Quinolinic acid protects rat cerebellar granule cells from glutamate-induced apoptosis
The effects of quinolinic acid (QUIN) on glutamate-induced excitotoxicity were examined in primary cultures of rat cerebellar granule neurons. Exposing these neurons to QUIN (≤2.5 mM) in the presence of glucose and Mg 2+ had no effect on their viability. Although pretreating neurons with QUIN (10 μM...
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| Veröffentlicht in: | Neuroscience letters 1998-01, Vol.241 (2), p.180-184 |
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| creator | Sei, Yoshitatsu Fossom, Linda Goping, Gertrud Skolnick, Phil Basile, Anthony S |
| description | The effects of quinolinic acid (QUIN) on glutamate-induced excitotoxicity were examined in primary cultures of rat cerebellar granule neurons. Exposing these neurons to QUIN (≤2.5 mM) in the presence of glucose and Mg
2+ had no effect on their viability. Although pretreating neurons with QUIN (10
μM) for 6 h did not reduce necrotic death induced by glutamate exposure in the absence of glucose and Mg
2+, QUIN pretreatment significantly suppressed glutamate-induced apoptosis by 68% (as indicated by DNA fragmentation) in cultures containing glucose and Mg
2+. Furthermore, the
N-methyl-
d-aspartate (NMDA) receptor antagonist AP-5 reversed QUIN-induced neuroprotection, while the non-NMDA antagonist CNQX had no effect. This study demonstrates that pathophysiologically relevant concentrations of QUIN can protect neurons from apoptosis mediated via the NMDA receptor. |
| doi_str_mv | 10.1016/S0304-3940(97)00980-4 |
| format | Article |
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2+ had no effect on their viability. Although pretreating neurons with QUIN (10
μM) for 6 h did not reduce necrotic death induced by glutamate exposure in the absence of glucose and Mg
2+, QUIN pretreatment significantly suppressed glutamate-induced apoptosis by 68% (as indicated by DNA fragmentation) in cultures containing glucose and Mg
2+. Furthermore, the
N-methyl-
d-aspartate (NMDA) receptor antagonist AP-5 reversed QUIN-induced neuroprotection, while the non-NMDA antagonist CNQX had no effect. This study demonstrates that pathophysiologically relevant concentrations of QUIN can protect neurons from apoptosis mediated via the NMDA receptor.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/S0304-3940(97)00980-4</identifier><identifier>PMID: 9507950</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Apoptosis ; Apoptosis - drug effects ; Biological and medical sciences ; Cell death ; Cerebellum - cytology ; Cerebellum - drug effects ; Cerebellum - metabolism ; Excitatory Amino Acid Agonists - pharmacology ; Excitatory Amino Acid Antagonists - pharmacology ; Fundamental and applied biological sciences. Psychology ; Glutamate ; Glutamic Acid - pharmacology ; Granule neuron ; Isolated neuron and nerve. Neuroglia ; Logistic Models ; N-Methylaspartate - pharmacology ; Necrosis ; Neurons - drug effects ; Neuroprotective Agents - pharmacology ; Neurotoxicity ; Quinolinic acid ; Quinolinic Acid - pharmacology ; Rats ; Rats, Sprague-Dawley ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 1998-01, Vol.241 (2), p.180-184</ispartof><rights>1998 Elsevier Science Ireland Ltd</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-57125dc59668a06a56ba26d1f406eea8498b102c866c3b2bfefb86e05678c0213</citedby><cites>FETCH-LOGICAL-c420t-57125dc59668a06a56ba26d1f406eea8498b102c866c3b2bfefb86e05678c0213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304394097009804$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2157192$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9507950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sei, Yoshitatsu</creatorcontrib><creatorcontrib>Fossom, Linda</creatorcontrib><creatorcontrib>Goping, Gertrud</creatorcontrib><creatorcontrib>Skolnick, Phil</creatorcontrib><creatorcontrib>Basile, Anthony S</creatorcontrib><title>Quinolinic acid protects rat cerebellar granule cells from glutamate-induced apoptosis</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>The effects of quinolinic acid (QUIN) on glutamate-induced excitotoxicity were examined in primary cultures of rat cerebellar granule neurons. Exposing these neurons to QUIN (≤2.5 mM) in the presence of glucose and Mg
2+ had no effect on their viability. Although pretreating neurons with QUIN (10
μM) for 6 h did not reduce necrotic death induced by glutamate exposure in the absence of glucose and Mg
2+, QUIN pretreatment significantly suppressed glutamate-induced apoptosis by 68% (as indicated by DNA fragmentation) in cultures containing glucose and Mg
2+. Furthermore, the
N-methyl-
