractions of IC urine and lower infusion volume in this rabbit bladder bioassay. Urine was pooled from symptomatic IC patients, asymptomatic IC patients (in remission), and normal volunteers. Two fractions of 20× concentrated urine were obtained for each of the 3 groups: a 10–100‐kD fraction and a fraction &gt; 100 kD but &lt;0.22 μm. Six rabbits per group were infused twice per week with 6 ml of 1 of these 6 urine fractions or saline as a control. After 6 weeks, each rabbit was cystoscoped before and after hydrodistension, bladder capacity and urea permeability were determined, and the bladder was removed for histologic examination. A questionnaire revealed a significant difference (P &lt; 0.01) regarding voiding symptom severity between symptomatic IC patients and both normal volunteers and IC patients in remission. There was no statistically significant difference among groups of rabbits in cystoscopic bladder appearance, bladder capacity, urea permeability, or bladder histology. If a urine‐borne factor is in part responsible for IC symptoms, the rabbit bladder must be filled with urine to near capacity to be able to detect a difference between IC and normal urine in this rabbit bladder bioassay. Neurourol. 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Urodyn</addtitle><description>We reported previously that substances in interstitial cystitis urine, when infused into the rabbit bladder, induce changes that resemble bladders of interstitial cystitis (IC) patients. Here we report our investigation of the effect of additional molecular weight subfractions of IC urine and lower infusion volume in this rabbit bladder bioassay. Urine was pooled from symptomatic IC patients, asymptomatic IC patients (in remission), and normal volunteers. Two fractions of 20× concentrated urine were obtained for each of the 3 groups: a 10–100‐kD fraction and a fraction &gt; 100 kD but &lt;0.22 μm. Six rabbits per group were infused twice per week with 6 ml of 1 of these 6 urine fractions or saline as a control. After 6 weeks, each rabbit was cystoscoped before and after hydrodistension, bladder capacity and urea permeability were determined, and the bladder was removed for histologic examination. A questionnaire revealed a significant difference (P &lt; 0.01) regarding voiding symptom severity between symptomatic IC patients and both normal volunteers and IC patients in remission. There was no statistically significant difference among groups of rabbits in cystoscopic bladder appearance, bladder capacity, urea permeability, or bladder histology. If a urine‐borne factor is in part responsible for IC symptoms, the rabbit bladder must be filled with urine to near capacity to be able to detect a difference between IC and normal urine in this rabbit bladder bioassay. Neurourol. 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subjects Administration, Intravesical
Animals
bladder distension
Compliance
Cystitis - urine
interstitial cystitis
Male
Rabbits
Urinary Bladder - physiology
urine
Urine - physiology
title Lack of effect following repeated in vivo exposure of the rabbit urinary bladder to urine from interstitial cystitis patients at low infusion volumes
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