Relationship of p53 molecular abnormalities with flow cytometry and growth factor receptor content in lung cancer
This study attempts to clarify the oncological significance of the p53 molecular abnormalities and p53 expression in lung cancer (LC) and their relationship with flow cytometry (FC) parameters and epidermal growth factor receptor (EGFR). The study includes 65 samples taken from both LC and normal lu...
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| Veröffentlicht in: | Clinica chimica acta 1998-01, Vol.269 (1), p.63-76 |
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| container_title | Clinica chimica acta |
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| creator | López-Guerrero, J.A Bolufer-Gilabert, P Marugán de la Concha, I Barragán-González, E Vera-Sempere, F.J |
| description | This study attempts to clarify the oncological significance of the p53 molecular abnormalities and p53 expression in lung cancer (LC) and their relationship with flow cytometry (FC) parameters and epidermal growth factor receptor (EGFR). The study includes 65 samples taken from both LC and normal lung (NL). The p53 molecular abnormalities of exons 4–8 were studied by single strand conformation polymorphisms (SSCP) and the loss of heterozygosity (LOH) of exon 4 by the Metzler method. P53 protein was detected by Western blot. EGFR was determined by a radioligand assay using [
125I]EGF. The FC parameters S phase fraction (SPF), DNA index (D.I.), G1G0 and growth rate (G2M+SPF) were evaluated from cellular monosuspensions. The LC with SSCP p53 molecular abnormalities have a significantly higher EGFR content (
P |
| doi_str_mv | 10.1016/S0009-8981(97)00186-1 |
| format | Article |
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125I]EGF. The FC parameters S phase fraction (SPF), DNA index (D.I.), G1G0 and growth rate (G2M+SPF) were evaluated from cellular monosuspensions. The LC with SSCP p53 molecular abnormalities have a significantly higher EGFR content (
P<0.001), SPF (
P<0.007), D.I. (
P<0.017) and a lower proportion of G1G0 cells (
P<0.04) than LC with no molecular abnormalities. No relationship between p53 molecular abnormalities and tumor TN or evolutive events was found. Neither the relationship between the molecular results and p53 expression detected by Western blot nor that of the p53 expression detected by Western with FC parameters or EGFR could be shown. In NL the growth fraction cells decrease significantly (
P<0.05) with the intensity of p53 expression. The lack of biological functionality of p53 with molecular abnormalities seemed to relate to fast growing LC whereas p53 expression detected by Western seemed more related to the wild type of p53.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/S0009-8981(97)00186-1</identifier><identifier>PMID: 9498104</identifier><identifier>CODEN: CCATAR</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Adult ; Aged ; Biological and medical sciences ; Blotting, Western ; Carcinoma, Non-Small-Cell Lung - genetics ; Carcinoma, Non-Small-Cell Lung - metabolism ; Carcinoma, Non-Small-Cell Lung - pathology ; Cell Division ; EGFR ; Epidermal growth factor receptor ; ErbB Receptors - metabolism ; Flow Cytometry ; Genes, p53 ; Humans ; LOH ; Loss of Heterozygosity ; Lung cancer ; Lung Neoplasms - genetics ; Lung Neoplasms - metabolism ; Lung Neoplasms - pathology ; Medical sciences ; Middle Aged ; Mutation ; p53 ; p53 Western blot ; Pneumology ; Polymorphism, Single-Stranded Conformational ; Single strand conformation polymorphisms ; SSCP ; Tumors of the respiratory system and mediastinum</subject><ispartof>Clinica chimica acta, 1998-01, Vol.269 (1), p.63-76</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-e0197deab6fca867a5cd648cbb57416b08c93e444c3f1dd030028e3ab0b5ebd83</citedby><cites>FETCH-LOGICAL-c389t-e0197deab6fca867a5cd648cbb57416b08c93e444c3f1dd030028e3ab0b5ebd83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0009898197001861$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2144922$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9498104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>López-Guerrero, J.A</creatorcontrib><creatorcontrib>Bolufer-Gilabert, P</creatorcontrib><creatorcontrib>Marugán de la