Omental and subcutaneous adipose tissues of obese subjects release interleukin-6 : Depot difference and regulation by glucocorticoid
The purpose of this study was to determine whether human adipocytes from different depots of obese subjects produce interleukin-6 (IL-6) and whether IL-6 release is regulated by glucocorticoids. Fragments of omental and abdominal sc adipose tissue released immunodetectable IL-6 into the medium durin...
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| Veröffentlicht in: | The journal of clinical endocrinology and metabolism 1998-03, Vol.83 (3), p.847-850 |
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| container_issue | 3 |
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| container_title | The journal of clinical endocrinology and metabolism |
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| creator | FRIED, S. K BUNKIN, D. A GREENBERG, A. S |
| description | The purpose of this study was to determine whether human adipocytes from different depots of obese subjects produce interleukin-6 (IL-6) and whether IL-6 release is regulated by glucocorticoids. Fragments of omental and abdominal sc adipose tissue released immunodetectable IL-6 into the medium during acute incubations. Omental adipose tissue released 2-3 times more IL-6 than did sc adipose tissue. Isolated adipocytes prepared from these tissues also released IL-6 (omental > sc), but this accounted for only 10% of the total tissue release. Culture of adipose tissue fragments for 7 days with the glucocorticoid dexamethasone markedly suppressed IL-6 production. These data show for the first time that substantial quantities of IL-6 (up to 75 ng/mL) accumulate in the medium during incubations of both adipocytes and adipose tissue. Although little is known about the effects of IL-6 on adipose tissue, one action is a down-regulation of adipose tissue lipoprotein lipase. The regulated production of this multifunctional cytokine may modulate regional adipose tissue metabolism and may contribute to the recently reported correlation between serum IL-6 and the level of obesity. |
| doi_str_mv | 10.1210/jc.83.3.847 |
| format | Article |
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Although little is known about the effects of IL-6 on adipose tissue, one action is a down-regulation of adipose tissue lipoprotein lipase. The regulated production of this multifunctional cytokine may modulate regional adipose tissue metabolism and may contribute to the recently reported correlation between serum IL-6 and the level of obesity.</description><identifier>ISSN: 0021-972X</identifier><identifier>EISSN: 1945-7197</identifier><identifier>DOI: 10.1210/jc.83.3.847</identifier><identifier>PMID: 9506738</identifier><identifier>CODEN: JCEMAZ</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Adipocytes - drug effects ; Adipocytes - metabolism ; Adipose Tissue - metabolism ; Adipose Tissue - pathology ; Adult ; Biological and medical sciences ; Cells, Cultured ; Culture Media - metabolism ; Dexamethasone - pharmacology ; Female ; Glucocorticoids - pharmacology ; Humans ; Interleukin-6 - metabolism ; Male ; Medical sciences ; Metabolic diseases ; Obesity ; Obesity - metabolism ; Obesity - pathology ; Omentum ; Osmolar Concentration ; Skin ; Time Factors</subject><ispartof>The journal of clinical endocrinology and metabolism, 1998-03, Vol.83 (3), p.847-850</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-6e1bf00573c61c748d55d9cf47dfdd1bd5e7f51f7686de91bc14ecee51657bb03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2176003$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9506738$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FRIED, S. K</creatorcontrib><creatorcontrib>BUNKIN, D. A</creatorcontrib><creatorcontrib>GREENBERG, A. S</creatorcontrib><title>Omental and subcutaneous adipose tissues of obese subjects release interleukin-6 : Depot difference and regulation by glucocorticoid</title><title>The journal of clinical endocrinology and metabolism</title><addtitle>J Clin Endocrinol Metab</addtitle><description>The purpose of this study was to determine whether human adipocytes from different depots of obese subjects produce interleukin-6 (IL-6) and whether IL-6 release is regulated by glucocorticoids. Fragments of omental and abdominal sc adipose tissue released immunodetectable IL-6 into the medium during acute incubations. Omental adipose tissue released 2-3 times more IL-6 than did sc adipose tissue. Isolated adipocytes prepared from these tissues also released IL-6 (omental > sc), but this accounted for only 10% of the total tissue release. Culture of adipose tissue fragments for 7 days with the glucocorticoid dexamethasone markedly suppressed IL-6 production. These data show for the first time that substantial quantities of IL-6 (up to 75 ng/mL) accumulate in the medium during incubations of both adipocytes and adipose tissue. Although little is known about the effects of IL-6 on adipose tissue, one action is a down-regulation of adipose tissue lipoprotein lipase. The regulated production of this multifunctional cytokine may modulate regional adipose tissue metabolism and may contribute to the recently reported correlation between serum IL-6 and the level of obesity.