Tetra-amidines exhibiting anti-proteinase activity: effects on oriented migration and in vitro invasiveness of a Chinese Hamster cell line transfected with the activated human T24-Ha-ras-1 oncogene
In the present paper we have investigated the effects of aromatic tetra-amidines on attachment, oriented migration and in vitro invasiveness of the Chinese Hamster FH06T1-1 fibroblast lung cell line, transformed with the activated human T24-Ha-ras-1 oncogene. The FH06T1-1 cell line is tumorigenic in...
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| Veröffentlicht in: | Cancer letters 1990-04, Vol.50 (2), p.93-102 |
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| creator | Nastruzzi, C. Barbieri, R. Ferroni, R. Guarneri, M. Spandidos, D. Gambari, R. |
| description | In the present paper we have investigated the effects of aromatic tetra-amidines on attachment, oriented migration and in vitro invasiveness of the Chinese Hamster FH06T1-1 fibroblast lung cell line, transformed with the activated human T24-Ha-ras-1 oncogene.
The FH06T1-1 cell line is tumorigenic in nude mice and displays growth properties and biological features clearly distinct from those of the FH06N1-1 cell line, obtained after transfection of the same fibroblast cells with the normal Ha-ras-1 proto-oncogene.
Attachment, oriented migration and invasiveness were analysed by culturing the cells on a reconstituted extracellular matrix, composed of collagen IV, laminin, entactin and heparan sulphate proteoglycans.
The results obtained demonstrate that oriented migration is performed only by FH06T1-1 cells and that tetra-benzamidines are effective inhibitors of oriented migration and “in vitro” invasiveness of these tumorigenic cells. These findings should encourage further studies on the possible antimetastatic effects of these antiproteinase tetra-benzamidines on experimental animals. |
| doi_str_mv | 10.1016/0304-3835(90)90237-R |
| format | Article |
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The FH06T1-1 cell line is tumorigenic in nude mice and displays growth properties and biological features clearly distinct from those of the FH06N1-1 cell line, obtained after transfection of the same fibroblast cells with the normal Ha-ras-1 proto-oncogene.
Attachment, oriented migration and invasiveness were analysed by culturing the cells on a reconstituted extracellular matrix, composed of collagen IV, laminin, entactin and heparan sulphate proteoglycans.
The results obtained demonstrate that oriented migration is performed only by FH06T1-1 cells and that tetra-benzamidines are effective inhibitors of oriented migration and “in vitro” invasiveness of these tumorigenic cells. These findings should encourage further studies on the possible antimetastatic effects of these antiproteinase tetra-benzamidines on experimental animals.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/0304-3835(90)90237-R</identifier><identifier>PMID: 2183928</identifier><identifier>CODEN: CALEDQ</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Amidines - pharmacology ; Animals ; Antineoplastic Agents ; Benzamidines - pharmacology ; Biological and medical sciences ; Cell Division - drug effects ; Cell Movement - drug effects ; Chemical Phenomena ; Chemistry ; Cricetinae ; Cricetulus ; Depression, Chemical ; extracellular matrix ; Gene Expression Regulation, Neoplastic - drug effects ; General aspects ; Genes, ras - drug effects ; Humans ; Medical sciences ; Neoplasm Invasiveness ; Oncogene Protein p21(ras) - biosynthesis ; oriented migration ; Pharmacology. Drug treatments ; Protease Inhibitors ; proteinase inhibitor ; Transfection ; Tumor Cells, Cultured - drug effects ; Tumor Cells, Cultured - metabolism ; Tumor Cells, Cultured - pathology ; tumor invasion</subject><ispartof>Cancer letters, 1990-04, Vol.50 (2), p.93-102</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c333t-13ba710b973a0a172cbe4c6783b9b93199a9a45124545d144f1b650ae145a6d73</citedby><cites>FETCH-LOGICAL-c333t-13ba710b973a0a172cbe4c6783b9b93199a9a45124545d144f1b650ae145a6d73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0304-3835(90)90237-R$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19834909$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2183928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nastruzzi, C.</creatorcontrib><creatorcontrib>Barbieri, R.</creatorcontrib><creatorcontrib>Ferroni, R.</creatorcontrib><creatorcontrib>Guarneri, M.</creatorcontrib><creatorcontrib>Spandidos, D.</creatorcontrib><creatorcontrib>Gambari, R.</creatorcontrib><title>Tetra-amidines exhibiting anti-proteinase activity: effects on oriented migration and in vitro invasiveness of a Chinese Hamster cell line transfected with the activated human T24-Ha-ras-1 oncogene</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>In the present paper we have investigated the effects of aromatic tetra-amidines on attachment, oriented migration and in vitro invasiveness of the Chinese Hamster FH06T1-1 fibroblast lung cell line, transformed with the activated human T24-Ha-ras-1 oncogene.