d-aspartate (NMDA) receptor antagonist AP-5 reversed QUIN-induced neuroprotection, while the non-NMDA antagonist CNQX had no effect. This study demonstrates that pathophysiologically relevant concentrations of QUIN can protect neurons from apoptosis mediated via the NMDA receptor.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Cell death</subject><subject>Cerebellum - cytology</subject><subject>Cerebellum - drug effects</subject><subject>Cerebellum - metabolism</subject><subject>Excitatory Amino Acid Agonists - pharmacology</subject><subject>Excitatory Amino Acid Antagonists - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutamate</subject><subject>Glutamic Acid - pharmacology</subject><subject>Granule neuron</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>Logistic Models</subject><subject>N-Methylaspartate - pharmacology</subject><subject>Necrosis</subject><subject>Neurons - drug effects</subject><subject>Neuroprotective Agents - pharmacology</subject><subject>Neurotoxicity</subject><subject>Quinolinic acid</subject><subject>Quinolinic Acid - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1L5TAQhoMoetbdnyD0QsS96DpJ89Fcich-gSCiu7chTacSSdtj0gr-e1PP4dyeixDIPJN5eYaQMwo_KFB59QgV8LLSHC61-g6gayj5AVnRWrFSacUOyWqHnJAvKb0AgKCCH5NjLUDlsyL_H2Y_jMEP3hXW-bZYx3FCN6Ui2qlwGLHBEGwsnqMd5oD5KYRUdHHsi-cwT7a3E5Z-aGeHbWHX43oak09fyVFnQ8Jv2_uU_Pv18-n2T3l3__vv7c1d6TiDqRSKMtE6oaWsLUgrZGOZbGnHQSLamuu6ocBcLaWrGtZ02DW1RBBS1Q4YrU7JxebfHPt1xjSZ3qcloh1wnJNZPEiQai9IZaUl5SyDYgO6OKYUsTPr6Hsb3w0Fs4g3n-LNYtVoZT7FG577zrYD5qbHdte1NZ3r59u6Tc6GLut0Pu0wRrMLvYy_3mCYrb15jCY5j0OW62Nei2lHvyfIB84jn4I</recordid><startdate>19980130</startdate><enddate>19980130</enddate><creator>Sei, Yoshitatsu</creator><creator>Fossom, Linda</creator><creator>Goping, Gertrud</creator><creator>Skolnick, Phil</creator><creator>Basile, Anthony S</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19980130</creationdate><title>Quinolinic acid protects rat cerebellar granule cells from glutamate-induced apoptosis</title><author>Sei, Yoshitatsu ; Fossom, Linda ; Goping, Gertrud ; Skolnick, Phil ; Basile, Anthony S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-57125dc59668a06a56ba26d1f406eea8498b102c866c3b2bfefb86e05678c0213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Biological and medical sciences</topic><topic>Cell death</topic><topic>Cerebellum - cytology</topic><topic>Cerebellum - drug effects</topic><topic>Cerebellum - metabolism</topic><topic>Excitatory Amino Acid Agonists - pharmacology</topic><topic>Excitatory Amino Acid Antagonists - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutamate</topic><topic>Glutamic Acid - pharmacology</topic><topic>Granule neuron</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>Logistic Models</topic><topic>N-Methylaspartate - pharmacology</topic><topic>Necrosis</topic><topic>Neurons - drug effects</topic><topic>Neuroprotective Agents - pharmacology</topic><topic>Neurotoxicity</topic><topic>Quinolinic acid</topic><topic>Quinolinic Acid - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sei, Yoshitatsu</creatorcontrib><creatorcontrib>Fossom, Linda</creatorcontrib><creatorcontrib>Goping, Gertrud</creatorcontrib><creatorcontrib>Skolnick, Phil</creatorcontrib><creatorcontrib>Basile, Anthony S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sei, Yoshitatsu</au><au>Fossom, Linda</au><au>Goping, Gertrud</au><au>Skolnick, Phil</au><au>Basile, Anthony S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quinolinic acid protects rat cerebellar granule cells from glutamate-induced apoptosis</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1998-01-30</date><risdate>1998</risdate><volume>241</volume><issue>2</issue><spage>180</spage><epage>184</epage><pages>180-184</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>The effects of quinolinic acid (QUIN) on glutamate-induced excitotoxicity were examined in primary cultures of rat cerebellar granule neurons. Exposing these neurons to QUIN (≤2.5 mM) in the presence of glucose and Mg
2+ had no effect on their viability. Although pretreating neurons with QUIN (10
μM) for 6 h did not reduce necrotic death induced by glutamate exposure in the absence of glucose and Mg
2+, QUIN pretreatment significantly suppressed glutamate-induced apoptosis by 68% (as indicated by DNA fragmentation) in cultures containing glucose and Mg
2+. Furthermore, the
N-methyl-
d-aspartate (NMDA) receptor antagonist AP-5 reversed QUIN-induced neuroprotection, while the non-NMDA antagonist CNQX had no effect. This study demonstrates that pathophysiologically relevant concentrations of QUIN can protect neurons from apoptosis mediated via the NMDA receptor.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>9507950</pmid><doi>10.1016/S0304-3940(97)00980-4</doi><tpages>5</tpages></addata></record> |
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| ispartof | Neuroscience letters, 1998-01, Vol.241 (2), p.180-184 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Apoptosis Apoptosis - drug effects Biological and medical sciences Cell death Cerebellum - cytology Cerebellum - drug effects Cerebellum - metabolism Excitatory Amino Acid Agonists - pharmacology Excitatory Amino Acid Antagonists - pharmacology Fundamental and applied biological sciences. Psychology Glutamate Glutamic Acid - pharmacology Granule neuron Isolated neuron and nerve. Neuroglia Logistic Models N-Methylaspartate - pharmacology Necrosis Neurons - drug effects Neuroprotective Agents - pharmacology Neurotoxicity Quinolinic acid Quinolinic Acid - pharmacology Rats Rats, Sprague-Dawley Vertebrates: nervous system and sense organs |
| title | Quinolinic acid protects rat cerebellar granule cells from glutamate-induced apoptosis |
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