</description><subject>Adipocytes - drug effects</subject><subject>Adipocytes - metabolism</subject><subject>Adipose Tissue - metabolism</subject><subject>Adipose Tissue - pathology</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Culture Media - metabolism</subject><subject>Dexamethasone - pharmacology</subject><subject>Female</subject><subject>Glucocorticoids - pharmacology</subject><subject>Humans</subject><subject>Interleukin-6 - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Obesity</subject><subject>Obesity - metabolism</subject><subject>Obesity - pathology</subject><subject>Omentum</subject><subject>Osmolar Concentration</subject><subject>Skin</subject><subject>Time Factors</subject><issn>0021-972X</issn><issn>1945-7197</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEGLFDEUhIMo6-zqybOQg3iRHpNJJ-n2JqurwsJeFLyF5OVlyZjpjEn6sHd_uNEZ9vSg6qMeVYS84mzLd5y938N2EluxnUb9hGz4PMpB81k_JRvGdnyY9e7nc3JZ654xPo5SXJCLWTKlxbQhf-4OuDSbqF08rauDtdkF81qp9fGYK9IWa12x0hxodtiFTu0RWqUFE9ouxKVhSbj-isug6Af6CY-5UR9DwIIL4P_sgvdrsi3mhboHep9WyJBLi5Cjf0GeBZsqvjzfK_Lj5vP366_D7d2Xb9cfbwcQem6DQu4CY1ILUBz0OHkp_Qxh1D54z52XqIPkQatJeZy5Az4iIEqupHaOiSvy9pR7LPl379TMIVbAlE6VjZ61YEyoDr47gVByrQWDOZZ4sOXBcGb-bW72YCZhhOmbd_r1OXZ1B_SP7Hnk7r85-7aCTaHYBWJ9xHZcq_5V_AUaUoy7</recordid><startdate>19980301</startdate><enddate>19980301</enddate><creator>FRIED, S. K</creator><creator>BUNKIN, D. A</creator><creator>GREENBERG, A. S</creator><general>Endocrine Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980301</creationdate><title>Omental and subcutaneous adipose tissues of obese subjects release interleukin-6 : Depot difference and regulation by glucocorticoid</title><author>FRIED, S. K ; BUNKIN, D. A ; GREENBERG, A. S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-6e1bf00573c61c748d55d9cf47dfdd1bd5e7f51f7686de91bc14ecee51657bb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adipocytes - drug effects</topic><topic>Adipocytes - metabolism</topic><topic>Adipose Tissue - metabolism</topic><topic>Adipose Tissue - pathology</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Culture Media - metabolism</topic><topic>Dexamethasone - pharmacology</topic><topic>Female</topic><topic>Glucocorticoids - pharmacology</topic><topic>Humans</topic><topic>Interleukin-6 - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>Obesity</topic><topic>Obesity - metabolism</topic><topic>Obesity - pathology</topic><topic>Omentum</topic><topic>Osmolar Concentration</topic><topic>Skin</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FRIED, S. K</creatorcontrib><creatorcontrib>BUNKIN, D. A</creatorcontrib><creatorcontrib>GREENBERG, A. S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of clinical endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FRIED, S. K</au><au>BUNKIN, D. A</au><au>GREENBERG, A. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Omental and subcutaneous adipose tissues of obese subjects release interleukin-6 : Depot difference and regulation by glucocorticoid</atitle><jtitle>The journal of clinical endocrinology and metabolism</jtitle><addtitle>J Clin Endocrinol Metab</addtitle><date>1998-03-01</date><risdate>1998</risdate><volume>83</volume><issue>3</issue><spage>847</spage><epage>850</epage><pages>847-850</pages><issn>0021-972X</issn><eissn>1945-7197</eissn><coden>JCEMAZ</coden><abstract>The purpose of this study was to determine whether human adipocytes from different depots of obese subjects produce interleukin-6 (IL-6) and whether IL-6 release is regulated by glucocorticoids. Fragments of omental and abdominal sc adipose tissue released immunodetectable IL-6 into the medium during acute incubations. Omental adipose tissue released 2-3 times more IL-6 than did sc adipose tissue. Isolated adipocytes prepared from these tissues also released IL-6 (omental > sc), but this accounted for only 10% of the total tissue release. Culture of adipose tissue fragments for 7 days with the glucocorticoid dexamethasone markedly suppressed IL-6 production. These data show for the first time that substantial quantities of IL-6 (up to 75 ng/mL) accumulate in the medium during incubations of both adipocytes and adipose tissue. Although little is known about the effects of IL-6 on adipose tissue, one action is a down-regulation of adipose tissue lipoprotein lipase. The regulated production of this multifunctional cytokine may modulate regional adipose tissue metabolism and may contribute to the recently reported correlation between serum IL-6 and the level of obesity.</abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>9506738</pmid><doi>10.1210/jc.83.3.847</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0021-972X |
| ispartof | The journal of clinical endocrinology and metabolism, 1998-03, Vol.83 (3), p.847-850 |
| issn | 0021-972X 1945-7197 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Adipocytes - drug effects Adipocytes - metabolism Adipose Tissue - metabolism Adipose Tissue - pathology Adult Biological and medical sciences Cells, Cultured Culture Media - metabolism Dexamethasone - pharmacology Female Glucocorticoids - pharmacology Humans Interleukin-6 - metabolism Male Medical sciences Metabolic diseases Obesity Obesity - metabolism Obesity - pathology Omentum Osmolar Concentration Skin Time Factors |
| title | Omental and subcutaneous adipose tissues of obese subjects release interleukin-6 : Depot difference and regulation by glucocorticoid |
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