The Structure of the Murine Calcium Channel γ-Subunit Gene and Protein
The gamma-subunit of voltage-gated calcium channels is a membrane protein that is associated with the skeletal muscle type of voltage gated calcium channels. Using a subunit-specific polyclonal antibody, the gamma-protein was detected in mouse skeletal muscle but not in brain, where at least five ad...
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| Veröffentlicht in: | Biological chemistry 1998, Vol.379 (1), p.45-50 |
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| creator | Wissenbach, Ulrich Bosse-Doenecke, Eva Freise, Doris Ludwig, Andreas Murakami, Manabu Hofmann, Franz Flockerzi, Veit |
| description | The gamma-subunit of voltage-gated calcium channels is a membrane protein that is associated with the skeletal muscle type of voltage gated calcium channels. Using a subunit-specific polyclonal antibody, the gamma-protein was detected in mouse skeletal muscle but not in brain, where at least five additional types of voltage-gated calcium channels are expressed. Murine genomic clones containing the full coding sequence of the gamma-subunit were isolated, the exons were mapped and sequenced. The murine gamma-subunit is encoded by a single copy gene containing 4 translated exons which are distributed over approximately 14 kilobases of DNA. The intron placement within the mouse gene correlates with the previously revealed organization of the human gamma-subunit gene, although the primary structures of the gamma-subunits are only moderately conserved between the murine, human, rat and rabbit proteins (75% identity). |
| doi_str_mv | 10.1515/bchm.1998.379.1.45 |
| format | Article |
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Using a subunit-specific polyclonal antibody, the gamma-protein was detected in mouse skeletal muscle but not in brain, where at least five additional types of voltage-gated calcium channels are expressed. Murine genomic clones containing the full coding sequence of the gamma-subunit were isolated, the exons were mapped and sequenced. The murine gamma-subunit is encoded by a single copy gene containing 4 translated exons which are distributed over approximately 14 kilobases of DNA. 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Using a subunit-specific polyclonal antibody, the gamma-protein was detected in mouse skeletal muscle but not in brain, where at least five additional types of voltage-gated calcium channels are expressed. Murine genomic clones containing the full coding sequence of the gamma-subunit were isolated, the exons were mapped and sequenced. The murine gamma-subunit is encoded by a single copy gene containing 4 translated exons which are distributed over approximately 14 kilobases of DNA. The intron placement within the mouse gene correlates with the previously revealed organization of the human gamma-subunit gene, although the primary structures of the gamma-subunits are only moderately conserved between the murine, human, rat and rabbit proteins (75% identity).</description><subject>Alternative Splicing - genetics</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Brain Chemistry</subject><subject>Calcium Channels - chemistry</subject><subject>Calcium Channels - genetics</subject><subject>Cloning, Molecular</subject><subject>Gene Expression - genetics</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Muscles - chemistry</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Restriction Mapping</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Transcription, Genetic - genetics</subject><issn>1431-6730</issn><issn>1437-4315</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctO3TAQhq2KigLtC1SqlFV3CZ7YjuNd0VF7QAJ6IXsrdiY9obmAL2p5Lt6jz1QfzhHLspoZ___8I30m5D3QAgSIU2M3UwFK1QWTqoCCi1fkCDiTOWcgDp56yCvJ6Bty7P0tpbSmnB2SQyUol1AdkXWzwewmuGhDdJgtfRbSw1V0w4zZqh3tEKdstWnnGcfs72N-E02ch5CtMent3GXf3BJwmN-S1307eny3ryek-fK5WZ3nl1_XF6uzy9zykodcohKiA1l1RtRKMQG06pmFvmRWGYYGTCdBlAppGi1FK4wFhUANk51kJ-TjLvbOLfcRfdDT4C2OYzvjEr2WSpZSlPWLRqg4LwWjyVjujNYt3jvs9Z0bptY9aKB6S1lvKestZZ0oa9BcpKUP-_RoJuyeV_ZYk_5pp_9ux4Cuw58uPqRG3y7RzYnP_8KlgqcT-S5i8AH_PF9o3S-dPlQK_b3h-pr-gPOm5lqwfwqMnEs</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>Wissenbach, Ulrich</creator><creator>Bosse-Doenecke, Eva</creator><creator>Freise, Doris</creator><creator>Ludwig, Andreas</creator><creator>Murakami, Manabu</creator><creator>Hofmann, Franz</creator><creator>Flockerzi, Veit</creator><general>Walter