The FH06T1-1 cell line is tumorigenic in nude mice and displays growth properties and biological features clearly distinct from those of the FH06N1-1 cell line, obtained after transfection of the same fibroblast cells with the normal Ha-ras-1 proto-oncogene.
Attachment, oriented migration and invasiveness were analysed by culturing the cells on a reconstituted extracellular matrix, composed of collagen IV, laminin, entactin and heparan sulphate proteoglycans.
The results obtained demonstrate that oriented migration is performed only by FH06T1-1 cells and that tetra-benzamidines are effective inhibitors of oriented migration and “in vitro” invasiveness of these tumorigenic cells. These findings should encourage further studies on the possible antimetastatic effects of these antiproteinase tetra-benzamidines on experimental animals.</description><subject>Amidines - pharmacology</subject><subject>Animals</subject><subject>Antineoplastic Agents</subject><subject>Benzamidines - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell Division - drug effects</subject><subject>Cell Movement - drug effects</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Depression, Chemical</subject><subject>extracellular matrix</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>General aspects</subject><subject>Genes, ras - drug effects</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Neoplasm Invasiveness</subject><subject>Oncogene Protein p21(ras) - biosynthesis</subject><subject>oriented migration</subject><subject>Pharmacology. Drug treatments</subject><subject>Protease Inhibitors</subject><subject>proteinase inhibitor</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured - drug effects</subject><subject>Tumor Cells, Cultured - metabolism</subject><subject>Tumor Cells, Cultured - pathology</subject><subject>tumor invasion</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd-K1DAUxoso67j6Bgq5UfQimjRp03ghyKCOsCAs43U4TU-nkTZdk8zoPqDvZeqU9U6vEs75ne_8-YriKWevOeP1GyaYpKIR1UvNXmlWCkWv7xUb3qiSKt2w-8XmDnlYPIrxG2Oskqq6KC5K3ghdNpvi1x5TAAqT65zHSPDn4FqXnD8Q8MnRmzAndB4iErDJnVy6fUuw79GmSGZP5uDQJ-zI5A4Bkssh8B1xnmQ0zPlzguhOmLUz3xMg22FphGQHU0wYiMVxJGOOkTyIj4tylvvh0kDSsHaFJTQcJ_BkX0q6AxogUp4HsPMhaz8uHvQwRnyyvpfF148f9tsdvfry6fP2_RW1QohEuWhBcdZqJYABV6VtUdpaNaLVrRZca9AgK17KSlYdl7LnbV0xQC4rqDslLosXZ918lu9HjMlMLi4LgMf5GI3SqqwVl_8FeVWrWlcsg_IM2jDHGLA3N8FNEG4NZ2ax2SwemsVDo5n5Y7O5zmXPVv1jO2F3V7T6mvPP1zxEC2OfL2td_KutGyE105l7d-YwX-3kMJhos6EWOxeyEaab3b8H-Q0lt8bj</recordid><startdate>19900420</startdate><enddate>19900420</enddate><creator>Nastruzzi, C.</creator><creator>Barbieri, R.</creator><creator>Ferroni, R.</creator><creator>Guarneri, M.</creator><creator>Spandidos, D.</creator><creator>Gambari, R.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19900420</creationdate><title>Tetra-amidines exhibiting anti-proteinase activity: effects on oriented migration and in vitro invasiveness of a Chinese Hamster cell line transfected with the activated human T24-Ha-ras-1 oncogene</title><author>Nastruzzi, C. ; Barbieri, R. ; Ferroni, R. ; Guarneri, M. ; Spandidos, D. ; Gambari, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c333t-13ba710b973a0a172cbe4c6783b9b93199a9a45124545d144f1b650ae145a6d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amidines - pharmacology</topic><topic>Animals</topic><topic>Antineoplastic Agents</topic><topic>Benzamidines - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell Division - drug effects</topic><topic>Cell Movement - drug effects</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>Depression, Chemical</topic><topic>extracellular matrix</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>General aspects</topic><topic>Genes, ras - drug effects</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Neoplasm Invasiveness</topic><topic>Oncogene Protein p21(ras) - biosynthesis</topic><topic>oriented migration</topic><topic>Pharmacology. Drug treatments</topic><topic>Protease Inhibitors</topic><topic>proteinase inhibitor</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured - drug effects</topic><topic>Tumor Cells, Cultured - metabolism</topic><topic>Tumor Cells, Cultured - pathology</topic><topic>tumor invasion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nastruzzi, C.