Concha, I</creatorcontrib><creatorcontrib>Barragán-González, E</creatorcontrib><creatorcontrib>Vera-Sempere, F.J</creatorcontrib><title>Relationship of p53 molecular abnormalities with flow cytometry and growth factor receptor content in lung cancer</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>This study attempts to clarify the oncological significance of the p53 molecular abnormalities and p53 expression in lung cancer (LC) and their relationship with flow cytometry (FC) parameters and epidermal growth factor receptor (EGFR). The study includes 65 samples taken from both LC and normal lung (NL). The p53 molecular abnormalities of exons 4–8 were studied by single strand conformation polymorphisms (SSCP) and the loss of heterozygosity (LOH) of exon 4 by the Metzler method. P53 protein was detected by Western blot. EGFR was determined by a radioligand assay using [
125I]EGF. The FC parameters S phase fraction (SPF), DNA index (D.I.), G1G0 and growth rate (G2M+SPF) were evaluated from cellular monosuspensions. The LC with SSCP p53 molecular abnormalities have a significantly higher EGFR content (
P<0.001), SPF (
P<0.007), D.I. (
P<0.017) and a lower proportion of G1G0 cells (
P<0.04) than LC with no molecular abnormalities. No relationship between p53 molecular abnormalities and tumor TN or evolutive events was found. Neither the relationship between the molecular results and p53 expression detected by Western blot nor that of the p53 expression detected by Western with FC parameters or EGFR could be shown. In NL the growth fraction cells decrease significantly (
P<0.05) with the intensity of p53 expression. The lack of biological functionality of p53 with molecular abnormalities seemed to relate to fast growing LC whereas p53 expression detected by Western seemed more related to the wild type of p53.</description><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Carcinoma, Non-Small-Cell Lung - genetics</subject><subject>Carcinoma, Non-Small-Cell Lung - metabolism</subject><subject>Carcinoma, Non-Small-Cell Lung - pathology</subject><subject>Cell Division</subject><subject>EGFR</subject><subject>Epidermal growth factor receptor</subject><subject>ErbB Receptors - metabolism</subject><subject>Flow Cytometry</subject><subject>Genes, p53</subject><subject>Humans</subject><subject>LOH</subject><subject>Loss of Heterozygosity</subject><subject>Lung cancer</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - metabolism</subject><subject>Lung Neoplasms - pathology</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Mutation</subject><subject>p53</subject><subject>p53 Western blot</subject><subject>Pneumology</subject><subject>Polymorphism, Single-Stranded Conformational</subject><subject>Single strand conformation polymorphisms</subject><subject>SSCP</subject><subject>Tumors of the respiratory system and mediastinum</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv3CAURlHVKJ2m_QmRWFRVu3ACBtuwqqKoLylSpbRdIx7XCRU2DuCO5t-XyYxm2xWP79wLHBC6pOSKEtpf_ySEyEZIQT_I4SMhVPQNfYE2VAysYVy2L9HmhLxCr3P-U5ec9PQcnUteNwnfoKd7CLr4OOdHv-A44qVjeIoB7Bp0wtrMMU06-OIh460vj3gMcYvtrsQJStphPTv8kOJ2n2hbYsIJLCz7iY1zgblgP-Owzg_Y6tlCeoPORh0yvD2OF-j3l8-_br81dz--fr-9uWssE7I0QKgcHGjTj1aLftCddT0X1phu4LQ3RFjJgHNu2UidI4yQVgDThpgOjBPsAr0_9F1SfFohFzX5bCEEPUNcsxrk0A5d11ewO4A2xZwTjGpJftJppyhRe9XqWbXae1RyUM-qFa11l8cDVjOBO1Ud3db83THX2eowpvp8n09YS3n9pLZinw4YVBl_PSSVrYdqyvlqsigX_X8u8g9gjZ08</recordid><startdate>19980112</startdate><enddate>19980112</enddate><creator>López-Guerrero, J.A</creator><creator>Bolufer-Gilabert, P</creator><creator>Marugán de la Concha, I</creator><creator>Barragán-González, E</creator><creator>Vera-Sempere, F.J</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980112</creationdate><title>Relationship of p53 molecular abnormalities with flow cytometry and growth factor receptor content in lung cancer</title><author>López-Guerrero, J.A ; Bolufer-Gilabert, P ; Marugán de la Concha, I ; Barragán-González, E ; Vera-Sempere, F.