de Gruyter, Berlin / New York</general><general>De Gruyter</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1998</creationdate><title>The Structure of the Murine Calcium Channel γ-Subunit Gene and Protein</title><author>Wissenbach, Ulrich ; Bosse-Doenecke, Eva ; Freise, Doris ; Ludwig, Andreas ; Murakami, Manabu ; Hofmann, Franz ; Flockerzi, Veit</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-7e955d176db589935106f3c1f23c9b3eb1bd71529e09b3c0ec5bc19e10b37d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Alternative Splicing - genetics</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Brain Chemistry</topic><topic>Calcium Channels - chemistry</topic><topic>Calcium Channels - genetics</topic><topic>Cloning, Molecular</topic><topic>Gene Expression - genetics</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Muscles - chemistry</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Restriction Mapping</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Transcription, Genetic - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wissenbach, Ulrich</creatorcontrib><creatorcontrib>Bosse-Doenecke, Eva</creatorcontrib><creatorcontrib>Freise, Doris</creatorcontrib><creatorcontrib>Ludwig, Andreas</creatorcontrib><creatorcontrib>Murakami, Manabu</creatorcontrib><creatorcontrib>Hofmann, Franz</creatorcontrib><creatorcontrib>Flockerzi, Veit</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wissenbach, Ulrich</au><au>Bosse-Doenecke, Eva</au><au>Freise, Doris</au><au>Ludwig, Andreas</au><au>Murakami, Manabu</au><au>Hofmann, Franz</au><au>Flockerzi, Veit</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Structure of the Murine Calcium Channel γ-Subunit Gene and Protein</atitle><jtitle>Biological chemistry</jtitle><addtitle>Biological Chemistry</addtitle><date>1998</date><risdate>1998</risdate><volume>379</volume><issue>1</issue><spage>45</spage><epage>50</epage><pages>45-50</pages><issn>1431-6730</issn><eissn>1437-4315</eissn><abstract>The gamma-subunit of voltage-gated calcium channels is a membrane protein that is associated with the skeletal muscle type of voltage gated calcium channels. Using a subunit-specific polyclonal antibody, the gamma-protein was detected in mouse skeletal muscle but not in brain, where at least five additional types of voltage-gated calcium channels are expressed. Murine genomic clones containing the full coding sequence of the gamma-subunit were isolated, the exons were mapped and sequenced. The murine gamma-subunit is encoded by a single copy gene containing 4 translated exons which are distributed over approximately 14 kilobases of DNA. The intron placement within the mouse gene correlates with the previously revealed organization of the human gamma-subunit gene, although the primary structures of the gamma-subunits are only moderately conserved between the murine, human, rat and rabbit proteins (75% identity).</abstract><cop>Berlin, New York</cop><pub>Walter de Gruyter, Berlin / New York</pub><pmid>9504716</pmid><doi>10.1515/bchm.1998.379.1.45</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biological chemistry, 1998, Vol.379 (1), p.45-50 |
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| language | eng |
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| source | MEDLINE; De Gruyter journals |
| subjects | Alternative Splicing - genetics Amino Acid Sequence Animals Base Sequence Brain Chemistry Calcium Channels - chemistry Calcium Channels - genetics Cloning, Molecular Gene Expression - genetics Mice Molecular Sequence Data Muscles - chemistry Recombinant Fusion Proteins - genetics Restriction Mapping Sequence Analysis, DNA Sequence Homology, Amino Acid Transcription, Genetic - genetics |
| title | The Structure of the Murine Calcium Channel γ-Subunit Gene and Protein |
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