</creatorcontrib><creatorcontrib>Barbieri, R.</creatorcontrib><creatorcontrib>Ferroni, R.</creatorcontrib><creatorcontrib>Guarneri, M.</creatorcontrib><creatorcontrib>Spandidos, D.</creatorcontrib><creatorcontrib>Gambari, R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nastruzzi, C.</au><au>Barbieri, R.</au><au>Ferroni, R.</au><au>Guarneri, M.</au><au>Spandidos, D.</au><au>Gambari, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tetra-amidines exhibiting anti-proteinase activity: effects on oriented migration and in vitro invasiveness of a Chinese Hamster cell line transfected with the activated human T24-Ha-ras-1 oncogene</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>1990-04-20</date><risdate>1990</risdate><volume>50</volume><issue>2</issue><spage>93</spage><epage>102</epage><pages>93-102</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><coden>CALEDQ</coden><abstract>In the present paper we have investigated the effects of aromatic tetra-amidines on attachment, oriented migration and in vitro invasiveness of the Chinese Hamster FH06T1-1 fibroblast lung cell line, transformed with the activated human T24-Ha-ras-1 oncogene.
The FH06T1-1 cell line is tumorigenic in nude mice and displays growth properties and biological features clearly distinct from those of the FH06N1-1 cell line, obtained after transfection of the same fibroblast cells with the normal Ha-ras-1 proto-oncogene.
Attachment, oriented migration and invasiveness were analysed by culturing the cells on a reconstituted extracellular matrix, composed of collagen IV, laminin, entactin and heparan sulphate proteoglycans.
The results obtained demonstrate that oriented migration is performed only by FH06T1-1 cells and that tetra-benzamidines are effective inhibitors of oriented migration and “in vitro” invasiveness of these tumorigenic cells. These findings should encourage further studies on the possible antimetastatic effects of these antiproteinase tetra-benzamidines on experimental animals.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>2183928</pmid><doi>10.1016/0304-3835(90)90237-R</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0304-3835 |
| ispartof | Cancer letters, 1990-04, Vol.50 (2), p.93-102 |
| issn | 0304-3835 1872-7980 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Amidines - pharmacology Animals Antineoplastic Agents Benzamidines - pharmacology Biological and medical sciences Cell Division - drug effects Cell Movement - drug effects Chemical Phenomena Chemistry Cricetinae Cricetulus Depression, Chemical extracellular matrix Gene Expression Regulation, Neoplastic - drug effects General aspects Genes, ras - drug effects Humans Medical sciences Neoplasm Invasiveness Oncogene Protein p21(ras) - biosynthesis oriented migration Pharmacology. Drug treatments Protease Inhibitors proteinase inhibitor Transfection Tumor Cells, Cultured - drug effects Tumor Cells, Cultured - metabolism Tumor Cells, Cultured - pathology tumor invasion |
| title | Tetra-amidines exhibiting anti-proteinase activity: effects on oriented migration and in vitro invasiveness of a Chinese Hamster cell line transfected with the activated human T24-Ha-ras-1 oncogene |
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