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-e0197deab6fca867a5cd648cbb57416b08c93e444c3f1dd030028e3ab0b5ebd83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Carcinoma, Non-Small-Cell Lung - genetics</topic><topic>Carcinoma, Non-Small-Cell Lung - metabolism</topic><topic>Carcinoma, Non-Small-Cell Lung - pathology</topic><topic>Cell Division</topic><topic>EGFR</topic><topic>Epidermal growth factor receptor</topic><topic>ErbB Receptors - metabolism</topic><topic>Flow Cytometry</topic><topic>Genes, p53</topic><topic>Humans</topic><topic>LOH</topic><topic>Loss of Heterozygosity</topic><topic>Lung cancer</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - metabolism</topic><topic>Lung Neoplasms - pathology</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Mutation</topic><topic>p53</topic><topic>p53 Western blot</topic><topic>Pneumology</topic><topic>Polymorphism, Single-Stranded Conformational</topic><topic>Single strand conformation polymorphisms</topic><topic>SSCP</topic><topic>Tumors of the respiratory system and mediastinum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>López-Guerrero, J.A</creatorcontrib><creatorcontrib>Bolufer-Gilabert, P</creatorcontrib><creatorcontrib>Marugán de la Concha, I</creatorcontrib><creatorcontrib>Barragán-González, E</creatorcontrib><creatorcontrib>Vera-Sempere, F.J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>López-Guerrero, J.A</au><au>Bolufer-Gilabert, P</au><au>Marugán de la Concha, I</au><au>Barragán-González, E</au><au>Vera-Sempere, F.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationship of p53 molecular abnormalities with flow cytometry and growth factor receptor content in lung cancer</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1998-01-12</date><risdate>1998</risdate><volume>269</volume><issue>1</issue><spage>63</spage><epage>76</epage><pages>63-76</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><coden>CCATAR</coden><abstract>This study attempts to clarify the oncological significance of the p53 molecular abnormalities and p53 expression in lung cancer (LC) and their relationship with flow cytometry (FC) parameters and epidermal growth factor receptor (EGFR). The study includes 65 samples taken from both LC and normal lung (NL). The p53 molecular abnormalities of exons 4–8 were studied by single strand conformation polymorphisms (SSCP) and the loss of heterozygosity (LOH) of exon 4 by the Metzler method. P53 protein was detected by Western blot. EGFR was determined by a radioligand assay using [
125I]EGF. The FC parameters S phase fraction (SPF), DNA index (D.I.), G1G0 and growth rate (G2M+SPF) were evaluated from cellular monosuspensions. The LC with SSCP p53 molecular abnormalities have a significantly higher EGFR content (
P<0.001), SPF (
P<0.007), D.I. (
P<0.017) and a lower proportion of G1G0 cells (
P<0.04) than LC with no molecular abnormalities. No relationship between p53 molecular abnormalities and tumor TN or evolutive events was found. Neither the relationship between the molecular results and p53 expression detected by Western blot nor that of the p53 expression detected by Western with FC parameters or EGFR could be shown. In NL the growth fraction cells decrease significantly (
P<0.05) with the intensity of p53 expression. The lack of biological functionality of p53 with molecular abnormalities seemed to relate to fast growing LC whereas p53 expression detected by Western seemed more related to the wild type of p53.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>9498104</pmid><doi>10.1016/S0009-8981(97)00186-1</doi><tpages>14</tpages></addata></record> |
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| ispartof | Clinica chimica acta, 1998-01, Vol.269 (1), p.63-76 |
| issn | 0009-8981 1873-3492 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adult Aged Biological and medical sciences Blotting, Western Carcinoma, Non-Small-Cell Lung - genetics Carcinoma, Non-Small-Cell Lung - metabolism Carcinoma, Non-Small-Cell Lung - pathology Cell Division EGFR Epidermal growth factor receptor ErbB Receptors - metabolism Flow Cytometry Genes, p53 Humans LOH Loss of Heterozygosity Lung cancer Lung Neoplasms - genetics Lung Neoplasms - metabolism Lung Neoplasms - pathology Medical sciences Middle Aged Mutation p53 p53 Western blot Pneumology Polymorphism, Single-Stranded Conformational Single strand conformation polymorphisms SSCP Tumors of the respiratory system and mediastinum |
| title | Relationship of p53 molecular abnormalities with flow cytometry and growth factor receptor content in lung cancer |
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